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J Bacteriol. Aug 1988; 170(8): 3789–3791.
PMCID: PMC211366
Germination of Saccharomyces cerevisiae ascospores without trehalose mobilization as revealed by in vivo 13C nuclear magnetic resonance spectroscopy.
C Donnini, P P Puglisi, A Vecli, and N Marmiroli
Institute of Genetics, University of Parma, Italy.
Abstract
Saccharomyces cerevisiae ascospores germinate in the presence of acetate without any detectable trehalose degradation, as revealed by high-resolution nuclear magnetic resonance spectroscopy and by a standard colorimetric assay. The results presented here substantiate the hypothesis that in S. cerevisiae trehalose supplies energy during dormancy of the spores and not during the germination process.
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