PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jexpmedHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
 
J Exp Med. 1986 June 1; 163(6): 1405–1414.
PMCID: PMC2188115

Purification to homogeneity of B cell stimulating factor. A molecule that stimulates proliferation of multiple lymphokine-dependent cell lines

Abstract

Murine B cell stimulating factor 1 (BSF-1) was purified to homogeneity from supernatants of a stimulated thymoma cell line. A protein of 18.4 kD with a unique N-terminal amino acid sequence was identified. BSF-1 had a sp act of at least 3.28 X 10(8) U/mg. In addition to its B cell- stimulatory activity, BSF-1 also stimulated the proliferation of several IL-2- and IL-3-dependent cell lines. We conclude that BSF-1 is both a growth factor and a differentiation factor. Finally, these results also suggest additional biologic properties of BSF-1 on lineages besides B lymphocytes.

Full Text

The Full Text of this article is available as a PDF (651K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Mond JJ, Thompson C, Finkelman FD, Farrar J, Schaefer M, Robb RJ. Affinity-purified interleukin 2 induces proliferation of large but not small B cells. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1518–1521. [PubMed]
  • Zubler RH, Lowenthal JW, Erard F, Hashimoto N, Devos R, MacDonald HR. Activated B cells express receptors for, and proliferate in response to, pure interleukin 2. J Exp Med. 1984 Oct 1;160(4):1170–1183. [PMC free article] [PubMed]
  • Sidman CL, Marshall JD, Shultz LD, Gray PW, Johnson HM. Gamma-interferon is one of several direct B cell-maturing lymphokines. Nature. 309(5971):801–804. [PubMed]
  • Leibson HJ, Gefter M, Zlotnik A, Marrack P, Kappler JW. Role of gamma-interferon in antibody-producing responses. Nature. 309(5971):799–801. [PubMed]
  • Howard M, Farrar J, Hilfiker M, Johnson B, Takatsu K, Hamaoka T, Paul WE. Identification of a T cell-derived b cell growth factor distinct from interleukin 2. J Exp Med. 1982 Mar 1;155(3):914–923. [PMC free article] [PubMed]
  • Farrar JJ, Howard M, Fuller-Farrar J, Paul WE. Biochemical and physicochemical characterization of mouse B cell growth factor: a lymphokine distinct from interleukin 2. J Immunol. 1983 Oct;131(4):1838–1842. [PubMed]
  • Dutton RW, Wetzel GD, Swain SL. Partial purification and characterization of a BCGFII from EL4 culture supernatants. J Immunol. 1984 May;132(5):2451–2456. [PubMed]
  • Rabin EM, Ohara J, Paul WE. B-cell stimulatory factor 1 activates resting B cells. Proc Natl Acad Sci U S A. 1985 May;82(9):2935–2939. [PubMed]
  • Oliver K, Noelle RJ, Uhr JW, Krammer PH, Vitetta ES. B-cell growth factor (B-cell growth factor I or B-cell-stimulating factor, provisional 1) is a differentiation factor for resting B cells and may not induce cell growth. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2465–2467. [PubMed]
  • Le Gros GS, Prestidge RL, Watson JD. In-vivo modulation of thymus-derived lymphocytes with monoclonal antibodies in mice. I. Effect of anti-Thy-1 antibody on the tissue distribution of lymphocytes. Immunology. 1983 Dec;50(4):537–546. [PubMed]
  • Gillis S, Smith KA. Long term culture of tumour-specific cytotoxic T cells. Nature. 1977 Jul 14;268(5616):154–156. [PubMed]
  • Dexter TM, Garland J, Scott D, Scolnick E, Metcalf D. Growth of factor-dependent hemopoietic precursor cell lines. J Exp Med. 1980 Oct 1;152(4):1036–1047. [PMC free article] [PubMed]
  • Greenberger JS, Eckner RJ, Sakakeeny M, Marks P, Reid D, Nabel G, Hapel A, Ihle JN, Humphries KC. Interleukin 3-dependent hematopoietic progenitor cell lines. Fed Proc. 1983 Jul;42(10):2762–2771. [PubMed]
  • Brake AJ, Merryweather JP, Coit DG, Heberlein UA, Masiarz FR, Mullenbach GT, Urdea MS, Valenzuela P, Barr PJ. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4642–4646. [PubMed]
