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J Cell Biol. 1972 April 1; 53(1): 66–72.
PMCID: PMC2108690

SEPARATION OF MITOCHONDRIAL MEMBRANES OF NEUROSPORA CRASSA

II. Submitochondrial Localization of the Isoleucine-Valine Biosynthetic Pathway

Abstract

Separation of Neurospora mitochondrial outer membranes from the inner membrane/matrix fraction was effected by digitonin treatment and discontinuous density gradient centrifugation. The solubilization of four isoleucine-valine biosynthetic enzymes was studied as a function of digitonin concentration and time of incubation in the detergent. The kinetics of the appearance of valine biosynthetic function in fractions outside of the inner membrane/matrix fraction, coupled with enzyme solubilization patterns similar to that for the matrix marker, mitochondrial malate dehydrogenase, indicate that the four isoleucine-valine pathway enzymes are localized in the mitochondrial matrix.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Schnaitman C, Greenawalt JW. Enzymatic properties of the inner and outer membranes of rat liver mitochondria. J Cell Biol. 1968 Jul;38(1):158–175. [PMC free article] [PubMed]
  • Marco R, Sebastián J, Sols A. Location of the enzymes of the oxalacetate metabolic cross-roads in rat liver mitochondria. Biochem Biophys Res Commun. 1969 Mar 10;34(5):725–730. [PubMed]
  • Brdiczka D, Pette D, Brunner G, Miller F. Kompartimentierte Verteilung von Enzymen in Rattenlebermitochondrien. Eur J Biochem. 1968 Jul;5(2):294–304. [PubMed]
  • Norum KR, Farstad M, Bremer J. The submitochondrial distribution of acid:CoA ligase (AMP) and palmityl-CoA:carnitine palmityltransferase in rat liver mitochondria. Biochem Biophys Res Commun. 1966 Sep 8;24(5):797–804. [PubMed]
  • McKay R, Druyan R, Getz GS, Rabinowitz M. Intramitochondrial localization of delta-aminolaevulate synthetase and ferrochelatase in rat liver. Biochem J. 1969 Sep;114(3):455–461. [PubMed]
  • Sottocasa GL, Kuylenstierna B, Ernster L, Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. [PMC free article] [PubMed]
  • Wilson JE. Brain hexokinase. A proposed relation between soluble-particulate distribution and activity in vivo. J Biol Chem. 1968 Jul 10;243(13):3640–3647. [PubMed]
  • WAGNER RP, BERGQUIST A. Synthesis of valine and isoleucine in the presence of a particulate cell fraction of Neurospora. Proc Natl Acad Sci U S A. 1963 Jun;49:892–897. [PubMed]
  • Kuwana H, Caroline DF, Harding RW, Wagner RP. An acetohydroxy acid synthetase from Neurospora crassa. Arch Biochem Biophys. 1968 Oct;128(1):184–193. [PubMed]
  • ARMSTRONG FB, WAGNER RP. Biosynthesis of valine and isoleucine. IV. alpha-Hydroxy-beta-keto acid reductoisomerase of Salmonella. J Biol Chem. 1961 Jul;236:2027–2032. [PubMed]
  • Altmiller DH, Wagner RP. Purification and properties of dihydroxy acid dehydratase from soluble and mitochondrial fractions of Neurospora crassa. Arch Biochem Biophys. 1970 May;138(1):160–170. [PubMed]
  • Ghosh D, Forrest HS. Enzymatic studies on the hydroxylation of kynurenine in Drosophila melanogaster. Genetics. 1967 Mar;55(3):423–431. [PubMed]
  • Coleman MS, Armstrong FB. Branched-chain amino-acid aminotransferase of Salmonella typhimurium. I. Crystallization and preliminary characterization. Biochim Biophys Acta. 1971 Jan 13;227(1):56–66. [PubMed]
  • KIRITANI K, NARISE S, BERGQUIST A, WAGNER RP. THE OVERALL IN VITRO SYNTHESIS OF VALINE FROM PYRUVATE BY NEUROSPORA HOMOGENATES. Biochim Biophys Acta. 1965 May 4;100:432–443. [PubMed]
  • Bergquist A, LaBrie DA, Wagner RP. Amino acid synthesis by the mitochondria of Neurospora crassa. I. Dependence on respiration of mitochondria. Arch Biochem Biophys. 1969 Nov;134(2):401–407. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Burgoyne L, Case ME, Giles NH. Purification and properties of the aromatic (arom) synthetic enzyme aggregate of Neurospora crassa. Biochim Biophys Acta. 1969 Nov 4;191(2):452–462. [PubMed]
  • Mayer G, Staudinger H. Zur Lipoidabhängigkeit der L-Kynurenin-3-Hydroxylase. Hoppe Seylers Z Physiol Chem. 1967 May;348(5):599–600. [PubMed]

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