The Amino-terminal Domain of Desmoplakin Binds to Plakoglobin and Clusters Desmosomal Cadherin–Plakoglobin Complexes

J Cell Biol. 1997 November 3; 139(3): 773–784.

PMCID: PMC2141713

The Amino-terminal Domain of Desmoplakin Binds to Plakoglobin and Clusters Desmosomal Cadherin–Plakoglobin Complexes

Andrew P. Kowalczyk,^‡^§ Elayne A. Bornslaeger,^*^§ Jeffrey E. Borgwardt,^*^§ Helena L. Palka,^*^§ Avninder S. Dhaliwal,^*^§ Connie M. Corcoran,^*^§ Mitchell F. Denning,^*^§ and Kathleen J. Green^*^‡^§

^*Department of Pathology; ^‡Department of Dermatology; and ^§the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, Illinois 60611

Received January 20, 1997; Revised August 8, 1997

This article has been cited by other articles in PMC.

Abstract

The desmosome is a highly organized plasma membrane domain that couples intermediate filaments to the plasma membrane at regions of cell–cell adhesion. Desmosomes contain two classes of cadherins, desmogleins, and desmocollins, that bind to the cytoplasmic protein plakoglobin. Desmoplakin is a desmosomal component that plays a critical role in linking intermediate filament networks to the desmosomal plaque, and the amino-terminal domain of desmoplakin targets desmoplakin to the desmosome. However, the desmosomal protein(s) that bind the amino-terminal domain of desmoplakin have not been identified. To determine if the desmosomal cadherins and plakoglobin interact with the amino-terminal domain of desmoplakin, these proteins were co-expressed in L-cell fibroblasts, cells that do not normally express desmosomal components. When expressed in L-cells, the desmosomal cadherins and plakoglobin exhibited a diffuse distribution. However, in the presence of an amino-terminal desmoplakin polypeptide (DP-NTP), the desmosomal cadherins and plakoglobin were observed in punctate clusters that also contained DP-NTP. In addition, plakoglobin and DP-NTP were recruited to cell–cell interfaces in L-cells co-expressing a chimeric cadherin with the E-cadherin extracellular domain and the desmoglein-1 cytoplasmic domain, and these cells formed structures that were ultrastructurally similar to the outer plaque of the desmosome. In transient expression experiments in COS cells, the recruitment of DP-NTP to cell borders by the chimera required co-expression of plakoglobin. Plakoglobin and DP-NTP co-immunoprecipitated when extracted from L-cells, and yeast two hybrid analysis indicated that DP-NTP binds directly to plakoglobin but not Dsg1. These results identify a role for desmoplakin in organizing the desmosomal cadherin–plakoglobin complex and provide new insights into the hierarchy of protein interactions that occur in the desmosomal plaque.

Desmosomes are highly organized adhesive intercellular junctions that couple intermediate filaments to the cell surface at sites of cell–cell adhesion (Farquhar and Palade, 1963; Staehelin, 1974; Schwarz et al., 1990; Garrod, 1993; Collins and Garrod, 1994; Cowin and Burke, 1996; Kowalczyk and Green, 1996). Desmosomes are prominent in tissues that experience mechanical stress, such as heart and epidermis, and the disruption of desmosomes or the intermediate filament system in these organs has devastating effects on tissue integrity (Steinert and Bale, 1993; Coulombe and Fuchs, 1994; Fuchs, 1994; McLean and Lane, 1995; Stanley, 1995; Bierkamp et al., 1996; Ruiz et al., 1996). Desmosomes are highly insoluble structures that can withstand harsh denaturing conditions (Skerrow and Matoltsy, 1974; Gorbsky and Steinberg, 1981; Jones et al., 1988; Schwarz et al., 1990). This property of desmosomes facilitated early identification of desmosomal components but has impaired subsequent biochemical analysis of the protein complexes that form between desmosomal components. Ultrastructurally, desmosomes contain a core region that includes the plasma membranes of adjacent cells and a cytoplasmic plaque that anchors intermediate filaments to the plasma membrane. The plaque can be further divided into an outer dense plaque subjacent to the plasma membrane and an inner dense plaque through which intermediate filaments appear to loop.

Molecular genetic analysis has revealed that the desmosomal glycoproteins, the desmogleins and desmocollins, are members of the cadherin family of cell–cell adhesion molecules (for review see Buxton et al., 1993, 1994; Cowin and Mechanic, 1994; Kowalczyk et al., 1996). The classical cadherins, such as E-cadherin, mediate calcium-dependent, homophilic cell–cell adhesion (Nagafuchi et al., 1987). The mechanism by which the desmosomal cadherins mediate cell–cell adhesion remains elusive (Amagai et al., 1994; Chidgey et al., 1996; Kowalczyk et al., 1996), although heterophilic interactions have recently been detected between desmogleins and desmocollins (Chitaev and Troyanovsky, 1997). Both classes of the desmosomal cadherins associate with the cytoplasmic plaque protein plakoglobin (Kowalczyk et al., 1994; Mathur et al., 1994; Roh and Stanley, 1995b; Troyanovsky et al., 1994), which is part of a growing family of proteins that share a repeated motif first identified in the Drosophila protein Armadillo (Peifer and Wieschaus, 1990). This multigene family also includes the desmosomal proteins band 6/plakophilin 1, plakophilin 2a and 2b, and p0071, which are now considered to comprise a subclass of the armadillo family of proteins (Hatzfeld et al., 1994; Heid et al., 1994; Schmidt et al., 1994; Hatzfeld and Nachtsheim, 1996; Mertens et al., 1996).

The most abundant desmosomal plaque protein is desmoplakin, which is predicted to be a homodimer containing two globular end domains joined by a central α-helical coiled-coil rod domain (O'Keefe et al., 1989; Green et al., 1990; Virata et al., 1992). Previous studies have demonstrated that the carboxyl-terminal domain of desmoplakin interacts with intermediate filaments (Stappenbeck and Green, 1992; Stappenbeck et al., 1993; Kouklis et al., 1994; Meng et al., 1997), and the amino-terminal domain of desmoplakin is required for desmoplakin localization to the desmosomal plaque (Stappenbeck et al., 1993). Direct evidence supporting a role for desmoplakin in intermediate filament attachment to desmosomes was provided recently when expression of an amino-terminal polypeptide of desmoplakin was found to displace endogenous desmoplakin from cell borders and disrupt intermediate filament attachment to the cell surface in A431 epithelial cell lines (Bornslaeger et al., 1996).

