Search tips
Search criteria 


Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1984 April; 158(1): 115–120.
PMCID: PMC215387

Metabolism of 4'-phosphopantetheine in Escherichia coli.


Coenzyme A (CoA) and acyl carrier protein (ACP) contain 4'-phosphopantetheine moieties that are metabolically derived from the vitamin pantothenate. The utilization of metabolites in the biosynthetic pathway during growth was investigated by using an Escherichia coli beta-alanine auxotroph to specifically and uniformly label the pathway intermediates. Pantothenate and 4'-phosphopantetheine were the two intermediates detected in the highest concentration, both intracellularly and extracellularly. The specific cellular content of CoA and ACP was not constant during growth of strain SJ16 (panD) on 4 microM beta-[3-3H]alanine, and alterations in the utilization of 4'-phosphopantetheine and pantothenate correlated with the observed fluctuations of the intracellular pool sizes of CoA and ACP. Double-label experiments indicated that extracellular 4'-phosphopantetheine was derived from the degradation of ACP, and the extent that this intermediate was utilized by 4'-phosphopantetheine adenylyltransferase exerted control over the degradative aspect of the pathway. Control over the biosynthetic aspect of the biochemical pathway was exerted at the level of pantothenate utilization by pantothenate kinase. Reduction in the specific cellular content of CoA and ACP by 4'-phosphopantetheine excretion was irreversible since, in contrast to pantothenate, strain SJ16 was unable to assimilate exogenous 4'-phosphopantetheine into CoA or ACP.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abiko Y, Ashida SI, Shimizu M. Purification and properties of D-pantothenate kinase from rat liver. Biochim Biophys Acta. 1972 May 12;268(2):364–372. [PubMed]
  • Alberts AW, Vagelos PR. Acyl carrier protein. 8. Studies of acyl carrier protein and coenzyme A in Escherichia coli pantothenate or betaalanine auxotrophs. J Biol Chem. 1966 Nov 25;241(22):5201–5204. [PubMed]
  • BROWN GM. The metabolism of pantothenic acid. J Biol Chem. 1959 Feb;234(2):370–378. [PubMed]
  • BROWN GM. Assay and distribution of bound forms of pantothenic acid. J Biol Chem. 1959 Feb;234(2):379–382. [PubMed]
  • Cronan JE., Jr Beta-alanine synthesis in Escherichia coli. J Bacteriol. 1980 Mar;141(3):1291–1297. [PMC free article] [PubMed]
  • Elovson J, Vagelos PR. Acyl carrier protein. X. Acyl carrier protein synthetase. J Biol Chem. 1968 Jul 10;243(13):3603–3611. [PubMed]
  • Forrest WW, Walker DJ. The generation and utilization of energy during growth. Adv Microb Physiol. 1971;5:213–274. [PubMed]
  • Halvorsen O, Skrede S. Regulation of the biosynthesis of CoA at the level of pantothenate kinase. Eur J Biochem. 1982 May;124(1):211–215. [PubMed]
  • HOAGLAND MB, NOVELLI GD. Biosynthesis of coenzyme A from phospho-pantetheine and of pantetheine from pantothenate. J Biol Chem. 1954 Apr;207(2):767–773. [PubMed]
  • Jackowski S, Rock CO. Regulation of coenzyme A biosynthesis. J Bacteriol. 1981 Dec;148(3):926–932. [PMC free article] [PubMed]
  • Jackowski S, Rock CO. Ratio of active to inactive forms of acyl carrier protein in Escherichia coli. J Biol Chem. 1983 Dec 25;258(24):15186–15191. [PubMed]
  • Karasawa T, Yoshida K, Furukawa K, Hosoki K. Feedback inhibition of pantothenate kinase by coenzyme A and possible role of the enzyme for the regulation of cellular coenzyme A level. J Biochem. 1972 Jun;71(6):1065–1067. [PubMed]
  • Kessler SW. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed]
  • MAAS WK. Pantothenate studies. III. Description of the extracted pantothenate-synthesizing enzyme of Escherichia coli. J Biol Chem. 1952 Sep;198(1):23–32. [PubMed]
  • Mäntsälä P. Some characteristics and control of pantothenate transport in Escherichia coli U-5-41. Acta Chem Scand. 1973;27(2):445–452. [PubMed]
  • Powell GL, Elovson J, Vagelos PR. Acyl carrier protein. XII. Synthesis and turnover of the prosthetic group of acyl carrier protein in vivo. J Biol Chem. 1969 Oct 25;244(20):5616–5624. [PubMed]
  • Robishaw JD, Berkich D, Neely JR. Rate-limiting step and control of coenzyme A synthesis in cardiac muscle. J Biol Chem. 1982 Sep 25;257(18):10967–10972. [PubMed]
  • Rock CO. Mixed disulfides of acyl carrier protein and coenzyme A with specific soluble proteins in Escherichia coli. J Bacteriol. 1982 Dec;152(3):1298–1300. [PMC free article] [PubMed]
  • Stokes GB, Stumpf PK. Fat metabolism in higher plants. The nonenzymatic acylation of dithiothreitol by acyl coenzyme A. Arch Biochem Biophys. 1974 Jun;162(2):638–648. [PubMed]
  • Vagelos PR, Larrabes AR. Acyl carrier protein. IX. Acyl carrier protein hydrolase. J Biol Chem. 1967 Apr 25;242(8):1776–1781. [PubMed]
  • Williamson JM, Brown GM. Purification and properties of L-Aspartate-alpha-decarboxylase, an enzyme that catalyzes the formation of beta-alanine in Escherichia coli. J Biol Chem. 1979 Aug 25;254(16):8074–8082. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)