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J Cell Biol. 1982 June 1; 93(3): 938–949.
PMCID: PMC2112136

Centrioles in the cell cycle. I. Epithelial cells


A study was made of the structure of the centrosome in the cell cycle in a nonsynchronous culture of pig kidney embryo (PE) cells. In the spindle pole of the metaphase cell there are two mutually perpendicular centrioles (mother and daughter) which differ in their ultrastructure. An electron-dense halo, which surrounds only the mother centriole and is the site where spindle microtubules converge, disappears at the end of telophase. In metaphase and anaphase, the mother centriole is situated perpendicular to the spindle axis. At the beginning of the G1 period, pericentriolar satellites are formed on the mother centriole with microtubules attached to them; the two centrioles diverge. The structures of the two centrioles differ throughout interphase; the mother centriole has appendages, the daughter does not. Replication of the centrioles occurs approximately in the middle of the S period. The structure of the procentrioles differs sharply from that of the mature centriole. Elongation of procentrioles is completed in prometaphase, and their structure undergoes a number of successive changes. In the G2 period, pericentriolar satellites disappear and some time later a fibrillar halo is formed on both mother centrioles, i.e., spindle poles begin to form. In the cells that have left the mitotic cycle (G0 period), replication of centrioles does not take place; in many cells, a cilium is formed on the mother centriole. In a small number of cells a cilium is formed in the S and G2 periods, but unlike the cilium in the G0 period it does not reach the surface of the cell. In all cases, it locates on the centriole with appendages. At the beginning of the G1 period, during the G2 period, and in nonciliated cells in the G0 period, one of the centrioles is situated perpendicular to the substrate. On the whole, it takes a mature centriole a cycle and a half to form in PE cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Albrecht-Buehler G, Bushnell A. The orientation of centrioles in migrating 3T3 cells. Exp Cell Res. 1979 Apr;120(1):111–118. [PubMed]
  • Albrecht-Buehler G, Bushnell A. The ultrastructure of primary cilia in quiescent 3T3 cells. Exp Cell Res. 1980 Apr;126(2):427–437. [PubMed]
  • Anderson RG, Brenner RM. The formation of basal bodies (centrioles) in the Rhesus monkey oviduct. J Cell Biol. 1971 Jul;50(1):10–34. [PMC free article] [PubMed]
  • Andersson LC, Lehto VP, Stenman S, Badley RA, Virtanen I. Diazepam induces mitotic arrest at prometaphase by inhibiting centriolar separation. Nature. 1981 May 21;291(5812):247–248. [PubMed]
  • Brinkley BR, Stubblefield E, Hsu TC. The effects of colcemid inhibition and reversal on the fine structure of the mitotic apparatus of Chinese hamster cells in vitro. J Ultrastruct Res. 1967 Jul;19(1):1–18. [PubMed]
  • DeFoor PH, Stubblefield E. Effects of actinomycin D, amethopterin, and 5-fluro-2'-deoxyuridine on procentriole formation in Chinese hamster fibroblasts in culture. Exp Cell Res. 1974 Mar 30;85(1):136–142. [PubMed]
  • Dippell RV. The development of basal bodies in paramecium. Proc Natl Acad Sci U S A. 1968 Oct;61(2):461–468. [PubMed]
  • Erickson HP. Assembly of microtubules from preformed, ring-shaped protofilaments and 6-S tubulin. J Supramol Struct. 1974;2(2-4):393–411. [PubMed]
  • Erlandson RA, de Harven E. The ultrastructure of synchronized HeLa cells. J Cell Sci. 1971 Mar;8(2):353–397. [PubMed]
  • Kalnins VI, Porter KR. Centriole replication during ciliogenesis in the chick tracheal epithelium. Z Zellforsch Mikrosk Anat. 1969;100(1):1–30. [PubMed]
  • McGill M, Brinkley BR. Human chromosomes and centrioles as nucleating sites for the in vitro assembly of microtubules from bovine brain tubulin. J Cell Biol. 1975 Oct;67(1):189–199. [PMC free article] [PubMed]
  • Murray RG, Murray AS, Pizzo A. The fine structure of mitosis in rat thymic lymphocytes. J Cell Biol. 1965 Aug;26(2):601–619. [PMC free article] [PubMed]
  • Pepper DA, Brinkley BR. Localization of tubulin in the mitotic apparatus of mammalian cells by immunofluorescence and immunoelectron microscopy. Chromosoma. 1977 Apr 19;60(3):223–235. [PubMed]
  • Rattner JB, Berns MW. Distribution of microtubules during centriole separation in rat kangaroo (Potorous) cells. Cytobios. 1976;15(57):37–43. [PubMed]
  • Rattner JB, Phillips SG. Independence of centriole formation and DNA synthesis. J Cell Biol. 1973 May;57(2):359–372. [PMC free article] [PubMed]
  • Rieder CL, Jensen CG, Jensen LC. The resorption of primary cilia during mitosis in a vertebrate (PtK1) cell line. J Ultrastruct Res. 1979 Aug;68(2):173–185. [PubMed]
  • REYNOLDS ES. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. [PMC free article] [PubMed]
  • Robbins E, Jentzsch G, Micali A. The centriole cycle in synchronized HeLa cells. J Cell Biol. 1968 Feb;36(2):329–339. [PMC free article] [PubMed]
  • Rodionov VI, Gelbfand VI, Rozenblat VA. 32S-oligomer tubulina. Ustoichivost'k faktoram, podavliaiushchim obrazovanie mikrotrubochek. Biokhimiia. 1976 Nov;41(11):2068–2074. [PubMed]
  • Roos UP. Light and electron microscopy of rat kangaroo cells in mitosis. I. Formation and breakdown of the mitotic apparatus. Chromosoma. 1973;40(1):43–82. [PubMed]
  • Snyder JA, McIntosh JR. Initiation and growth of microtubules from mitotic centers in lysed mammalian cells. J Cell Biol. 1975 Dec;67(3):744–760. [PMC free article] [PubMed]
  • Sorokin SP. Reconstructions of centriole formation and ciliogenesis in mammalian lungs. J Cell Sci. 1968 Jun;3(2):207–230. [PubMed]
  • Stubblefield E, Brinkley BR. Cilia formation in Chinese hamster fibroblasts in vitro as a response to colcemid treatment. J Cell Biol. 1966 Sep;30(3):645–652. [PMC free article] [PubMed]
  • Tucker RW, Pardee AB, Fujiwara K. Centriole ciliation is related to quiescence and DNA synthesis in 3T3 cells. Cell. 1979 Jul;17(3):527–535. [PubMed]
  • Vorobjev IA, Chentsov YS. The ultrastructure of centriole in mammalian tissue culture cells. Cell Biol Int Rep. 1980 Nov;4(11):1037–1044. [PubMed]

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