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Cell matrix adhesion is required for cell proliferation and survival. Here we report that mutation by gene targeting of the cytoplasmic tail of β1 integrin leads to defective proliferation and survival both in vivo and in vitro. Primary murine embryonic fibroblasts (MEFs) derived from mutant homozygotes display defective cell cycle coupled to impaired activation of the FAK-PI3K-Akt and Rac-JNK signaling pathways. Expression in homozygous MEFs of a constitutively active form of Rac is able to rescue proliferation, survival, and JNK activation. Moreover, although showing normal Erk phosphorylation, mutant cells fail to display Erk nuclear translocation upon fibronectin adhesion. However, expression of the constitutively activated form of Rac restores Erk nuclear localization, suggesting that adhesion-dependent Rac activation is necessary to integrate signals directed to promote MAPK activity. Altogether, our data provide the evidence for an epistatic interaction between the β1 integrin cytoplasmic domain and Rac, and indicate that this anchorage-dependent signaling pathway is crucial for cell growth control.
Modulation of cell adhesion is known to influence essential cellular processes such as proliferation, differentiation, and migration. Major molecular mediators of these effects are integrins, heterodimeric transmembrane glycoproteins able to link extracellular matrix components and intracellular actin cytoskeleton. Integrins are formed by α and β subunits which act together (Hynes, 1992). So far, 8 β and 18 α subunits have been described, and the β1 subunit is contained in 11 receptors with distinct α chains. The β1 subunit is also present in alternatively spliced isoforms of the cytoplasmic C-terminal tail. Murine cells express a ubiquitous A and a muscle-specific D isoform (Belkin et al., 1996).
By binding to a large variety of interactors (Hemler, 1998; Liu et al., 2000), the short cytoplasmic sequence of all β1 variants regulates several kind of transmembrane signaling events (Sastry and Horwitz, 1993). The most membrane proximal segment is encoded by a single exon (exon 6) that is shared by all β1 splice variants; it spans 25 amino acids able to interact with focal adhesion kinase (FAK),* paxillin, α-actinin, and melusin (Otey et al., 1993; Schaller et al., 1995; Brancaccio et al., 1999). On the other hand, each splice variant is characterized by the presence of distinct C-terminal ends. Amino acids of the β1A-specific tail bind cytoskeletal components like talin and filamin (Pfaff et al., 1998) or signaling effectors like ICAP1 (Chang et al., 1997; Degani et al., 2002) and ILK (Dedhar and Hannigan, 1996) (Fig. 1).
The wide variety of cell adhesion events mediated by β1 integrins is crucial in vivo and essential for mammalian development (Fässler et al., 1996). Studies of anchorage-dependent cell growth suggest that integrins, together with growth factor receptors, activate mitogen-activated protein kinases and thus allow cell cycle progression. Adhesion to the extracellular matrix leads to the α integrin subunit-dependent activation of Fyn, and to the subsequent recruitment of Shc (Giancotti and Ruoslahti, 1999). In addition, the β integrin subunit is involved in the activation of the tyrosine kinase FAK. Both pathways are able to link adhesive events to the phosphorylation of Erk by activating Ras via direct binding of either FAK or Shc to Grb2 (Schlaepfer et al., 1994; Wary et al., 1996). Recently, it has been shown that FAK can also activate Erk through a mechanism involving B-Raf and Rap1 (Barberis et al., 2000). Although concerted Erk activation by adhesion and growth factors is considered necessary for Cyclin D1 induction (Roovers et al., 1999), some reports indicate that other adhesion-mediated signals are required for cell cycle progression (Le Gall et al., 1998). For example, in parallel to Erk activation, integrins trigger signals that allow the coupling of the Rac GTPase to its downstream effectors (del Pozo et al., 2000; Mettouchi et al., 2001).
Using a gene-targeting strategy in embryonic stem (ES) cells, we have isolated a β1 integrin allele encoding a mutant cytoplasmic domain (β1de6 allele) in which the alternatively spliced C-terminal sequence was substituted with 11 amino acids unrelated to all known β1 splice variants. Homozygous mice die between embryonic day 10.5 and 11.5. Moreover, mutant embryos show impaired cell proliferation and survival. Primary murine embryonic fibroblasts (MEFs) derived from mutant homozygotes display normal adhesion to fibronectin (Barberis et al., 2000) but exhibit strongly impaired integrin-mediated activation of PI3K, Rac, and JNK. In addition, mutant MEFs, although presenting normally phosphorylated Erk (Barberis et al., 2000), fail to translocate Erk into the nucleus. Erk nuclear localization, JNK activation, and proliferation and survival are rescued by a constitutively active form of Rac, showing that β1 integrin–induced Rac activation is a crucial step for anchorage-dependent control of cell growth.
