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Cerebral cavernous malformation (CCM), a disease associated with defective endothelial junctions, result from autosomal dominant CCM1 mutations that cause loss of KRIT-1 protein function, though how the loss of KRIT-1 leads to CCM is obscure. KRIT-1 binds to Rap1, a guanosine triphosphatase that maintains the integrity of endothelial junctions. Here, we report that KRIT-1 protein is expressed in cultured arterial and venous endothelial cells and is present in cell–cell junctions. KRIT-1 colocalized and was physically associated with junctional proteins via its band 4.1/ezrin/radixin/moesin (FERM) domain. Rap1 activity regulated the junctional localization of KRIT-1 and its physical association with junction proteins. However, the association of the isolated KRIT-1 FERM domain was independent of Rap1. Small interfering RNA–mediated depletion of KRIT-1 blocked the ability of Rap1 to stabilize endothelial junctions associated with increased actin stress fibers. Thus, Rap1 increases KRIT-1 targeting to endothelial cell–cell junctions where it suppresses stress fibers and stabilizes junctional integrity.
Cerebral cavernous malformations (CCMs) are vascular malformations mostly found within the central nervous system. CCMs can occur in sporadic or autosomal dominant inherited forms, the latter of which map to three loci, KRIT-1/CCM1, MGC4607/OSM/CCM2, and PDCD10/CCM3 (Dubovsky et al., 1995; Craig et al., 1998; Labauge et al., 2007; Liquori et al., 2007). KRIT-1 protein was detected in endothelial cells by Western blot, immunofluorescence, and immunohistochemistry (Gunel et al., 2002; Guzeloglu-Kayisli et al., 2004a,b), but this work was challenged because KRIT-1 mRNA was not detected in the endothelium (Petit et al., 2006). However, CCM lesions are composed of endothelial cells (Wong et al., 2000; Clatterbuck et al., 2001) and can occur outside the brain (Eerola et al., 2000). Furthermore, mice lacking KRIT-1 die because of defective vascular development but have apparently normal brain development (Whitehead et al., 2004), all of which suggest that the primary defect in CCM lesions is in the endothelial compartment.
KRIT-1 possesses four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences, one of which is essential for integrin cytoplasmic domain-associated protein-1α (ICAP1α) binding (Zawistowski et al., 2002) and all of which mediate binding of CCM2 (Zawistowski et al., 2005; Zhang et al., 2007). KRIT-1 was identified as a Rap1-binding protein in yeast two hybrid experiments (Serebriiskii et al., 1997), and the FERM domain of KRIT-1 binds with 10-fold higher affinity to Rap1 than to H-Ras (Wohlgemuth et al., 2005). Although the interaction of Rap1 and full-length KRIT-1 has been disputed (Zhang et al., 2001), we have confirmed the association by coimmunoprecipitation (unpublished data). Rap1 regulates cell–cell junctions in both endothelial and epithelial cells (Knox and Brown, 2002; Price et al., 2004; Cullere et al., 2005). The disruption of endothelial cell junctions in CCM suggests that KRIT-1 may have a role in the capacity of Rap1 to regulate endothelial cell–cell junctions. Here, we report that KRIT-1 is expressed in endothelial cells where it is present in cell–cell junctions and associated with junctional proteins. The junctional localization of KRIT-1 is mediated by its FERM domain and regulated by Rap1 activity. Furthermore, we find that KRIT-1 is required for the stabilizing effect of Rap1 on endothelial cell–cell junctions. Together, these data establish that KRIT-1 is a Rap1-binding protein that regulates endothelial junction integrity and may provide a molecular explanation for aspects of the CCM phenotype.