The classical cadherins, such as E-cadherin, bind directly to both β-catenin and plakoglobin (Aberle et al., 1994; Jou et al., 1995; for review see Cowin and Burke, 1996). β-Catenin is also an armadillo family member (McCrea et al., 1991; Peifer et al., 1992), and both plakoglobin and β-catenin bind directly to α-catenin (Aberle et al., 1994, 1996; Jou et al., 1995; Sacco et al., 1995; Obama and Ozawa, 1997). α-Catenin is a vinculin homologue (Nagafuchi et al., 1991) and associates with both α-actinin and actin (Knudson et al., 1995; Rimm et al., 1995; Nieset et al., 1997). Through interactions with β- and α-catenin, E-cadherin is coupled indirectly to the actin cytoskeleton, and this linkage is required for the adhesive activity of E-cadherin (Ozawa et al., 1990; Shimoyama et al., 1992). In addition, E-cadherin association with plakoglobin appears to be required for assembly of desmosomes (Lewis et al., 1997), underscoring the importance of E-cadherin in the overall program of intercellular junction assembly. However, the hierarchy of molecular interactions that couple the desmosomal cadherins to the intermediate filament cytoskeleton is largely unknown, although the desmocollin cytoplasmic domain appears to play an important role in recruiting components of the desmosomal plaque (Troyanovsky et al., 1993, 1994). Since desmosomal cadherins form complexes with plakoglobin and because the amino-terminal domain of desmoplakin is required for desmoplakin localization at desmosomes, we hypothesized that the amino-terminal domain of desmoplakin interacts with the desmosomal cadherin– plakoglobin complex.

In previous studies, we used L-cell fibroblasts to characterize plakoglobin interactions with the cytoplasmic domains of the desmosomal cadherins and found that the desmosomal cadherins regulate plakoglobin metabolic stability (Kowalczyk et al., 1994) but do not mediate homophilic adhesion (Kowalczyk et al., 1996). To test the ability of the desmoplakin amino-terminal domain to interact with the desmosomal cadherin–plakoglobin complex, we established a series of L-cell lines expressing the desmosomal cadherins in the presence or absence of a desmoplakin amino-terminal polypeptide (DP-NTP).¹ The results indicate that one important function of the desmoplakin amino-terminal domain is to cluster desmosomal cadherin–plakoglobin complexes. In addition, DP-NTP and plakoglobin were found to form complexes that could be co-immunoprecipitated from L-cell lysates. Using the yeast two hybrid system, DP-NTP was found to bind directly to plakoglobin but not Dsg1. These data suggest that plakoglobin couples the amino-terminal domain of desmoplakin to the desmosomal cadherins and that desmoplakin plays an important role in organizing the desmosomal cadherin–plakoglobin complex into discrete plasma membrane domains.

Materials and Methods

Cell Culture

L-cells were cultured in Dulbecco's minimal essential medium (DMEM), 10% fetal bovine serum, and penicillin/streptomycin. To establish cell lines, cells were transfected with various cDNA constructs by calcium phosphate precipitation as described previously (Kowalczyk et al., 1994, 1996). The cDNA construct encoding plakoglobin also included a neomycin resistance gene. The antibiotic G418 (GIBCO BRL, Grand Island, NY) was used at 400 μg/ml (active concentration) to select transfected cells. For cells that were not cotransfected with plakoglobin, a plasmid (pSV2_neo) that encoded the neomycin resistance gene, was used as the selection marker. Colonies resistant to G418 were isolated and protein expression was assessed by immunoblot and immunofluorescence. In each case, at least three independently isolated clones expressing the proteins of interest were used for futher characterization. For transient transfections, COS cells were cultured in DMEM, 10% fetal bovine serum, and penicillin/streptomycin and transfected using the calcium phosphate precipitation method as described previously (Stappenbeck and Green, 1992; Kowalczyk et al., 1995). Cells were assayed by immunofluorescence 48 h after transfection. All media and serum were obtained from GIBCO BRL, and tissue culture plasticware was purchased from Becton Dickinson (Lincoln Park, NJ).

cDNA Constructs

Cadherins and Chimeric Cadherins.

cDNA constructs encoding full length human desmoglein-1 (Dsg1) and desmocollin-2a (Dsc2a) were generated as described previously (Kowalczyk et al., 1994, 1996). A chimeric cadherin comprising the extracellular domain of Dsg1 and the intracellular domain of mouse E-cadherin (Dsg1Ecad) was constructed as described previously (Kowalczyk et al., 1994; see Fig. Fig.11 for structure). A second chimera consisting of the extracellular domain of E-cadherin and the cytoplasmic domain of Dsg1 was also generated (EcadDsg1). This chimera comprised the cDNA sequences encoding the extracellular domain of mouse E-cadherin (nucleotides 68–2,190; Nagafuchi et al., 1987) and the cytoplasmic domain of human Dsg1 (nucleotides 1,585–3,072; Nilles et al., 1991). The fusion between E-cadherin and Dsg1 sequences occurs within the transmembrane spanning domain and an 11-amino acid epitope tag from c-myc was added to the carboxyl terminus of the chimera. The E-cadherin portion of the chimera was generated by PCR using the 5′ E-cadherin primer LN37 (5′-TGATTTAAAGTCGACTCCACCATGGGAGCCCGGTGC-3′ to generate a SalI site at the 5′ end of the E-cadherin cDNA and the 3′ E-cadherin primer LN45 (5′-TATAGGTACCGCCAATCCTGCTGCCAC-3′), which generates a 3′ KpnI site within sequences encoding the central region of the membrane spanning domain. The Dsg1 cytoplasmic domain was also generated using PCR. The 5′ Dsg1 primer (LN46: 5′-ATGCGGTACCTCTCATCATGGGATTC-3′) was used to generate a 5′ Kpn I site within the sequences encoding the Dsg1 transmembrane spanning domain in frame with the Kpn I site from the 3′ E-cadherin primer (LN45). A 3′ Dsg1 primer (LN36 GCCAAGCTTCTACAAGTCCTCTTCAGAAATGAGCTTTTGCTCCACCTTGC T A T A T- TGCAC) was used to produce a 3′ HindIII restriction site and the carboxyl-terminal c-myc epitope tag. The PCR products were subcloned into pBluescript (Strategene, La Jolla, CA) and PCR-generated regions were replaced with cloned DNA or sequenced to verify that errors were not introduced into the coding sequence. The KpnI sites were used to ligate the cDNA encoding the extracellular domain of E-cadherin to the cytoplasmic domain of Dsg1. cDNAs encoding Dsg1, Dsc2a, and the Dsg1Ecad chimeras were each subcloned into the RC4B expression vector (Evans and Scarpulla, 1988) for transfection into eukaryotic cells. The EcadDsg1 chimera was subcloned into the mammalian expression vector LK-444, which uses the β-actin promoter as described previously (Kowalczyk et al., 1994).

