PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. 1986 June; 166(3): 842–848.
PMCID: PMC215203

Role of cell cohesion in Myxococcus xanthus fruiting body formation.

Abstract

Dsp mutants of Myxococcus xanthus have a complex phenotype with abnormal cell cohesion, social motility, and development. All three defects are the result of a single mutation in the dsp locus, a region of DNA about 14 kilobases long. Cohesion appears to play a central role in social motility, since nonsocial mutants exhibit weak agglutination or, in the case of Dsp cells, no agglutination (L. J. Shimkets, J. Bacteriol. 166:837-841, 1986). However, Dsp cells can be agglutinated by cohesive strains of M. xanthus. This provided the opportunity to examine the role of cohesion during development by comparing the developmental phenotype of Dsp cells with that of Dsp cells mixed with cohesive strains. Dsp mutants were unable to complete any of the developmental behaviors: aggregation, fruiting body formation, developmental autolysis, and sporulation. Contact with cohesive strains seemed to restore some developmental characteristics to the Dsp cells. When allowed to develop with wild-type cells, Dsp cells accumulated in fruiting bodies and underwent developmental autolysis, but did not form a significant portion of the spore population. Igl mutants, which may be similar to the previously described frizzy mutants, are cohesive strains that are unable to form fruiting bodies. Mixing Igl cells with Dsp cells under developmental conditions resulted in fruiting body formation, although the Dsp cells were unable to form significant levels of myxospores. In spite of their inability to sporulate under developmental conditions, Dsp mutants did not appear to be defective in the sporulation process. In fact, they formed normal levels of myxospores in response to the chemical inducer glycerol.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Avery L, Kaiser D. In situ transposon replacement and isolation of a spontaneous tandem genetic duplication. Mol Gen Genet. 1983;191(1):99–109. [PubMed]
  • Blackhart BD, Zusman DR. Cloning and complementation analysis of the "Frizzy" genes of Myxococcus xanthus. Mol Gen Genet. 1985;198(2):243–254. [PubMed]
  • Burchard RP, Dworkin M. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J Bacteriol. 1966 Feb;91(2):535–545. [PMC free article] [PubMed]
  • DWORKIN M, GIBSON SM. A SYSTEM FOR STUDYING MICROBIAL MORPHOGENESIS: RAPID FORMATION OF MICROCYSTS IN MYXOCOCCUS XANTHUS. Science. 1964 Oct 9;146(3641):243–244. [PubMed]
  • Geisselsoder J, Campos JM, Zusman DR. Physical characterization of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol. 1978 Feb 25;119(2):179–189. [PubMed]
  • Hagen DC, Bretscher AP, Kaiser D. Synergism between morphogenetic mutants of Myxococcus xanthus. Dev Biol. 1978 Jun;64(2):284–296. [PubMed]
  • Hodgkin J, Kaiser D. Cell-to-cell stimulation of movement in nonmotile mutants of Myxococcus. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2938–2942. [PubMed]
  • Inouye M, Inouye S, Zusman DR. Biosynthesis and self-assembly of protein S, a development-specific protein of Myxococcus xanthus. Proc Natl Acad Sci U S A. 1979 Jan;76(1):209–213. [PubMed]
  • Kaiser D, Dworkin M. Gene transfer to myxobacterium by Escherichia coli phage P1. Science. 1975 Feb 21;187(4177):653–654. [PubMed]
  • Kuner JM, Kaiser D. Introduction of transposon Tn5 into Myxococcus for analysis of developmental and other nonselectable mutants. Proc Natl Acad Sci U S A. 1981 Jan;78(1):425–429. [PubMed]
  • Martin S, Sodergren E, Masuda T, Kaiser D. Systematic isolation of transducing phages for Myxococcus xanthus. Virology. 1978 Jul 1;88(1):44–53. [PubMed]
  • Morrison CE, Zusman DR. Myxococcus xanthus mutants with temperature-sensitive, stage-specific defects: evidence for independent pathways in development. J Bacteriol. 1979 Dec;140(3):1036–1042. [PMC free article] [PubMed]
  • Orndorff PE, Dworkin M. Separation and properties of the cytoplasmic and outer membranes of vegetative cells of Myxococcus xanthus. J Bacteriol. 1980 Feb;141(2):914–927. [PMC free article] [PubMed]
  • Orndorff P, Stellwag E, Starich T, Dworkin M, Zissler J. Genetic and physical characterization of lysogeny by bacteriophage MX8 in Myxococcus xanthus. J Bacteriol. 1983 May;154(2):772–779. [PMC free article] [PubMed]
  • Shimkets LJ. Correlation of energy-dependent cell cohesion with social motility in Myxococcus xanthus. J Bacteriol. 1986 Jun;166(3):837–841. [PMC free article] [PubMed]
  • Shimkets LJ, Kaiser D. Induction of coordinated movement of Myxococcus xanthus cells. J Bacteriol. 1982 Oct;152(1):451–461. [PMC free article] [PubMed]
  • Sodergren E, Cheng Y, Avery L, Kaiser D. Recombination in the Vicinity of Insertions of Transposon Tn 5 in MYXOCOCCUS XANTHUS. Genetics. 1983 Oct;105(2):281–291. [PubMed]
  • White D, Dworkin M, Tipper DJ. Peptidoglycan of Myxococcus xanthus: structure and relation to morphogenesis. J Bacteriol. 1968 Jun;95(6):2186–2197. [PMC free article] [PubMed]
  • Wireman JW, Dworkin M. Morphogenesis and developmental interactions in myxobacteria. Science. 1975 Aug 15;189(4202):516–523. [PubMed]
  • Wu TT. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. [PubMed]
  • Zusman DR. "Frizzy" mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus. J Bacteriol. 1982 Jun;150(3):1430–1437. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)