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Loss of the tumor suppressor gene von Hippel–Lindau (VHL) plays a key role in the oncogenesis of clear cell renal cell carcinoma (CCRCC). The loss leads to stabilization of the HIF transcription complex, which induces angiogenic and mitogenic pathways essential for tumor formation. Nonetheless, additional oncogenic events have been postulated to be required for the formation of CCRCC tumors. Here, we show that the Notch signaling cascade is constitutively active in human CCRCC cell lines independently of the VHL/HIF pathway. Blocking Notch signaling resulted in attenuation of proliferation and restrained anchorage-independent growth of CCRCC cell lines. Using siRNA targeting the different Notch receptors established that the growth-promoting effects of the Notch signaling pathway were attributable to Notch-1 and that Notch-1 knockdown was accompanied by elevated levels of the negative cell-cycle regulators p21Cip1 and/or p27Kip1. Treatment of nude mice with an inhibitor of Notch signaling potently inhibited growth of xenotransplanted CCRCC cells. Moreover, Notch-1 and the Notch ligand Jagged-1 were expressed at significantly higher levels in CCRCC tumors than in normal human renal tissue, and the growth of primary CCRCC cells was attenuated upon inhibition of Notch signaling. These findings indicate that the Notch cascade may represent a novel and therapeutically accessible pathway in CCRCC.
Each year, renal cell carcinoma (RCC) afflicts about 150,000 people globally and accounts for nearly 78,000 deaths (1). Although recent years have yielded important information about the underlying molecular mechanisms of renal oncogenesis, nephrectomy still remains the basis of treatment for RCCs. Accordingly, effective therapy for patients with metastatic advanced-stage RCC is limited, though recent data show that treatment with multikinase inhibitors significantly prolongs progression-free survival (2, 3).
Clear cell RCC (CCRCC), which accounts for approximately 75% of all RCCs, is characterized by inactivation of the von Hippel–Lindau (VHL) tumor suppressor gene (1). Reintroduction of functional VHL protein (pVHL) into VHL-negative CCRCC cells totally suppressed their capacity to form tumors in nude mice (4). The tumor suppressive function of pVHL has been attributed to its role in a multiprotein complex that targets the α-subunits of the HIFs (HIF-1α and HIF-2α) for degradation in an oxygen-dependent manner (5–7). HIF-1α and HIF-2α are transcription factors that are instrumental in cellular responses to hypoxia. Loss of pVHL leads to oxygen-independent stabilization of HIF-α subunits and upregulation of a diverse range of HIF targets that regulate metabolism, glucose transport, proliferation, and angiogenesis (8). Of the 2 HIF-α isoforms, HIF-2α is the key mediator in the tumorigenic process of CCRCCs (5), most likely due to different functional characteristics of the HIF-α proteins (9–11). Clearly, HIF-α stabilization is instrumental in tumor formation, but based on genetic and molecular studies, it has been postulated that unknown additional tumorigenic events are required for the genesis of CCRCCs (12, 13).
Notch signaling is critical for determination of cell fates within a wide variety of tissues by regulation of growth, differentiation, and apoptosis. The 4 mammalian Notch receptors (Notch-1 to Notch-4) are single-pass, heterodimeric transmembrane proteins that serve as receptors for the Delta-like (Dll-1, Dll-3, and Dll-4) and Jagged (Jagged-1 and Jagged-2) ligands expressed on neighboring cells (14). Ligand binding leads to 2 subsequent proteolytic cleavages that release the intracellular domain of Notch (icNotch), which then transits to the nucleus, where it converts the transcriptional repressor CBF1/suppressor of hairless/Lag-1 to an activator (15). The principal transcriptional outputs of the Notch cascade are a set of basic helix-loop-helix factors of the hairy and enhancer of split (Hes) and Hes-related repressor protein (Hey) families (16). Importantly, the second cleavage is mediated by the γ-secretase complex, and effective inhibition of Notch activation can be achieved using pharmacological inhibitors of this proteolytic activity. The association between dysregulation of the Notch pathway and human cancer is firmly established in T cell acute lymphoblastic leukemias, in which point mutations or chromosomal translocations of the Notch-1 receptor lead to constitutive signaling (17, 18). Accumulating data indicate that dysregulated Notch activity is also involved in the genesis of other human cancers, such as melanoma, glioma, breast cancer, pancreatic cancer, medulloblastoma, and colorectal, cervical, and mucoepidermoid carcinomas (19, 20). In these tumors, the oncogenic effect of Notch signaling reflects an aberrant recapitulation of the highly tissue-specific function of the cascade during normal development and in tissue homeostasis, where, in most cases, active Notch signaling maintains the cells in an immature, proliferating state.
There are several recent studies showing that the Notch signaling pathway has an important role during development of the mammalian kidney. Several key members of the Notch cascade are expressed during nephrogenesis (21). Inhibition of Notch signaling results in a decrease of the epithelial compartment within the developing mouse kidney, with a particular reduction of the proximal tubules (22), the tissue from which CCRCC is thought to arise (23). Furthermore, targeted mutation of Notch-2 in mice leads to severe defects in kidney development (24, 25).