Figure 1

Schematic representation of cDNA constructs. (A) Full length cDNAs encoding human Dsg1 (Nilles et al., 1991) or Dsc2a (Parker et al., 1991; Kowalczyk et al., 1994) were expressed. Nomenclature for domain structure of the cadherins is adopted from (more ...)

Plakoglobin and Plakoglobin Deletion Mutants.

A full length plakoglobin cDNA was isolated and subcloned into the mammalian expression vector LK-444. A deletion of plakoglobin lacking the amino-terminal domain (PgΔN) was generated by PCR using primers LN123 (5′-ACGCGTCGACCCACGATGCTCAAGTCGGCCATTG-3′) and LN52 (5′-CTGCAGACCACATGCTGGCCGTGGAGCAAAAGCTCATTTCTGAAGAGG- ACTTGTAGAAGCTTGGCC-3′). The resuling PCR product of PgΔN (nucleotides 486–2,354; Franke et al., 1989) contains a SalI restriction site and Kozak consensus sequence at the 5′ end and an 11-amino acid c-myc epitope tag and a HindIII restriction site at the 3′ end. Using the engineered restriction sites, the PgΔN PCR product was inserted into the SalI/ HindIII site in pBluescript and subcloned into the SalI/HindIII sites of the mammalian expression vector LK-444.

A deletion of plakoglobin lacking the carboxyl-terminal domain and a portion of armadillo repeat number 13 (PgΔC) was generated by PCR using primers LN51 (5′-ACGTGTCGACCCACGATGGAGGTGATGAACCTGA-3′) and LN120 (5′-TGTTCCGCATCTCCGAGGTGGAGCAAAAGCTCATTTCTGAAGAGGACTTGTAGAAGCTTGCGCA-3′). The resulting PCR product of PgΔC (nucleotides 120–2,081) contains a SalI restriction site and Kozak consensus sequence at the 5′ end and an 11-amino acid c-myc epitope tag and a HindIII restriction site at the 3′ end. Using the engineered restriction sites, c-myc–tagged PgΔC was subcloned into the SalI/HindIII site in pBluescript and then into the mammalian expression vector LK-444. For each of the plakoglobin deletion constructs, PCR-generated regions were replaced with original cDNA sequences and sequenced to verify that no errors were introduced.

Desmoplakin Amino-terminal Construct.

A cDNA construct encoding a portion of the desmoplakin amino-terminal domain, termed DP-NTP, was constructed and subcloned into a mammalian expression vector using the CMV promoter (Bornslaeger et al., 1996). As previously reported (Bornslaeger et al., 1996), the protein product of the 2.1-kb cDNA is a 70-kD polypeptide, ~24 kD smaller than predicted from the cDNA. DNA sequence analysis indicated the presence of a mutation that arose spontaneously in a precursor plasmid during bacterial replication, which terminates translation at amino acid number 584. Translation read through allows the accumulation of trace amounts of a larger polypeptide (~94 kD), which is present at levels too low to be detected by the NW161 antibody (Bornslaeger et al., 1996).

Immunofluorescence and Electron Microscopy

The distribution of desmosomal proteins in L-cell lines and transiently transfected COS cells was analyzed by immunofluorescence. For most experiments, cells were rinsed in PBS and fixed in −20°C methanol. In some experiments, cells were washed in PBS containing calcium and magnesium (PBS+), incubated in 0.01% saponin for 5 min, rinsed gently in PBS+, and then fixed in −20°C methanol. The following antibodies were used to detect each protein: DP-NTP was detected using polyclonal antibody NW161 directed against an amino-terminal desmoplakin polypeptide (Bornslaeger et al., 1996); Dsg1 was monitored using a human pemphigus foliaceus antibody # 982 (Kowalczyk et al., 1995); Dsc2a was detected using monoclonal antibody 7G6 (Kowalczyk et al., 1994); plakoglobin was monitored using monoclonal antibody 11E4 or a polyclonal antibody (a gift from Dr. J. Papkoff, Megabios Corp. [Burlingame, CA]) directed against a carboxyl-terminal plakoglobin peptide (Hinck et al., 1994; Kowalczyk et al., 1994). The E-cadherin extracellular domain of the EcadDsg1 chimera was detected using rat monoclonal antibodies directed against the E-cadherin extracellular domain (anti-uvomorulin antibody DECMA-1 from Sigma Chemical Co. [St. Louis, MO]) or mAb ECCD-2, a gift from Dr. M. Takeichi, Kyoto University [Kyoto, Japan]). For electron microscopy, cells were grown to confluence on tissue culture grade plasticware or on glass coverslips and processed for conventional electron microscopy as described previously (Green et al., 1991).

Immunoprecipitation and Immunoblot Analysis

Immunoprecipitation was carried out as described (Kowalczyk et al., 1994) with the following changes. L-cells were scraped into Tris-buffered saline containing 0.5% Triton X-100, vortexed, and subjected to centrifugation at 14,000 g. Antibodies directed against Dsg1 (pemphigus serum # 982), the desmoplakin amino terminus (NW161), or preimmune serum from antibody NW161 were incubated with the cell lysate for 2 h at 4°C. Gamma bind plus sepharose beads (Pharmacia Fine Chemicals, Piscataway, NJ) were then added for 2 h at 4°C, immune complexes were captured by centrifugation, and the beads were washed 5 times in Tris-buffered saline containing 0.5% Triton X-100 for 10 min under gentle rotation at 4°C. Immune complexes were released by incubation in reducing SDS-PAGE sample buffer and analyzed by immunoblot using Enhanced Chemiluminescence (Amersham Intl, Arlington Heights, IL). Plakoglobin was detected using mAb 11E4, which is directed against the amino-terminal domain of plakoglobin, or a rabbit polyclonal antibody directed against the carboxyl-terminal domain (Hinck et al., 1994; Kowalczyk et al., 1994).

