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Microsomal triglyceride transfer protein (MTP) is an endoplasmic reticulum (ER)–resident lipid transfer protein involved in the biosynthesis and lipid loading of apolipoprotein B. MTP was recently suggested to directly regulate the biosynthesis of the MHC I–like, lipid antigen presenting molecule CD1d, based on coprecipitation experiments and lipid loading assays. However, we found that the major impact of MTP deficiency occurred distal to the ER and Golgi compartments. Thus, although the rates of CD1d biosynthesis, glycosylation maturation, and internalization from the cell surface were preserved, the late but essential stage of recycling from lysosome to plasma membrane was profoundly impaired. Likewise, functional experiments indicated defects of CD1d-mediated lipid presentation in the lysosome but not in the secretory pathway. These intriguing findings suggest a novel, unexpected role of MTP at a late stage of CD1d trafficking in the lysosomal compartment.
The CD1 family of glycoproteins is composed of conserved MHC-like, β2-microglobulin–associated glycoproteins that specialize in the capture of self and microbial lipid antigens for presentation to T cells (1, 2). Recent studies have revealed that CD1-mediated antigen presentation depended on a set of proteins involved in general lipid metabolism. Thus, lysosomal saposins, which promote the enzymatic degradation of glycosphingolipids (GSLs), also performed essential lipid exchange reactions between membranes and CD1 proteins (3–5). CD1e, a lysosomal member of the human CD1 family, enhanced lipid processing by degrading enzymes (6). Serum VLDL and the surface LDL receptor directly contributed to the transport and uptake of exogenous lipids (7). Disruption of NPC1, a transmembrane protein present in late endosomal membranes, interrupted GSL trafficking from late endosome to lysosome and impaired CD1-mediated antigen presentation and NKT cell development (8).
Several lines of evidence also suggest a role for microsomal triglyceride transfer protein (MTP), an ER-resident protein that functions as a lipid transfer protein and is essential for the loading of apolipoprotein B (apoB) with cholesterol, triacylglycerol, and phospholipids (9). MTP was originally characterized as a heterodimer of protein disulfide isomerase and a 97-kD subunit in the ER of hepatocytes and enterocytes (10–12), but recent studies have demonstrated weak expression in hemopoietic cells as well, including T cells and dendritic cells (13). Because MTP coprecipitated with CD1d and could transfer lipids onto plate-bound CD1d in a cell-free assay, it was proposed that MTP might assist in loading lipids onto CD1 molecules during biosynthesis in the ER in a manner similar to chaperone-assisted loading of peptides onto nascent MHC class I molecules (13, 14). In the absence of MTP, misfolded CD1d molecules would be retained in the ER, explaining the reduction of surface CD1d and the impaired antigen presentation observed in MTP-deficient cells. Consistent with this hypothesis, MTP ablation after injection of double-stranded RNA (dsRNA [polyI:C]) in mttpfl/fl Mx1-Cre mice afforded resistance to diseases mediated by CD1d-restricted NKT cells such as αGalCer-induced hepatitis and oxazolone-induced colitis (14).
Here, we have examined the presentation of lipid antigens and studied the dynamics of the cellular trafficking of CD1d and lipids in cells lacking MTP. Severe defects in lipid antigen presentation were observed, but, surprisingly, they appeared to be selective for lipid antigens requiring lysosomal processing or loading rather than those acquired in the secretory pathway. In addition, cell biological assays revealed that MTP deficiency selectively impaired CD1d trafficking between the lysosome and the plasma membrane, far from the proposed site of action during biosynthesis in the ER. Consistent with these findings, the development of Vα14-Jα18 NKT cells, which requires lysosomal loading of natural ligands, was partially impaired in radiation chimeras reconstituted with MTP-deficient bone marrow cells. These intriguing findings converge to define a novel MTP-regulated mechanism that controls an essential step in the presentation of many lipid antigens, the recycling of CD1d from the lysosome to the plasma membrane.