A β1 integrin cytoplasmic domain mutant allele (β1de6) was obtained by gene targeting in murine ES cells. This allele was found in a single targeted clone, and consequently to a unique recombination event carried the duplication of exon 6 as indicated in Fig. 2. This structure lead to the expression of a β1 subunit in which the cytoplasmic sequence shared by all β1 integrin splice variants was followed by an irrelevant C-terminal tail sequence, consisting of 11 amino acids (with a predicted sequence: TVLLVPTSSQL) unrelated to all known β1 integrin isoforms.
ES cells deriving from the targeted clone carrying the β1de6 allele were injected into C57B6 host blastocysts, and germ line transmitting chimeras were generated. Heterozygous animals were viable and fertile. Histology of various tissues from these animals did not show any abnormality. Genotyping of litters derived from heterozygotes crosses showed that among 672 pups, 448 were heterozygous and 224 were wild-type homozygous. These results were consistent with a recessive embryonic lethal phenotype caused by homozygosity for the β1de6 allele.
Embryos derived from heterozygous intercrosses were dissected and genotyped by PCR. Homozygous mutants that were exteriorly indistinguishable from wild-type littermates could be identified until 8.5 d postcoitum (dpc). Later, β1de6/β1de6 embryos started to exhibit retarded growth and a plethora of variably combined abnormalities. At 9.5 dpc, 50% of the mutant homozygous embryos showed the failure of the allantois to fuse with the chorion (Fig. 3, A and B). Histology of 9.5-dpc β1de6 homozygotes indicated that when the allantois was not fused to the chorion, embryos appeared abnormally oriented in the decidua (Fig. 3, C–E). At 10.5 dpc, 95% of mutants displayed cardiac and/or craniofacial abnormalities. Heart disfunction was suggested by the presence of pericardiac effusion (Fig. 3, F and G). The severity of this phenotype was variable and ranged from mild liquid accumulation in the pericardial cavity to severe developmental retardation and pericardial swelling. In addition, mutants showed exencephaly (20% penetrance; Fig. 3 H) and/or abnormally waved or kinky neural tube (90% penetrance; Fig. 3 J). Moreover, mutant embryos exhibited blood leakage in the yolk sac, delayed somite development, and decreased density of mesenchymal cells (Fig. 3 J). At 11.5 dpc, mutant embryos showed no heart beating, appeared much smaller than wild-type littermates, and were already in process of being resorbed. At 12.5 dpc, no β1de6 homozygotes could be detected, and ~1/4 of the implantation sites contained only blood. Identical results were obtained from the analysis of both C57/129Sv mixed genotype and 129Sv inbred intercrosses.
To analyze whether β1de6 homozygous embryos were still able to express β1 integrin variants, RT-PCR analysis was performed to detect the presence of the β1A and β1D transcripts. Total RNA was extracted from mutant and wild-type embryos (9.5 dpc) and, as shown in Fig. 4 A, β1A and β1D could be amplified from wild-type RNA but not from β1de6 homozygous samples.
The expression of the mutant protein was analyzed by establishing cultures of MEFs isolated from 9.5 dpc embryos. To test the expression of the mutant integrin, the surface of wild-type and mutant MEFs was biotinylated, and extracts were immunoprecipitated using a polyclonal antiserum that recognized the β1A-specific sequence. As indicated in Fig. 4 B (left), β1A could be immunoprecipitated uniquely from wild-type cells. In contrast, a β1 integrin extracellular epitope could be detected in MEFs of both genotypes (Fig. 4 B, right). These data indicate that β1de6 homozygous cells expressed a single β1 integrin isoform carrying a mutant cytoplasmic domain. The alteration of the β1 C-terminal tail was previously associated with defective recruitment of the protein into focal contacts. Nevertheless, in agreement with a crucial role of α subunits for integrin targeting to focal adhesions, immunofluorescence with anti-vinculin and anti-β1 antibodies showed that, in mutant MEFs plated on fibronectin, β1de6 distributed in focal contacts as efficiently as wild-type β1 (Fig. 4, C–F).
Altogether, these data demonstrate that β1de6 is the only β1 integrin isoform expressed by homozygous mutant MEFs and that the altered cytoplasmic domain does not interfere with its correct cellular distribution.
Integrin-mediated signaling is thought to control anchorage-dependent cell growth; thus, it is possible that β1de6 affects cell proliferation and survival. To test this hypothesis in vivo without being biased by a general defective growth due to abnormal materno-fetal circulation, 8.5-d-old embryos showing heart beating and normal placentation were studied. Counts of BrdU-labeled nuclei in wild-type and mutant embryos showed a small but significant 10% decrease of proliferating cells in β1de6 homozygotes (wild-type tissue: 82 ± 6 BrdU-labeled nuclei/total nuclei per field *100, n = 574; mutant tissue: 74 ± 6, n = 591 – values expressed as mean ± SD; P = 0.01 by Student's t test). Strikingly, older embryos (9.5 dpc) showed a higher reduction of BrdU staining which was particularly evident in the mesenchyme (Fig. 5, A and B), where mutant cells exhibited a 30% decrease in the number of labeled nuclei (wild-type tissue: 47 ± 10 BrdU labeled nuclei/total nuclei per field *100, n = 8213; mutant tissue: 32 ± 9, n = 7433 – values expressed as mean ± SD; P = 0.03 by Student's t test). In addition, detection by TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay of apoptotic cells in sections of 9.5 dpc embryos revealed that mutant homozygotes also showed significantly higher numbers of TUNEL-positive cells in the mesenchyme and heart (Fig. 5, C and D).
