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J Bacteriol. Feb 1991; 173(3): 1353–1356.
PMCID: PMC207264
Efficient transformation of Bacillus thuringiensis requires nonmethylated plasmid DNA.
A Macaluso and A M Mettus
Ecogen Inc., Langhorne, Pennsylvania 19047.
Abstract
The transformation efficiency of Bacillus thuringiensis depends upon the source of plasmid DNA. DNA isolated from B. thuringiensis, Bacillus megaterium, or a Dam- Dcm- Escherichia coli strain efficiently transformed several B. thuringiensis strains, B. thuringiensis strains were grouped according to which B. thuringiensis backgrounds were suitable sources of DNA for transformation of other B. thuringiensis strains, suggesting that B. thuringiensis strains differ in DNA modification and restriction. Efficient transformation allowed the demonstration of developmental regulation of cloned crystal protein genes in B. thuringiensis.
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