Because expression of CD1d by cortical thymocytes is essential for the development of Vα14 NKT cell, we crossed mttpfl/fl mice to mice expressing the pLck-Cre transgene expressed in thymocytes. Genomic typing of WT and mttpfl/fl mice is shown in Fig. 1 A. Despite ~95% genetic ablation of the floxed mttp gene segment (Fig. 1 B), the frequencies of Vα14 NKT cells in thymus and spleen were not significantly diminished (Fig. 1 C). As an assay to probe for NKT ligand expression by thymocytes, we measured IL-2 release after exposure of NKT hybridomas to MTP-deficient thymocytes. Despite the absence of NKT cells' developmental defect, the response of the Vα14 hybridoma DN32.D3 was reduced, whereas, in contrast, the non-Vα14 hybridoma TCB11 was unaffected (Fig. 1 D). These hybridomas are widely used to probe for endogenous ligands acquired in the lysosomal versus the secretory pathway, respectively. Thus, DN32.D3 responds to iGb3 loaded onto CD1d by saposins in the lysosome, whereas TCB11 responds to an unidentified ligand loaded in the secretory pathway. Because the ablation of mttp was incomplete and low residual ligand expression could explain conserved NKT cell development in vivo, we crossed mttpfl/fl mice to mice expressing the Cre recombinase under control of the IFN-inducible Mx1 promoter. Bone marrow cells from mttpfl/fl Mx1-Cre (fl/fl-Mx1Cre) and WT (nonfloxed mttp) littermates treated with multiple injections of dsRNA (polyI:C) were used to reconstitute lethally irradiated CD1d−/− hosts. This procedure achieved 99.6% deletion of mttp in the bone marrow (Fig. 2 A). In this system, Vα14 NKT cells were modestly reduced by 50–60% both in the thymus and the spleen, whereas B cells and CD4 and CD8 T cells were preserved (Fig. 2, B and C). To test whether the requirement of MTP was intrinsic to the developing NKT cells or to the CD1d-presenting cells, we generated mixed bone marrow chimeras by mixing bone marrow from Ly5.1 WT mice and from dsRNA (polyI:C)–injected fl/fl-Mx1Cre mice (Ly5.2). Although the overall hemopoietic reconstitution by MTP-deficient bone marrow cells was less efficient than WT, NKT cell development was unaffected relative to other lymphocyte compartments, including CD4 and CD8 T cells and B cells (Fig. 2 D). Considering the conserved level of CD1d expression (Fig. 2 E), these results are consistent with a selective defect in lipid ligand presentation by CD1d-expressing thymocytes as a mechanism for the decreased frequency of NKT cells.
To investigate the functional and cell biological defects of CD1d-mediated lipid antigen presentation in cells lacking MTP, we used small interfering RNA (siRNA) to knock down (KD) the mttp mRNA in the rat basophilic leukemia (RBL) cell line transfected with CD1d. Different siRNA were designed to generate several stable clones expressing various levels of residual mRNA (Fig. 3 A). Clones 3-9 and 20-1 expressing <20% residual MTP lost the ability to stimulate 2/2 Vα14 NKT hybridomas, DN32.D3 and N383c (Fig. 3 B). Clone 4-6, with only an ~50% reduction of MTP mRNA, exhibited only a modest defect in stimulation. Notably, stimulation of 3/3 non-Vα14 NKT hybridomas, TCB11, TBA7, and 1C8DC1, remained intact (Fig. 3 B). These selective defects match the well-established dichotomy between Vα14 and non-Vα14 NKT hybridomas with respect to their recognition of endosomal and nonendosomal ligands, respectively. They do not seem consistent, however, with the model suggesting that MTP critically regulates CD1d biosynthesis in the ER. Surface staining of CD1d in MTP-deficient clones showed a twofold reduction compared with the parental clone. Although these differences may be accounted for by interclonal variations, it is notable that the intracellular levels of CD1d were conserved or increased compared with WT. Thus, the intracellular/surface ratio was increased three- to fourfold in MTP KD cells, suggesting intracellular retention (Fig. 3 C). Notably, sorted RBL-CD1d cells expressing low levels of surface CD1d stimulated DN32.D3 well above the MTP-deficient RBL-CD1d clones, which express an intermediate level of surface CD1d, whereas stimulation of TBA7 was not altered by the absence of MTP (Fig. 3 D). Thus, a lower level of surface CD1d expression is unlikely to explain the selective Vα14 NKT cell stimulation defect.