Consistent with these findings, β1de6 homozygous MEFs showed a modified cell cycle profile. Whereas analysis of DNA content by FACS of synchronized mutant cells displayed a modest but statistically significant increase of cells in G1 (25% increment), the number of cells in S phase was lowered by 30% and cells in G2 were strongly reduced (76% decrease; Fig. 5 E). Moreover, testing of MEFs for adhesion-dependent cell proliferation indicated reduced numbers of BrdU labeled cells in cultures of mutant cells (Fig. 5 F). Interestingly, this proliferation defect was maximal on the β1 integrin ligand fibronectin but not on the β3 ligand vitronectin, thus showing the specificity of the defect (Fig. 5 F). Altogether, these data suggested that β1de6 homozygous MEFs were characterized by an impaired or delayed G1/S transition. In agreement with this hypothesis, synchronized cultures showed that clearance of the cyclin dependent kinase inhibitor p27kip1 together with accumulation of cyclin D1 occurred more slowly in mutant cells than in wild-type controls (Fig. 5 G).
In addition, in vitro analysis revealed in mutant MEFs reduced adhesion-mediated cell survival (Fig. 5 H). Consistent with these results, detection of caspase-3 activation in vivo (Fig. 5 I) exhibited, in β1de6 homozygous embryos, a significant decrease of inactive procaspase-3 and a concomitant increment of cleaved active caspase-3. These results demonstrate that the expression of β1de6 mutant integrin likely impairs anchorage-dependent signaling events that sustain cell proliferation and survival.
Previous results revealed that β1de6 homozygous MEFs normally adhere to fibronectin but are not able to activate the tyrosine kinase FAK (Barberis et al., 2000). Because FAK activity has been shown to regulate PI3K, a molecule involved in controlling proliferation and survival, we investigated whether the presence of β1de6 could interfere with adhesion-dependent PI3K activation. First, the ability of p85 PI3K regulatory subunit to coimmunoprecipitate with FAK was tested after adhesion to fibronectin. As shown in Fig. 6 A, recruitment of p85 to a FAK-containing complex was strongly reduced in β1de6/β1de6 cells.
Next, PI3K activity induced by adhesion to fibronectin was tested by analyzing phosphorylation of the PI3K-dependent downstream target PKB/Akt. As shown in Fig. 6 C, whereas in wild-type cells PKB/Akt was activated by the adhesive stimulus, in β1de6 homozygous cells PKB/Akt phosphorylation was undetectable. Because activation of PI3K is crucial for PKB/Akt phosphorylation, these data strongly suggest that the β1 integrin cytoplasmic domain plays a crucial role in FAK-dependent PI3K activation.
In fibroblasts, integrins contribute to the activation of the small GTPase Rac by triggering multiple signaling pathways, such as p130Cas/Crk/DOCK180 (Kiyokawa et al., 1998), PI3K/SOS (Mettouchi et al., 2001), and possibly paxillin activation (Igishi et al., 1999). Interestingly, analysis of adhesion-stimulated cells showed that paxillin phosphosphorylation is delayed and reduced in β1de6 homozygous MEFs (Fig. 6 D). Together with this observation, the lack of adhesion-stimulated activation of PI3K along with the previous observation that signaling through p130Cas is blocked (Barberis et al., 2000) suggested that, in mutant cells, Rac activation could be impaired. Indeed, pulldown assays using the CRIB domain of PAK-1 fused to GST revealed that, after plating β1de6 homozygous MEFs on fibronectin, Rac activation was heavily impaired both in the absence (Fig. 7 A) and in the presence (Fig. 7 B) of growth factors. Accordingly to this result, mutant cells analyzed by immunofluorescence at 30 min after fibronectin adhesion in the presence of growth factors showed decreased lamellipodia formation (Fig. 7 C).
Downstream, Rac stimulates the activation of JNK, a stress-activated protein kinase (SAPK) known to control cell proliferation and survival (Leppa and Bohmann, 1999). Activation of JNK and of another SAPK, p38, was thus examined in β1de6 homozygous MEFs. As shown in Fig. 7, D and E, plating on fibronectin stimulates the phosphorylation of both JNK and p38. In contrast, these activation events were undetectable in the mutant cells.