Yeast Two Hybrid Constructs and Assays

Yeast two hybrid vectors encoding the GAL4 DNA binding (pAS-CYH2; Harper et al., 1993) or transcription activation domain (pACTII; Bai and Elledge, 1995) were generously provided by Dr. Stephen Elledge (Baylor College of Medicine, Houston, TX). The amino-terminal deletion construct of human plakogobin (PgΔN) described above was subcloned from pBluescript into the activation domain construct pACTII. The cDNA in pBluescript was restriction digested with SalI and treated with Klenow fragment of DNA polymerase I to create a blunt 5′ end. The DNA was subsequently digested with EcoRI to remove the PgΔN insert and subcloned into the SmaI and EcoRI sites of pACTII to generate a PgΔN cDNA, which was inserted in frame with upstream sequences encoding the GAL4 transcription activation domain (p515). A construct comprising the cDNA sequences encoding the entire cytoplasmic tail of human Dsg1 in the pAS1-CYH2 DNA binding domain vector was a generous gift from Dr. Michael Klymkowsky (University of Colorado, Boulder, CO). The cDNA sequences encoding Dsg1 were removed from pAS1-CYH2 by digestion with EcoRI and BamHI and subcloned into the same sites of the Path 1 vector (p47), which contains a SalI restriction site 3′ of the BAM HI site. The Dsg1 sequences were then removed with EcoRI and SalI and subcloned into the EcoRI and XhoI sites of pACTII to generate a construct encoding nucleotides 1,633–3,072 of human Dsg1 in frame with upstream sequences encoding the GAL4 activation domain (p504). The cDNA encoding DP-NTP in pBluescript (p552) was restriction digested with BamHI and SalI and subcloned into the BamHI and XhoI sites of pACTII (p597). This construct was subsequently digested with NcoI and SmaI to subclone the DP-NTP sequences into the same restriction sites of the pAS1-CYH2 vector (p601). The first 584 amino acids of human desmoplakin are encoded in both the pACTII and pAS1-CYH2 constructs, similar to the DP-NTP expression vectors described above.

To assay interactions between proteins, yeast (strain HF7c) were transformed with the plasmids of interest and grown on synthetic defined media lacking either tryptophan (SD^−trp) for the pAS1-CYH2 vector or leucine (SD^−leu) for the pACTII vector to select for transformed clones. Transformations and β-galactosidase assays were performed according to methods published in the Matchmaker™ Two Hybrid product protocol (Clontech Laboratories Inc., Palo Alto, CA). Briefly, isolated colonies were transformed with 5 to 10 μg plasmid DNA using a standard lithium acetate/ polyethylene glycol method. Interactions between proteins were tested by streaking transformed colonies onto Whatman filter membranes and assaying β-galactosidase activity using the substrate X-Gal (5-Bromo- 4-chloro-3-indolyl β-d-galactopyranoside; Sigma Chemical Co.). As a second reporter for interactions, colonies were also tested for growth on SD^-leu-trp-his in the presence of 20 mM 3-aminotriazole (Sigma Chemical Co.). Materials for base media and agar were obtained from DIFCO Laboratories (Detroit, MI), and materials for synthetic defined media were purchased from Clontech Laboratories Inc.

Results

The Desmoplakin Amino-terminal Domain Clusters Desmosomal Cadherin–Plakoglobin Complexes

The amino-terminal domain of desmoplakin was previously demonstrated to target desmoplakin to the desmosomal plaque (Stappenbeck et al., 1993; Bornslaeger et al., 1996). We hypothesized that the amino-terminal region of desmoplakin may interact with the desmosomal cadherin– plakoglobin complex, thereby coupling the desmosomal cadherins to the intermediate filament cytoskeleton. To address this question, a series of stable L-cell fibroblast cell lines expressing various desmosomal molecules was established to reconstitute complexes of specific desmosomal components. A schematic diagram depicting the constructs used in this study is shown in Fig. Fig.1.1. L-cell lines expressing Dsg1 and plakoglobin in the presence or absence of DP-NTP were generated, and the expression of each protein was monitored by immunoblot (Fig. (Fig.2).2). An immunoblot for vimentin is also shown to demonstrate that similar levels of protein were loaded in each lane. Parental L-cells (not shown) and neomycin-resistant L-cells do not express detectable levels of desmosomal components (Fig. (Fig.2),2), with the exception of plakoglobin, which is expressed at extremely low levels (Kowalczyk et al., 1994, 1996).

Figure 2

Immunoblot analysis of Dsg1, plakoglobin, and DP-NTP expression in stable L-cell lines. L-cell lines expressing Dsg1 and plakoglobin (L-Dsg1/Pg), Dsg1, plakoglobin and DP-NTP (L-Dsg1/Pg/DP-NTP), or the neomycin resistance marker (L-neo) were analyzed (more ...)

The distribution of exogenously expressed desmosomal proteins in the L-cell lines was monitored by dual label indirect immunofluorescence (Fig. (Fig.3).3). In L-cells expressing Dsg1 and plakoglobin, the proteins colocalized and staining was largely uniform along the plasma membrane (Fig. (Fig.3,3, A and B; see also Kowalczyk et al., 1994). No endogenous desmoplakin staining was detected in these cells (Fig. (Fig.33 D). In contrast, in L-cell lines co-expressing DP-NTP, both Dsg1 (Fig. (Fig.33 E) and plakoglobin (Fig. (Fig.33 F) were present in punctate clusters. In addition, DP-NTP (Fig. (Fig.33 G) colocalized with plakoglobin (Fig. (Fig.33 H), suggesting that all three components were present in a single complex. It is important to note that the clustering of desmosomal cadherins and plakoglobin was observed only in the presence of DP-NTP. Out of a total of 84 L-cell lines expressing Dsg1 and plakoglobin that were generated, clustering was not observed in any of these cell lines (see also Kowalczyk et al., 1996). In contrast, 16 independent clones of L-cell lines expressing the desmoplakin polypeptide were generated, and clustering was observed in all 16 clones. Furthermore, in the representative Dsg1/Pg/DP-NTP cell line shown here, punctate Dsg1 staining was observed in 71 out of 111 cells. This is in comparison to 0 out of 133 cells for the Dsg1/Pg line, indicating that the clustering was due to the co-expression of the desmoplakin polypeptide.