Immunoprecipitation with an mAb anti–KRIT-1 (15B2) followed by Western blotting with an affinity-purified pAb (Rb6832) was performed to increase the sensitivity of detection of endogenous KRIT-1. We detected a band of ~80 kD in bovine aortic endothelial cells (BAECs; Fig. 1, A and B) and human umbilical vein endothelial cells (HUVECs; Fig. 1 B). The mobility of this band is consistent with the calculated molecular mass of KRIT-1 (81 kD). Moreover, CHO cells also exhibited this 80-kD band, and when transfected with authentic HA-tagged human KRIT-1 exhibited a second intense band of slightly lower mobility consistent with the presence of the HA tag (Fig. 1 A). An unrelated antibody (glyceraldehyde 3-phosphate dehydrogenase [GAPDH]) did not immunoprecipitate KRIT-1, nor did nonimmune mouse IgG. We used siRNA-mediated knockdown of endogenous KRIT-1 to confirm that the endogenous 80-kD polypeptide was authentic KRIT-1. Transfection with KRIT-1–specific siRNA (530; Fig. 1 B) completely eliminated this band from BAECs and reduced it by 70% in HUVECs, but had no effect on the expression of a variety of cell–cell junction proteins (Fig. 1 C). An irrelevant (Fig. 1 C, GAPDH) and a negative control siRNA (NC) had no effect on the expression of this polypeptide.
Having shown the antibodies' specificity in immunoprecipitation blotting experiments, we then used the mAb anti–KRIT-1 to detect endothelial cell KRIT-1 by immunofluorescence. KRIT-1 staining was enriched at sites of cell–cell junctions and the nucleus in confluent bovine aortic endothelial monolayers (Fig. 2 A), but was absent from free membranes (not depicted), suggesting cell junction localization consistent with the reported localization of recombinant KRIT-1 in transfected HEK293 cells (Zawistowski et al., 2005). Staining specificity was confirmed by its marked reduction after transfection with the KRIT-1 siRNA 530 (Fig. 2 A). Similar staining was seen in HUVECs and was also observed with the affinity-purified pAb anti–KRIT-1. No staining was seen with nonimmune rabbit or mouse IgG (unpublished data). Thus, KRIT-1 protein is expressed in both arterial and venous endothelial cells in culture, which is consistent with its reported presence in endothelium in vivo (Guzeloglu-Kayisli et al., 2004a).
Confocal microscopy demonstrated that KRIT-1 staining was enriched in cell junctions and colocalized with β-catenin, a cell–cell junction protein (Fig. 2 A; Vestweber, 2000). In KRIT-1 siRNA 530–treated cells, β-catenin localization to the cell–cell junction was disrupted but global expression of junctional proteins was unchanged (Figs. 1 C and 2 A). β-catenin also physically associated with KRIT-1, as did p120-catenin and the junctional scaffold AF-6 (also called afadin; Miyahara et al., 2000), as judged by their presence in KRIT-1 immunoprecipitates (Fig. 2 B). The coimmunoprecipitation was done under conditions that garnered near complete recovery of KRIT-1 from the cell lysate. From this, we estimate that KRIT associates with ~2% of total cellular β-catenin and p120-catenin and 6% of total AF-6. Nevertheless, the intensities of the KRIT-1 bands were similar to those observed for the junctional proteins, suggesting that KRIT-1 is not an abundant protein. This idea is supported by the requirement for prior immunoprecipitation to visualize KRIT-1 by immunoblotting (Fig. 2 B). The association of KRIT-1 with AF-6 is noteworthy as AF-6 can inhibit Rap1 activity (Su et al., 2003; Zhang et al., 2005) through recruitment of Rap GTPase activating proteins (GAPs). Thus, the interaction of KRIT-1 with AF-6 could act as a negative feedback modulator of Rap activity. Also present in coprecipitates, but not shown here, were vascular endothelial cadherin and α-catenin. In contrast, ZO-1, a tight junction marker, and talin, a focal adhesion protein absent from adherens junctions (Geiger et al., 1985), were not detected in KRIT-1 immunoprecipitates (Fig. 2 B).