These data point to a crucial role for the C-terminal tail of β1 integrin in the activation of Rac and the subsequent phosphorylation of JNK.
To investigate whether impaired proliferation and survival in β1de6 homozygous embryos were caused by defective Rac activation, MEFs were infected with adenoviruses expressing a constitutively active (RacV12) or a dominant negative (RacN17) form of Rac.
After expression of RacV12, β1de6 homozygous MEFs were able to activate JNK as efficiently as wild-type cells. Interestingly, RacV12 expression led to adhesion-independent JNK activation: similar JNK phosphorylation could be detected in both wild-type and mutant infected fibroblasts kept in suspension. Conversely, wild-type fibroblasts expressing RacN17 showed a strong reduction in activated JNK when adhering to fibronectin (Fig. 8 B).
Next, proliferation and survival were tested by BrdU and TUNEL assays in synchronized cell cultures of wild-type and mutant MEFs expressing RacV12. As shown in Fig. 8, C and D, the proliferation defect and the increased apoptosis of mutant cells were completely rescued. On the other hand, the presence of RacN17 in wild-type MEFs was sufficient to cause the increased apoptosis and the reduced cell growth exhibited by untreated mutant MEFs.
In addition to JNK, Erk 1 and 2 play a crucial role in transducing mitogenic signals. MEFs homozygous for the β1de6 allele normally phosphorylate Erk in response to a combined stimulation by adhesion and growth factors (Barberis et al., 2000); however, in a large majority of these cells (85%; P << 0.001 by χ2 test versus wild-type controls; n = 300 for each genotype) Erk nuclear translocation was strongly impaired (Fig. 9). Accordingly, forced Erk phosphorylation in suspended cells is not sufficient for nuclear translocation and activation of the transcription factor Elk (Aplin et al., 2001). To investigate the nature of the integrin-dependent signal required for Erk nuclear localization, we next examined whether Rac played a role in this process. Strikingly, expression of RacV12 in β1de6 homozygous cells, adhering to fibronectin in the presence of growth factors, caused a strong enrichment of nuclear Erk; on the contrary, expression of RacN17 in wild-type cells decreased amounts of nuclear Erk (Fig. 9). Thus, integrin-dependent Rac activation is a critical step for Erk subcellular localization.
In conclusion, the findings presented here show an epistatic interaction between the β1 integrin cytoplasmic domain and Rac and demonstrate that this signaling pathway, by integrating JNK and Erk activation, plays an essential role in anchorage-dependent cell cycle progression, as well as cell survival.
Our study demonstrates that, in β1de6 homozygous mice, mutation of the C-terminal tail of the β1 integrin subunit causes in vivo and in vitro defective adhesion-dependent cell proliferation and survival and ultimately a recessive embryonic lethal phenotype.
Homozygous β1de6 embryos developed to midgestation but then died showing multiple variably penetrant phenotypes. Although these phenotypes could be analyzed in mice derived from a single ES clone, the chance that they were caused by a clonal artifact is very unlikely. In fact, they appeared stable and reproducible >30 generations in two distinct genetic backgrounds (inbred 129Sv and mixed 129Sv/C57B6), as well as after 10 generations of backcrossing with C57B6 mice. This stability, after such a dilution of the original ES clone genotype, supports the direct involvement of the β1de6 mutation in the reported phenotypes.
Interestingly, β1de6 homozygous embryos were found to survive longer than β1-null mutants which die around embryonic day 5 (Fässler et al., 1996). Because the β1 integrin cytoplasmic domain consists of a C-terminal tail that is subjected to alternative splicing and of a common juxtamembrane tract that is present in β1de6 and all β1 splice variants, our data suggest that the alternatively spliced region of the β1 cytoplasmic domain is dispensable in early morphogenetic processes. Likewise, our results indicate that the membrane proximal subdomain still present in the β1de6 integrin is sufficient to generate the signals and/or cytoskeletal linkage required for these events. Consistently, mice ectopically expressing β1D, a muscle-specific splicing variant which also contains the wild-type common cytodomain, survive beyond implantation but die before birth because of variably penetrant phenotypes that, like in β1de6 homozygotes, include vascular and neuroepithelial malformations (Baudoin et al., 1998).
The presence in β1de6 homozygous embryos of vascular abnormalities and defective placentation suggests that this might be the reason for the lethality. Similar defects are the major causes of the death of mutant embryos lacking distinct integrin α subunits like α4 and α5 (for review see Brakebusch et al., 1997). For example, the aberrant mesenchyme development (Fig. 3 J) and the leaking blood islands (Fig. 3, G and J) seen in β1de6 homozygotes have been similarly detected in α5-deficient embryos (Yang et al., 1993). However, the overall phenotype of β1de6 homozygotes, although sharing some characteristics of the α5-null embryos, strongly overlaps with that of α4-null mutants. In fact, like β1de6/β1de6 embryos, mice lacking the α4 integrin subunit die at embryonic day 11, showing variably penetrant phenotypes such as heart abnormalities and absence of chorion-allantois fusion (Yang et al., 1995). Although the explanation for this correspondence is not clear, on the basis that distinct α integrin subunits show distinct biological properties, bind different intracellular proteins (Hemler, 1998; Liu et al., 2000) and specifically cooperate with the β1 cytodomain for signaling (Sastry et al., 1999), it is tempting to speculate that signaling of α4 more strongly requires the β1 C-terminal tail than that of α5.
