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Figure 8

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Calcium-induced release of Ca2+ and its mitochondrial sequestration. Cells were coloaded with rhod-2 and Calcium Green and examined in perforated patch as in Fig. Fig.4.4. (A) Calibrated (left) and aligned (right) Cam and Cac records are from a single cell subjected to sequential depolarizations of 50 (diamond), 100 (box), 250 (circle), and 500 (triangle) ms depolarizations, applied at the arrows. Results are representative of four similar experiments. (B) Averaged responses from four cells treated exactly as above. The enlarged, closed symbols mark the observed or interpolated Cam and Cac values at the termination of stimulus.

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