Hypoxia attenuates differentiation of muscle and neuronal progenitor cells in a Notch-dependent manner. These observations were explained by the finding that HIF-1α interacts with icNotch-1 and enhances the expression of Notch target genes (26). Furthermore, we have shown that Notch signaling is elevated in hypoxic neuroblastoma cells, which might contribute to the immature phenotype of this tumor (27, 28).
In light of the important function of Notch signaling in renal development, in combination with studies showing a crosstalk between the Notch and VHL/HIF pathways, we investigated the role of Notch signaling in CCRCC. Our results show that the components of the Notch pathway are expressed and active in CCRCC independent of the function of VHL and that Notch inhibition perturbs growth of CCRCC cells in vitro. Importantly, we could show that intermittent treatment with a γ-secretase inhibitor effectively inhibited CCRCC tumor growth in vivo and that this treatment regime minimized the well-known adverse effect on goblet cell differentiation associated with systemic Notch inhibition. Together, these results show that the Notch pathway might represent a previously unappreciated therapeutic opportunity for treatment of CCRCCs.
To address whether CCRCC cells express Notch signaling components, we performed Western blot experiments using extracts from a panel of CCRCC cell lines. The cell lines investigated expressed either HIF-2α only (SKRC-21, SKRC-17) or both HIF-1α and HIF-2α (SKRC-7, SKRC-10, SKRC-52), as shown in Figure Figure1A1A and as previously reported (29). The CCRCC cell line Caki-2, which expresses wild-type pVHL (30), did not express HIF-2α. Low expression of HIF-1α was, for unknown reasons, however, detected in this cell line, as reported elsewhere (30). Jagged-1 and Notch-1 expression was detected in all cell lines investigated. Furthermore, expression of the primary Notch downstream target Hes-1 was detected at varying levels in all cell lines examined (Figure (Figure1A).1A). It should be noted that 2 of the cell lines (SKRC-17 and SKRC-52) are derived from metastatic lesions (31). Using quantitative real-time PCR (Q-PCR), we detected expression of Jagged-1, Jagged-2, Notch-1, Notch-2, Hes-1, and Hey-1 mRNAs in all cell lines investigated (Figure (Figure1B),1B), while the expression of Dll-1, Dll-3, Notch-3, and Hey-2 was below detection. Taken together, these results show that the expression of Notch ligands, receptors, and downstream targets is a general characteristic of CCRCC cells, seemingly independent of both VHL status and expression of either of the 2 HIF-α isoforms.
To further clarify whether the Notch cascade is expressed independently of the VHL/HIF axis, we employed the VHL-negative CCRCC cell line 786-O and subclones transfected with empty (PRC3) or VHL-expressing vector (WT7), which have been extensively studied with regard to the tumor suppressor function of pVHL, both in vivo and in vitro (4, 5). As previously reported (7), the 786-O and PRC3 cells expressed high levels of HIF-2α due to the absence of pVHL, while no expression could be detected in the WT7 cells (Figure (Figure1C).1C). The Notch-1 receptor was expressed at equal levels in 786-O, PRC3, and WT7 cells (Figure (Figure1C).1C). Expression of Jagged-1 and Hes-1 was readily detected in the 786-O and PRC3 cells, and a modest elevation of the expression was detected in the pVHL-reconstituted WT7 cells (Figure (Figure1C).1C). To exclude clonal variations of the PRC3 and WT7 cells, a series of independent pVHL-reconstituted clones were analyzed by immunoblotting, verifying that Hes-1 expression was not substantially affected by presence or absence of pVHL (data not shown). Q-PCR analyses confirmed that the established HIF target gene VEGF was expressed at substantially lower levels in the WT7 clone expressing pVHL compared with the control clone and 786-O (Figure (Figure1D).1D). Furthermore, the expression of the Notch target genes Hes-1 and Hey-1 was elevated in pVHL-reconstituted cells. Thus, reexpression of pVHL does not correlate with a marked decrease of Notch signaling, which would have been expected if a HIF-mediated potentiation of Notch signaling, as reported in other cell systems (26), were at hand in CCRCC cells.
It is known that HIF-α transcriptional activity is regulated by oxygen-dependent hydroxylation of the transactivating domain by FIH-1 (12, 32). We therefore also compared the expression of the Notch signaling components in PRC3 and WT7 cells at normoxia and hypoxia in order to elucidate whether an effect on this pathway could be detected in a hypoxic context. In addition, we also ablated HIF-2α expression using siRNA in this experimental setup. We could confirm the efficacy of the siRNA in both clones and the restoration of the hypoxic response in WT7 cells (Figure (Figure1E).1E). No substantial differences in expression of Jagged-1, Notch-1, and Hes-1 could be detected, irrespective of the oxygenation status of the cells or the absence or presence of HIF-2α (Figure (Figure1E).1E). Since 786-O cells and the derivative clones only express HIF-2α (7), we also assessed the effect of HIF-1α knockdown at normoxia and hypoxia in the SKRC-10 cell line, which expresses both HIF-1α and HIF-2α (Figure (Figure1A).1A). While the HIF-1α target carbonic anhydrase IX (CAIX) (29) clearly was downregulated in cells transfected with siRNA directed against HIF-1α in comparison with control-transfected cells (Figure (Figure1F),1F), no consistent negative effect on the expression of Notch pathway components could be detected upon HIF-1α ablation or hypoxic culturing conditions. Taken together, these experiments clearly establish that Notch signaling in CCRCC cells is maintained in a HIF-1α– and HIF-2α–independent manner, irrespective of the oxygenation status of the cells.