Figure 3

DP-NTP clusters Dsg1 and plakoglobin in L-cell lines. L-cells expressing Dsg1 and plakoglobin (A–D) or Dsg1, plakoglobin, and DP-NTP (E–H) were processed for immunofluorescence. Staining was carried out using antibodies directed against (more ...)

Laser scanning confocal microscopy was used to further characterize the distribution of the punctate clusters in cell lines co-expressing Dsg1, plakoglobin, and DP-NTP. In many instances, punctate clusters of Dsg1 were visualized along the ventral cell surface, as shown in Fig. Fig.44 A, where the focal plane was set at the cell–substrate interface. These clusters colocalized with plakoglobin (not shown), consistent with the results shown in Fig. Fig.3.3. Vertical sections of the cell in Fig. Fig.44 A reveals the presence of clusters on both the ventral and dorsal cell surfaces (Fig. (Fig.44 B). This distribution was observed consistently, as demonstrated by the additional vertical sections shown in Fig. Fig.4,4, C and D. In addition, treatment of cell lines expressing Dsg1, plakoglobin, and DP-NTP with trypsin demonstrated that the Dsg1 was sensitive to trypsin degradation (Fig. (Fig.44 E). The antibody used to detect Dsg1 (Ab NW1) is directed against the Dsg1 cytoplasmic domain (Kowalczyk, A.P., unpublished observations), indicating that the generation of a Dsg1 fragment of ~100 kD in cells treated with trypsin is due to degradation of a portion of the Dsg1 extracellular domain. Together with the observations that the punctate clusters of Dsg1 were observed along the plasma membrane by confocal microscopy, the trypsinization results indicate that the vast majority of Dsg1 was present on the cell surface. This is consistent with a previous report demonstrating that both Dsg1 and Dsc2a are present on the cell surface when expressed in L-cells (Kowalczyk et al., 1995).

Figure 4

Localization of Dsg1 to the cell surface by confocal microscopy and trypsinization. L-cells expressing Dsg1, plakoglobin, and DP-NTP were processed for immunofluorescence microscopy using an antibody (PF sera #982) directed against Dsg1 (A–D (more ...)

DP-NTP Also Clusters Dsc2a–Plakoglobin Complexes

In contrast to adherens junctions, desmosomes contain two distinct subclasses of cadherins, desmogleins and desmocollins. Therefore, the ability of DP-NTP to cause clustering of Dsc2a–plakoglobin complexes was also analyzed (Fig. (Fig.5).5). When expressed alone, Dsc2a (Fig. (Fig.55 A) and plakoglobin (Fig. (Fig.55 B) were distributed in a diffuse, largely uniform staining pattern along the membrane of the L-cells. However, in the presence of DP-NTP, both Dsc2a (Fig. (Fig.55 C) and plakoglobin (Fig. (Fig.55 D) were colocalized in distinct punctate clusters. In addition, DP-NTP (Fig. (Fig.55 E) and plakoglobin (Fig. (Fig.55 F) colocalized in these clusters, indicating that DP-NTP causes clustering of both classes of desmosomal cadherins.

Figure 5

DP-NTP clusters Dsc2a and plakoglobin in L-cell lines. L-cells expressing Dsc2a and plakoglobin (A and B) or Dsc2a, plakoglobin, and DP-NTP (C–F) were processed for immunofluorescence. Staining was carried out using antibodies directed against (more ...)

A Chimeric Cadherin with the E-cadherin Extracellular Domain and the Dsg1 Cytoplasmic Domain (EcadDsg1) Recruits Plakoglobin and DP-NTP to Cell–Cell Borders

A number of previous studies have demonstrated that the desmosomal cadherins do not mediate homophilic adhesion when expressed in L-cells (Amagai et al., 1994; Chidgey et al., 1996; Kowalczyk et al., 1996). A previous study demonstrated that a chimeric cadherin with the E-cadherin extracellular domain and the Dsg3 cytoplasmic domain mediates homophilic adhesion in L-cells (Roh and Stanley, 1995a). Therefore, a chimera containing the E-cadherin extracellular domain and Dsg1 cytoplasmic domain (EcadDsg1) was co-expressed with plakoglobin and DP-NTP to determine if the chimera could recruit plakoglobin and DP-NTP to regions of cell–cell contact. In the absence of DP-NTP, the EcadDsg1 chimera (Fig. (Fig.66 A) and plakoglobin (Fig. (Fig.66 B) were largely diffuse on the plasma membrane, although some areas of cell–cell border staining were observed. However, when this chimera was co- expressed with plakoglobin and DP-NTP, the EcadDsg1 chimera (Fig. (Fig.66 C) and plakoglobin (Fig. (Fig.66 D) were codistributed in a punctate pattern. In addition, the EcadDsg1 chimera and plakoglobin were often localized at regions of cell–cell contact. Furthermore, DP-NTP (Fig. (Fig.66 E) was recruited to cell–cell contacts and colocalized with plakoglobin (Fig. (Fig.66 F). The punctate distribution of DP-NTP at cell–cell interfaces was observed only in L-cells expressing the EcadDsg1 chimera. The observation that the EcadDsg1 chimera, plakoglobin, and DP-NTP were all present at cell– cell borders suggests that all three proteins were present in the same complex and that these complexes were recruited to areas of intercellular contact by the homophilic interactions of the E-cadherin extracellular domain.

Figure 6

A chimeric cadherin with the E-cadherin extracellular domain and the Dsg1 cytoplasmic domain (EcadDsg1) recruits plakoglobin and D-NTP to cell–cell borders. L-cell lines co- expressing the EcadDsg1 chimera and plakoglobin in the absence (A (more ...)

