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J Exp Med. 1976 September 1; 144(3): 768–775.
PMCID: PMC2190415

Fc receptor-mediated inhibition of murine B-lymphocyte activation

Abstract

Immobilized antigen-antibody complexes are able to inhibit the mitogenic response of murine spleen cells to the B-cell mitogen 8-bromo- 3',5'-cyclic guanosine monophosphoric acid. This this inhibition is dependent on intact Fc fragments in the immobilized complexes. Soluble complexes do not mediate this inhibition. When lipopolysaccharide (lps) activation of B cells was studied, it was found that the mitogenic response was inhibited at all times tested between 2 and 7 days of culture. Also, the LPS-induced mitogenesis of nude spleen cells was inhibited by immobilized complexes, indicating that suppressor T cells probably play no significant role in the inhibition. Immobilized complexes inhibit polyclonal antibody responses in a serum-free system and in the presence of normal mouse serum, but are unable to inhibit in the presence of fetal calf serum (FCS). If nu/nu spleen cells are used, however, the FCS does not block the ability of the complexes to inhibit the polyclonal response. It is suggested that that antigen-antibody complexes under appropriate conditions may bind to B lymphocytes via their Fc receptors and trigger a central "off" signal which blocks proliferation and consequently antibody production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anderson CL, Grey HM. Receptors for aggregated IgG on mouse lymphocytes: their presence on thymocytes, thymus-derived, and bone marrow-derived lymphocytes. J Exp Med. 1974 May 1;139(5):1175–1188. [PMC free article] [PubMed]
  • Nicholas R, Sinclair SC. Regulation of the immune response. I. Reduction in ability of specific antibody to inhibit long-lasting IgG immunological priming after removal of the Fc fragment. J Exp Med. 1969 Jun 1;129(6):1183–1201. [PMC free article] [PubMed]
  • Möller G, Coutinho A. Role of C'3 and Fc receptors in B-lymphocyte activation. J Exp Med. 1975 Mar 1;141(3):647–663. [PMC free article] [PubMed]
  • Ryan JL, Arbeit RD, Dickler HB, Henkart PA. Inhibition of lymphocyte mitogenesis by immobilized antigen-antibody complexes. J Exp Med. 1975 Oct 1;142(4):814–826. [PMC free article] [PubMed]
  • Mishell RI, Dutton RW. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. [PMC free article] [PubMed]
  • Weinstein Y, Segal S, Melmon KL. Specific mitogenic activity of 8-Br-guanosine 3',5'-monophosphate (Br-cyclic GMP) on B lymphocytes. J Immunol. 1975 Jul;115(1):112–117. [PubMed]
  • Stout RD, Herzenberg LA. The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor. J Exp Med. 1975 Nov 1;142(5):1041–1051. [PMC free article] [PubMed]
  • Dutton RW. Inhibitory and stimulatory effects of concanavalin A on the response of mouse spleen cell suspensions to antigen. II. Evidence for separate stimulatory and inhibitory cells. J Exp Med. 1973 Dec 1;138(6):1496–1505. [PMC free article] [PubMed]
  • Andersson J, Melchers F, Galanos C, Lüderitz O. The mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes. Lipid A as the mitogenic part of the molecule. J Exp Med. 1973 Apr 1;137(4):943–953. [PMC free article] [PubMed]
  • Watson J. The influence of intracellular levels of cyclic nucleotides on cell proliferation and the induction of antibody synthesis. J Exp Med. 1975 Jan 1;141(1):97–111. [PMC free article] [PubMed]
  • Shiigi SM, Mishell RI. Sera and the in vitro induction of immune responses. I. Bacterial contamination and the generation of good fetal bovine sera. J Immunol. 1975 Sep;115(3):741–744. [PubMed]
  • Kindred B. Antibody response in genetically thymus-less nude mice injected with normal thymus cells. J Immunol. 1971 Nov;107(5):1291–1295. [PubMed]
  • Kappler JW, van der Hoven A, Dharmarajan U, Hoffmann M. Regulation of the immune response. IV. Antibody-mediated suppression of the immune response to haptens and heterologous erythrocyte antigens in vitro. J Immunol. 1973 Oct;111(4):1228–1235. [PubMed]

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