We next sought to experimentally verify that the Notch pathway is active in CCRCC cells. Induction of Notch signaling is based on the activity of the γ-secretase complex. Chemical compounds that specifically inhibit this proteolytic activity have been extensively used for experimental studies of Notch signaling, both in vitro and in vivo (33). CCRCC cells were therefore treated with the Notch inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT), and the expression of the Notch target Hes-1 was monitored using Western blot analyses. As shown in Figure Figure2A,2A, treatment of 786-O cells with DAPT led to a prominent Hes-1 downregulation already after 8 hours, and this effect was maintained for at least 72 hours. Furthermore, treatment of 786-O cells with increasing concentrations led to a dose-dependent decrease of Hes-1 (Figure (Figure2B).2B). We could also show that treatment with the chemically distinct (33) γ-secretase inhibitor (5S)-(t-butoxycarbonylamino)-6-phenyl-(4R)hydroxy-(2R)benzylhexanoyl)-l-leu-l-phe-amide (L-685458) led to a dramatic downregulation of Hes-1 in 786-O cells (Figure (Figure2C).2C). The effect of DAPT treatment was independent of the pVHL status of the cells, as the efficacy of Hes-1 downregulation was equal in PRC3 and WT7 cells (Figure (Figure2D).2D). In order to further establish that active Notch signaling is a common feature of CCRCC cells, we assessed the effect of DAPT treatment on Hes-1 expression in the additional cell lines included in this study. The SKRC-7, SKRC-10, SKRC-21, Caki-2, and SKRC-52 cells also responded to γ-secretase treatment, albeit the extent of Hes-1 downregulation varied among the cell lines (Figure (Figure2E).2E). However, the Hes-1 protein level in SKRC-17 cells was not affected by DAPT treatment (Figure (Figure2E),2E), suggesting that this particular cell line, for unknown reasons, was insensitive to γ-secretase inhibition. Q-PCR experiments showed that the downregulation of Hes-1 in the DAPT-responsive cell lines occurred at the transcriptional level (Figure (Figure2F).2F). Together, these data show that the Notch signaling cascade is active in a wide range of CCRCC cell lines.
Prior studies have shown that active Notch signaling contributes to cellular proliferation in a distinct set of tumor cell types (20). Hence, treatment with γ-secretase inhibitors attenuates the growth capacity of these tumor cells. We therefore treated the various CCRCC cell lines with DAPT and evaluated the rates of cellular proliferation by means of [3H]thymidine incorporation. In all cell lines, [3H]thymidine incorporation was significantly reduced upon treatment with DAPT compared with vehicle control (Figure (Figure3A),3A), with the exception of the SKRC-17 cell line, which, in contrast, responded with a significant increase in proliferation upon treatment. The observation that DAPT treatment did not negatively affect proliferation of SKRC-17 indicates that the drug did not have a general toxic effect on CCRCC cells. To further exclude the possibility of nonspecific toxic effects of DAPT, 786-O cells were treated with the γ-secretase inhibitor L-685458. A significant reduction in proliferation was also noted using this inhibitor (Figure (Figure3B).3B). To further assess the effect on proliferation, we performed trypan blue (TB) exclusion experiments. As shown in Figure Figure3C3C and Supplemental Figure 1 (supplemental material available online with this article; doi:10.1172/JCI32086DS1), DAPT treatment of CCRCC cells led to a decrease in the number of viable cells detectable after 2 to 4 days in culture. In line with previous data, SKRC-17 cells were not negatively affected by γ-secretase inhibition. Since treatment with DAPT did not substantially affect the number of TB-positive cells (Figure (Figure3C)3C) compared with vehicle-treated cells, our data indicate that the decreased number of cells upon Notch inhibition was not due to increased cell death. This notion was corroborated using annexin V/propidium iodide (PI) staining (data not shown). Together, these results indicated that γ-secretase treatment was associated with a block in cell-cycle progression and not increased apoptosis. We therefore performed PI staining and flow cytometry to define the arrest pattern of DAPT-treated SKRC-52 cells. A significant increase of cells in G0G1, rising from 47% to 60%, was detected upon treatment (Figure (Figure3,3, D and E). We conclude that active Notch signaling might be important for progression beyond the G1 stage in the cell cycle. Furthermore, the sub-G1 fraction containing apoptotic or necrotic cells was not affected by DAPT treatment (Figure (Figure3E). 3E).