Additional antigen presentation experiments were performed using a battery of well-defined synthetic GSL antigens (Fig. 4). The MTP KD clone 3-9 exhibited a partial defect in the presentation of αGC, which is known to depend on lysosomal loading, at least partially, for recognition by Vα14 NKT cells. The two disaccharide GSLs, α 1,2 α GalCer (PBS18) and α 1,4 α GalCer (PBS19), require lysosomal removal of the outer galactose before recognition by NKT cells. These two compounds were very poorly presented by the MTP KD clones, even at the highest concentrations. In addition, we found a profound defect in the presentation of exogenously added iGb3 or its precursor iGb4, both of which are also highly dependent on lysosomal trafficking of CD1d.
Because it is a direct prediction of the “ER chaperone” model that CD1d biosynthesis should be defective in the absence of MTP, we measured the rate of CD1d biosynthesis by immunoprecipitating CD1d in [S35]cystein and methionine metabolic pulse-chase experiments. As shown in Fig. 5 A, there was no significant decrease in the rate of biosynthesis or the rate of acquisition of Endo-H resistance, indicating intact ER synthesis and Golgi maturation of CD1d in the absence of MTP.
Altogether, our data suggested preservation of the secretory pathway and pointed to the lysosomal compartment as the site of impact of MTP deficiency. Using surface and intracellular flow cytometry staining for CD1d in MTP mutant and wild-type cells, we observed a conspicuous and consistent three- to fourfold increase of the intracellular over surface ratio of CD1d in MTP-deficient clones (Fig. 3 C). CD1d accesses the plasma membrane at two different stages of its life cycle: early, after biosynthesis by export from the ER via the Golgi to the cell surface, and late, after internalization from the plasma membrane to endosomal compartments from which CD1d recycles back and forth to the plasma membrane. Thus, the association of conserved synthesis and accumulation in late endosomal/lysosomal compartments (see below) suggested alterations in the late internalization/recycling events between plasma membrane and lysosome. Direct measurements of internalization rates using a surface biotinylation assay with cleavable biotin (15) unambiguously established that the rate of internalization was preserved in MTP-deficient cells (Fig. 5 B, left). Another assay based on surface staining with biotinylated anti-CD1d antibodies yielded identical results (Fig. 5 B, right). Altogether, the data pointed to a defect in the recycling of CD1d from internal late endosomal/lysosomal stores to the plasma membrane. We thus extended the surface biotinylation assay to follow the surface reexpression of a cohort of CD1d molecules that were previously internalized over a prolonged 7-h period to ensure trafficking to the lysosome. Cleavage of surface biotin was followed by a 4-h period of incubation, and a second cleavage removed the biotin associated with surface reexpression of CD1d. In the WT clone, the amount of intracellular biotinylated CD1d was reduced by >50% between strips I and II, demonstrating active recycling to the surface. In sharp contrast, the MTP KD clone 3-9 did not show recycling from intracellular compartments to the surface, as demonstrated by the lack of reduction of intracellular CD1d-biotin between strips I and II (Fig. 5 C). In fact, even the ~30% reduction normally seen after strip I could not be observed, likely reflecting the progressive lysosomal accumulation over the initial 7-h period. Similar results were found with another MTP KD clone, 20-1 (unpublished data). Impaired CD1d recycling from lysosomes to the cell surface is sufficient to explain the selective presentation defect for lipid antigens that require CD1d trafficking to the lysosomes as well as the decreased surface expression of CD1d.