Despite the similarity between β1de6/ β1de6 and α4-null embryos, our analysis indicates that in vivo expression of β1de6 leads to defective cell proliferation and survival independently of the vascular phenotypes. In fact, decreasing numbers of dividing cells and increased apoptosis could be detected in β1de6 homozygous embryos that showed normal placentation and still had an apparently functional heart.
On the basis of in vitro experiments, it has been suggested that integrins may play a major role in allowing the G1-phase cell cycle progression (Assoian, 1997). The involvement of integrins in permitting S phase entry has been highlighted by the requirement of adhesion and spreading onto extracellular matrix for triggering cyclin D1 accumulation and p27kip1 decrease (Assoian and Schwartz, 2001). Furthermore, binding to distinct integrin ligands can selectively inhibit or promote cell growth (Giancotti, 2000). Nevertheless, extracellular matrix adhesion is a complex process in which several receptors other than integrins may contribute. The finding of signaling properties for transmembrane molecules like CD44 and syndecans (Couchman and Woods, 1999) complicates the assignment to integrins of cell matrix adhesion-triggered signals. Our results in vivo and in primary cell cultures clearly demonstrated that signaling from the β1 integrin cytodomain plays a central role in anchorage-dependent cell growth. Consistent with a role of β1 integrin cytodomain in these processes, inhibitory effects on cell proliferation can be detected after transfection of immortalized cells with the β1C (Fornaro et al, 1995; Meredith et al., 1995) or the β1D (Belkin and Retta, 1998) splicing variants.
A requirement for integrin-mediated signal transduction is the clustering of these molecules into discrete subcellular domains like focal contacts. The finding that β1de6 integrins localizes into these districts was unexpected, as other β1 integrin C-terminal tail mutants have been reported to fail to enter focal adhesions. Nevertheless, similarly to β1de6, the β1B cytoplasmic domain variant can localize under specific conditions to a subset of focal contacts (Armulik et al., 2000). Because studies of β1 integrin cytodomain mutants have been carried out by transfection of transformed cell lines, the distribution of β1de6 might be due to a specific behavior of primary MEFs. Alternatively, it is conceivable that specific adaptations allowing homozygous mutant cells to localize β1de6 in focal contacts were selected during ontogeny. However, the recruitment of β1de6 in focal adhesions excludes the possibility that the signaling defects observed in β1de6 homozygous MEFs were caused by a displacement from the correct intracellular location. It is interesting to note that mutant MEFs used for localization experiments after adhering to fibronectin for 2 h appeared as spread as wild-type cells. This is in apparent contrast with the impaired Rac activation detected in β1de6 homozygous fibroblasts. However, the immunofluorescence assays were performed in the presence of growth factors, a condition where Rac activation in mutant MEFs is decreased but not abolished. Therefore, it is possible that this residual Rac activity is sufficient to support cell spreading. Nonetheless, consistently with a deficient Rac activation, mutant cell showed a decreased spreading rate (Barberis et al., 2000) and displayed a reduced number of lamellipodia after 30 min of fibronectin adhesion.
Analysis of signaling properties of β1de6 homozygous MEFs showed defective adhesion-triggered FAK, paxillin, PI3K, Rac, and JNK activation. Chen et al. (1996) have shown that FAK phosphorylation causes PI3K and PKB/Akt activation. This signaling pathway has been correlated to the protection from apoptosis induced by detachment from the extracellular matrix (anoikis) (Frisch and Ruoslahti, 1997). Because activated PKB/Akt is known to regulate apoptosis by phosphorylating pro-apoptotic molecules such as BAD and caspase 9 (Datta et al., 1999), the increased apoptosis in β1de6/β1de6 MEFs might depend on the defective activation of PI3K/PKB/Akt.