To further characterize the G0G1 arrest, we assayed the expression of cell-cycle regulatory factors associated with Notch signaling activity. No significant effect on the levels of c-myc, p53, Skp-2, and cyclin D1 (34–37) could be detected in CCRCC cells treated with DAPT or in siRNA transfection experiments (data not shown). We next focused on the cyclin-dependent kinase (CDK) inhibitors p21Cip1 and p27Kip1, previously linked to Notch-associated growth promotion (38, 39). Notch inhibition had no substantial effect on the expression of these cell-cycle regulators at the transcriptional level (data not shown). However, a dose- and time-dependent increase in p21Cip1 protein levels was detected when SKRC-52 cells were treated with DAPT (Figure (Figure4,4, A and B). The level of p27Kip1 was below detection in this cell line. Also, in 786-O cells, an accumulation of p21Cip1 was detected upon Notch inhibition in a time-course experiment (Figure (Figure4C).4C). A considerable accumulation of p27Kip1 protein was further detected in 786-O cells in these experiments (Figure (Figure4C).4C). SKRC-21 cells responded to DAPT treatment with an upregulation of both p21Cip1 and p27Kip1 (Figure (Figure4D).4D). In contrast, the DAPT refractory SKRC-17 cell line expressed no detectable p21Cip1, while the p27Kip1 level decreased upon treatment with the γ-secretase inhibitor (Figure (Figure4D).4D). Our findings argue that one mechanism by which Notch signaling promotes growth of CCRCC cells is by suppression of p21Cip1 and/or p27Kip1.
Our expression analyses showed that CCRCC cells express appreciable levels of Notch-1 and Notch-2 receptors, which are both sensitive to inhibition by the general pathway blocker DAPT. In addition, it remains possible that DAPT mediated its effects on CCRCC cells through γ-secretase–dependent targets other than the Notch receptors (40). We therefore targeted each of the 2 receptors using siRNA in order to elucidate their respective contribution to proliferation. 786-O, SKRC-17, and SKRC-52 cells were transfected with siRNAs directed against Notch-1 or Notch-2. The efficacy and specificity of respective siRNA was confirmed using Western blotting (Figure (Figure5A).5A). We next measured proliferation after transfection with siRNA using [3H]thymidine incorporation assays. Notch-1 ablation led to a significant decrease in proliferation compared with control siRNA in all 3 cell lines tested, including the γ-secretase–insensitive SKRC-17 cell line (Figure (Figure5B).5B). In contrast, no effect on cell proliferation could be detected in 786-0 and SKRC-52 cells upon ablation of Notch-2 expression, while SKRC-17 cells responded with increased proliferation.
We next asked whether the growth inhibitory effect of Notch-1 knockdown was associated with increased expression of p21Cip1 and/or p27Kip1 in analogy with the effects of DAPT treatment. In 786-O cells, a clear accumulation of both p21Cip1 and p27Kip1 could be detected when Notch-1 expression was ablated (Figure (Figure5C).5C). Interestingly, a considerable accumulation of p27Kip1 was detected in SKRC-17 cells transfected with siRNA against Notch-1 compared with control-transfected cells (Figure (Figure5C),5C), an experimental approach that, in contrast to treatment with DAPT, led to considerable inhibition of cell-cycle progression. Furthermore, in SKRC-52 cells transfected with siRNA against Notch-1, a substantial accumulation of p21Cip1 could be observed compared with cells transfected with control siRNA (Figure (Figure5C).5C). We also analyzed the effect of siRNA against Jagged-1 on proliferation. Western blot experiments verified the efficacy of this siRNA (Figure (Figure5D).5D). No effect on proliferation could, however, be detected upon Jagged-1 ablation (Figure (Figure5E).5E). We therefore conclude that the growth-promoting effect of Notch signaling in CCRCC cells can be specifically attributed to the Notch-1 receptor.
Our experimental data showed that the Notch cascade is expressed and active in CCRCC cell lines. This prompted us to investigate the expression of Notch pathway elements in primary CCRCCs. To show that Notch-1 is expressed in CCRCC tumor cells, we performed immunohistochemistry against Notch-1. In order to verify the specificity of the Notch-1 antibody, the staining patterns in paraffin-embedded SKRC-7 cells transfected with siRNA against Notch-1 or control siRNA were analyzed. As shown in Figure Figure6A,6A, Notch-1 staining could readily be detected in the control cells, while the staining intensity was dramatically reduced in cells transfected with siRNA against Notch-1. Six primary tumor samples were thereafter analyzed and Notch-1 expression was only detected in tumor cells, while the tumor stroma was Notch-1 negative (Figure (Figure6B).6B). In 2 of the tumors, Notch-1 staining could be detected in the nuclear region of the tumor cells, which is indicative of highly active Notch-1 signaling (Figure (Figure6B).6B). In order to more accurately quantitate Notch pathway expression in primary CCRCCs, we assessed Notch-1, Notch-2, Jagged-1, and Hes-1 levels using Western blots in a larger collection of samples, including 43 CCRCCs and 12 normal kidney extracts. The mean expression levels of all analyzed proteins were higher in tumor-derived extracts compared with normal control samples (Figure (Figure6C).6C). However, after Bonferroni’s post-hoc correction, only Notch-1 and Jagged-1 were significantly elevated in CCRCCs. To provide firmer support for the presence and function of the Notch signaling pathway in CCRCC, we isolated CCRCC cells from 2 primary tumors. Western blot experiments using extracts from one of these short-term cultures confirmed as anticipated that primary CCRCC cells expressed Notch-1, Jagged-1, and Hes-1 proteins (Figure (Figure6D).6D). When treating these cells with DAPT, a substantial decrease in Hes-1 expression could be detected, showing that the Notch pathway is constitutively active also in primary CCRCC cells (Figure (Figure6D).6D). In analogy with the biological response of established CCRCC cell lines to Notch inhibition, a decrease in proliferation could be detected in primary CCRCC cells treated with DAPT compared with control cells (Figure (Figure6E).6E). Taken together, our data indicate that the expression of Notch-1 and Jagged-1 are significantly elevated in primary CCRCCs and that inhibition of the pathway blocks growth of freshly isolated CCRCC cells.