Lysosomal dysfunction has not been previously observed in MTP-deficient cells. Confocal analysis showed normal gross morphology of the LAMP1+ late endosomal/lysosomal compartment of MTP-deficient cells and normal localization of the majority of intracellular CD1d in this compartment (Fig. 6 A, left). However, because some LAMP-1+ vesicles appeared to be larger in MTP-deficient than in WT cells, we performed a FACS quantification of the Lysotracker+ compartment and detected a 1.7- to twofold increase in the staining intensity of MTP-deficient cells in two separate experiments (Fig. 6 A, right). In a previous report, chemical inhibition of MTP did not impair presentation of ovalbumin to MHC class II–restricted peptide–specific OTII CD4 T cells. We directly confirmed that lysosomal degradation of hen egg lyzozyme (HEL) and horse radish peroxidase (HRP) proceeded with normal kinetics in the absence of MTP (Fig. 6 B). In addition, we studied the trafficking of exogenously administered PBS10, a fluorescent version of αGC tagged with prodan on C6" (16), and demonstrated normal trafficking to the late endosome and the lysosome (Fig. 6 C, left). However, another fluorescently labeled lipid, BODIPY-LacCer, which is known to accumulate in the Golgi after vesicular transport from the plasma membrane to the late endosome to the ER, showed abnormal retention in the Lysotracker+ compartment (Fig. 6 C, top right), suggesting the existence of at least some lipid trafficking defects in MTP-deficient cells. NBD-ceramide (Fig. 6 C, bottom right), which is rapidly transported from the plasma membrane to the ER by a nonendocytic pathway and then transferred to the Golgi by the cytosolic transporter CERT (ceramide transfer protein; reference 17), reached its destination normally in the absence of MTP. In an attempt to detect additional defects in lysosome-to-surface trafficking, we studied serotonin secretion, which occurs by granule exocytosis after exposure of RBL mast cells to IgE–DNP immune complexes (18). Fig. 6 D shows that Fc receptor–triggered serotonin secretion was conserved in MTP-deficient RBL cells. Altogether, these results suggest the existence of partial and selective lysosomal defects associated with MTP deficiency, including an increase in the overall size of the lysosomal compartment and an abnormal lysosomal retention of the lipid BODIPY-LacCer.
Although several proteins involved in the uptake, exchange, or transmembrane transport of lipids have been clearly involved in various aspects of CD1-mediated antigen presentation (3–8), the recently reported role of MTP remains somewhat elusive. The ER location of MTP, its coprecipitation with CD1d, and its well-established function in loading apoB with lipids have suggested that MTP might be a chaperone assisting the folding of nascent CD1d proteins through lipid loading. Indeed, chaperone-assisted folding and peptide loading are essential for MHC molecules, and it is logical to postulate that similar mechanisms might be associated with CD1 biosynthesis. However, some of the reported functional consequences of the genetic ablation or chemical inhibition of MTP were not fully consistent with this proposed function. For example, the decrease in CD1d surface expression was often modest and could not alone explain the profound defects in lipid antigen presentation seen upon genetic ablation of mttp (14). Likewise, chemical inhibition of MTP in two different human cell lines led to a defect in NKT cell stimulation without notable decrease in surface CD1d levels (13). In addition, when localization of CD1d was studied by confocal microscopy of liver sections, a majority of CD1d molecules were outside the ER, whether or not MTP was inactivated (14). Finally, although purified MTP could transfer phosphatidylethanolamine onto plastic wells coated with CD1d, the lipid transfer assay was indirect, and the stoichiometry of CD1d loading was not determined (13). Whether, like the well-studied saposins (3, 4), MTP can efficiently transfer lipids onto CD1d remains unclear.
We present direct cell biological and functional evidence suggesting an alternative explanation for the CD1d-mediated lipid presentation defects of MTP-deficient cells. Metabolic pulse-chase radiolabeling experiments established that the rates of CD1d biosynthesis and transit through ER and Golgi were unaltered in the absence of MTP. In addition, using a panel of well-characterized NKT hybridomas reactive to endogenous lipids, we found that, in the absence of MTP, CD1d-expressing cells stimulated the hybridomas responding to endogenous lipids loaded in the secretory compartment, whereas, in sharp contrast, they failed to stimulate those recognizing lysosomal antigens such as iGb3. Consistent with these in vitro findings, NKT cell development and stimulation by thymocytes were partially impaired in vivo in chimeric mice carrying a deletion of the first exon of MTP in their cortical thymocytes.
