Search tips
Search criteria 


Logo of wtpaEurope PMCEurope PMC Funders GroupSubmit a Manuscript
Nature. Author manuscript; available in PMC 2007 November 30.
Published in final edited form as:
PMCID: PMC2099462

This article has been retractedRetraction in: Nature. 2015 March 24; 468(7320): 122    See also: PMC Retraction Policy

The large-conductance Ca2+-activated K+ channel is essential for innate immunity


Neutrophil leukocytes have a pivotal function in innate immunity. Dogma dictates that the lethal blow is delivered to microbes by reactive oxygen species (ROS) and halogens1,2, products of the NADPH oxidase, whose impairment causes immunodeficiency. However, recent evidence indicates that the microbes might be killed by proteases, activated by the oxidase through the generation of a hypertonic, K+-rich and alkaline environment in the phagocytic vacuole3. Here we show that K+ crosses the membrane through large-conductance Ca2+-activated K+ (BKCa) channels. Specific inhibitors of these channels, iberiotoxin and paxilline, blocked oxidase-induced 86Rb+ fluxes and alkalinization of the phagocytic vacuole, whereas NS1619, a BKCa channel opener, enhanced both. Characteristic outwardly rectifying K+ currents, reversibly inhibited by iberiotoxin, were demonstrated in neutrophils and eosinophils and the expression of the α-subunit of the BK channel was confirmed by western blotting. The channels were opened by the combination of membrane depolarization and elevated Ca2+ concentration, both consequences of oxidase activity. Remarkably, microbial killing and digestion were abolished when the BKCa channel was blocked, revealing an essential and unexpected function for this K+ channel in the microbicidal process.

The NADPH oxidase is required for normal immunity1,2; where defective it results in chronic granulomatous disease (CGD)4. The oxidase transfers electrons from NADPH to oxygen, forming superoxide anions (O2) in the phagocytic vacuole4. This process is electrogenic5. The charge generated by the passage of electrons across the membrane is compensated for6 in part through a K+ flux3, which seems essential for microbial killing by these cells3. Because of the importance and novelty of this process, we have now sought the identity of the K+ channel involved.

The NADPH oxidase pumps O2 into the vacuole, which, together with its dismutation product O22, becomes protonated, consuming H+ ions. The observed increase in vacuolar pH (ref. 7) depends on charge compensation with ions other than protons from the cytoplasm. We predicted that blockade of the channel through which K+ enters the vacuole would cause pH to decrease, whereas opening the channels excessively would cause pH to increase above normal. Using fluorescein conjugated to Staphylococcus aureus as a pH indicator we found that modulators of the BKCa channel produced the appropriate alterations (Fig. 1a). Iberiotoxin (for which the concentration for 50% inhibition (IC50) is 9.7 nM) and paxilline (IC50 17 nM), both highly selective and potent inhibitors8,9, prevented alkalinization (Fig. 1a, b), as did the oxidase inhibitor diphenylene iodonium (DPI)5, whereas other K+ channel blockers—4-aminopyridine (4-AP)10, apamin11, glibenclamide12 and anandamide13—did not (Fig. 1a, b). The selective opener NS1619 (ref. 14) elevated pH above normal (Fig. 1a, b), unlike the KATP channel opener levcromakalim14 (Fig. 1a).

Figure 1
BKCa channels influence the pH within the phagocytic vacuole and 86Rb+ efflux from neutrophils and eosinophils. a, Vacuolar pH at 150 s (means ± s.e.m.; three asterisks, P < 0.001 compared with control). b, Time course of pH changes. Inset, ...

86Rb+ is commonly used as a surrogate for K+ in flux studies. When the oxidase is activated by 12-O-tetradecanoylphorbol-13-acetate (TPA), the O2 and 86Rb+ are expelled into the extracellular medium. Figure 1c shows that the 86Rb+ flux increased fourfold after stimulation with TPA; an efflux approaching this was also induced by opening the BKCa channel with NS1619 and was even further enhanced by combining this opener with TPA. The K+ efflux that resulted from stimulation with TPA was completely abrogated by iberiotoxin or paxilline, confirming that the efflux of 86Rb+ occurred through BKCa channels. The requirement for an active oxidase was shown by the inhibition of 86Rb+ flux by DPI. The release of the isotope induced by NS1619 was also completely abolished by iberiotoxin. Once again, 4-AP was without effect. Similar results were obtained with eosinophils (Fig. 1d).