Cell lines expressing the EcadDsg1 chimera and plakoglobin in the presence or absence of DP-NTP were also examined by electron microscopy. In L-cell lines co-expressing DP-NTP, electron-dense regions subjacent to the plasma membrane were observed at regions of cell–cell contact (Fig. (Fig.77 B). Interestingly, these structures are similar to the junctions lacking association with intermediate filaments that were previously reported in A431 cell lines expressing DP-NTP (Fig. (Fig.77 C; Bornslaeger et al., 1996). Although the EcadDsg1 chimera and plakoglobin did colocalize at cell– cell borders in the absence of DP-NTP, the extensive electron-dense plaque-like structures such as those present in cells co-expressing DP-NTP (Fig. (Fig.77 B) were never observed in the absence of DP-NTP (Fig. (Fig.77 A). These data suggest that clustering of the cadherin–plakoglobin complex by DP-NTP leads to the formation of a submembrane plaque that can be identified ultrastructurally and which closely resembles the outer dense plaque of the desmosome.

Figure 7

Electron microscopy of junctional structures assembled by L-cells co-expressing the EcadDsg1 chimera with plakoglobin and DP-NTP. L-cell lines expressing the EcadDsg1 chimera and plakoglobin in the absence (A) or presence (B) of DP-NTP were processed (more ...)

Plakoglobin Bound to the Desmoglein Cytoplasmic Domain Does Not Associate with α-Catenin

In contrast to the desmosomal cadherins, E-cadherin is coupled to the actin cytoskeleton through interactions with α-catenin, which is able to bind directly to both β-catenin and plakoglobin. The ability of plakoglobin to bind to α-catenin and to associate with both desmosomal cadherins and classical cadherins raises the question as to how these two classes of cadherins are coupled to distinct cytoskeletal filament networks. Therefore, we used the EcadDsg1 chimera along with a Dsg1Ecad chimeric cadherin to determine if α-catenin could associate with plakoglobin that is bound to the Dsg1 cytoplasmic domain. The chimeric cadherins were immunoprecipitated from cell lysates using antibodies directed against the extracellular domain of either chimera. Immunoblot analysis of the immunoprecipitated proteins revealed that α-catenin co-immunoprecipitated only with the Dsg1Ecad chimera and did not co-immunoprecipitate with the EcadDsg1 chimera (Fig. (Fig.88 A). However, plakoglobin co-immunoprecipitated with both the Dsg1Ecad and the EcadDsg1 chimeras (Fig. (Fig.88 B). These data indicate that plakoglobin that is associated with the Dsg1 cytoplasmic domain does not bind to α-catenin.

Figure 8

Plakoglobin associates with both the Dsg1Ecad and the EcadDsg1 chimeras, but α-catenin is not associated with the EcadDsg1 chimera. The Dsg1Ecad or the EcadDsg1 chimeras were immunoprecipitated from L-cell lysates under mild detergent conditions (more ...)

Plakoglobin Is Required for DP-NTP to Associate with the Dsg1 Cytoplasmic Domain

In adherens junctions, β-catenin is thought to link E-cadherin to α-catenin by binding directly to both proteins. Due to the fact that plakoglobin and β-catenin are closely related, we reasoned that plakoglobin may couple desmoplakin to the desmosomal cadherin cytoplasmic domains. To test this hypothesis, the distribution of Dsg1 was monitored in L-cell lines expressing DP-NTP in the presence of plakoglobin deletion mutants or in the absence of exogenously expressed plakoglobin (Fig. (Fig.9).9). Similar to control cells expressing full length plakoglobin (see Fig. Fig.3),3), Dsg1 was distributed in punctate clusters in L-cells co-expressing DP-NTP in the presence of plakoglobin deletion mutants lacking either the amino- (Fig. (Fig.99 A) or carboxyl-terminal (Fig. (Fig.99 B) domains (refer to Fig. Fig.11 for deletion map). In the absence of exogenously expressed plakoglobin, Dsg1 (Fig. (Fig.9,9, C and E) was distributed in a diffuse pattern in cells co-expressing Dsg1 and DP-NTP. Very low levels of endogenous plakoglobin are expressed in L-cells (Fig. (Fig.99 D), and only small amounts of endogenous plakoglobin can be detected in L-cells expressing Dsg1 (Kowalczyk et al., 1994). Although DP-NTP was occasionally observed in a punctate staining pattern, DP-NTP was more diffuse (Fig. (Fig.99 F) in the absence of exogenous plakoglobin than in cells co-expressing plakoglobin (see also Figs. Figs.3,3, ,5,5, and and6).6).

Figure 9

Plakoglobin is required for DP-NTP to cluster Dsg1. The distribution of Dsg1 was monitored by immunofluorescence in L-cell lines co-expressing DP-NTP in the presence of plakoglobin deletions lacking the amino (L-Dsg1/PgΔN/DP-NTP; A) or carboxyl (more ...)

To further define the role of plakoglobin in the association of DP-NTP with the desmosomal cadherin cytoplasmic domains, the EcadDsg1 chimera, plakoglobin, and DP-NTP were transiently expressed in COS cells (Fig. (Fig.10).10). When co-expressed in the absence of the chimera, plakoglobin (Fig. (Fig.1010 A) was distributed diffusely in the cytoplasm and DP-NTP (Fig. (Fig.1010 B) was distributed in perinuclear aggregates. COS cells assemble some desmosomes, and low levels of both plakoglobin and desmoplakin staining at cell borders could be detected in transiently transfected cells, presumably reflecting incorporation of the transiently expressed proteins into existing desmosomes (Stappenbeck et al., 1993). However, the vast majority of transiently expressed DP-NTP was not recruited to cell borders by the EcadDsg1 chimera in the absence of plakoglobin (Fig. (Fig.10,10, A and B). In COS cells cotransfected with the EcadDsg1 chimera and DP-NTP, the EcadDsg1 chimera accumulated at cell–cell borders (Fig. (Fig.1010 C), whereas DP-NTP remained in perinuclear aggregates (Fig. (Fig.1010 D). In contrast, in cells transiently expressing plakoglobin, DP-NTP, and the EcadDsg1 chimera, the EcadDsg1 chimera (Fig. (Fig.1010 E) colocalized at borders with both plakoglobin (not shown) and DP-NTP (Fig. (Fig.1010 F). These findings are consistent with the results observed in L-cell lines and demonstrate that plakoglobin is required for the EcadDsg1 chimera to recruit DP-NTP to areas of cell–cell contact.

