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J Cell Biol. 1996 May 1; 133(3): 667–681.
PMCID: PMC2120827

Identification of two Sox17 messenger RNA isoforms, with and without the high mobility group box region, and their differential expression in mouse spermatogenesis


The different mRNA isoforms of the mouse Sox17 gene were isolated from adult mouse testis cDNAs. One form (referred to as form Sox17) encodes an Sry-related protein of 419 amino acids containing a single high mobility group box near the NH2-terminus, while the other form (referred to as form t-Sox17) shows a unique mRNA isoform of the Sox17 gene with a partial deletion of the HMG box region. Analysis of genomic DNA revealed that these two isoforms were produced at least by alternative splicing of the exon corresponding to the 5' untranslated region and NH2-terminal 102 amino acids. RNA analyses in the testis revealed that form Sox17 began at the pachytene spermatocyte stage and was highly accumulated in round spermatids. Protein analyses revealed that t-Sox17 isoforms, as well as Sox17 isoforms, were translated into the protein products in the testis, although the amount of t-Sox17 products is lower in comparison to the high accumulation of t-Sox17 mRNA. By the electrophoretic mobility-shift assay and the random selection assay using recombinant Sox17 and t-Sox17 proteins, Sox17 protein is a DNA-binding protein with a similar sequence specificity to Sry and the other members of Sox family proteins, while t-Sox17 shows no apparent DNA-binding activity. Moreover, by a cotransfection experiment using a luciferase reporter gene, Sox17 could stimulate transcription through its binding site, but t-Sox17 had little effect on reporter gene expression. Thus, these findings suggest that Sox17 may function as a transcriptional activator in the premeiotic germ cells, and that a splicing switch into t-Sox17 may lead to the loss of its function in the postmeiotic germ cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bunker CA, Kingston RE. Identification of a cDNA for SSRP1, an HMG-box protein, by interaction with the c-Myc oncoprotein in a novel bacterial expression screen. Nucleic Acids Res. 1995 Jan 25;23(2):269–276. [PMC free article] [PubMed]
  • Capel B, Swain A, Nicolis S, Hacker A, Walter M, Koopman P, Goodfellow P, Lovell-Badge R. Circular transcripts of the testis-determining gene Sry in adult mouse testis. Cell. 1993 Jun 4;73(5):1019–1030. [PubMed]
  • Chardard D, Chesnel A, Gozé C, Dournon C, Berta P. Pw Sox-1: the first member of the Sox gene family in Urodeles. Nucleic Acids Res. 1993 Jul 25;21(15):3576–3576. [PMC free article] [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Clépet C, Schafer AJ, Sinclair AH, Palmer MS, Lovell-Badge R, Goodfellow PN. The human SRY transcript. Hum Mol Genet. 1993 Dec;2(12):2007–2012. [PubMed]
  • Connor F, Wright E, Denny P, Koopman P, Ashworth A. The Sry-related HMG box-containing gene Sox6 is expressed in the adult testis and developing nervous system of the mouse. Nucleic Acids Res. 1995 Sep 11;23(17):3365–3372. [PMC free article] [PubMed]
  • Denny P, Swift S, Brand N, Dabhade N, Barton P, Ashworth A. A conserved family of genes related to the testis determining gene, SRY. Nucleic Acids Res. 1992 Jun 11;20(11):2887–2887. [PMC free article] [PubMed]
  • Denny P, Swift S, Connor F, Ashworth A. An SRY-related gene expressed during spermatogenesis in the mouse encodes a sequence-specific DNA-binding protein. EMBO J. 1992 Oct;11(10):3705–3712. [PubMed]
