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J Bacteriol. Oct 1993; 175(19): 6354–6357.
PMCID: PMC206735
Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.
M M Vickerman, D G Heath, and D B Clewell
Department of Cariology, School of Dentistry, University of Michigan, Ann Arbor 48109.
Abstract
Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.
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