These surprising findings led us to consider the lysosomal stage of CD1d trafficking as a potential target of MTP. Previous studies have shown that, at steady state, most of the CD1d molecules normally reside in the lysosomal compartment, from which they undergo prolonged recycling back and forth to the plasma membrane. This movement is governed by a tyrosine motif in the intracytoplasmic tail of CD1d that binds AP-2 and AP-3 (19–21). CD1d tail-truncated mutants fail to present many lipids, including the Vα14 NKT ligand iGb3, or the di- or triglycosylated derivatives of αGalCer that require processing by lysosomal glycosidases before recognition by Vα14 NKT cells. Thus, the functional defects associated with the tail truncation of CD1d resemble those associated with MTP deficiency.
The intracellular distribution patterns, however, are opposite. Whereas tail-truncated CD1d exhibits a higher level of surface expression and decreased intracellular accumulation, the intracellular/surface ratio is increased in MTP-deficient cells. Importantly, the intracellular site of accumulation is the late endosome/lysosome rather than the ER. Thus, the conserved internalization rate and markedly decreased recycling identify a block in the exit pathway that normally allows CD1d molecules to return to the plasma membrane after they have loaded lipid antigens in the lysosome. This anomaly provides a potential explanation for all the cell biological and functional changes of CD1d trafficking and antigen presentation observed in MTP-deficient cells or animals. Because little is known about the molecular mechanisms controlling recycling in normal cells, there is at present no clear suggestion as to the precise molecular target of MTP. Reports that MHC class II presentation is conserved in MTP-deficient cells and our own findings that lysosomal degradation of HEL and HRP proteins are unaltered, that PBS10, a fluorescently labeled αGC, traffics normally to late endosome and lysosome, and that granule exocytosis is conserved in the absence of MTP suggest that the lysosomal defects are relatively selective. However, the size of the Lysotracker+ compartment appeared to be significantly increased, and a lysosomal retention of BODIPY-LacCer, which normally reaches the Golgi after endocytosis to the late endosome, could be detected as well. Thus, changes in the lipid composition of some membranes or lipid storage may alter the dynamics of the CD1d recycling pathway. Alternatively, MTP deficiency may impact another unidentified protein involved in recycling. Notably, recent experiments investigating CD1d-mediated lipid presentation defects associated with herpes simplex virus-1 infection have uncovered a very similar dysregulation of CD1d recycling from lysosome to plasma membrane (22), revealing the importance of this previously overlooked stage of CD1d trafficking and suggesting its relevance in host defense.
Our results do not rule out the possibility that, in the absence of MTP, aberrant lipid loading in the ER might prevent subsequent exchange with other lipid ligands in the lysosome and somehow alter CD1d recycling. This hypothesis, however, remains to be tested. Furthermore, although we found that 3/3 non-Vα14 autoreactive hybridomas (previously shown to respond to endogenous ligand loaded independently of lysosomal trafficking of CD1d) recognized CD1d in the absence of MTP, the response of another non-Vα14 hybridoma, 14S6, was reported to be altered (13), suggesting that, in addition to the major lysosomal defects, lipid presentation in the secretory pathway might be partially impaired as well.
In conclusion, MTP deficiency induces severe defects of lipid antigen presentation by CD1d. Despite the ER location of MTP, convergent cell biological and functional experiments indicate that a major impact on CD1d-mediated antigen presentation is in the altered recycling of CD1d from the lysosome to the plasma membrane. This surprising finding reveals a previously ignored, long-range effect of MTP in an important, yet poorly understood, intracellular trafficking process.
Mice with a “floxed” Mttp allele on a mixed C57BL/6 and 129S4/SvJae background (B6;129S-Mttptm2Sgy/J) were from The Jackson laboratory. These mice were bred with mice transgenic for Mx1 or Lck promoter-driven Cre recombinase on a C57BL/6 background (The Jackson Laboratory). Littermates were typed to determine Cre and floxed mttp presence by PCR according to a published protocol (23). The primer sequences used for mttp typing were 5′-GCTCTCAAGAGGAGGTTAAGG-3′ and 5′-CGTCTTTCAAGAGAATGCCC-3′. Detection of Mx1 or Lck Cre by PCR was done as described (The Jackson Laboratory). MTP-deficient mice and their WT littermates (nonfloxed mttp mice with or without Cre) were used for comparative analysis. CD1 KO mice in the C57BL/6 background were generated and maintained as described previously (24). All mice were raised in a specific pathogen-free environment at the University of Chicago, according to the Institutional Animal Care and Use Committee guidelines.
