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J Bacteriol. Jun 1991; 173(12): 3911–3913.
PMCID: PMC208026
Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence.
C Elkins, C E Thomas, H S Seifert, and P F Sparling
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Piliated Neisseria gonorrhoeae are known to be transformed less readily if transforming DNA competes with DNA containing the 10-bp sequence GCCGTCTGAA. It has been postulated that the 10-bp sequence is a recognition sequence which is required for efficient DNA uptake. We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid.
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