Anchorage-independent growth represents a hallmark feature of malignant cells, and to elucidate whether Notch inhibition impaired this capacity, we performed clonogenic assays of SKRC-52 cells treated with DAPT. A remarkable effect on clonogenicity was detected, with a 70% decrease upon DAPT treatment compared with vehicle control treatment (Figure (Figure7,7, A and B).
We next wanted to clarify whether γ-secretase inhibition could restrain CCRCC growth in vivo. SKRC-52 cells were injected s.c. into nude mice, and animals were treated for 4 weeks with DAPT or vehicle control. A significant decrease in tumor growth could be detected in animals treated with DAPT in cycles with 3 days of daily injections and 4 days without treatment (Figure (Figure7C).7C). It is known that chronic treatment with γ-secretase inhibitors causes massive expansion of goblet cells in the crypt compartment due to the central role of Notch signaling in fate selection of crypt progenitor cells (41–43). We therefore also analyzed the small intestines of mice treated with intermittent DAPT dosing using immunohistochemistry. After 48 hours of treatment in the final dosing period, the villi were modestly runted and the crypt compartment was clearly elongated in DAPT-treated mice compared with control animals (Figure (Figure7D).7D). PAS staining indicated an expansion of goblet cells and an accumulation of intraluminal mucus in Notch-inhibited mice. We also noted a decreased expression of Hes-1 in the transient amplifying cell pool upon Notch inhibition. This was accompanied by a modest decrease in proliferation in treated animals compared with control animals, as indicated by decreased proliferating cell nuclear antigen (PCNA) staining (Figure (Figure7D).7D). Together, these results most likely reflect a DAPT-induced partial conversion of the proliferating precursor cell pool into postmitotic goblet cells, albeit with a much less profound phenotypic conversion compared with previously published protocols using chronic administration of γ-secretase inhibitors (42, 43). Interestingly, 96 hours after treatment, the gross morphology and expression of PCNA and Hes-1 in the transient amplifying compartment showed clear signs of recovery, though the number of goblet cells and hence mucin remained slightly elevated compared with control animals (Figure (Figure7D).7D). These results indicate that the intermittent treatment regime employed in this study would allow for a partial recovery of the small intestine between the successive rounds of drug delivery. This conclusion was substantiated by our observation that the mice maintained their weight during the course of the experiment (Supplemental Figure 2), as weight loss is a principal side effect associated with chronic treatment with γ-secretase inhibitors (43).
Further studies are, however, required to fully delineate the optimal therapeutic administration regime in order to maximize the antitumorigenic effects without interfering with the normal function of Notch signaling in regenerating tissues.
The role of Notch signaling in CCRCC has, to our knowledge, not been experimentally assessed previously, though Notch-3 and Jagged-1 mRNAs were reported to be elevated in CCRCC (44, 45). In this study, we show that the cardinal components of the Notch cascade were expressed in CCRCC cell lines. Likewise, in primary CCRCCs, we detected expression of Notch pathway proteins, with significantly higher levels of Notch-1 and Jagged-1 compared with normal kidney. Treatment of CCRCC cells with the Notch inhibitor DAPT led to a considerable decrease of Hes-1 in all but 1 cell line tested and in freshly isolated primary tumor cells, suggesting that active Notch signaling is an inherent property of CCRCC cells. Importantly, we could also show that inhibition of Notch signaling attenuates growth of CCRCC cells both in vitro and in vivo. It should be noted that the experimental data presented in this study were obtained by modulating endogenous Notch signaling, thus avoiding the pitfalls of supraphysiological levels often accomplished when exogenous icNotch is introduced. For example, in CNS stem cells, low levels of icNotch-1 promote growth whereas high levels induce growth arrest (46).