The expression of the BKCa channels was detected in cell membranes and in membrane obtained from cytoplasmic granules (Fig. 1e), but not in the cytoplasm of neutrophils, by western blotting with an antibody to the α-subunit of the channel. Eosinophils contained about one-half of the amount of protein present in neutrophils (compare lanes 1 and 2 in Fig. 1e). Reverse-transcriptase-mediated polymerase chain reaction (RT–PCR) on mRNA derived from HL-60 cells with primers corresponding to the sequence of the α-subunit of the BKCa channel produced the predicted 479-base-pair product in cells induced with dimethyl sulphoxide (DMSO) to differentiate along the granulocyte lineage, but not in undifferentiated cells. The sequence of this PCR product corresponded exactly to nucleotides 2,822–3,301 of the hSlo complete coding sequence (U11717)15.

In patch-clamp studies16 of neutrophils, we observed small outward currents averaging about 250 pA at +140 mV under resting conditions. Current density varied from cell to cell; this might reflect variable activation of the oxidase by contact with the glass of the coverslip or pipette. After the addition of TPA, a large outwardly rectifying current developed at potentials positive to −30 mV, taking several minutes to develop, and tending to increase slightly with time thereafter. Small, variable inward currents, and inward tails, were observed at hyperpolarized potentials of less than −100 mV, and these became more obvious as the pulse times were increased. All these currents were completely and reversibly inhibited by iberiotoxin (Fig. 2a, upper panels, and b).

Figure 2
BKCa currents in granulocytes. a, Representative recordings from two neutrophils showing activation of outward currents by TPA and subsequent reversible inhibition by iberiotoxin (IbTx; upper panels) but not by Zn2+ (lower panels) in the presence of TPA. ...

The accepted model for such electrophysiological studies is the eosinophil16,17, and similar 86Rb+ fluxes (Fig. 1d) and TPA-activated outward currents, which were reversibly inhibited by iberiotoxin (Fig. 2c, top panels, and d), were also seen in these cells. Because the currents measured in eosinophils were only about one-third of those observed in neutrophils, this might suggest a lower oxidase activity in these cells, despite reports to the contrary18. We therefore measured the rates of both oxygen consumption and superoxide generation by these two cell types and showed both to be two to three times as rapid in neutrophils as in eosinophils under our experimental conditions (Fig. 2g).

In addition we performed cell-attached single-channel recordings from TPA-stimulated neutrophils and observed channels activated by depolarization with a characteristically large single-channel conductance15 (Fig. 2e).

It has been suggested that the charge induced by electron translocation through the NADPH oxidase (Ie) is compensated for by proton efflux5,6,16. One of the foundations for this assumption is that both Zn2+ and Cd2+, known proton channel blockers16,19, were also thought to inhibit O2 production6,16. We examined the effect of Zn2+ and Cd2+ on 86Rb+ release, which we expected to be depressed through an inhibition of Ie. Perversely, we found that 86Rb+ release was greater in TPA-stimulated neutrophils and eosinophils in their presence (Fig. 2f) and that Zn2+, at concentrations three orders of magnitude greater than those causing almost complete blockage of proton channels20, was also without effect on the currents from neutrophils (Fig. 2a, lower panels) and eosinophils (Fig. 2c, middle panels, and d). We therefore re-examined the influence of Zn2+ and Cd2+ on oxidase activity. Whereas oxygen consumption, the true measure of NADPH oxidase activity, was unaffected, both Zn2+ and Cd2+ almost completely inhibited the reduction of ferricytochrome c by O2 (Fig. 2g) by accelerating the dismutation of O2− to H2O2. In a system in which xanthine–xanthine-oxidase generated O2, 3 mM concentrations of these elements induced the dismutation of O2 to H2O2 at a rate indistinguishable from that catalysed by 1 μg ml−1 superoxide dismutase (Fig. 2h).