Recently, PI3K has been found to play an essential role in adhesion-dependent Rac activation in endothelial cells (Mettouchi et al., 2001). This process is mediated by the guanine nucleotide exchange factor SOS that, in the presence of PI3K products, becomes an activator of Rac. However, it has been reported that in fibroblasts, a second pathway, requiring interaction of p130Cas, Crk, and DOCK180 plays a prominent role in Rac activation. Because p130Cas is not activated in β1de6 homozygous MEFs (Barberis et al., 2000), we could not define the respective contribution of these two pathways. Nevertheless, the signaling pathway triggered by p130Cas has central role in promoting matrix-dependent survival. Adhesion-dependent p130Cas phosphorylation and recruitment of Crk trigger antiapoptotic signals which are relayed by the subsequent activation of Rac and Jnk (Dolfi et al., 1998). In addition, Almeida et al. (2000) reported that the Cas-Rac-PAK-MKK4-JNK route is activated by fibronectin adhesion and promotes survival of primary fibroblasts. In agreement, the finding that Rac rescues β1de6 homozygous MEFs from programmed cell death, further supports the notion that the FAK-Cas-Rac-JNK pathway plays a fundamental role in preventing anoikis.
Interestingly, although FAK is not activated in β1de6/β1de6 MEFS, the lack of FAK in mice results in an embryonic lethal phenotype that is much more severe than that of β1de6 homozygotes. FAK-null embryos do not develop beyond 8 dpc and show strong deficits in mesenchymal development apparently linked to impaired migration of mesodermal cells (Furuta et al., 1995). The differences in phenotype between FAK-null and β1de6 homozygotes suggest that the abnormal adhesion-dependent signaling in β1de6/β1de6 MEFs likely does not only depend on the deficit in FAK phosphorylation. Consistently, β1de6 homozygous MEFs showed a defective activation of other important signaling pathways, possibly leading to Rac activation such as those arising from paxillin. The future study of the effects caused by expression of a dominant positive FAK in β1de6 homozygous MEFs will be instrumental to address the specific role of FAK in this system.
Conversely, the rescue of the proliferation defect shown by β1de6 mutant cells by an activated Rac demonstrated a central role of this GTPase in anchorage-dependent signaling. Consistent with this finding, Rac activation has been found to be essential, in endothelial cells, to promote adhesion-dependent Cyclin D1 accumulation and progression through the G1 phase (Mettouchi et al., 2001). The precise mechanism by which Rac controls cell cycle progression is still unclear. Nevertheless, our data indicated that adhesion-induced Rac activation is necessary for the phosphorylation of the Rac downstream effector JNK. The role of JNK in controlling cell proliferation and survival is controversial. However, several experimental evidences suggest its implication in the activation of genes involved in cell cycle progression. For example, fibroblasts lacking the JNK substrate c-Jun show a severe proliferation defect (Johnson et al., 1993). Furthermore, adhesion-dependent JNK activation is necessary for progression through the G1 phase of the cell cycle (Oktay et al., 1999).
It has been previously shown that β1de6 homozygous MEFs normally activate Erk in response to a combined stimulation by adhesion and growth factors (Barberis et al., 2000). However, the results reported here showed that this event is not sufficient to promote cell cycle progression, and that other integrin-dependent signals deriving from Rac are crucially required. The role of adhesion-triggered Erk activation in cell proliferation is indeed controversial: whereas Roovers et al. (1999) propose that artificial activation of Erk in suspended cells can override adhesive requirements for cell cycle progression, Le Gall et al. (1998) show that these conditions are not sufficient to induce Cyclin D1 expression. In addition, recent experiments show that, independently from adhesion, Erk phosphorylation per se is not sufficient for nuclear translocation and activation of the transcription factor Elk (Aplin et al., 2001). Analysis of Erk subcellular localization revealed that, after adhesion to fibronectin, homozygous β1de6 MEFs fail to translocate phosphorylated Erk into the nuclei. Interestingly, this defect could be rescued by the expression of a constitutively activated Rac. Such findings conclusively indicate that Erk nuclear translocation requires adhesion-mediated Rac activation. Further studies will be required to define the precise biochemical mechanism underlying this process.
At present, the possibility that the substitution of the β1 C-terminal tail by 11 amino acids provided a specific ability to positively induce the described signaling defects cannot be formally excluded. However, the chances that the 11 random amino acids result in an intrinsic signaling activity leading to FAK-Rac-JNK/Erk inhibition are objectively poor. In fact, scanning of existing protein domain and human genome databases failed to indicate any significant homology of the β1de6–specific sequence to other naturally occurring proteins. Moreover, the signaling properties of β1de6 are consistent with what described for several different β1 integrin cytoplasmic domain mutants (Sastry and Horwitz, 1993). In particular, the mutant that specifically lacks the alternatively spliced C-terminal tail, like β1de6, does not activate FAK (Retta et al., 1998). Finally, the phenotypic rescue by expression of a constitutively active form of Rac further strengthens the hypothesis that the β1de6 mutation causes a loss of function effect, specifically affecting integrin-mediated signals. In conclusion, the findings presented here provide a genetic link between the β1 integrin cytoplasmic domain and the adhesion-mediated activation of Rac, thus supporting the notion that if integrins fail to trigger signals leading to MAPK activity, cell cycle progression as well as cell survival are impaired in vivo.