C57BL/6 CD1 KO mice received whole-body γ-irradiation (1,000 rad) with a cesium source (Gammacell 40; MDS Nordion) and were reconstituted 6 h later with one i.v. injection of 10 × 106 bone marrow cells from various adult donors as described. Mttpfl/fl Mx1-Cre mice and WT littermates were treated intraperitoneally with 400 μg dsRNA (polyI:C; Sigma-Aldrich) in PBS every day for 6 d, before bone marrow was collected and injected into irradiated CD1 KO mice.
Fresh thymocytes and splenocytes were obtained as described previously (25). Stably CD1d transfected RBL cells (25) were transfected with the vector pSUPER to form stable MTP KD siRNA clones (26), using selected sequences from the Rat mttp gene. The sequences used for the siRNA constructs were 5′-CCTCTGGAACCACCAATGA-3′ for clone 4-6, 5′-TTGCAGCCACTCCAGATGA-3′ for clone 20-1, and 5′-CTTGGAGGCTCTACGGAGA-3′ for clone 3-9. Clones were selected with 12.5 μg/ml puromycine, and MTP mRNA levels were detected by RT-PCR (CLONTECH Laboratories, Inc.). The sense and antisense primers for Rat mttp were 5′-GTCACGATAACGGCTGTCAATGTC-3′ and 5′-CCTCTCAATTTTGCATGTATCCAG-3′, respectively. For rat gapdh, primers were 5′-CTCTGGAAAGCTGTGGCGTGATGG-3′ and 5′-CTGTAGCCATATTCATTGTCATACC-3′.
Experiments were done as described previously (15). In brief, to study the biosynthesis and trafficking rate of CD1d from ER to the Golgi, cells were pulsed with 0.5 mCi/ml [S35]methionine and cystine (GE Healthcare) and chased with excess cold methionine and cysteine at 37°C for the indicated times. Cells were harvested, and CD1d molecules were immunoprecipitated with 20H2 antibody. Samples were treated or untreated with Endo-H according to the manufacturer's instructions (New England Biolabs, Inc.), before running on SDS-PAGE gel and analysis by phosphorimager.
Assays were as described previously (15). In brief, for the biotin internalization assay, surface proteins were biotinylated with a cleavable biotin reagent, Sulfo-NHS-S-S-Biotin (Pierce Chemical Co.; 0.5 mg/ml in HBSS at 4°C for 15 min). Cells were then incubated at 37°C for the indicated times, before stripping surface biotin with a glutathione solution. Levels of internalized CD1d molecules were detected by ELISA using 20H2 antibody to capture CD1d molecules and HRP-conjugated streptavidin (R&D Systems) to detect biotin and quantified by reference to standard CD1d-biotin. Residual biotinylated CD1d was compared with total biotinylated CD1d at time 0 (before internalization). For the antibody internalization assay, cells were surface stained with biotinylated anti-CD1d antibody (clone 1B1; BD Biosciences), washed, and incubated at 37°C for the indicated times. Remaining surface CD1d molecules were detected by flow cytometry using PE-conjugated streptavidin. For the recycling assay, surface proteins were biotinylated at 4°C and cells were incubated for 7 h at 37°C. Residual surface biotin was stripped as described above and cells were incubated for 4 h to allow recycling of internalized CD1d-biotin molecules to the surface. Surface biotin was stripped again, and residual biotinylated CD1d molecules were measured by ELISA as described.
CD1d-αGalCer tetramers were generated and used as described previously (27). FITC-conjugated anti-CD4, anti-CD1d, and anti-CD24; allophycocyanin-conjugated anti-B220; cychrome-conjugated anti-CD8; and PE-conjugated anti-CD1d were obtained from BD Biosciences. For the detection of surface and intracellular CD1d levels, RBL cells were stained with saturating amounts of FITC-conjugated anti-CD1d before wash, fixation, and permeabilization (Cytofix/Cytoperm; BD Biosciences) and staining with PE-conjugated anti-CD1d. Lysosomal staining was performed by incubating cells with LysotrackerRed DND99 (Invitrogen) at 1 μg/ml for 15 min. Flow cytometry was performed with FACSCalibur (Becton Dickinson), and data were analyzed using Cell Quest pro software (Becton Dickinson).