BKCa channels are classically opened by the combination of membrane depolarization and elevated cytosolic Ca2+ concentration ([Ca2+]c; ref. 21). The same was found to hold true for this channel in neutrophils (Fig. 3a) and eosinophils (Fig. 3b). Neither depolarizing the membrane by elevating external [K+] to 136 mM nor elevating the [Ca2+]c with the ionophore A23187, which produced a large fluorescence signal from the intracellular Ca2+ indicator Fluo-4 acetoxymethyl ester (AM) (Fig. 3g), was sufficient to open the K+ channel fully (Fig. 3a). 86Rb+ release was slightly increased by the addition of A23187, which is a weak stimulus for NADPH oxidase and membrane depolarization22. The combination of membrane depolarization and A23187 caused as much 86Rb+ efflux from both neutrophils and eosinophils (Fig. 3a, b) as did stimulation with TPA, and in both cell types this was totally blocked by iberiotoxin. Chelation of [Ca2+]c with bis-(o-aminophenoxy)ethaneN,N,N′,N′ -tetra-acetic acid (BAPTA) AM and extracellular EGTA also completely blocked 86Rb+ release from TPA-stimulated cells (Fig. 3a) without inhibiting oxygen consumption (not shown), strongly suggesting that TPA must raise [Ca2+]c.

Figure 3
Ca2+ and membrane depolarization open BKCa channels. a, b, Effects of TPA (hatched) in the presence or absence of other agents on 86Rb+ release from neutrophils (a) or eosinophils (b). IbTx, iberiotoxin. c, d, Traces of cells loaded with fura-2 and hydroethidine ...

Although TPA stimulation is well known to depolarize the neutrophil plasma membrane23, it is generally thought not to elevate [Ca2+]c (ref. 22). We therefore measured changes in [Ca2+]c after exposure to TPA. Using Fluo-4 to study [Ca2+] in cell populations (Fig. 3g), and fura-2 and indo-1 in fluorescence imaging at the level of the single cell24 (Fig. 3c, e), we found that an increase in [Ca2+]c coincided with initiation of the oxidase activity, measured with hydroethidine25 (Fig. 3c). The increase in [Ca2+]c was inhibited by DPI (Fig. 3d), linking the signalling pathway to the activity of the oxidase.

Increases in intracellular [Ca2+] were not blocked by chelating external Ca2+ with EGTA but were inhibited by pretreatment with the microsomal Ca2+-ATPase inhibitor thapsigargin26 (Fig. 3g), which depletes intracellular Ca2+ stores, indicating an intracellular source for the Ca2+. This posed the question of how this Ca2+ is mobilized by oxidase activity. One possibility seemed to be through a decrease in pH, because activation of the NADPH oxidase acidifies the cytosol27. In cells labelled with Fluo-4 AM, elevated [Ca2+]c was prevented by pretreatment with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP), and acidification of the cytosol after an acid loading protocol with NH4Cl (ref. 28) resulted in a rapid increase in [Ca2+]c. The oxidase pumps 4 mol l−1 of electrons into the vacuole3, and charge separation across the membrane leaves relatively large concentrations of protons on the cytosolic side. This was detected by confocal imaging of pHi with the dual-wavelength ratiometric indicator 5′-(and 6′)-carboxy-seminapthorhodofluor-1 (carboxy-SNARF), which revealed that TPA caused an intracellular acidification clearly localized beneath the plasma membrane (Fig. 3e, f). In addition, confocal imaging under ultraviolet with Indo-1 as a ratiometric dual-emission Ca2+ indicator also showed that the increase in [Ca2+]c was also predominantly localized under the plasma membrane in many cells, although in others it was more generalized.

Specific and complete inhibitors of the BKCa channel made it possible to selectively examine the role of K+ flux through BKCa channels, and the consequent elevation of vacuolar pH, in the killing of microbes3. Paxilline and iberiotoxin, but not 4-AP, completely inhibited the killing of S. aureus and Serratia marcescens—which classically infect CGD patients4—and Candida albicans (Fig. 4a-c). This failure of killing occurred despite totally normal oxidase activity, phagocytosis and iodination (Fig. 4e-g) and a normal composition of granule enzymes. The disruptive effects of channel block on the enzymatic digestion of dead bacteria were also striking (Fig. 4d).

Figure 4
Inhibitors of BKCa channels abolish microbial killing and digestion. a–c, Killing of S. aureus (a; n = 5), S. marcescens (b; n = 2) and C. albicans (c; n = 2) was inhibited by iberiotoxin and paxilline, whereas survival in the presence of 4-AP ...

These data have significance beyond the inherent value of defining the precise molecular mechanisms involved in a physiological process of paramount importance to survival. The perception that neutrophils kill microbes through toxic oxygen radicals and their metabolites provided much of the biological basis for the theories relating the toxicity of oxygen radicals to the pathogenesis of a wide variety of human diseases, and the development of antioxidant drugs for their treatment29. These theories and treatments merit re-evaluation.


