Search tips
Search criteria 


Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1993 October; 175(19): 6354–6357.
PMCID: PMC206735

Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.


Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (691K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Behnke D. Plasmid transformation of Streptococcus sanguis (Challis) occurs by circular and linear molecules. Mol Gen Genet. 1981;182(3):490–497. [PubMed]
  • Behnke D, Malke H, Hartmann M, Walter F. Post-transformational rearrangement of an in vitro reconstructed group-A streptococcal erythromycin resistance plasmid. Plasmid. 1979 Oct;2(4):605–616. [PubMed]
  • Clewell DB, Yagi Y, Dunny GM, Schultz SK. Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J Bacteriol. 1974 Jan;117(1):283–289. [PMC free article] [PubMed]
  • Daneo-Moore L, Volpe A. Recombination-deficient Streptococcus sanguis. Infect Immun. 1985 May;48(2):584–586. [PMC free article] [PubMed]
  • Dybvig K, Hollingshead SK, Heath DG, Clewell DB, Sun F, Woodard A. Degenerate oligonucleotide primers for enzymatic amplification of recA sequences from gram-positive bacteria and mycoplasmas. J Bacteriol. 1992 Apr;174(8):2729–2732. [PMC free article] [PubMed]
  • Frandsen EV, Pedrazzoli V, Kilian M. Ecology of viridans streptococci in the oral cavity and pharynx. Oral Microbiol Immunol. 1991 Jun;6(3):129–133. [PubMed]
  • Lawson JW, Gooder H. Growth and development of competence in the group H streptococci. J Bacteriol. 1970 Jun;102(3):820–825. [PMC free article] [PubMed]
  • LeBlanc DJ, Lee LN, Abu-Al-Jaibat A. Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. Plasmid. 1992 Sep;28(2):130–145. [PubMed]
  • LeBlanc DJ, Hassell FP. Transformation of Streptococcus sanguis Challis by plasmid deoxyribonucleic acid from Streptococcus faecalis. J Bacteriol. 1976 Oct;128(1):347–355. [PMC free article] [PubMed]
  • Macrina FL, Tobian JA, Jones KR, Evans RP. Molecular cloning in the Streptococci. Basic Life Sci. 1982;19:195–210. [PubMed]
  • Macrina FL, Wood PH, Jones KR. Genetic transformation of Streptococcus sanguis (Challis) with cryptic plasmids from Streptococcus ferus. Infect Immun. 1980 Jun;28(3):692–699. [PMC free article] [PubMed]
  • Miller RV, Kokjohn TA. General microbiology of recA: environmental and evolutionary significance. Annu Rev Microbiol. 1990;44:365–394. [PubMed]
  • Parker MT, Ball LC. Streptococci and aerococci associated with systemic infection in man. J Med Microbiol. 1976 Aug;9(3):275–302. [PubMed]
  • Quivey RG, Jr, Faustoferri RC. In vivo inactivation of the Streptococcus mutans recA gene mediated by PCR amplification and cloning of a recA DNA fragment. Gene. 1992 Jul 1;116(1):35–42. [PubMed]
  • Raina JL, Macrina FL. A competence specific inducible protein promotes in vivo recombination in Streptococcus sanguis. Mol Gen Genet. 1982;185(1):21–29. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Sulavik MC, Tardif G, Clewell DB. Identification of a gene, rgg, which regulates expression of glucosyltransferase and influences the Spp phenotype of Streptococcus gordonii Challis. J Bacteriol. 1992 Jun;174(11):3577–3586. [PMC free article] [PubMed]
  • Terleckyj B, Willett NP, Shockman GD. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun. 1975 Apr;11(4):649–655. [PMC free article] [PubMed]
  • Yagi Y, Clewell DB. Recombination-deficient mutant of Streptococcus faecalis. J Bacteriol. 1980 Aug;143(2):966–970. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)