γ-Secretase inhibitors are valuable tools for delineating the cell biological function of the Notch cascade, but since they affect all Notch receptor paralogs, our experiments did not specify the individual contributions of the respective receptors. Furthermore, γ-secretase might affect other proteins involved in proliferation control (40). However, since ablation of Notch-1 using siRNA led to decreased proliferation, we conclude that this Notch receptor is the critical target for the antiproliferative effect of γ-secretase inhibition in CCRCC cells. When targeting Jagged-1, no effect on proliferation could be detected, indicating that Notch receptor activation in vitro is not a consequence of autocrine or paracrine activation of the Notch-1 receptor by Jagged-1. However, in primary tumor specimens, Jagged-1 expression was significantly elevated compared with normal kidney, implying a potential involvement in other aspects of tumorigenic growth. For example, in head and neck squamous carcinoma, elevated Jagged-1 expression, as a consequence of RAS/MAPK activation, was shown to activate Notch receptors on tumor-infiltrating endothelial cells and thereby promote angiogenesis (47). Interestingly, the SKRC-17 cells that were refractory to γ-secretase treatment displayed a robust decline in proliferation upon ablation of Notch-1 expression. How these cells escape inhibition of γ-secretase cleavage remains to be determined. It should be noted that some T cell acute lymphoblastic leukemia cells harboring Notch-1–activating mutations also were refractory to γ-secretase treatment (18). Recent data show that mutations of FBW7, a ubiquitin ligase involved in degradation of icNotch, render these cells refractory to pharmacological inhibitors (48). It will be important to clarify whether mutations in the Notch pathway are present in a subset of CCRCCs.
The regulatory effect of Notch signaling on p21Cip1 and p27Kip1, 2 CDK inhibitory proteins of pivotal importance in cell-cycle control, seems to be one important determinant for the cell type–specific effects of Notch signaling. In cell types in which Notch signaling is growth inhibiting, such as keratinocytes and small cell lung cancer cells, induced Notch signaling leads to upregulation of p21Cip1 and/or p27Kip1 (49, 50). In other cell types, such as endothelial and pancreatic cancer cells, high Notch activity is associated with decreased expression of p21Cip1 and/or p27Kip1 (38, 39). Our data suggest that elevation of p21Cip1 and p27Kip1 might represent a potential mechanism for the growth-restraining effect of Notch inhibition in CCRCC cells. It is noteworthy that in CCRCC, low p27Kip1 expression has been associated with unfavorable prognosis (51, 52). Further studies are required to determine the molecular link between p21Cip1 and p27Kip1 regulation and Notch signaling in CCRCC cells.
Due to the loss of VHL and hence constitutive activation of HIF-1α and, in particular, HIF-2α, CCRCC tumors are characterized by an oxygen-independent hypoxic response. The loss of VHL is an early event in the genesis of CCRCC and is considered to be associated with a gatekeeper function of the tumor suppressor gene, i.e., VHL loss is a prerequisite for tumor formation, but additional oncogenic events affecting other aspects of the tumorigenic process are most likely involved in tumor progression (12). We have previously reported that Notch signaling is elevated in hypoxic human neuroblastoma cells (27, 28). Recently, it was shown that differentiation of neuronal and muscle progenitor cells was inhibited by hypoxia in a Notch signaling–dependent manner, and a physical interaction between HIF-1α and icNotch-1, which potentiated activation of Notch target genes, was reported (26). Since our data clearly showed that Notch signaling activity in CCRCC cells was not suppressed by pVHL restoration or HIF-α knockdown and not enhanced by hypoxia, we consider it unlikely that the VHL/HIF pathway augments Notch signaling in CCRCC cells. On the contrary, a slight increase in primary downstream target genes could, for unknown reasons, be detected in the pVHL-reconstituted CCRCC cells. However, since an almost complete downregulation of Hes-1 could be detected upon γ-secretase treatment irrespective of the pVHL status of the cells, we conclude that the γ-secretase responsiveness is not associated with the VHL/HIF axis.
Until recently, no efficient treatment for metastatic CCRCC was available. However, several kinase inhibitors, e.g., sorafenib and sunitinib, show substantial effects on progression-free survival for patients with adverse disease (2, 3). The efficacy of these drugs most likely relates to their capacity to inhibit HIF-mediated autocrine growth factor signaling and proangiogenic effects. Interestingly, loss of VHL is associated with good prognosis in CCRCC (53, 54). The therapeutic effect of γ-secretase inhibition on CCRCC tumor growth indicates that inhibition of Notch signaling might represent a complementary therapeutic approach for treatment of CCRCC. However, it is well known that in vivo use of γ-secretase inhibitors is associated with considerable adverse effects (43, 55). In particular, intestinal differentiation is perturbed due to massive expansion of goblet cells (41, 42). Our intermittent administration regime decreased the adverse effects on the rapidly turned over crypt cells while the cytostatic effect on the tumors was maintained. A comprehensive evaluation of the optimal administration regime of γ-secretase inhibitors is therefore of high priority. It should be noted that histopathological analyses revealed no adverse effects on normal kidney in long-term treatment of mice with γ-secretase inhibitors in a previous study (43). In addition, we noticed a striking inhibition of clonogenicity in soft agar experiments when CCRCC cells were treated with DAPT. It will be important to clarify whether the general effects on proliferation might be associated with a depletion of tumor-initiating cells, an effect of Notch inhibition that has been observed in other tumors, such as medulloblastoma (56).
