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J Exp Med. 1974 March 1; 139(3): 600–616.
PMCID: PMC2139548




A rat basophilic leukemia cell line originally described by Eccleston et al. (3) has been successfully transplanted into eight Wistar strains and adapted to suspension cell culture without noticeable morphological changes. The cells deplete the reaginic activity of mouse and rat immune sera to an extent roughly equivalent to that reported for normal rat mast cells. Studies utilizing radioiodinated antilight-chain antibodies and radioiodinated partially purified rat IgE indicate that the cells bind IgE to their surface membrane with high specificity. Heating or mildly reducing the rat IgE destroyed its binding activity. The binding is unaffected by the presence or absence of Ca++ and Mg++, and is markedly inhibited by reaginic but not by normal rat or mouse serums. The affinity of these cells for human IgE, if present at all, is substantially lower than for rat IgE.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Stanworth DR, Humphrey JH, Bennich H, Johansson SG. Inhibition of Prausnitz-Küstner reaction by proteolytic-cleavage fragments of a human myeloma protein of immunoglobulin class E. Lancet. 1968 Jul 6;2(7558):17–18. [PubMed]
  • Ishizaka K, Ishizaka T, Lee EH. Biologic function of the Fc fragments of E myeloma protein. Immunochemistry. 1970 Aug;7(8):687–702. [PubMed]
  • Eccleston E, Leonard BJ, Lowe JS, Welford HJ. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat New Biol. 1973 Jul 18;244(133):73–76. [PubMed]
  • FURTH J, HAGEN P, HIRSCH EI. Transplantable mastocytoma in the mouse containing histamine, heparin, 5-hydroxytryptamine. Proc Soc Exp Biol Med. 1957 Aug-Sep;95(4):824–828. [PubMed]
  • DUNN TB, POTTER M. A transplantable mast-cell neoplasm in the mouse. J Natl Cancer Inst. 1957 Apr;18(4):587–601. [PubMed]
  • Levine BB, Vaz NM. Effect of combinations of inbred strain, antigen, and antigen dose on immune responsiveness and reagin production in the mouse. A potential mouse model for immune aspects of human atopic allergy. Int Arch Allergy Appl Immunol. 1970;39(2-3):156–171. [PubMed]
  • NISONOFF A, MARKUS G, WISSLER FC. Separation of univalent fragments of rabbit antibody by reduction of a single, labile disulphide bond. Nature. 1961 Jan 28;189:293–295. [PubMed]
  • McConahey PJ, Dixon FJ. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. [PubMed]
  • Minard P, Levy DA. Reaginic antibody receptors on murine mastocytoma cells. J Immunol. 1972 Oct;109(4):887–890. [PubMed]
  • Ishizaka T, Ishizaka K, Tomioka H. Release of histamine and slow reacting substance of anaphylaxis (SRS-A) by IgE-anti-IgE reactions on monkey mast cells. J Immunol. 1972 Feb;108(2):513–520. [PubMed]
  • PATON WDM. Compound 48/80: a potent histamine liberator. Br J Pharmacol Chemother. 1951 Sep;6(3):499–508. [PubMed]
  • Bach MK, Brashler JR. On the nature of the presumed receptor for IgE on mast cells. II. Demonstration of the specific binding of IgE to cell-free particulate preparations from rat peritoneal mast cells. J Immunol. 1973 Aug;111(2):324–330. [PubMed]
  • Bach MK, Block KJ, Austen KF. IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. II. Interaction of IgGa and IgE at the targe cell. J Exp Med. 1971 Apr 1;133(4):772–784. [PMC free article] [PubMed]
  • Becker KE, Ishizaka T, Metzger H, Ishizaka K, Grimley PM. Surface IgE on human basophils during histamine release. J Exp Med. 1973 Aug 1;138(2):394–409. [PMC free article] [PubMed]
  • Perelmutter L, Liakopoulou A. Detection of IgE mediated immediate hypersensitivity reactions in the sera of ragweed sensitive individuals using rat mast cells. Int Arch Allergy Appl Immunol. 1971;40(4-5):481–494. [PubMed]
  • Gillman SA, Haddad ZH. Histamine release from rat peritoneal mast cells after passive sensitization with human reaginic serum or E myeloma protein. J Allergy Clin Immunol. 1972 Sep;50(3):131–137. [PubMed]
  • Plaut M, Lichtenstein LM, Bloch KJ. Failure to obtain histamine release from rat mast cells exposed to human allergic serum and specific antigen or IgE myeloma protein and anti-IgE. J Immunol. 1973 Oct;111(4):1022–1030. [PubMed]
  • Bach MK, Brashler JR, Stechschulte DJ. Isolation of hapten-specific antibodies from reagin-rich rat sera and their use for the preparation of rat peritoneal cells for antigen-mediated histamine release. Immunochemistry. 1973 May;10(5):305–311. [PubMed]
  • Vaz NM, Ovary Z. Passive anaphylaxis in mice with gamma-G antibodies. 3. Release of histamine from mast cells by homologous antibodies. J Immunol. 1968 May;100(5):1014–1019. [PubMed]
  • Bach MK, Bloch KJ, Austen KF. IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. I. Optimum conditions for in vitro preparation of target cells with antibody and and challenge with antigen. J Exp Med. 1971 Apr 1;133(4):752–771. [PMC free article] [PubMed]
  • Osler AG, Lichtenstein LM, Levy DA. In vitro studies of human reaginic allergy. Adv Immunol. 1968;8:183–231. [PubMed]
  • Ishizaka K, Ishizaka T, Menzel AE. Physicochemical properties of reaginic antibody. VI. Effect of heat on gamma-E-, gamma-G- and gamma-A-antibodies in the sera of ragweed sensitive patients. J Immunol. 1967 Sep;99(3):610–618. [PubMed]

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