  • Stern AS, Pan YC, Urdal DL, Mochizuki DY, DeChiara S, Blacher R, Wideman J, Gillis S. Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia. Proc Natl Acad Sci U S A. 1984 Feb;81(3):871–875. [PubMed]
  • Urdal DL, Mochizuki D, Conlon PJ, March CJ, Remerowski ML, Eisenman J, Ramthun C, Gillis S. Lymphokine purification by reversed-phase high-performance liquid chromatography. J Chromatogr. 1984 Jul 27;296:171–179. [PubMed]
  • Ohara J, Lahet S, Inman J, Paul WE. Partial purification of murine B cell stimulatory factor (BSF)-1. J Immunol. 1985 Oct;135(4):2518–2523. [PubMed]
  • Kronheim SR, March CJ, Erb SK, Conlon PJ, Mochizuki DY, Hopp TP. Human interleukin 1. Purification to homogeneity. J Exp Med. 1985 Mar 1;161(3):490–502. [PMC free article] [PubMed]
  • Dayhoff MO, Barker WC, Hunt LT. Establishing homologies in protein sequences. Methods Enzymol. 1983;91:524–545. [PubMed]
  • Noelle R, Krammer PH, Ohara J, Uhr JW, Vitetta ES. Increased expression of Ia antigens on resting B cells: an additional role for B-cell growth factor. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6149–6153. [PubMed]
  • Roehm NW, Leibson HJ, Zlotnik A, Kappler J, Marrack P, Cambier JC. Interleukin-induced increase in Ia expression by normal mouse B cells. J Exp Med. 1984 Sep 1;160(3):679–694. [PMC free article] [PubMed]
  • Vitetta ES, Ohara J, Myers CD, Layton JE, Krammer PH, Paul WE. Serological, biochemical, and functional identity of B cell-stimulatory factor 1 and B cell differentiation factor for IgG1. J Exp Med. 1985 Nov 1;162(5):1726–1731. [PMC free article] [PubMed]
  • Yuan D, Weiss EA, Layton JE, Krammer PH, Vitetta ES. Activation of the gamma 1 gene by lipopolysaccharide and T cell-derived lymphokines containing a B cell differentiation factor for IgG1 (BCDF gamma). J Immunol. 1985 Aug;135(2):1465–1469. [PubMed]
  • Jones S, Layton J, Krammer PH, Vitetta ES, Tucker PW. The effect of T cell-derived lymphokines on the levels of isotype-specific RNA in normal B cells. J Mol Cell Immunol. 1985;2(3):143–153. [PubMed]
  • Reem GH, Yeh NH. Interleukin 2 regulates expression of its receptor and synthesis of gamma interferon by human T lymphocytes. Science. 1984 Jul 27;225(4660):429–430. [PubMed]
  • Farrar WL, Johnson HM, Farrar JJ. Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2. J Immunol. 1981 Mar;126(3):1120–1125. [PubMed]
  • Welte K, Andreeff M, Platzer E, Holloway K, Rubin BY, Moore MA, Mertelsmann R. Interleukin 2 regulates the expression of Tac antigen on peripheral blood T lymphocytes. J Exp Med. 1984 Nov 1;160(5):1390–1403. [PMC free article] [PubMed]
  • Grabstein KH, Urdal DL, Tushinski RJ, Mochizuki DY, Price VL, Cantrell MA, Gillis S, Conlon PJ. Induction of macrophage tumoricidal activity by granulocyte-macrophage colony-stimulating factor. Science. 1986 Apr 25;232(4749):506–508. [PubMed]
  • Lopez AF, Nicola NA, Burgess AW, Metcalf D, Battye FL, Sewell WA, Vadas M. Activation of granulocyte cytotoxic function by purified mouse colony-stimulating factors. J Immunol. 1983 Dec;131(6):2983–2988. [PubMed]
  • Koyasu S, Yodoi J, Nikaido T, Tagaya Y, Taniguchi Y, Honjo T, Yahara I. Expression of interleukin 2 receptors on interleukin 3-dependent cell lines. J Immunol. 1986 Feb 1;136(3):984–987. [PubMed]
  • Le Gros GS, Gillis S, Watson JD. Induction of IL 2 responsiveness in a murine IL 3-dependent cell line. J Immunol. 1985 Dec;135(6):4009–4014. [PubMed]
  • Sanderson CJ, O'Garra A, Warren DJ, Klaus GG. Eosinophil differentiation factor also has B-cell growth factor activity: proposed name interleukin 4. Proc Natl Acad Sci U S A. 1986 Jan;83(2):437–440. [PubMed]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press