PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. 1988 December; 170(12): 5607–5612.
PMCID: PMC211658

Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis.

Abstract

Streptomyces avermitilis contains a unique restriction system that restricts plasmid DNA containing N6-methyladenine or 5-methylcytosine. Shuttle vectors isolated from Escherichia coli RR1 or plasmids isolated from modification-proficient Streptomyces spp. cannot be directly introduced into S. avermitilis. This restriction barrier can be overcome by first transferring plasmids into Streptomyces lividans or a modification-deficient E. coli strain and then into S. avermitilis. The transformation frequency was reduced greater than 1,000-fold when plasmid DNA was modified by dam or TaqI methylases to contain N6-methyladenine or by AluI, HhaI, HphI methylases to contain 5-methylcytosine. Methyl-specific restriction appears to be common in Streptomyces spp., since either N6-methyladenine-specific or 5-methylcytosine-specific restriction was observed in seven of nine strains tested.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bailey CR, Winstanley DJ. Inhibition of restriction in Streptomyces clavuligerus by heat treatment. J Gen Microbiol. 1986 Oct;132(10):2945–2947. [PubMed]
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Brooks JE, Blumenthal RM, Gingeras TR. The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. Nucleic Acids Res. 1983 Feb 11;11(3):837–851. [PMC free article] [PubMed]
  • Burg RW, Miller BM, Baker EE, Birnbaum J, Currie SA, Hartman R, Kong YL, Monaghan RL, Olson G, Putter I, et al. Avermectins, new family of potent anthelmintic agents: producing organism and fermentation. Antimicrob Agents Chemother. 1979 Mar;15(3):361–367. [PMC free article] [PubMed]
  • Butkus V, Klimasauskas S, Kersulyte D, Vaitkevicius D, Lebionka A, Janulaitis A. Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate. Nucleic Acids Res. 1985 Aug 26;13(16):5727–5746. [PMC free article] [PubMed]
  • Clewell DB, Helinski DR. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol. 1972 Jun;110(3):1135–1146. [PMC free article] [PubMed]
  • Cox KL, Baltz RH. Restriction of bacteriophage plaque formation in Streptomyces spp. J Bacteriol. 1984 Aug;159(2):499–504. [PMC free article] [PubMed]
  • Ehrlich M, Wilson GG, Kuo KC, Gehrke CW. N4-methylcytosine as a minor base in bacterial DNA. J Bacteriol. 1987 Mar;169(3):939–943. [PMC free article] [PubMed]
  • Foor F, Roberts GP, Morin N, Snyder L, Hwang M, Gibbons PH, Paradiso MJ, Stotish RL, Ruby CL, Wolanski B, et al. Isolation and characterization of the Streptomyces cattleya temperate phage TG1. Gene. 1985;39(1):11–16. [PubMed]
  • Holmes DS, Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. [PubMed]
  • Hopwood DA, Kieser T, Wright HM, Bibb MJ. Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol. 1983 Jul;129(7):2257–2269. [PubMed]
  • Hopwood DA, Malpartida F, Kieser HM, Ikeda H, Duncan J, Fujii I, Rudd BA, Floss HG, Omura S. Production of 'hybrid' antibiotics by genetic engineering. Nature. 1985 Apr 18;314(6012):642–644. [PubMed]
  • Jones MD, Mabe JA, Nakatsukasa WM, Fayerman JT. pFJ265, a new cloning vehicle for Streptomyces. Plasmid. 1984 Jan;11(1):92–95. [PubMed]
  • Kessler C, Höltke HJ. Specificity of restriction endonucleases and methylases--a review. Gene. 1986;47(1):1–153. [PubMed]
  • Kieser T, Hopwood DA, Wright HM, Thompson CJ. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet. 1982;185(2):223–228. [PubMed]
  • Lacks S, Greenberg B. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol. 1977 Jul;114(1):153–168. [PubMed]
  • Larson JL, Hershberger CL. Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581. J Bacteriol. 1984 Jan;157(1):314–317. [PMC free article] [PubMed]
  • Larson JL, Hershberger CL. The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid. 1986 May;15(3):199–209. [PubMed]
  • MacNeil T, Gibbons PH. Characterization of the Streptomyces plasmid pVE1. Plasmid. 1986 Nov;16(3):182–194. [PubMed]
  • Matsushima P, Cox KL, Baltz RH. Highly transformable mutants of Streptomyces fradiae defective in several restriction systems. Mol Gen Genet. 1987 Mar;206(3):393–400. [PubMed]
  • Okanishi M, Suzuki K, Umezawa H. Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study. J Gen Microbiol. 1974 Feb;80(2):389–400. [PubMed]
  • Paradiso MJ, Roberts G, Streicher SL, Goldberg RB. Characterization of suppressible mutations in the viomycin phosphotransferase gene of the Streptomyces enteric plasmid pVE138. J Bacteriol. 1987 Mar;169(3):1325–1327. [PMC free article] [PubMed]
  • Raleigh EA, Wilson G. Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070–9074. [PubMed]
  • Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1985;13 (Suppl):r165–r200. [PMC free article] [PubMed]
  • Rodicio MR, Bruton CJ, Chater KF. New derivatives of the Streptomyces temperate phage phi C31 useful for the cloning and functional analysis of Streptomyces DNA. Gene. 1985;34(2-3):283–292. [PubMed]
  • Russell DW, Zinder ND. Hemimethylation prevents DNA replication in E. coli. Cell. 1987 Sep 25;50(7):1071–1079. [PubMed]
  • Sànchez J, Barbès C, Hernandez A, de los Reyes Gavilàn CR, Hardisson C. Restriction-modification systems in Streptomyces antibioticus. Can J Microbiol. 1985 Oct;31(10):942–946. [PubMed]
  • Sladek TL, Nowak JA, Maniloff J. Mycoplasma restriction: identification of a new type of restriction specificity for DNA containing 5-methylcytosine. J Bacteriol. 1986 Jan;165(1):219–225. [PMC free article] [PubMed]
  • Thompson CJ, Kieser T, Ward JM, Hopwood DA. Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction. Gene. 1982 Nov;20(1):51–62. [PubMed]
  • Thompson CJ, Ward JM, Hopwood DA. Cloning of antibiotic resistance and nutritional genes in streptomycetes. J Bacteriol. 1982 Aug;151(2):668–677. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)