PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jcellbiolHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
 
J Cell Biol. 1963 September 1; 18(3): 663–679.
PMCID: PMC2106313

THE DIFFERENTIATION OF WHITE ADIPOSE CELLS

An Electron Microscope Study

Abstract

Differentiating white adipose tissue from presumptive and developing fat pads of newborn and young rats was fixed in buffered osmium tetroxide, embedded in Vestopal W, and examined in an electron microscope. Pre-adipose cells were found to be fibroblasts characterized by their spindle shape, long tenuous cytoplasmic extensions, and profuse endoplasmic reticulum. The developmental stages traced from fibroblast to mature adipose cell show a gradual change in cell shape, an accumulation of cytoplasm and non-membrane-bounded lipid, a decrease in the endoplasmic reticulum, and a change in shape of mitochondria. Transitory glycogen appears at mid-differentiation. Numerous smooth-membraned vesicles occur in the cytoplasm throughout differentiation. Pinocytosis is constantly evident. Cells of the multilocular stage are shown to differ from brown fat cells, particularly with respect to cytoplasmic membrane systems and mitochondria. No transport of particulate lipid from the lumen of the capillary to, or within, the adipose cell was detected, nor could any cell organelle be demonstrated to be visibly related to lipid synthesis and/or deposition.

Full Text

The Full Text of this article is available as a PDF (2.2M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BALBONI GC. [Observations on the ultrastructure of adipose tissue (white fat and brown fat)]. Boll Soc Ital Biol Sper. 1961 Sep 30;37:903–906. [PubMed]
  • BARRNETT RJ, BALL EG. Metabolic and ultrastructural changes induced in adipose tissue by insulin. J Biophys Biochem Cytol. 1960 Sep;8:83–101. [PMC free article] [PubMed]
  • CHASE WH. Structure of basement membranes of fat cells. AMA Arch Pathol. 1959 May;67(5):550–555. [PubMed]
  • CHASE WH. Fine structure of rat adipose tissue. J Ultrastruct Res. 1959 Mar;2(3):283–287. [PubMed]
  • FISHER ER, PAAR J. Carrageenin granuloma in the guinea pig and rat. I. Effect of hydrocortisone, estradiol and mast cell depletion on its histological and histo chemical features. Arch Pathol. 1960 Nov;70:565–575. [PubMed]
  • LEVER JD. The fine structure of brown adipose tissue in the rat with observations on the cytological changes following starvation and adrenalectomy. Anat Rec. 1957 Jul;128(3):361–377. [PubMed]
  • MILLONIG G. A modified procedure for lead staining of thin sections. J Biophys Biochem Cytol. 1961 Dec;11:736–739. [PMC free article] [PubMed]
  • NAPOLITANO L, FAWCETT D. The fine structure of brown adipose tissue in the newborn mouse and rat. J Biophys Biochem Cytol. 1958 Nov 25;4(6):685–692. [PMC free article] [PubMed]
  • PALAY SL, KARLIN LJ. An electron microscopic study of the intestinal villus. II. The pathway of fat absorption. J Biophys Biochem Cytol. 1959 May 25;5(3):373–384. [PMC free article] [PubMed]
  • SCHWARZ W, MERKER HJ, KUTZSCHE A. [Electron microscopic studies on fibrillogenesis in fibroblast cultures]. Z Zellforsch Mikrosk Anat. 1962;56:107–124. [PubMed]
  • SIDMAN RL. Histogenesis of brown adipose tissue in vivo and in organ culture. Anat Rec. 1956 Mar;124(3):581–601. [PubMed]
  • SIPERSTEIN MD. Inter-relationships of glucose and lipid metabolism. Am J Med. 1959 May;26(5):685–702. [PubMed]
  • TEDESCHI CG. "The fat cell; origin and structure". Conn Med. 1960 Jan;24:33–40. [PubMed]
  • WASSERMANN F, McDONALD TF. Electron microscopic investigation on the surface membrane structures of the fat-cell and of their changes during depletion of the cell. Z Zellforsch Mikrosk Anat. 1960;52:778–800. [PubMed]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press