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J Bacteriol. 1991 January; 173(2): 933–936.
PMCID: PMC207095

Visualization of bacterial flagella by video-enhanced light microscopy.

Abstract

We have imaged individual flagellar filaments of Escherichia coli, a motile Streptococcus sp., and Rhizobium meliloti by video-enhanced differential interference-contrast microscopy (Nomarski DIC) and computer-based image processing. This approach has advantages over existing methods in that filaments on living cells can be seen over their entire lengths.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allen RD, Allen NS, Travis JL. Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: a new method capable of analyzing microtubule-related motility in the reticulopodial network of Allogromia laticollaris. Cell Motil. 1981;1(3):291–302. [PubMed]
  • Armstrong JB, Adler J, Dahl MM. Nonchemotactic mutants of Escherichia coli. J Bacteriol. 1967 Jan;93(1):390–398. [PMC free article] [PubMed]
  • Block SM, Blair DF, Berg HC. Compliance of bacterial flagella measured with optical tweezers. Nature. 1989 Apr 6;338(6215):514–518. [PubMed]
  • de Boer PA, Crossley RE, Rothfield LI. Isolation and properties of minB, a complex genetic locus involved in correct placement of the division site in Escherichia coli. J Bacteriol. 1988 May;170(5):2106–2112. [PMC free article] [PubMed]
  • Frazer AC, Curtiss R., 3rd Production, properties and utility of bacterial minicells. Curr Top Microbiol Immunol. 1975;69:1–84. [PubMed]
  • Götz R, Schmitt R. Rhizobium meliloti swims by unidirectional, intermittent rotation of right-handed flagellar helices. J Bacteriol. 1987 Jul;169(7):3146–3150. [PMC free article] [PubMed]
  • Johansen E, Finan TM, Gefter ML, Signer ER. Monoclonal antibodies to Rhizobium meliloti and surface mutants insensitive to them. J Bacteriol. 1984 Oct;160(1):454–457. [PMC free article] [PubMed]
  • Kudo S, Magariyama Y, Aizawa S. Abrupt changes in flagellar rotation observed by laser dark-field microscopy. Nature. 1990 Aug 16;346(6285):677–680. [PubMed]
  • Macnab RM. Examination of bacterial flagellation by dark-field microscopy. J Clin Microbiol. 1976 Sep;4(3):258–265. [PMC free article] [PubMed]
  • Macnab RM, DeRosier DJ. Bacterial flagellar structure and function. Can J Microbiol. 1988 Apr;34(4):442–451. [PubMed]
  • Macnab RM, Ornston MK. Normal-to-curly flagellar transitions and their role in bacterial tumbling. Stabilization of an alternative quaternary structure by mechanical force. J Mol Biol. 1977 May 5;112(1):1–30. [PubMed]
  • Maurer L, Orndorff PE. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili. J Bacteriol. 1987 Feb;169(2):640–645. [PMC free article] [PubMed]
  • Reeve JN, Mendelson NH, Coyne SI, Hallock LL, Cole RM. Minicells of Bacillus subtilis. J Bacteriol. 1973 May;114(2):860–873. [PMC free article] [PubMed]
  • Schnapp BJ. Viewing single microtubules by video light microscopy. Methods Enzymol. 1986;134:561–573. [PubMed]
  • Shimada K, Kamiya R, Asakura S. Left-handed to right-handed helix conversion in Salmonella flagella. Nature. 1975 Mar 27;254(5498):332–334. [PubMed]
  • Trachtenberg S, DeRosier DJ, Macnab RM. Three-dimensional structure of the complex flagellar filament of Rhizobium lupini and its relation to the structure of the plain filament. J Mol Biol. 1987 Jun 5;195(3):603–620. [PubMed]
  • van der Drift C, Duiverman J, Bexkens H, Krijnen A. Chemotaxis of a motile Streptococcus toward sugars and amino acids. J Bacteriol. 1975 Dec;124(3):1142–1147. [PMC free article] [PubMed]

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