Figure 10

Plakoglobin is required for the EcadDsg1 chimera to recruit DP-NTP to cell–cell borders in transiently transfected COS cells. When co-expressed transiently in COS cells, plakoglobin (A) was largely diffuse in the cytoplasm and DP-NTP (B) was (more ...)

Plakoglobin Forms Complexes with DP-NTP in L-cells and Binds Directly to DP-NTP

Co-immunoprecipitation experiments were conducted to determine if protein complexes could be identified in L-cells that exhibited clustered cadherin–plakoglobin complexes. L-cells co-expressing Dsg1, DP-NTP, and plakoglobin or deletion mutants of plakoglobin were solubilized in 0.5% Triton X-100, and immunoprecipitations were carried out using an antibody directed against either Dsg1 or DP-NTP (Fig. (Fig.11).11). Plakoglobin or plakoglobin deletion mutants were then detected using antibodies directed against the amino- (PgNT) or carboxyl-terminal domain (PgCT) of plakoglobin. The results indicate that plakoglobin and the plakoglobin deletion mutants co-immunoprecipitate with both Dsg1 and DP-NTP. Preimmune sera from antibody NW161 was used as a control to verify that plakoglobin was co- immunoprecipitated specifically with DP-NTP. These data demonstrate that plakoglobin is in a complex with DP-NTP in the L-cell lines. In addition, the fact that plakoglobin deletion mutants lacking either the amino- or carboxyl-terminal end domains of plakoglobin also co-immunoprecipitated with DP-NTP suggests that the central armadillo repeats of plakoglobin are important for association with both Dsg1 and DP-NTP.

Figure 11

Plakoglobin co-immunoprecipitates with DP-NTP. Dsg1 or DP-NTP were immunoprecipitated from L-cell lines co-expressing Dsg1, DP-NTP, and full length plakoglobin or plakoglobin deletion mutants. The immunoprecipitates were then analyzed by immunoblot (more ...)

To determine if plakoglobin and DP-NTP interact directly, these proteins were expressed in the yeast two hybrid system and interactions were analyzed by monitoring β-galactosidase activity (Fig. (Fig.12,12, A–D). Cotransformation of plasmids containing the interacting domains from p53 and large T antigen (p53_DB and LT_TA) were used as positive controls for transcription activation (Fig. (Fig.1212 A). Control experiments were also conducted to verify that the DP-NTP (DP-NTP_DB) and plakoglobin constructs (PGΔN_TA) did not spontaneously activate transcription. No β-galactosidase activity was observed when PGΔN_TAwas tested for interaction with p53_DB (Fig. (Fig.1212 B). However, β-galactosidase activity was consistently observed when yeast were cotransformed with PGΔN_TA and DP-NTP_DB (Fig. (Fig.1212 C), indicating that these proteins interact directly. Although DP-NTP_DB was found to interact with PGΔN_TA (Fig. (Fig.1212 C), an interaction between DP-NTP_DB and the Dsg1 cytoplasmic tail (Dsg1_TA) was not detected (Fig. (Fig.1212 D). Similar to previous studies (Witcher et al., 1996), the Dsg1 cytoplasmic tail exhibited strong interactions with a PGΔN-DNA– binding domain fusion, indicating that the Dsg1 construct was functional (not shown). Although DP-NTP did not activate transcription in the DNA-binding domain vector, PGΔN in the DNA-binding domain construct did exhibit low levels of transcription activation, precluding our ability to test PGΔN interaction with DP-NTP in the reverse orientation. However, these results were confirmed using growth in the absence of histidine as a second, independent reporter for the activation of transcription. Yeast cotransformed with DP-NTP_DB and PGΔN_TA exhibited growth on agar plates lacking histidine in the presence of 20 mM 3-aminotriazole (Fig. (Fig.1212 G), whereas no growth was detected in parallel experiments in which the Dsg1 tail was tested for interactions with DP-NTP (Fig. (Fig.1212 H). These data indicate that DP-NTP binds directly to plakoglobin but not Dsg1.

Figure 12

Plakoglobin interacts directly with DP-NTP in the yeast two hybrid system. Colonies of yeast strain HF7c were transformed with the indicated constructs, and colonies were assayed for β-galactosidase activity (A–D) or the ability to grow (more ...)

Discussion

The molecular interactions that couple the desmosomal cadherins to the intermediate filament cytoskeleton have not been well characterized. Previous studies had shown that the amino-terminal domain of desmoplakin is important for localizing desmoplakin to the cytoplasmic plaque of the desmosome (Stappenbeck et al., 1993; Bornslaeger et al., 1996). In the current study, we sought to determine if desmosomal cadherin–plakoglobin complexes interact with the amino-terminal domain of desmoplakin. The results indicate that the amino-terminal domain of desmoplakin clusters desmosomal cadherin–plakoglobin complexes and binds directly to plakoglobin, but not desmoglein. These results provide new insights into the hierarchy of molecular interactions that occur in the cytoplasmic plaque of the desmosome.

In L-cell lines co-expressing DP-NTP, both Dsg1- (Fig. (Fig.3)3) and Dsc2a–plakoglobin (Fig. (Fig.5)5) complexes are clustered into punctate regions of staining, which appear to be at the plasma membrane. It is interesting to note that the desmosomal cadherins are clustered in L-cells co-expressing DP-NTP, even though these cells do not aggregate in suspension (not shown). The fact that the desmosomal cadherins are clustered but are not engaged in adhesion in L-cells indicates that clustering can occur independent of adhesion. These observations are consistent with the finding that desmosomal complexes resembling half desmosomes are assembled in HaCaT keratinocytes grown in low calcium medium (Demlehner et al., 1995). Clustering of desmosomal cadherins and plakoglobin represents only one aspect of desmosome assembly, and it will be interesting to determine if other desmosomal components such as pinin (Ouyang and Sugrue, 1992, 1996), envoplakin (Ruhrberg et al., 1996), and the plakophilins require desmosomal cadherin-mediated adhesion for assembly into the plaque.

