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J Bacteriol. 1992 March; 174(5): 1682–1685.
PMCID: PMC206566

Quantitation of Dam methyltransferase in Escherichia coli.

Abstract

An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

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