  • Dunn TL, Mynett-Johnson L, Wright EM, Hosking BM, Koopman PA, Muscat GE. Sequence and expression of Sox-18 encoding a new HMG-box transcription factor. Gene. 1995 Aug 19;161(2):223–225. [PubMed]
  • Erickson RP. Post-meiotic gene expression. Trends Genet. 1990 Aug;6(8):264–269. [PubMed]
  • Foster JW, Dominguez-Steglich MA, Guioli S, Kwok C, Weller PA, Stevanović M, Weissenbach J, Mansour S, Young ID, Goodfellow PN, et al. Campomelic dysplasia and autosomal sex reversal caused by mutations in an SRY-related gene. Nature. 1994 Dec 8;372(6506):525–530. [PubMed]
  • Foulkes NS, Mellström B, Benusiglio E, Sassone-Corsi P. Developmental switch of CREM function during spermatogenesis: from antagonist to activator. Nature. 1992 Jan 2;355(6355):80–84. [PubMed]
  • Fujita T, Nolan GP, Ghosh S, Baltimore D. Independent modes of transcriptional activation by the p50 and p65 subunits of NF-kappa B. Genes Dev. 1992 May;6(5):775–787. [PubMed]
  • Gozé C, Poulat F, Berta P. Partial cloning of SOX-11 and SOX-12, two new human SOX genes. Nucleic Acids Res. 1993 Jun 25;21(12):2943–2943. [PMC free article] [PubMed]
  • Gubbay J, Collignon J, Koopman P, Capel B, Economou A, Münsterberg A, Vivian N, Goodfellow P, Lovell-Badge R. A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature. 1990 Jul 19;346(6281):245–250. [PubMed]
  • Harley VR, Jackson DI, Hextall PJ, Hawkins JR, Berkovitz GD, Sockanathan S, Lovell-Badge R, Goodfellow PN. DNA binding activity of recombinant SRY from normal males and XY females. Science. 1992 Jan 24;255(5043):453–456. [PubMed]
  • Harley VR, Lovell-Badge R, Goodfellow PN. Definition of a consensus DNA binding site for SRY. Nucleic Acids Res. 1994 Apr 25;22(8):1500–1501. [PMC free article] [PubMed]
  • Jantzen HM, Admon A, Bell SP, Tjian R. Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins. Nature. 1990 Apr 26;344(6269):830–836. [PubMed]
  • Jantzen HM, Chow AM, King DS, Tjian R. Multiple domains of the RNA polymerase I activator hUBF interact with the TATA-binding protein complex hSL1 to mediate transcription. Genes Dev. 1992 Oct;6(10):1950–1963. [PubMed]
  • Jay P, Gozé C, Marsollier C, Taviaux S, Hardelin JP, Koopman P, Berta P. The human SOX11 gene: cloning, chromosomal assignment and tissue expression. Genomics. 1995 Sep 20;29(2):541–545. [PubMed]
  • Kamachi Y, Sockanathan S, Liu Q, Breitman M, Lovell-Badge R, Kondoh H. Involvement of SOX proteins in lens-specific activation of crystallin genes. EMBO J. 1995 Jul 17;14(14):3510–3519. [PubMed]
  • Koopman P, Gubbay J, Vivian N, Goodfellow P, Lovell-Badge R. Male development of chromosomally female mice transgenic for Sry. Nature. 1991 May 9;351(6322):117–121. [PubMed]
  • Noce T, Fujiwara Y, Ito M, Takeuchi T, Hashimoto N, Yamanouchi M, Higashinakagawa T, Fujimoto H. A novel murine zinc finger gene mapped within the tw18 deletion region expresses in germ cells and embryonic nervous system. Dev Biol. 1993 Feb;155(2):409–422. [PubMed]
  • Noce T, Fujiwara Y, Sezaki M, Fujimoto H, Higashinakagawa T. Expression of a mouse zinc finger protein gene in both spermatocytes and oocytes during meiosis. Dev Biol. 1992 Oct;153(2):356–367. [PubMed]
  • Poulat F, Girard F, Chevron MP, Gozé C, Rebillard X, Calas B, Lamb N, Berta P. Nuclear localization of the testis determining gene product SRY. J Cell Biol. 1995 Mar;128(5):737–748. [PMC free article] [PubMed]
  • Rossi P, Dolci S, Albanesi C, Grimaldi P, Geremia R. Direct evidence that the mouse sex-determining gene Sry is expressed in the somatic cells of male fetal gonads and in the germ cell line in the adult testis. Mol Reprod Dev. 1993 Apr;34(4):369–373. [PubMed]
