Search tips
Search criteria 


Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1981 May; 146(2): 790–797.
PMCID: PMC217026

Light-induced division and genomic synchrony in phototrophically growing cultures of Rhodopseudomonas sphaeroides.


An experimental procedure for rapidly obtaining cell populations of phototrophically growing Rhodopseudomonas sphaeroides which display division and genomic synchrony has been developed. The basis of the procedure resides with the normal physiological response displayed by cells of R. sphaeroides that have been subjected to an immediate decrease in incident light intensity. After an abrupt high- to low-light transition of an asynchronously dividing cell population, an immediate cessation of increases in culture turbidity, total cell number, and net accumulations of culture deoxyribonucleic acid and phospholipid occurs. Total cell number remains constant for 2.5 h after the transition to low light, after which time, it undergoes a sharp increase. Reinitiation of high-light conditions of growth 1 h subsequent to this increase in total cell number results in a cell population possessing a high degree of division and genomic synchrony. A characterization of this procedure, together with a demonstration of its utility for studies on intracytoplasmic membrane assembly, is presented.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ames GF. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. [PMC free article] [PubMed]
  • BARTLETT GR. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed]
  • BLIGH EG, DYER WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. [PubMed]
  • BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. [PubMed]
  • Cohen LK, Lueking DR, Kaplan S. Intermembrane phospholipid transfer mediated by cell-free extracts of Rhodopseudomonas sphaeroides. J Biol Chem. 1979 Feb 10;254(3):721–728. [PubMed]
  • COHEN-BAZIRE G, SISTROM WR, STANIER RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. [PubMed]
  • Cutler RG, Evans JE. Synchronization of bacteria by a stationary-phase method. J Bacteriol. 1966 Feb;91(2):469–476. [PMC free article] [PubMed]
  • Eccleston ED, Jr, Gray ED. Variations in ppGpp levels in Rhodopseudomonas spheroides during adaptation to decreased light intensity. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1370–1376. [PubMed]
  • Fraley RT, Lueking DR, Kaplan S. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Polypeptide insertion into growing membrane. J Biol Chem. 1978 Jan 25;253(2):458–464. [PubMed]
  • Fraley RT, Lueking DR, Kaplan S. The relationship of intracytoplasmic membrane assembly to the cell division cycle in Rhodopseudomonas sphaeroides. J Biol Chem. 1979 Mar 25;254(6):1980–1986. [PubMed]
  • Goldfine H, Khuller GK, Borie RP, Silverman B, Selick H, 2nd, Johnston NC, Vanderkooi JM, Horwitz AF. Effects of growth temperature and supplementation with exogenous fatty acids on some physical properties of Clostridium butyricum phospholipids. Biochim Biophys Acta. 1977 Sep 28;488(3):341–352. [PubMed]
  • Kosakowski MH, Kaplan S. Topology and growth of the intracytoplasmic membrane system of Rhodopseudomonas spheroides: protein, chlorophyll, and phospholipid insertion into steady-state anaerobic cells. J Bacteriol. 1974 Jun;118(3):1144–1157. [PMC free article] [PubMed]
  • Lessie TG. RNA metabolism of Rhodopseudomonas spheroides during preferential photopigment synthesis. J Gen Microbiol. 1965 Oct;41(1):37–45. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Lueking DR, Fraley RT, Kaplan S. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Fate of "old" and "new" membrane. J Biol Chem. 1978 Jan 25;253(2):451–457. [PubMed]
  • Niederman RA, Mallon DE, Langan JJ. Membranes of Rhodopseudomonas sphaeroides. IV. Assembly of chromatophores in low-aeration cell suspensions. Biochim Biophys Acta. 1976 Aug 13;440(2):429–447. [PubMed]
  • Oelze J, Drews G. Membranes of photosynthetic bacteria. Biochim Biophys Acta. 1972 Apr 18;265(2):209–239. [PubMed]
  • SISTROM WR. The kinetics of the synthesis of photopigments in Rhodopseudomonas spheroides. J Gen Microbiol. 1962 Sep;28:607–616. [PubMed]
  • Takemoto J. Kinetics of photosynthetic membrane protein assembly in Rhodopseudomonas spheroides. Arch Biochem Biophys. 1974 Aug;163(2):515–520. [PubMed]
  • Takemoto J, Huang Kao MY. Effects of incident light levels on photosynthetic membrane polypeptide composition and assembly in Rhodopseudomonas sphaeroides. J Bacteriol. 1977 Feb;129(2):1102–1109. [PMC free article] [PubMed]
  • Wraight CA, Lueking DR, Fraley RT, Kaplan S. Synthesis of photopigments and electron transport components in synchronous phototrophic cultures of Rhodopseudomonas sphaeroides. J Biol Chem. 1978 Jan 25;253(2):465–471. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)