Search tips
Search criteria 


Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1991 April; 173(8): 2707–2709.
PMCID: PMC207841

Nomenclature relating to restriction of modified DNA in Escherichia coli.


At least three restriction systems that attack DNA containing naturally modified bases have been found in common Escherichia coli K-12 strains. These systems are McrA, McrBC, and Mrr. A brief summary of the genetic and phenotypic properties so far observed in laboratory strains is set forth, together with a proposed nomenclature for describing these properties.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (568K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Blumenthal RM, Gregory SA, Cooperider JS. Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli. J Bacteriol. 1985 Nov;164(2):501–509. [PMC free article] [PubMed]
  • Dharmalingam K, Goldberg EB. Phage-coded protein prevents restriction of unmodified progeny T4 DNA. Nature. 1976 Apr 1;260(5550):454–456. [PubMed]
  • Dharmalingam K, Revel HR, Goldberg EB. Physical mapping and cloning of bacteriophage T4 anti-restriction endonuclease gene. J Bacteriol. 1982 Feb;149(2):694–699. [PMC free article] [PubMed]
  • Dila D, Sutherland E, Moran L, Slatko B, Raleigh EA. Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12. J Bacteriol. 1990 Sep;172(9):4888–4900. [PMC free article] [PubMed]
  • Grant SG, Jessee J, Bloom FR, Hanahan D. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4645–4649. [PubMed]
  • Heitman J, Model P. Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3243–3250. [PMC free article] [PubMed]
  • Kohler SW, Provost GS, Kretz PL, Dycaico MJ, Sorge JA, Short JM. Development of a short-term, in vivo mutagenesis assay: the effects of methylation on the recovery of a lambda phage shuttle vector from transgenic mice. Nucleic Acids Res. 1990 May 25;18(10):3007–3013. [PMC free article] [PubMed]
  • Kretz PL, Reid CH, Greener A, Short JM. Effect of lambda packaging extract mcr restriction activity on DNA cloning. Nucleic Acids Res. 1989 Jul 11;17(13):5409–5409. [PMC free article] [PubMed]
  • Noyer-Weidner M, Diaz R, Reiners L. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB. Mol Gen Genet. 1986 Dec;205(3):469–475. [PubMed]
  • Piekarowicz A, Yuan R, Stein DC. Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12. J Bacteriol. 1991 Jan;173(1):150–155. [PMC free article] [PubMed]
  • Raleigh EA, Murray NE, Revel H, Blumenthal RM, Westaway D, Reith AD, Rigby PW, Elhai J, Hanahan D. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res. 1988 Feb 25;16(4):1563–1575. [PMC free article] [PubMed]
  • Raleigh EA, Trimarchi R, Revel H. Genetic and physical mapping of the mcrA (rglA) and mcrB (rglB) loci of Escherichia coli K-12. Genetics. 1989 Jun;122(2):279–296. [PubMed]
  • Raleigh EA, Wilson G. Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070–9074. [PubMed]
  • Ravi RS, Sozhamannan S, Dharmalingam K. Transposon mutagenesis and genetic mapping of the rglA and rglB loci of Escherichia coli. Mol Gen Genet. 1985;198(3):390–392. [PubMed]
  • Revel HR. Restriction of nonglucosylated T-even bacteriophage: properties of permissive mutants of Escherichia coli B and K12. Virology. 1967 Apr;31(4):688–701. [PubMed]
  • Ross TK, Achberger EC, Braymer HD. Identification of a second polypeptide required for McrB restriction of 5-methylcytosine-containing DNA in Escherichia coli K12. Mol Gen Genet. 1989 Apr;216(2-3):402–407. [PubMed]
  • Ross TK, Achberger EC, Braymer HD. Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus. J Bacteriol. 1989 Apr;171(4):1974–1981. [PMC free article] [PubMed]
  • Ross TK, Braymer HD. Localization of a genetic region involved in McrB restriction by Escherichia coli K-12. J Bacteriol. 1987 Apr;169(4):1757–1759. [PMC free article] [PubMed]
  • Sozhamannan S, Dharmalingam K. Molecular cloning and identification of protein products of rglB locus of Escherichia coli. Gene. 1988 Dec 25;74(1):51–52. [PubMed]
  • van de Putte P, Plasterk R, Kuijpers A. A Mu gin complementing function and an invertible DNA region in Escherichia coli K-12 are situated on the genetic element e14. J Bacteriol. 1984 May;158(2):517–522. [PMC free article] [PubMed]
  • Whittaker PA, Campbell AJ, Southern EM, Murray NE. Enhanced recovery and restriction mapping of DNA fragments cloned in a new lambda vector. Nucleic Acids Res. 1988 Jul 25;16(14B):6725–6736. [PMC free article] [PubMed]
  • Woodcock DM, Crowther PJ, Diver WP, Graham M, Bateman C, Baker DJ, Smith SS. RglB facilitated cloning of highly methylated eukaryotic DNA: the human L1 transposon, plant DNA, and DNA methylated in vitro with human DNA methyltransferase. Nucleic Acids Res. 1988 May 25;16(10):4465–4482. [PMC free article] [PubMed]
  • Woodcock DM, Crowther PJ, Doherty J, Jefferson S, DeCruz E, Noyer-Weidner M, Smith SS, Michael MZ, Graham MW. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 1989 May 11;17(9):3469–3478. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)