To generate the targeting construct aimed at producing a truncated β1 cytoplasmic domain, a 5′ homology arm was obtained by PCR amplification of the intron between murine β1 integrin exon 5 and 6 (1.8 kbp), using two primers hybridizing to the intron downstream exon 5 (5′-GGAATTCCTCGAGCTGAGTCATTGCTGGGGTTC-3′) and to the end of exon 6 (5′-GGAATTCATCAGGTGTCCCACTTGGCATTC-3′), respectively. Stop codons, a PGK polyadenylation sequence, and a neomycin resistance cassette were inserted downstream. The 3′ homology arm consisted in a 4-kbp Hind III-Apa I fragment of the murine β1 gene derived from a 129Sv mouse strain genomic clone. After transfection and selection of G418-resistant R1 ES cells, homologous recombinants were screened by Southern blot, probing XbaI-digested DNA with an Apa I-XbaI genomic fragment external to the construct. A single clone showing the restriction pattern expected by the homologous recombination event was microinjected in C57B6 blastocysts and germ line–transmitting chimeras were used to generate inbred 129Sv and outbred 129Sv/C57B6 mutant strains.
Southern blot analysis of β1de6 heterozygous DNA with a neomycin probe confirmed single copy insertion of the construct but also indicated that the left arm of the targeting construct had integrated downstream the endogenous exon D (Fig. 2 A). This integration event was confirmed by PCR using two oligonucleotides corresponding to exon 6 in forward and reverse direction (P1: 5′-ATTGGGATGATGTCGGGACC-3′; P2: 5′-AGGCGTGGTTGCTGGAATTG-3′) and the Expand Long Template PCR system (Boehringer Mannheim) (Fig. 2 C). The amplified fragment was cloned and sequenced with an ABI-Prism (Perkin Elmer) sequencing machine. The break-point site was identified by comparing this sequence with the sequence of the wild-type intron between exon 5 and 6. These sequences were used for the design of three oligonucleotides (P3: 5′-CTTATGTCCCTATCCTCTGC-3′; P4: 5′-TCTCCCTAGGTGTTTGTACC-3′; P5: 5′-GGCACTTGCTAACAAGACTG-3′) which allowed mouse genotyping by genomic PCR. The P3-P4 and P3-P5 couples amplified the β1 wild-type allele and the β1de6 allele, respectively. RT-PCR experiments (Fig. 2 D) were performed on total RNA of heterozygous ES cells using oligonucleotides located in exon 5 and 6 (5′-AGGACATTGATGACTGCTGG-3′ and P1, respectively).
For the detection of β1A and β1D expression, total RNA was extracted from 10.5-d-old embryos using the RNeasy mini kit (QUIAGEN) and retrotranscribed using random primers (Amersham Pharmacia Biotech). Yolk sacs were used to genotype the embryo. Oligonucleotides were designed to amplify either β1A (P1: 5′-AGGACATTGATGACTGCTGG-3′; P2: 5′-CGGGATCCAAATCCGCCTGAGTAGG-3′), β1D (P1; P3: 5′-AAACTCAGAGACCAGCTTTAC-3′) or actin (5′-TCGACATCAGGAAGGACCTGT-3′; 5′-GAGGTGGAGTGTGTCTAGAAG-3′), in a multiplexed reaction consisting of 10 cycles of touch down protocol (annealing from 65°C to 55°C) followed by 17 cycles (95°C 30 s, 55°C 30 s, and 72°C 30 s) for β1A and 19 cycles for β1D.
To detect wild-type and mutant β1 proteins, embryonic fibroblasts were harvested and washed in Hank's buffer (1.3 mM CaCl2, 0.4 mM MgSO4, 5 mM KCl, 138 mM NaCl, 5.6 mM D-glucose, 25 mM Hepes, pH 7.4). Cells were biotinylated at their surface for 30 min at 4°C, washed three times, lysed in TBS-Triton 0.5%, and centrifuged 10 min. 500 μg of extract were first precipitated with anti-β1A and subsequently with an anti-β1 integrin recognizing the extracellular domain.
Embryos were dissected and fixed in 2% formalin in PBS. Whole-mount specimens were examined and photographed under the stereomicroscope (Wild). For histological analysis, the whole decidua or dissected embryos were fixed in Carnoy's solution for 4–6 h at 4°C. Paraffin sections (5 μm) were analyzed after haematoxylin-eosin staining.
For MEF cultures, 9.5-d-old embryos were dissected from maternal tissue in sterile PBS and individually incubated in trypsin (0.5 ml/embryo). Dissociated tissue was plated in the presence of DME 10% FCS. After 1 wk in culture, cells were expanded to generate a homogeneous cell population. For the subsequent experiments mutant and wild-type cells derived for 2–3 embryos were pooled and passaged maximally five times. Assays were confirmed with at least three different cell populations.