Several recent studies unequivocally show that Notch signaling is pivotal for tumor angiogenesis (57–60). The Notch ligand Dll-4 seems to be essential for tumor angiogenesis, and thus, Dll-4 inhibition is emerging as a promising antiangiogenic therapeutic approach. Importantly, the expression of Dll-4 is particularly high in endothelial cells in the richly vascularized CCRCC tumors (38). However, based on the results presented in this study, we speculate that global targeting of the Notch pathway in CCRCC might be particularly efficient, since it might serve a dual purpose by affecting the growth capacity of the tumor cells and at the same time impeding angiogenesis.
The CCRCC cell line 786-O was obtained from ATCC and maintained in DMEM (Invitrogen) supplemented with 10% FCS and 1% penicillin/streptomycin (PEST). 786-O subclones stably transfected with either pRc/CMV (PRC3) or pRc/CMV-HA-VHL (WT7) were generous gifts from W.G. Kaelin Jr. (Dana-Farber Cancer Institute and Brigham and Women’s Hospital, Harvard Medical School, Boston, USA) (4) and were maintained as above with the addition of 1 mg/ml G418 (GIBCO; Invitrogen). The CCRCC cell lines SKRC-7, SKRC-10, SKRC-17, and SKRC-21 (29, 31) were kindly provided by E. Oosterwijk (Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands) and maintained in RPMI 1640 (Invitrogen) containing 10% FCS and PEST. The CCRCC cell lines SKRC-52 and Caki-2 were maintained in RPMI 1640 as above. The primary tumor cells (PT I and PT II) were derived from primary lesions provided with informed consent by 2 patients who had undergone radical nephrectomy at University Hospital MAS. The primary lesions were histopathologically verified as CCRCCs. After surgery, tumor tissue fragments were processed by enzymatic digestion, and tumor cells were maintained in high-glucose DMEM (Invitrogen) supplemented with 10% FCS and PEST. Cells were treated with indicated concentrations of the γ-secretase inhibitor DAPT (Calbiochem) or 5 μM L-685458 (Bachem) or the corresponding volume of the vehicle DMSO for indicated times.
Cells were seeded in 60-mm plates 24 hours prior to siRNA transfection. Cells were then transfected with control siRNA or siRNA against HIF-1α or HIF-2α (Ambion) or siRNA against Notch-1, Notch-2, or Jagged-1 (Santa Cruz Biotechnology Inc.) using Lipofectamine 2000 (Invitrogen) for 6 or 24 hours in OptiMEM I Reduced Serum Medium (Invitrogen). Cells were harvested at indicated time points. In hypoxia experiments, cells were grown at 1% O2 in a 400 Hypoxia Workstation (Ruskinn Technology Ltd.) connected to a Ruskinn gas mixer module.
Cells were lysed in RIPA buffer, separated on an SDS-PAGE gel, and blotted onto Immobilon-P (Millipore) or Hybond-C (Amersham) membranes. Antibodies are provided in Supplemental Methods. Western blot experiments were performed at least 3 times.
Total RNA extraction and quantification of gene expression using SYBR Green (Applied Biosystems) were performed as described previously (61). The relative quantification of mRNA was done using the comparative Ct method and normalized to 3 endogenous reference genes, succinate dehydrogenase complex subunit A (SDHA), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHAZ), and ubiquitin C (UBC) (62). Primer sequences are given in Supplemental Table 1. The experiments were repeated twice and the data shown as mean + SD of representative experiments, performed in triplicate.
In γ-secretase inhibition experiments, 5.0 × 104 cells were seeded in 96-well plates in 200 μl 1% FCS–supplemented media or 1% FCS media supplemented with DMSO or DAPT and incubated for 72 hours. [3H]thymidine (Amersham Life Sciences) was then added to the culture. Cells were harvested after 24 hours, and the incorporated [3H]thymidine was measured as counts per minute in a β-liquid scintillation counter (LKB RackBeta Wallace). Each experiment was performed 6 times and repeated 3 times. In siRNA experiments, cells were seeded in 60-mm plates 24 hours prior to transfection. The cells were then transfected with siRNA as indicated above, after which 5.0 × 104 cells were reseeded in 96-well plates in 200 μl 1% FCS–supplemented media and incubated for 72 hours whereafter [3H]thymidine incorporation was assessed as above.
7.0 × 104 cells were seeded in 60-mm plates 1 day prior to initiation of experiment. Starting at day 0, cells were trypsinized and stained with 0.4% TB solution (Sigma-Aldrich), and both viable and dead cells were counted in a Bürker chamber. Every other day, starting at day 0, cultures for subsequent counting were supplemented with fresh media containing 1% FCS, PEST, and DMSO or DAPT. Each experiment was performed in triplicate and repeated twice.