Concentrations of reagents

Unless otherwise stated, the concentrations of reagents were as follows: A23187, 10 μM; apamin, 200 nM; anandamide, 3 μM; 4-AP, 4 mM; Cd2+, 3 mM; DPI, 5 μM; EGTA, 3 mM; glibenclamide, 10 μM; iberiotoxin, 100 nM; levcromakalim, 10 μM; NS1619, 30 μM; paxilline, 300 nM; TPA, 1 μgml−1; Zn2+, 3 mM; superoxide dismutase, 1 μg ml−1.

Purification of cells

Granulocytes were purified from human blood. Eosinophils were isolated by negative selection with anti-CD16 immunomagnetic beads16.

Measurement of vacuolar pH and [Ca2+]c

Vacuolar pH was determined from the excitation spectrum of fluorescein-labelled, IgG-coated S. aureus7. Neutrophils, purified from human blood in Dulbecco's phosphate-buffered saline (PBS; 140 mM NaCl, 10 mM KCl, 10 mM Na2H2PO4, 5 mM glucose pH 7.3) were plated into black-walled clear-based 96-well plates at a density of 1.5 × 107 per well, and mixed with inhibitors and/or openers for 3 min at 37°C. Fluorescein-labelled, IgG-coated S. aureus cells (2.5 × 106) were then mixed with the neutrophils and the fluorescence (λex 494 nm, λem 518 nm) from each well was measured at intervals in a fluorimetric imaging plate reader (FLIPR; Molecular Devices). A comparison of fluorescence was made with a calibration curve generated with free bacteria in buffers of different pH values. Curves in Fig. 1b were fitted by nonlinear regression analysis using the equation for a sigmoid concentration–response curve (GraphPad Prism, GraphPad) with the Hill coefficient constrained to one.

For measurements of [Ca2+]c, cells were plated on black-walled clear-based 96-well plates at a density of 106 per well, incubated with Dulbecco's PBS containing Fluo-4 AM (4 μM; Molecular Probes) for 2 h and washed three times to remove extracellular Fluo-4 AM. Fluorescence (λex 494 nm, λem 518 nm) from each well was measured before and after the addition of DPI and/or TPA and/or A23187. In thapsigargin experiments, neutrophils were preincubated for 30 min in PBS containing 500 nM thapsigargin and 3 mM EGTA, after which Fluo-4 AM was added. Washing steps were performed in Ca2+-free Dulbecco's PBS supplemented with 3 mM EGTA. In other experiments, neutrophils were preincubated in Ca2+-free Dulbecco's PBS containing 3 mM EGTA for 10 min before the addition of TPA. The effect of intracellular pH changes on [Ca2+] was studied with NH4Cl as described28.

Measurement of 86Rb+ fluxes

86Rb+ efflux was measured as described previously3. Labelled cells were incubated with iberiotoxin, paxilline or 4-AP for 3 min before stimulation with TPA. The calcium ionophore A23187, with BAPTA, EGTA and the channel openers NS1619 or levcromakalim, were added with or without TPA unless otherwise indicated. For depolarization studies, neutrophils or eosinophils were incubated in HEPES buffer containing 136 mM KCl.


Patch-clamp studies were performed on freshly isolated neutrophils and eosinophils. Membrane currents were recorded with an Axopatch 200B amplifier (Axon Instruments). Data were acquired and analysed with a Digidata interface (1200A or 1322; Axon Instruments) and pClamp software (version 6.0 and 8.0; Axon Instruments). Cells were voltage-clamped at −30 mV and pulsed for 400 ms from −100 mV to +140 mV in 20-mV increments. Currents were measured at the end of the pulse at +140 mV. Statistical analysis was performed by using one-way analysis of variance with a Bonferroni or Student–Newman–Keuls post hoc test. For the perforated-patch experiments, a stock solution of 100 mg ml−1 amphotericin B in DMSO was prepared and diluted in the pipette solution to give a final concentration of 200 μg ml−1 amphotericin B. Stable access was obtained after 20 min, and cell capacitance was about 1–5 pF. Series resistance was not routinely corrected for. Agents were applied to the bath with a gravity-driven perfusion system. The pipette solution contained (in mM): 140 KCl, 10 NaCl, 2 MgCl2, 0.7 CaCl2, 1 EGTA and 10 HEPES (made to pH 7.3 with KOH). Cells were perfused with an extracellular buffer containing (in mM): 140 NaCl, 2.5 KCl, 0.5 MgCl2, 1.2 CaCl2, 101 HEPES and 5 glucose (made to pH 7.4 with NaOH).