To monitor integrin signaling, cells were deprived of growth factors for 24 h, detached, and washed. The cells were either lysed immediately or plated on fibronectin 15 μg/ml for the indicated times and lysed. Extraction buffer consisted of 25 mM tris-HCl, pH 7.5, 1% Triton X-100, 150 mM NaCl, 1 mM Na3VO4, 10 mM NaF, 1 mM Na4P2O7 and 1 mM EDTA. The Rac pulldown assay was performed as described (Mettouchi et al., 2001).
MEFs were detached with EDTA 3 mM, washed twice, and replated on fibronectin-coated dishes. After a 4-h adhesion, cells were fixed in 2% paraformaldehyde (PFA), permeabilized with TBS 0.3%-Triton, blocked with TBS 5%-BSA, and stained for 1 h with the primary antibody and 45 min with a rodhamine or fluorescein conjugated secondary antibody. For analysis of lamellipodia formation, cells were starved for 24 h, detached and replated as above in the presence of 0.2 FCS and 6.25 μg/ml insulin. After 30 min, cells were fixed in 2% PFA, stained with phalloidin-RITC and bisbenzimide, and photographed. For Erk subcellular localization, MEFs were starved 24 h in the absence or in presence of adenoviral infection (carrying V12 or N17 forms of Rac). Cells were detached with EDTA 3 mM, washed twice and replated on fibronectin coated dishes for 4 h in the presence of growth factors. Cells were fixed with PFA 2%, permeated with TBS 0.3%-Triton, blocked with TBS 5%-BSA, stained ON at 4°C with a polyclonal antibody recognizing Erk (Cat: SC-16; Santa Cruz Biotechnology), washed again in TBS, and stained for 1 h with a rhodamine-conjugated anti–rabbit antibody mixed to a fluorescein-conjugated phalloidin. After TBS washing, cells were incubated 5 min with bisbenzimide (1 μg/ml). Fluorescence was detected using a confocal microscope.
For in vivo BrdU labeling, embryos were dissected from maternal tissues at 8.5 and at 9.5 dpc 1 h after intraperitoneal injection of 10 μg/g body weight of 5-bromo-2′-deoxy-uridine (BrdU; Sigma-Aldrich). For cell proliferation assays, cells were starved 24 h in absence of serum, detached, and replated for 8 h on 15 μg/ml matrix-coated dishes in the presence of 0.2% FCS, 6.25 μg/ml insulin, and 10 μM BrdU. MEFs were fixed with 4% PFA and stained using the BrdU detection kit II (Roche). For FACS analysis of DNA content, cells treated as above were stained with propidium iodide using the Cycle TEST PLUS DNA Reagent Kit (Becton Dickinson). To detect apoptosis, cells or sections of 9.5-dpc embryos were treated in the same way and analyzed by a TUNEL assay using the In Situ Cell Death Detection POD Kit (Roche).
Recombinant adenoviruses expressing GFP or cDNAs for RAC in constitutively active (V12) and dominant negative (N17) forms were constructed. Infection of 293 cells was monitored by GFP expression, and when ~90% of the cells were floating, the pellet was freeze thawed three times to liberate the virus. MEFs were infected by 100 μl of virus stock generating at least the infection of 60% of the cells. E1A expression was monitored to exclude the presence of revertant adenoviruses. GFP-positive infected cells were analyzed for proliferation and survival as above.
Anti P-Tyr PY20, anti-Src, anti-cleaved Caspase-3, anti-Prodomain of Caspase-3, anti-E1A, anti-p27 and anti-Cyclin D1 were purchased from Santa Cruz Biotechnology; anti-paxillin, anti-p85, and anti-RAC were purchased from Transduction Laboratories; and anti-JNK, anti-P-JNK, anti-AKT, anti-P-AKT, anti-p38, and anti-P-p38 were purchased from New England Biolabs.
We wish to thank Sarah Forno and Immacolata Carfora for technical assistance. We are grateful to Bosco Chan (The John P. Roberts Research Institute, London, Canada), Dietmar Vestweber (ZMBE University, Muenster, Germany), and Arnoud Sonnenberg (Netherlands Cancer Institute, Amsterdam, Netherlands) for providing anti-integrin antibodies. We thank Fiorella Balzac, Paola Defilippi and Alexander Pfeifer for helpful discussion.
This work was supported by grants from the National Research Council (Target Project on Biotechnology) and from the Ministry of University and Scientific research to F. Altruda, from AIRC to G. Tarone, and from the Swedish National Science Foundation to R. Fässler.
E. Hirsch and L. Barberis contributed equally to this work.
*Abbreviations used in this paper: dpc, day(s) postcoitum; ES, embryonic stem; FAK, focal adhesion kinase; MEF, murine embryonic fibroblast; PFA, paraformaldehyde; SAPK, stress-activated protein kinase; TUNEL, TdT-mediated dUTP-biotin nick end labelin.