1 mg/ml HEL and HRP (Sigma-Aldrich) were incubated with RBL-CD1d and the MTP KD clone 3-9 for 4 h, before the cells were washed and harvested at the indicated time points. Residual protein levels were measured by Western blot for HEL (rabbit anti-lysozyme; ab391 [Abcam]) or intracellular flow cytometry for HRP (mouse anti-HRP; ab8326 [Abcam]). Data are representative of two independent experiments.
NKT hybridomas, including the Vα14-Jα18 DN32.D3 (25) and N383C (28) and the non-Vα14-Jα18 TCB11, TBA7, and 1C8DC1 clones (25), were incubated at 5 × 104 cells per 96 flat microwell in the presence of 5 × 105 fresh thymocytes or 5 × 104 RBL cells for 24 h in a 0.1-ml final volume of 1:1 mixture of Click's medium and RPMI supplemented with 10% heat-inactivated FCS, glutamine, antibiotics, and 5 × 10−5 M 2-ME as described. IL-2 released in cultured supernatants was measured using CTLL-2 indicator cells as described previously (29).
Determination of [3H]serotonin release was as described previously (18). In brief, 2 × 105 RBL cells were incubated with 0.4 μg/ml anti-DNP IgE (Sigma-Aldrich) in the presence of 2 μCi/ml [3H]serotonin (PerkinElmer) overnight. Cells were washed in modified Tyrode's buffer (135 mM NaCl, 5 mM Kcl, 1 mM MgCl2, 1.8 mM CaCl2, 5.6 mM dextrose, 10 mM Hepes, and 0.1% BSA) and activated at 37°C with 500 μl modified Tyrode's buffer containing 50 ng/ml DNP-HSA (Sigma-Aldrich) for 30 min. The reaction was stopped by placing the plate on ice and adding 500 μl of ice-cold modified Tyrode's buffer. Supernatant and cells were collected separately to measure serotonin release and uptake by the cells, respectively.
To study lipid trafficking, cells were incubated with 0.5 μM BODIPY-LacCer (Invitrogen) or with 9 μM prodan-conjugated αGalCer (PBS10; reference 16) at 37°C for 1 h or overnight, respectively. Cells were stained with LysoTrackerRed DND99 (Invitrogen) at 0.25 μg/ml during the last 15 min and directly analyzed by confocal microscopy (without fixation) or fixed before staining with rabbit anti-LAMP1 antibodies (ab24170; Abcam) and Cy5-conjugated donkey anti-rabbit antibodies (Jackson ImmunoResearch Laboratories). To study ceramide trafficking, cells grown on coverslips were incubated with 5 μM NBD-Cer (Invitrogen) at 37°C for 5 min. Coverslips were washed in PBS, and samples were immediately analyzed by confocal microscopy. FITC-conjugated rat anti-mouse LAMP1 antibodies (BD Biosciences) and biotin-conjugated anti-CD1d antibodies (BD Biosciences) were used to stain LAMP1 and CD1d in RBL cells as described previously (3). Cells were examined by confocal microscopy using a confocal microscope (TCS SP2 AOBS; Leica) with a 63× NA 1.4 oil objective lens at room temperature.
The authors thank members of the Bendelac Laboratory for advice and support and Dr. Stefan Kraft for help with the serotonin release assay.
Y. Sagiv is supported by a fellowship from the Cancer Research Institute. This work was supported by National Institutes of Health grants to A. Bendelac, P.B. Savage, and L. Teyton (PO1 AI053725).
The authors have no conflicting financial interests.
Abbreviations used: GSL, glycosphingolipid; HEL, hen egg lyzozyme; HRP, horse radish peroxidase; KD, knock down; MTP, microsomal triglyceride transfer protein; RBL, rat basophilic leukemia; siRNA, small interfering RNA.