Confocal imaging

Cells were loaded with either 5 mM Indo-1 or 5 mM carboxy-SNARF AM for 20 min, washed in Hanks balanced salt solution and plated at low density on 22-mm glass coverslips, which were mounted on the stage of a Zeiss ultraviolet–visible confocal microscope equipped with a META detection system. Indo-1 fluorescence was excited at 351 nm and an emission ratio was acquired on two channels at 410 and 490 nm, and SNARF emission was detected at 530 and 580 nm, each with a 25-nm bandwidth. Indo-1 tends to bleach, so laser power was kept as low as possible; images were therefore a little noisy. The dual-emission ratiometric measurements are important here to avoid artefacts due to cell movement after stimulation. Oxidase activation or effective inhibition was confirmed with hydroethidine (1 mM) to monitor radical formation, excited at 543 nm and measured at 580–650 nm. Drugs were added to the chamber as concentrated stocks to reach the desired final concentration.

In addition, images of cells loaded with fura-2 AM (5 mM for 20 min) were acquired at low power on a cooled charge-coupled device-based imaging system. Fluorescence was excited alternately at 340 and 380 nm and measured at more than 510 nm.

Subcellular fractionation of neutrophils, western blotting and PCR

Plasma and granules were prepared from neutrophil post-nuclear supernatants, and granule membranes removed as described3. Membranes were purified at the interface of discontinuous sucrose gradients of 15% on 30% sucrose (w/w) western blots, which were processed as described15. Protein run on gel was as follows: homogenates of eosinophils and neutrophils, 18 μg; granule and plasma membranes, 10 μg; cytosol, 20 μg. In control experiments, the antibody was incubated with the immunizing peptide (50 μgml−1) for 30 min before use. RT–PCR of BKCa mRNA extracted from HL-60 cells before and after 5 days of DMSO-induced differentiation was as described15. The marker used was ΦX-174 Hae.

Measurements of neutrophil function in vitro

Microbial killing, digestion, iodination, oxygen consumption and superoxide generation were studied as described3. For killing studies, 108 neutrophils in 1 ml Dulbecco's PBS were preincubated with iberiotoxin, paxilline, 4-AP or NS1619 for 3 min in a rapidly stirred oxygenated chamber at 37°C, then mixed with 108 IgG-opsonized microbes. Phagocytosis was measured as described7 using fluorescent rather than radiolabelled bacteria.

Superoxide dismutase activity

H2O2 production was measured in a xanthine–xanthine-oxidase O2−-generating system by the bleaching of phenol red30.


We thank P. Rich for helpful discussions, S. Ranasinghe for technical help with the FLIPR, and A. Scott for the illustrations. Financial support was provided by the Wellcome Trust and Medical Research Council.


Competing interests statement The authors declare that they have no competing financial interests.


