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Thorax. 2007 June; 62(6): 559.
PMCID: PMC2117228

Lymphangioleiomyomatosis—presence of receptor tyrosine kinases and the angiogenesis factor VEGF‐A as potential therapeutic targets

Lymphangioleiomyomatosis (LAM) is a rare systemic disorder in women occurring either sporadically (sporadic LAM) or in association with tuberous sclerosis (TS‐LAM). It is caused by proliferating smooth muscle‐like LAM cells, which lead to a progressive cystic destruction of the lungs and abdominal tumours (renal angiomyolipomas and/or axial lymph node lesions). LAM cells express receptors for oestrogen and progesterone and stain positive for HMB‐45, an antibody against the melanoma‐related antigen.1 LAM fulfils the criteria of a neoplastic disease with enhanced proliferation,2 metastasising processes,3 increased migratory activity and invasiveness of LAM cells.4 Currently, an effective treatment interfering with these processes does not exist. Growth factors such as platelet‐derived growth factor (PDGF) and epidermal growth factor (EGF) have been identified to enhance LAM and renal angiomyolipoma cell proliferation in vitro.2,5 Whether LAM cells express growth factor‐associated receptor tyrosine kinases and the angiogenesis factor vascular endothelial growth factor‐A (VEGF‐A), which represent promising targets of small‐molecule and antibody therapy in neoplastic diseases, is currently unknown.

We studied immunohistochemically the expression of the following proteins by LAM cells in 10 formalin‐fixed and paraffin‐embedded LAM specimens: epidermal growth factor receptor (EGFR; PharmDx Kit, Dako, Hamburg, Germany), platelet‐derived growth factor receptor α (PDGFR‐α; rabbit polyclonal, Dianova, Hamburg, Germany), human epidermal growth factor receptor‐2 (HER2; HercepTest, Dako), VEGF‐A (clone VG1 identifying the VEGF‐A isoforms VEGF121, VEGF165 and VEGF189, DCS, Hamburg, Germany) and c‐KIT (CD117; rabbit polyclonal, Dako). Staining procedures were carried out according to the manufacturer's instructions, and appropriate positive and negative controls were used. A semiquantitative scoring system of the immunohistochemical reactions for all receptor tyrosine kinases, the hormone receptors and VEGF‐A was applied as follows: negative, no reaction or percentage of positive cells <5%; 1, 5–25% positive cells; 2, 26–50% positive cells; 3, 51–75% positive cells; 4, >75% positive cells; +, weak staining intensity; ++, moderate staining intensity; +++, strong staining intensity. Histological severity of lung destruction was assessed using the LAM histological score.6 The assessment of the LAM histological score and the immunohistochemical stainings was performed independently by two histopathologists (KE and MA). Only morphologically clear‐cut, HMB‐45 positive LAM lesions (nodules, cysts and diffuse LAM cell proliferations) were taken for analysis. All final decisions were made by consensus. Additionally, EGFR gene copy number per LAM cell nucleus was investigated by one histopathologist (SL) using fluorescence in situ hybridisation (FISH; LSI EGFR SpectrumOrange/CEP 7 SpectrumGreen probe, Vysis, Abbott Laboratories, Wiesbaden, Germany). The study was approved by the local ethics committee and written informed consent was obtained from all participants or their close relatives.

In all specimens, LAM lesions were consistently positive for PDGFR‐α and VEGF‐A. EGFR‐positive LAM cells were observed in seven specimens. No amplification or higher polysomy of the EGFR gene was detected. In addition to c‐KIT‐positive mast cells, which were sporadically present in LAM lesions and the surrounding lung tissue, LAM cells themselves were found to be positive for c‐KIT in six of the specimens. HER2 was negative in all specimens (fig 11).). For details, see supplementary table available online at http://www.thorax.bmjjournals.com/supplemental.

figure tx71811.f1
Figure 1 Expression of epidermal growth factor receptor (EGFR), platelet‐derived growth factor receptor α (PDGFR‐α), vascular endothelial growth factor‐A (VEGF‐A) and c‐KIT (CD117) in lung ...

We demonstrated that PDGFR‐α, EGFR, c‐KIT and VEGF‐A as targets of currently available compounds are expressed by LAM cells. These findings imply further research in the field of small‐molecule and antibody therapy in LAM.

Acknowledgements

We thank Professor H Morr, Dr J Linke, Dr J Galle, Dr C Smaczny and Dr M Choschzick for providing us with the tissue samples. We also thank Ralf Lieberz and his team for the excellent technical assistance.

A supplementary table is available online at http://www.thorax.bmj.com/supplemental

Supplementary Material

[web only table]

Footnotes

Competing interests: None.

A supplementary table is available online at http://www.thorax.bmj.com/supplemental

References

1. Johnson S R. Lymphangioleiomyomatosis. Eur Respir J 2006. 271056–1065.1065 [PubMed]
2. Goncharova E A, Goncharov D A, Spaits M. et al Abnormal growth of smooth muscle‐like cells in lymphangioleiomyomatosis: role for tumor suppressor TSC2. Am J Respir Cell Mol Biol 2006. 34561–572.572 [PMC free article] [PubMed]
3. Crooks D M, Pacheco‐Rodriguez G, Decastro R M. et al Molecular and genetic analysis of disseminated neoplastic cells in lymphangioleiomyomatosis. Proc Natl Acad Sci USA 2004. 10117462–17467.17467 [PubMed]
4. Goncharova E A, Goncharov D A, Lim P N. et al Modulation of cell migration and invasiveness by tumor suppressor TSC2 in lymphangioleiomyomatosis. Am J Respir Cell Mol Biol 2006. 34473–480.480 [PMC free article] [PubMed]
5. Lesma E, Grande V, Carelli S. et al Isolation and growth of smooth muscle‐like cells derived from tuberous sclerosis complex‐2 human renal angiomyolipoma: epidermal growth factor is the required growth factor. Am J Pathol 2005. 1671093–1103.1103 [PubMed]
6. Matsui K, Beasley M B, Nelson W K. et al Prognostic significance of pulmonary lymphangioleiomyomatosis histologic score. Am J Surg Pathol 2001. 25479–484.484 [PubMed]

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