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J Bacteriol. 1992 March; 174(5): 1682–1685.
PMCID: PMC206566

Quantitation of Dam methyltransferase in Escherichia coli.


An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

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