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J Exp Med. 1989 October 1; 170(4): 1369–1385.
PMCID: PMC2189488

Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes


The structural heterogeneity of the human low affinity receptor for IgG, FcRII(CD32), has been elucidated through the isolation, characterization, and expression of cDNA clones derived from myeloid and lymphoid RNA. These clones predict amino acid sequences consistent with integral membrane glycoproteins with single membrane spanning domains. The extracellular domains display sequence homology to other Fc gamma Rs and members of the Ig supergene family. A minimum of three genes (Fc gamma RIIa, IIa', and Fc gamma RIIb) encode these transcripts, which demonstrate highly related extracellular and membrane spanning domains. IIa/IIa' differ substantially in the intracytoplasmic domain from IIb. Alternative splicing of the IIb gene generates further heterogeneity in both NH2- and COOH-terminal domains of the predicted proteins. Comparison to the murine homologues of these molecules reveals a high degree of conservation between the products of one of these genes, Fc gamma RIIb, and the murine beta gene in primary sequence, splicing pattern, and tissue distribution. In contrast, the sequence of IIa' indicates its relationship to the beta-like genes, with mutation giving rise to a novel cytoplasmic domain, while IIa is a chimera of both alpha- and beta-like genes. Expression of these cDNA molecules by transfection results in the appearance of IgG binding molecules that bear the epitopes defined by the FcRII(CD32) mAbs previously described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Nathan C, Cohn Z. Role of oxygen-dependent mechanisms in antibody-induced lysis of tumor cells by activated macrophages. J Exp Med. 1980 Jul 1;152(1):198–208. [PMC free article] [PubMed]
  • Bich-Thuy LT, Revillard JP. Selective suppression of human B lymphocyte differentiation into IgG-producing cells by soluble Fc gamma receptors. J Immunol. 1982 Jul;129(1):150–152. [PubMed]
  • Ravetch JV, Luster AD, Weinshank R, Kochan J, Pavlovec A, Portnoy DA, Hulmes J, Pan YC, Unkeless JC. Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors. Science. 1986 Nov 7;234(4777):718–725. [PubMed]
  • Lewis VA, Koch T, Plutner H, Mellman I. A complementary DNA clone for a macrophage-lymphocyte Fc receptor. Nature. 324(6095):372–375. [PubMed]
  • Hogarth PM, Hibbs ML, Bonadonna L, Scott BM, Witort E, Pietersz GA, McKenzie IF. The mouse Fc receptor for IgG (Ly-17): molecular cloning and specificity. Immunogenetics. 1987;26(3):161–168. [PubMed]
  • Weinshank RL, Luster AD, Ravetch JV. Function and regulation of a murine macrophage-specific IgG Fc receptor, Fc gamma R-alpha. J Exp Med. 1988 Jun 1;167(6):1909–1925. [PMC free article] [PubMed]
  • Perussia B, Tutt MM, Qiu WQ, Kuziel WA, Tucker PW, Trinchieri G, Bennett M, Ravetch JV, Kumar V. Murine natural killer cells express functional Fc gamma receptor II encoded by the Fc gamma R alpha gene. J Exp Med. 1989 Jul 1;170(1):73–86. [PMC free article] [PubMed]
  • Ravetch JV, Perussia B. Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J Exp Med. 1989 Aug 1;170(2):481–497. [PMC free article] [PubMed]
  • Selvaraj P, Rosse WF, Silber R, Springer TA. The major Fc receptor in blood has a phosphatidylinositol anchor and is deficient in paroxysmal nocturnal haemoglobinuria. Nature. 1988 Jun 9;333(6173):565–567. [PubMed]
  • Titus JA, Perez P, Kaubisch A, Garrido MA, Segal DM. Human K/natural killer cells targeted with hetero-cross-linked antibodies specifically lyse tumor cells in vitro and prevent tumor growth in vivo. J Immunol. 1987 Nov 1;139(9):3153–3158. [PubMed]
  • Lanier LL, Ruitenberg JJ, Phillips JH. Functional and biochemical analysis of CD16 antigen on natural killer cells and granulocytes. J Immunol. 1988 Nov 15;141(10):3478–3485. [PubMed]
  • Stuart SG, Trounstine ML, Vaux DJ, Koch T, Martens CL, Mellman I, Moore KW. Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII). J Exp Med. 1987 Dec 1;166(6):1668–1684. [PMC free article] [PubMed]
  • Hibbs ML, Bonadonna L, Scott BM, McKenzie IF, Hogarth PM. Molecular cloning of a human immunoglobulin G Fc receptor. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2240–2244. [PubMed]
  • Stengelin S, Stamenkovic I, Seed B. Isolation of cDNAs for two distinct human Fc receptors by ligand affinity cloning. EMBO J. 1988 Apr;7(4):1053–1059. [PubMed]
  • Bedwell DM, Strobel SA, Yun K, Jongeward GD, Emr SD. Sequence and structural requirements of a mitochondrial protein import signal defined by saturation cassette mutagenesis. Mol Cell Biol. 1989 Mar;9(3):1014–1025. [PMC free article] [PubMed]
  • Corbi AL, Miller LJ, O'Connor K, Larson RS, Springer TA. cDNA cloning and complete primary structure of the alpha subunit of a leukocyte adhesion glycoprotein, p150,95. EMBO J. 1987 Dec 20;6(13):4023–4028. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Looney RJ, Abraham GN, Anderson CL. Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J Immunol. 1986 Mar 1;136(5):1641–1647. [PubMed]
  • Vaughn M, Taylor M, Mohanakumar T. Characterization of human IgG Fc receptors. J Immunol. 1985 Dec;135(6):4059–4065. [PubMed]
  • Tepler I, Shimizu A, Leder P. The gene for the rat mast cell high affinity IgE receptor alpha chain. Structure and alternative mRNA splicing patterns. J Biol Chem. 1989 Apr 5;264(10):5912–5915. [PubMed]
  • Staden R. Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521–538. [PMC free article] [PubMed]

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