Co-immunoprecipitation experiments demonstrated that plakoglobin and DP-NTP are present in complexes that can be extracted from L-cell lysates (Fig. (Fig.11).11). Although we could not detect the desmosomal cadherins in these complexes, it is likely that the complexes that form between DP-NTP and plakoglobin do contain the cadherins. This is supported by the fact that the desmosomal cadherins are clustered by DP-NTP along with plakoglobin. Furthermore, in cell lines expressing the EcadDsg1 chimera, plakoglobin and DP-NTP are both redistributed to cell–cell interfaces and colocalize with the chimera. These observations strongly suggest that the desmosomal cadherins, plakoglobin, and DP-NTP exist in the same complex.

In addition to the observation that plakoglobin and DP-NTP co-immunoprecipitate, yeast two hybrid analysis demonstrated that DP-NTP binds directly to plakoglobin, but not Dsg1 (Fig. (Fig.12).12). The lack of DP-NTP binding to Dsg1 in the two hybrid system is consistent with the observation that Dsg1 clustering did not occur in L-cell lines in the absence of plakoglobin and with the observation that DP-NTP was not recruited to COS cell–cell borders with the EcadDsg1 chimera in the absence of plakoglobin (Fig. (Fig.10).10). These results lead us to propose that plakoglobin couples desmoplakin to the desmosomal cadherins, thereby linking these cadherins to the intermediate filament cytoskeleton. Previous studies by Troyanovsky et al. (1993, 1994) demonstrated that a connexin-Dsc2a chimera recruited endogenous desmoplakin to the plasma membrane, even when the plakoglobin-binding region of the Dsc2a tail was deleted. The data presented here do not rule out the possibility that desmoplakin binds to desmocollin directly, or that other proteins in addition to plakoglobin can facilitate desmoplakin–desmosomal cadherin interactions. However, our data indicate that plakoglobin plays an important role in linking desmoplakin to the desmoglein cytoplasmic domain. In the desmosome, therefore, plakoglobin may play a role analogous to the adherens junction protein β-catenin, which couples the classical cadherins to α-catenin (Aberle et al., 1994; Jou et al., 1995; Cowin and Burke, 1996).

Recently, transgenic mice with a null mutation in the plakoglobin gene were reported to have defects in desmosome assembly in the intercalated discs of the heart (Bierkamp et al., 1996; Ruiz et al., 1996). In these mice, Dsg2 no longer clustered into distinct junctional structures but exhibited a diffuse distribution on the cell surface. In addition, normal desmosomes were no longer detected in the intercalated discs of the plakoglobin null mice, and adherens junctions and desmosomal components appeared to be mixed into the same junctional structures. Interestingly, desmoplakin localized in these structures, suggesting that this molecule can associate with other junctional proteins in addition to plakoglobin. A family of proteins related to plakoglobin, termed plakophilins, has been identified, and the desmosomal component originally termed band 6 is now known to be a plakophilin family member (Hatzfeld et al., 1994; Heid et al., 1994; Hatzfeld and Nachtsheim, 1996; Mertens et al., 1996). It is possible that plakophilin family members or other desmosomal components also play a role in linking the desmosomal cadherins to desmoplakin.

An interesting property of plakoglobin is that it binds to both classical and desmosomal cadherins. The observation that DP-NTP binds to plakoglobin and clusters desmosomal cadherin–plakoglobin complexes implies that desmoplakin binds to plakoglobin that is associated with the desmosomal cadherin cytoplasmic domain. In contrast, plakoglobin that is associated with the desmosomal cadherin cytoplasmic domain is unable to bind to α-catenin (Fig. (Fig.8;8; Plott et al., 1994; Roh and Stanley, 1995a). Recent studies have demonstrated that the α-catenin and Dsg1 binding sites on plakoglobin overlap in the amino-terminal armadillo repeats of plakoglobin, suggesting that Dsg1 and α-catenin cannot bind to the same plakoglobin molecule simultaneously (Sacco et al., 1995; Aberle et al., 1996; Chitaev et al., 1996; Troyanovsky et al., 1996; Witcher et al., 1996). However, the classical cadherins bind to the central armadillo repeats of plakoglobin, leaving the amino-terminal armadillo repeats of plakoglobin available to bind α-catenin. In the present study, deletion mutants of plakoglobin lacking either the amino- or carboxyl-terminal domain co-immunoprecipitated with DP-NTP and were clustered in L-cells co-expressing DP-NTP. These results, along with the ability of the amino-terminal plakoglobin deletion to interact with DP-NTP in the two hybrid system, suggest that desmoplakin may bind to the central armadillo repeats of plakoglobin. In A431 cells, the expression of plakoglobin deletion mutants lacking the amino- and carboxyl-terminal end domains promoted the assembly of elongated and fused desmosomes, suggesting that the central armadillo repeats of plakoglobin can promote interactions between desmosomal proteins, which may include desmoplakin (Palka and Green, 1997). Future studies will be directed at mapping precisely where desmoplakin binds to plakoglobin in relation to the sites on plakoglobin that bind to the desmosomal cadherins. Such studies should provide insight into the mechanisms by which the cadherin cytoplasmic tails govern the domains on plakoglobin that are available for interactions with either α-catenin or desmoplakin.

Acknowledgments

The authors would like to thank Drs. M. Wheelock, K. Johnson, J. Papkoff, M. Udey, M. Takeichi, K. Trevor, and J. Stanley for sharing antibody –and cDNA reagents that made this work possible. Thanks also to Drs. S. Elledge and M. Klymkowsky for generously providing yeast two hybrid reagents and expertise. We also would like to thank S. Norvell for assistance with the confocal microscopy, D. Kopp for assistance with the COS transfections, S. Guy for cDNA sequence analysis, and K. Myung and M. Moody for assistance preparing samples for electron microscopy.

This work was supported by the National Institutes of Health (RO1AR43380 and RO1AR41836) and a March of Dimes Birth Defects Foundation grant (1-FY96-0146) to K.J. Green. A.P. Kowalczyk was supported by a postdoctoral fellowship from the Dermatology Foundation, and K.J. Green is a Faculty Research Award recipient of the American Cancer Society.

Footnotes

Address

all correspondence to Kathleen Green, Department of Pathology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611. Tel.: (312) 503-5300. Fax: (312) 503-8240.

Abbreviation used in this paper: DP-NTP, desmoplakin amino-terminal polypeptide.

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