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J Bacteriol. 1987 April; 169(4): 1441–1446.
PMCID: PMC211965

Lipopolysaccharides of Pseudomonas spp. that stimulate plant growth: composition and use for strain identification.


The outer membrane proteins of a series of fluorescent, root-colonizing, plant-growth-stimulating Pseudomonas spp. having been characterized (L. A. de Weger et al., J. Bacteriol. 165:585-594, 1986), the lipopolysaccharides (LPSs) of these strains were examined. The chemical composition of the LPSs of the three best-studied plant-growth-stimulating Pseudomonas strains WCS358, WCS361, and WCS374 and of P. aeruginosa PAO1 as a reference strain was determined and appeared to differ from strain to strain. The 2,6-dideoxy-2-aminosugar quinovasamine was the most abundant compound in the LPS of strain WCS358. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified LPS and of proteinase K-treated cell envelopes revealed ladderlike patterns for most of these strains. These patterns were not substantially influenced by differences in culture conditions. Analysis of proteinase K-treated cell envelopes of 24 root-colonizing Pseudomonas spp. revealed a unique band pattern for each strain, suggesting a great variety in the LPS structures present in these root colonizers. Therefore, electrophoretic analysis of LPS can be used for characterization and identification of the fluorescent root-colonizing Pseudomonas strains.

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Selected References

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  • AMES BN, DUBIN DT. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed]
  • Anderson AJ. Unique aspects of the cell surface polysaccharide of Pseudomonas phaseolicola as demonstrated by bacteriophage specificity. Can J Microbiol. 1980 Dec;26(12):1422–1427. [PubMed]
  • Anderson AJ. Differences between lipopolysaccharide compositions of plant pathogenic and saprophytic pseudomonas species. Appl Environ Microbiol. 1984 Jul;48(1):31–35. [PMC free article] [PubMed]
  • Darveau RP, Hancock RE. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol. 1983 Aug;155(2):831–838. [PMC free article] [PubMed]
  • de Weger LA, van Boxtel R, van der Burg B, Gruters RA, Geels FP, Schippers B, Lugtenberg B. Siderophores and outer membrane proteins of antagonistic, plant-growth-stimulating, root-colonizing Pseudomonas spp. J Bacteriol. 1986 Feb;165(2):585–594. [PMC free article] [PubMed]
  • Goldman RC, Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem. 1980;107(1):145–153. [PubMed]
  • Graham TL, Sequeira L, Huang TS. Bacterial lipopolysaccharides as inducers of disease resistance in tobacco. Appl Environ Microbiol. 1977 Oct;34(4):424–432. [PMC free article] [PubMed]
  • Hackstadt T, Peacock MG, Hitchcock PJ, Cole RL. Lipopolysaccharide variation in Coxiella burnetti: intrastrain heterogeneity in structure and antigenicity. Infect Immun. 1985 May;48(2):359–365. [PMC free article] [PubMed]
  • Hitchcock PJ, Brown TM. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. [PMC free article] [PubMed]
  • Jann B, Jann K, Beyaert GO. 2-Amino-2,6-dideoxy-d-glucose (D-quinovosamine): a constituent of the lipopolysaccharides of Vibrio cholerae. Eur J Biochem. 1973 Sep 3;37(3):531–534. [PubMed]
  • KING EO, WARD MK, RANEY DE. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed]
  • Kropinski AM, Chan LC, Milazzo FH. The extraction and analysis of lipopolysaccharides from Pseudomonas aeruginosa strain PAO, and three rough mutants. Can J Microbiol. 1979 Mar;25(3):390–398. [PubMed]
  • Kropinski AM, Kuzio J, Angus BL, Hancock RE. Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1982 Feb;21(2):310–319. [PMC free article] [PubMed]
  • Lugtenberg B, Meijers J, Peters R, van der Hoek P, van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. [PubMed]
  • Marugg JD, van Spanje M, Hoekstra WP, Schippers B, Weisbeek PJ. Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358. J Bacteriol. 1985 Nov;164(2):563–570. [PMC free article] [PubMed]
  • Palva ET, Mäkelä PH. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Eur J Biochem. 1980;107(1):137–143. [PubMed]
  • Partridge SM. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. [PubMed]
  • Tsai CM, Frasch CE. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. [PubMed]
  • VOGEL HJ, BONNER DM. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed]
  • Whatley MH, Bodwin JS, Lippincott BB, Lippincott JA. Role of Agrobacterium cell envelope lipopolysaccharide in infection site attachment. Infect Immun. 1976 Apr;13(4):1080–1083. [PMC free article] [PubMed]
  • Wright BG, Rebers PA. Procedure for determining heptose and hexose in lipopolysaccharides. Modification of the cysteine-sulfuric acid method. Anal Biochem. 1972 Oct;49(2):307–319. [PubMed]

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