  • Ruppert S, Cole TJ, Boshart M, Schmid E, Schütz G. Multiple mRNA isoforms of the transcription activator protein CREB: generation by alternative splicing and specific expression in primary spermatocytes. EMBO J. 1992 Apr;11(4):1503–1512. [PubMed]
  • Saido TC, Iwatsubo T, Mann DM, Shimada H, Ihara Y, Kawashima S. Dominant and differential deposition of distinct beta-amyloid peptide species, A beta N3(pE), in senile plaques. Neuron. 1995 Feb;14(2):457–466. [PubMed]
  • Saido TC, Nagao S, Shiramine M, Tsukaguchi M, Sorimachi H, Murofushi H, Tsuchiya T, Ito H, Suzuki K. Autolytic transition of mu-calpain upon activation as resolved by antibodies distinguishing between the pre- and post-autolysis forms. J Biochem. 1992 Jan;111(1):81–86. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Schilham MW, van Eijk M, van de Wetering M, Clevers HC. The murine Sox-4 protein is encoded on a single exon. Nucleic Acids Res. 1993 Apr 25;21(8):2009–2009. [PMC free article] [PubMed]
  • Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ, Foster JW, Frischauf AM, Lovell-Badge R, Goodfellow PN. A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif. Nature. 1990 Jul 19;346(6281):240–244. [PubMed]
  • Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. [PubMed]
  • Stevanović M, Lovell-Badge R, Collignon J, Goodfellow PN. SOX3 is an X-linked gene related to SRY. Hum Mol Genet. 1993 Dec;2(12):2013–2018. [PubMed]
  • Takamatsu N, Kanda H, Tsuchiya I, Yamada S, Ito M, Kabeno S, Shiba T, Yamashita S. A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein. Mol Cell Biol. 1995 Jul;15(7):3759–3766. [PMC free article] [PubMed]
  • Uwanogho D, Rex M, Cartwright EJ, Pearl G, Healy C, Scotting PJ, Sharpe PT. Embryonic expression of the chicken Sox2, Sox3 and Sox11 genes suggests an interactive role in neuronal development. Mech Dev. 1995 Jan;49(1-2):23–36. [PubMed]
  • van de Wetering M, Oosterwegel M, Dooijes D, Clevers H. Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box. EMBO J. 1991 Jan;10(1):123–132. [PubMed]
  • van de Wetering M, Oosterwegel M, van Norren K, Clevers H. Sox-4, an Sry-like HMG box protein, is a transcriptional activator in lymphocytes. EMBO J. 1993 Oct;12(10):3847–3854. [PubMed]
  • Waeber G, Meyer TE, LeSieur M, Hermann HL, Gérard N, Habener JF. Developmental stage-specific expression of cyclic adenosine 3',5'-monophosphate response element-binding protein CREB during spermatogenesis involves alternative exon splicing. Mol Endocrinol. 1991 Oct;5(10):1418–1430. [PubMed]
  • Wagner T, Wirth J, Meyer J, Zabel B, Held M, Zimmer J, Pasantes J, Bricarelli FD, Keutel J, Hustert E, et al. Autosomal sex reversal and campomelic dysplasia are caused by mutations in and around the SRY-related gene SOX9. Cell. 1994 Dec 16;79(6):1111–1120. [PubMed]
  • Wilson D, Sheng G, Lecuit T, Dostatni N, Desplan C. Cooperative dimerization of paired class homeo domains on DNA. Genes Dev. 1993 Nov;7(11):2120–2134. [PubMed]
  • Wright E, Hargrave MR, Christiansen J, Cooper L, Kun J, Evans T, Gangadharan U, Greenfield A, Koopman P. The Sry-related gene Sox9 is expressed during chondrogenesis in mouse embryos. Nat Genet. 1995 Jan;9(1):15–20. [PubMed]
  • Wright EM, Snopek B, Koopman P. Seven new members of the Sox gene family expressed during mouse development. Nucleic Acids Res. 1993 Feb 11;21(3):744–744. [PMC free article] [PubMed]

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