For cell-cycle distribution experiments, cells were synchronized with serum-free medium for 24 hours and then supplemented with fresh medium containing 1% FCS, PEST, and DMSO or DAPT for 24 hours. Cells were harvested by centrifugation, resuspended in 70% ethanol, and stored at –20°C. Cells were washed in cold PBS, after which 800 μl Vindelöv solution was added to the cells and left to incubate for 20 minutes on ice. DNA analyses were performed using a FACSCalibur flow cytometer (BD), and the fraction of sub-G1, G0G1, S, and G2M cells was determined using CellQuest 3.2 software (BD). Each experiment was performed in triplicate and repeated 3 times.
Tumor samples collected at the University Hospital in Umeå, Sweden, including 6 nephrectomy specimens from 6 patients, were analyzed by immunohistochemistry. The tumors were classified as CCRCCs according to the Heidelberg classification system (63). All tumor samples were obtained after permission from the patients with informed and signed consent, and the study was approved by the Institutional Review Board. Paraffin sections (4 μm) from the paraffin-embedded tissue specimens were deparaffinized and microwave treated according to standard procedures. After antigen retrieval, Notch-1 (Santa Cruz Biotechnology Inc.) immunoreactivity was detected using the Dako EnVision system and Dako TechMate 500. Sections were counterstained with H&E. To evaluate antibody specificity, Notch-1 immunoreactivity of siRNA control-transfected SKRC-7 cells or SKRC-7 cells in which the antigen had been eliminated by siRNA against Notch-1 was performed. Immunohistochemistry of SKRC-7 cells were performed as described above.
Preparation of protein extracts from fresh-frozen tissues was performed as described earlier (51). Protein extract from each sample and a positive control (SKRC-52) were separated on SDS-PAGE, and immunoblotting was performed as described above. The amount of protein in each sample was determined by densitometry (ImageJ software, v. 1.34) and normalized to the amount of actin and positive control.
1.5 × 104 SKRC-52 cells were resuspended in 1% FCS RPMI 1640 medium containing 0.7% agarose and DMSO or DAPT. This suspension was layered over a 0.5% agar medium base layer in 60-mm plates and treated with DMSO or DAPT every second day for a total of 30 days, whereafter macroscopically visible colonies were counted. Each experiment was performed in triplicate and repeated 3 times.
2.0 × 106 SKRC-52 cells in 100 μl of PBS were injected s.c. into the flank of 6- to 8-week-old athymic female mice (NMRI strain nu/nu; Taconic). Tumor volume was determined as Àls2/6, where l = long side and s = short side. When the tumor volumes reached approximately 100 mm3, mice were treated s.c. with 100 μl vehicle control (10% ethanol, 90% corn oil) or 10 mg/kg DAPT (dissolved in 10% ethanol, 90% corn oil) as previously described (57). A regime of daily treatment was continued for 3 days followed by 4 days without treatment. This treatment regime was repeated 5 times. At the end of the dosing period, animals were sacrificed and small intestines of 3 DAPT- and 3 vehicle-treated mice were collected for histological analyses. Samples were also collected from 3 DAPT-treated mice 4 days after the final dosing. Formalin-fixed tissues were prepared as described above. Sections were stained with PAS in order to visualize carbohydrate-rich mucin. Immunoreactivity was analyzed as above using a PCNA antibody (Dako) or Hes-1 antiserum kindly provided by T. Sudo (Toray Industries Inc., Kamakura, Japan). All animal experiments were approved by the Malmö/Lund ethical committee, Lund, Sweden (approval no. M24–07).
Statistical analyses were performed using 2-tailed Student’s t test. Bonferroni’s correction was used to adjust for multiple comparisons in analyses of primary tumor samples.
We are grateful to W.G. Kaelin Jr. for providing the PRC3 and WT7 cells; E. Oosterwijk for providing SKRC-7, SKRC-10, SKRC-17, and SKRC-21 cells; and T. Sudo for generously providing the Hes-1 antiserum. We are also grateful to M. Hellström for advice regarding the preparation of DAPT for the in vivo experiments and to E. Fredlund for statistical advice. This work was supported by grants from the Swedish Cancer Society, the Children’s Cancer Foundation of Sweden, υke Wiberg’s Foundation, Ollie and Elof Ericsson’s Foundation, the Crafoord Foundation, Gunnar Nilsson’s Cancer Foundation, and Malmö University Hospital research funds.
Nonstandard abbreviations used: CCRCC, clear cell renal cell carcinoma; CDK, cyclin-dependent kinase; DAPT, N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester; Dll, Delta-like ligand; Hes, hairy and enhancer of split; Hey, Hes-related repressor protein; icNotch, intracellular Notch; L-685458, (5S)-(t-butoxycarbonylamino)-6-phenyl-(4R)hydroxy-(2R)benzylhexanoyl)-l-leu-l-phe-amide; PCNA, proliferating cell nuclear antigen; PEST, penicillin/streptomycin; PI, propidium iodide; pVHL, VHL protein; Q-PCR, quantitative real-time PCR; RCC, renal cell carcinoma; TB, trypan blue; VHL, von Hippel–Lindau.
Conflict of interest: The authors have declared that no conflict of interest exists.
Citation for this article: J. Clin. Invest. 118:217–228 (2008). doi:10.1172/JCI32086