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J Exp Med. 1987 March 1; 165(3): 601–614.
PMCID: PMC2188281

The isolation and sequence of a novel gene from a human functional T cell line


Using a subtractive hybridization procedure we have constructed a cDNA library enriched for sequences present in functional human T cell lines, but not in human EBV-transformed B cell lines. We have isolated a cDNA clone, AH2-519, representing a novel gene, designated 519. This novel gene is expressed in functional human cytolytic and Th cell lines but not in a variety of other cell lines, including several long-term human T cell tumor lines. The expression of gene 519 is inducible in cultures of normal human PBL using antigenic or mitogenic stimulation. Neither the DNA sequence determined from a full-length cDNA clone overlapping with clone AH2-519 nor the amino acid sequence of its predicted protein product has significant homology to published sequences in the GenBank or NBRF databases. The restricted expression of gene 519 suggests that its gene product is involved in the growth and/or differentiation of normal T cells. The data also show that normal, nontransformed, functional T cells express gene products that can not be readily identified in long-term tumor lines of the same cell lineage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Gillis S, Smith KA. Long term culture of tumour-specific cytotoxic T cells. Nature. 1977 Jul 14;268(5616):154–156. [PubMed]
  • Burakoff SJ, Weinberger O, Krensky AM, Reiss CS. A molecular analysis of the cytolytic T lymphocyte response. Adv Immunol. 1984;36:45–85. [PubMed]
  • Krensky AM, Reiss CS, Mier JW, Strominger JL, Burakoff SJ. Long-term human cytolytic T-cell lines allospecific for HLA-DR6 antigen are OKT4+. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2365–2369. [PubMed]
  • Krensky AM, Clayberger C, Reiss CS, Strominger JL, Burakoff SJ. Specificity of OKT4+ cytotoxic T lymphocyte clones. J Immunol. 1982 Nov;129(5):2001–2003. [PubMed]
  • Davis MM, Cohen DI, Nielsen EA, Steinmetz M, Paul WE, Hood L. Cell-type-specific cDNA probes and the murine I region: the localization and orientation of Ad alpha. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2194–2198. [PubMed]
  • Aviv H, Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. [PubMed]
  • Gubler U, Hoffman BJ. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. [PubMed]
  • Krieg PA, Melton DA. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 1984 Sep 25;12(18):7057–7070. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Smith KA. Interleukin 2. Annu Rev Immunol. 1984;2:319–333. [PubMed]
  • Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. [PMC free article] [PubMed]
  • von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. [PubMed]
  • Kahana C, Nathans D. Nucleotide sequence of murine ornithine decarboxylase mRNA. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1673–1677. [PubMed]
  • Thireos G, Penn MD, Greer H. 5' untranslated sequences are required for the translational control of a yeast regulatory gene. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5096–5100. [PubMed]
  • Efrat S, Pilo S, Kaempfer R. Kinetics of induction and molecular size of mRNAs encoding human interleukin-2 and gamma-interferon. Nature. 1982 May 20;297(5863):236–239. [PubMed]
  • Fujita T, Shibuya H, Ohashi T, Yamanishi K, Taniguchi T. Regulation of human interleukin-2 gene: functional DNA sequences in the 5' flanking region for the gene expression in activated T lymphocytes. Cell. 1986 Aug 1;46(3):401–405. [PubMed]
  • Krönke M, Leonard WJ, Depper JM, Greene WC. Sequential expression of genes involved in human T lymphocyte growth and differentiation. J Exp Med. 1985 Jun 1;161(6):1593–1598. [PMC free article] [PubMed]
  • Greene WC, Leonard WJ. The human interleukin-2 receptor. Annu Rev Immunol. 1986;4:69–95. [PubMed]
  • Gershenfeld HK, Weissman IL. Cloning of a cDNA for a T cell-specific serine protease from a cytotoxic T lymphocyte. Science. 1986 May 16;232(4752):854–858. [PubMed]
  • Lobe CG, Finlay BB, Paranchych W, Paetkau VH, Bleackley RC. Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes. Science. 1986 May 16;232(4752):858–861. [PubMed]
  • Brunet JF, Dosseto M, Denizot F, Mattei MG, Clark WR, Haqqi TM, Ferrier P, Nabholz M, Schmitt-Verhulst AM, Luciani MF, et al. The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14. Nature. 1986 Jul 17;322(6076):268–271. [PubMed]
  • Krensky AM, Clayberger C. Diagnostic and therapeutic implications of T cell surface antigens. Transplantation. 1985 Apr;39(4):339–348. [PubMed]
  • Suciu-Foca N, Reed E, Rubinstein P, MacKenzie W, Ng AK, King DW. A late-differentiation antigen associated with the helper inducer function of human T cells. Nature. 1985 Dec 5;318(6045):465–467. [PubMed]
  • Hemler ME, Sanchez-Madrid F, Flotte TJ, Krensky AM, Burakoff SJ, Bhan AK, Springer TA, Strominger JL. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J Immunol. 1984 Jun;132(6):3011–3018. [PubMed]

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