1. Klebanoff SJ. Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. Semin. Hematol. 1975;12:117–142. [PubMed]
2. Babior BM. Phagocytes and oxidative stress. Am. J. Med. 2000;109:33–44. [PubMed]
3. Reeves EP, et al. Killing activity of neutrophils is mediated through activation of proteases by K+ flux. Nature. 2002;416:291–297. [PubMed]
4. Thrasher AJ, Keep NH, Wientjes F, Segal AW. Chronic granulomatous disease. Biochim. Biophys. Acta. 1994;1227:1–24. [PubMed]
5. Henderson LM, Chappell JB, Jones OT. The superoxide-generating NADPH oxidase of human neutrophils is electrogenic and associated with an H+ channel. Biochem. J. 1987;246:325–329. [PubMed]
6. Henderson LM, Chappell JB, Jones OT. Superoxide generation by the electrogenic NADPH oxidase of human neutrophils is limited by the movement of a compensating charge. Biochem. J. 1988;255:285–290. [PubMed]
7. Segal AW, Geisow M, Garcia R, Harper A, Miller R. The respiratory burst of phagocytic cells is associated with a rise in vacuolar pH. Nature. 1981;290:406–409. [PubMed]
8. Galvez A, et al. Purification and characterization of a unique, potent, peptidyl probe for the high conductance calcium-activated potassium channel from venom of the scorpion Buthus tamulus. J. Biol. Chem. 1990;265:11083–11090. [PubMed]
9. Sanchez M, McManus OB. Paxilline inhibition of the alpha-subunit of the high-conductance calcium-activated potassium channel. Neuropharmacology. 1996;35:963–968. [PubMed]
10. Clapp LH, Tinker A. Potassium channels in the vasculature. Curr. Opin. Nephrol. Hypertens. 1998;7:91–98. [PubMed]
11. Garcia ML, et al. Use of toxins to study potassium channels. J. Bioenerg. Biomembr. 1991;23:615–646. [PubMed]
12. Santos-Silva JE, Santos-Silva MC, Cunha FDQ, Assreuy J. The role of ATP-sensitive potassium channels in neutrophil migration and plasma exudation. J. Pharmac. Exp. Ther. 2002;300:946–951. [PubMed]
13. Maingret F, Patel AJ, Lazdunski M, Honoré E. The endocannabinoid anandamide is a direct and selective blocker of the background K+ channel TASK-1. EMBO J. 2001;20:47–54. [PubMed]
14. Lawson K. Potassium channel openers as potential therapeutic weapons in ion channel disease. Kidney Int. 2000;57:838–845. [PubMed]
15. Fernandez-Fernandez JM, Nobles M, Currid A, Vazquez E, Valverde MA. Maxi K+ channel mediates regulatory volume decrease response in a human bronchial epithelial cell line. Am. J. Physiol. Cell Physiol. 2002;283:C1705–C1714. [PubMed]
16. DeCoursey TE, Morgan D, Cherny VV. The voltage dependence of NADPH oxidase reveals why phagocytes need proton channels. Nature. 2003;422:531–534. [PubMed]
17. Schrenzel J, et al. Electron currents generated by the human phagocyte NADPH oxidase. Nature. 1998;392:734–737. [PubMed]
18. Petreccia DC, Nauseef WM, Clark RA. Respiratory burst of normal human eosinophils. J. Leukoc. Biol. 1987;41:283–288. [PubMed]
19. Henderson LM, Chappell JB, Jones OT. Internal pH changes associated with the activity of NADPH oxidase of human neutrophils. Further evidence for the presence of an H+ conducting channel. Biochem. J. 1988;251:563–567. [PubMed]
20. Cherny VV, Henderson LM, Xu W, Thomas LL, DeCoursey TE. Activation of NADPH oxidase-related proton and electron currents in human eosinophils by arachidonic acid. J. Physiol. (Lond.) 2001;535:783–794. [PubMed]
21. Kaczorowski GJ, et al. High-conductance calcium activated potassium channel; structure, pharmacology, and function. J. Bioenerg. Biomembr. 1996;28:255–267. [PubMed]
22. Mahomed GA, Anderson R. Activation of human neutrophils with chemotactic peptide, opsonised zymosan and the calcium ionophore A23187, but not with a phorbol ester, is accompanied by efflux and store-operated influx of calcium. Inflammation. 2000;24:559–569. [PubMed]
23. Jankowski A, Grinstein S. A noninvasive fluorimetric procedure for measurement of membrane potential. Quantification of the NADPH oxidase-induced depolarization in activated neutrophils. J. Biol. Chem. 1999;274:26098–26104. [PubMed]
24. Tsien RY. Fluorescent indicators of ion concentrations. Methods Cell Biol. 1989;30:127–156. [PubMed]
25. Vergun O, et al. Exploration of the role of reactive oxygen species in glutamate neurotoxicity in rat hippocampal neurones in culture. J. Physiol. (Lond.) 2001;531:147–163. [PubMed]
26. Demaurex N, Monod A, Lew DP, Krause KH. Characterization of receptor-mediated and store-regulated Ca2+ influx in human neutrophils. Biochem. J. 1994;297:595–601. [PubMed]
27. Grinstein S, Furuya W. Cytoplasmic pH regulation in phorbol ester-activated human neutrophils. Am. J. Physiol. 1986;251:C55–C65. [PubMed]
28. Willoughby D, Thomas R, Schwiening C. The effects of intracellular pH changes on resting cytosolic calcium in voltage-clamped snail neurones. J. Physiol. (Lond.) 2001;530:405–416. [PubMed]
29. Salvemini D, Riley DP, Cuzzocrea S. SOD mimetics are coming of age. Nature Rev Drug Discov. 2002;1:367–374. [PubMed]
30. Pick E, Keisari YA. simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J. Immunol. Methods. 1980;38:161–170. [PubMed]