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J Cell Biol. 1978 July 1; 78(1): 8–27.
PMCID: PMC2110168

Flagellar elongation and shortening in Chlamydomonas. IV. Effects of flagellar detachment, regeneration, and resorption on the induction of flagellar protein synthesis

Abstract

Synthesis of new proteins is required to regenerate full length Chlamydomonas flagella after deflagellation. Using gametes, which have a low basal level of protein synthesis, it has been possible to label and detect the synthesis of many flagellar proteins in whole cells. The deflagellation-induced synthesis of the tubulins, dyneins, the flagellar membrane protein, and at least 20 other proteins which co- migrate with proteins in isolated axonemes, can be detected in gamete cytoplasm, and the times of initiation and termination of synthesis for each of the proteins can be studied. The nature of the signal that stimulates the cell to initiate flagellar protein synthesis is unknown. Flagellar regeneration and accompanying pool depletion are not necessary for either the onset or termination of flagellar protein synthesis, because colchicine, which blocks flagellar regeneration, does not change the pattern of proteins synthesized in the cytoplasm after deflagellation or the timing of their synthesis. Moreover, flagellar protein synthesis is stimulated after cells are chemically induced to resorb their flagella, indicating that the act of deflagellation itself is not necessary to stimulate synthesis. Methods were defined for inducing the cells to resorb their flagella by removing Ca++ from the medium and raising the concentration of K+ or Na+. The resorption was reversible and the flagellar components that were resorbed could be re-utilized to assemble flagella in the absence of protein synthesis. This new technique is used in this report to study the control of synthesis and assembly of flagella.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Behnke O, Forer A. Evidence for four classes of microtubules in individual cells. J Cell Sci. 1967 Jun;2(2):169–192. [PubMed]
  • Bloodgood RA. Resorption of organelles containing microtubules. Cytobios. 1974 Mar-Apr;9(35):142–161. [PubMed]
  • Castillo CJ, Hsiao CL, Coon P, Black LW. Identification and perperties of bacteriophage T4 capsid-formation gene products. J Mol Biol. 1977 Mar 5;110(3):585–601. [PubMed]
  • Coyne B, Rosenbaum JL. Flagellar elongation and shortening in chlamydomonas. II. Re-utilization of flagellar proteins. J Cell Biol. 1970 Dec;47(3):777–781. [PMC free article] [PubMed]
  • Dingle AD, Fulton C. Development of the flagellar apparatus of Naegleria. J Cell Biol. 1966 Oct;31(1):43–54. [PMC free article] [PubMed]
  • Fulton C, Kowit JD. Programmed synthesis of flagellar tubulin during cell differentiation in Naegleria. Ann N Y Acad Sci. 1975 Jun 30;253:318–332. [PubMed]
  • Givan AL, Criddle RS. Ribulosediphosphate carboxylase from Chlamydomonas reinhardi: purification, properties and its mode of synthesis in the cell. Arch Biochem Biophys. 1972 Mar;149(1):153–163. [PubMed]
  • Huang B, Rifkin MR, Luck DJ. Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol. 1977 Jan;72(1):67–85. [PMC free article] [PubMed]
  • Inoué S, Sato H. Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement. J Gen Physiol. 1967 Jul;50(6 Suppl):259–292. [PMC free article] [PubMed]
  • KATES JR, JONES RF. THE CONTROL OF GAMETIC DIFFERENTIATION IN LIQUID CULTURES OF CHLAMYDOMONAS. J Cell Physiol. 1964 Apr;63:157–164. [PubMed]
  • Kuriyama R. In vitro polymerization of flagellar and ciliary outer fiber tubulin into microtubules. J Biochem. 1976 Jul;80(1):153–165. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • LEWIN RA. Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol. 1954 Dec;11(3):358–363. [PubMed]
  • Linck RW. Comparative isolation of cilia and flagella from the lamellibranch mollusc, Aequipecten irradians. J Cell Sci. 1973 Mar;12(2):345–367. [PubMed]
  • Renaud FL, Rowe AJ, Gibbons IR. Some properties of the protein forming the outer fibers of cilia. J Cell Biol. 1968 Jan;36(1):79–90. [PMC free article] [PubMed]
  • Rosenbaum JL, Child FM. Flagellar regeneration in protozoan flagellates. J Cell Biol. 1967 Jul;34(1):345–364. [PMC free article] [PubMed]
  • SAGER R, GRANICK S. Nutritional studies with Chlamydomonas reinhardi. Ann N Y Acad Sci. 1953 Oct 14;56(5):831–838. [PubMed]
  • Salmon ED. Pressure-induced depolymerization of spindle microtubules. I. Changes in birefringence and spindle length. J Cell Biol. 1975 Jun;65(3):603–614. [PMC free article] [PubMed]
  • Stephens RE. Thermal fractionation of outer fiber doublet microtubules into A- and B-subfiber components. A- and B-tubulin. J Mol Biol. 1970 Feb 14;47(3):353–363. [PubMed]
  • Weeks DP, Collis PS. Induction of microtubule protein synthesis in Chlamydomonas reinhardi during flagellar regeneration. Cell. 1976 Sep;9(1):15–27. [PubMed]
  • Weeks DP, Collis P, Gealt MA. Control of induction of tubulin synthesis in Chlamydomonas reinhardi. Nature. 1977 Aug 18;268(5621):667–668. [PubMed]

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