KRIT-1 associates with Rap1 small GTPase and exhibits a preference for Rap versus Ras in vitro (Serebriiskii et al., 1997; Wohlgemuth et al., 2005). We therefore asked whether Rap1 could regulate the localization of KRIT-1 to the junctions and its association with junctional proteins. Transfection of cells with an activated Rap1 (Rap1A-G12V, referred to as RapV12) increased the association of endogenous KRIT-1 with β-catenin and AF-6 (Fig. 3 A). Expression of the inhibitor of Rap activity (Rap1GAP) inhibited the association of KRIT-1 with β-catenin and AF-6 to a greater extent than the increase elicited by activated Rap1A. The strong effect of Rap1GAP suggests the presence of basal Rap activity in BAECs. Neither RapV12 nor Rap1GAP expression affected KRIT-1 expression (Fig. 3 A). Furthermore, thrombin treatment led to a dramatic loss of KRIT-1 from the junctions (Fig. 3 B) but did not alter KRIT-1 expression (Fig. S1 A, available at http://www.jcb.org/cgi/content/full/200705175/DC1). Thrombin's effect was counteracted by the addition of an activator (Enserink et al., 2002) of the RapGEF Epac-1, 8-pCPT-2′-O-methyladenosine-3′,5′-cAMP (8-pCPT-2′-O-Me-cAMP; Fig. 3 B) . RapV12 also reversed the thrombin-stimulated loss of KRIT from the junctions, supporting the idea that effect of 8-pCPT-2′-O-Me-cAMP is caused by the activation of Rap1 and that KRIT-1 association with cell–cell junctions is increased by Rap1 activation.
To explore the mechanism of KRIT-1 targeting of endothelial cell–cell junctions, we examined the capacity of different domains of the protein to mediate junctional localization. The isolated KRIT-1 FERM domain (GFP-KRIT-F123; Fig. 4 A) colocalized with β-catenin in cell–cell junctions (Fig. 4 B). Furthermore, the KRIT-1 FERM domain was physically associated with β-catenin (Fig. 4 C). In sharp contrast to the intact protein, the interaction of GFP-KRIT-F123 with β-catenin was insensitive to the activation state of Rap1 (Fig. 4 D), suggesting that active Rap1 may increase the association of KRIT-1 with junctional proteins by increasing the availability of this domain. In contrast to the FERM domain, the KRIT-1 N terminus (Fig. 4 A) did not colocalize with either junctional β-catenin (Fig. 4 B) or ZO-1. Furthermore, the N terminus did not associate with β-catenin in coimmunoprecipitation experiments (Fig. 4 C). Moreover, the KRIT-F23 fragment of the FERM domain failed to localize to junctions, suggesting that the F1 region is required for localization to the junction. Thus, KRIT-1 localization to endothelial junctions is mediated by the KRIT-1 FERM domain and regulated by Rap1.
CCM lesions display characteristics typical of a loss of endothelial cell–cell junctions (Clatterbuck et al., 2001). As noted previously, KRIT-1 is a relatively specific Rap1 effector (Serebriiskii et al., 1997; Wohlgemuth et al., 2005), and Rap1 regulates the integrity of cell–cell junctions in endothelial and epithelial cells (Knox and Brown, 2002; Price et al., 2004; Cullere et al., 2005), leading us to examine the role of KRIT-1 in Rap1-induced stabilization of endothelial junctions. We noted that there was extensive redistribution of β-catenin out of cell–cell junctions in KRIT-1–depleted BAECs (Fig. 1 A), suggesting that KRIT-1 might be required for the stability of cell–cell junctions in these cells. As a test for the function of these junctions, we examined the permeability of endothelial cell monolayers to HRP (Stockton et al., 2004). KRIT-1 siRNA transfection caused an approximately twofold increase in permeability (Fig. 5 A) that was reversed by reconstitution with recombinant KRIT-1. Furthermore, treatment of KRIT-1–depleted cells with 8-pCPT-2′-O-Me-cAMP had little effect on permeability (Fig. 5 A). In sharp contrast, 8-pCPT-2′-O-Me-cAMP reversed the increase in permeability caused by thrombin as expected (Rangarajan et al., 2003; Cullere et al., 2005). Thrombin treatment caused a loss of KRIT-1 from the junctions (Fig. 3 B) and a concomitant redistribution of β-catenin away from the junction (Fig. S1 B); however, thrombin treatment did not disrupt the interaction of KRIT-1 and β-catenin, suggesting that they remain associated as they redistribute away from the junction (Fig. S1 A). Thrombin increased permeability further in KRIT-1–depleted cells. Furthermore, overexpression of KRIT-1 partially reversed the increased permeability induced by expression of Rap1GAP (Fig. 5 B). Thus, KRIT-1 regulates endothelial permeability and is required for Rap1-mediated stabilization of endothelial junctions.
We have shown here that Rap1 activation leads to association of KRIT-1 with endothelial cell–cell junction proteins. The KRIT-1 N terminus has binding sites for ICAP1α and CCM2 (Zawistowski et al., 2002, 2005; Zhang et al., 2007). Consequently, recruitment of KRIT-1 through its FERM domain has the potential to bring these proteins to the junction. Both ICAP1α and CCM2 are involved in the regulation of Rho family GTPases (Degani et al., 2002; Zawistowski et al., 2005), which are known to control the integrity of endothelial junctions via the actin cytoskeleton (Wojciak-Stothard and Ridley, 2002). Furthermore, activation of Rap1 by 8-pCPT-2′-O-Me-cAMP inhibits thrombin-induced RhoA activation in endothelial cells (Cullere et al., 2005). Hence, we examined F-actin distribution after depletion of KRIT-1 with siRNA. In control cells, actin was distributed around the circumference of each cell, and few stress fibers were observed. This contrasts strongly with KRIT-1 siRNA–treated cells, which had abundant stress fibers. Overexpression of HA-KRIT in control cells increased cortical actin staining, and HA-KRIT reexpression restored cortical actin morphology in KRIT-1 siRNA–treated cells (Fig. 5 C). As changes in actin morphology are linked to altered permeability (Stockton et al., 2004; Kooistra et al., 2005; Hayashi et al., 2006), this result suggests that KRIT-1 may act as a scaffold that regulates endothelial junctions by recruiting modulators of the Rho family GTPases that control the actin cytoskeleton.
CCM lesions are composed of a bed of leaky capillaries (Wong et al., 2000; Clatterbuck et al., 2001). Thus, our finding that the loss of KRIT-1 increases endothelial permeability provides a direct link between the pathogenesis of CCM and KRIT-1 function. KRIT-1 is a relatively specific effector of Rap1, which is a regulator of endothelial permeability and the stability of cell– cell junctions (Knox and Brown, 2002; Cullere et al., 2005). We now find that the capacity of Rap1 to stabilize endothelial cell junctions depends on KRIT-1 and that Rap1 regulates the junctional localization of the KRIT-1 protein and increases the association of KRIT-1 with junctional proteins. The KRIT-1 FERM domain mediates its association with junctional proteins and the isolated FERM domain associates with junctional proteins in a Rap1-independent manner. This suggests that the availability of the KRIT-1 FERM domain may be regulated by the binding of active Rap1. This would allow KRIT-1 to associate via the FERM domain with the junction complex, thus stabilizing cell–cell junctions, perhaps through modifying the activity of Rho family GTPases. In summary, Rap1 stimulates the junctional localization of KRIT-1 via KRIT-1's FERM domain, and KRIT-1 is required for Rap1 stabilization of endothelial junctions. These data provide a molecular linkage between KRIT-1 protein function and the CCM phenotype and identify a Rap effector that regulates cell–cell junctions.
BAECs were a gift of S. Shattil (University of California, San Diego, La Jolla, CA). BAECs were cultured in DME with 10% calf serum (CS) and 1% penicillin/streptomycin (Invitrogen). HUVECs were obtained from Cambrex and cultured in endothelial growth medium 2 (Cambrex). CHO cells were obtained from American Type Culture Collection. CHO cells were cultured in DME with 10% fetal bovine serum plus 1% penicillin/streptomycin, 1% l-glutamine, and 1% nonessential amino acids (Invitrogen).
siRNA directed against human KRIT was designed by Ambion. Three predesigned siRNA sequences (146530, 146531, and 214883) were tested and all were found to inhibit expression of KRIT protein in human cells. siRNA 146530 (530) was found to have activity in BAECs, as would be expected from its 100% identity in the target sequence, and was used in all subsequent experiments. Control siRNA was obtained from Ambion, including anti-GAPDH and negative control siRNA 1. All siRNA was used at equimolar concentrations.
cDNA encoding HA- and GFP-tagged full-length KRIT, a GFP-tagged KRIT FERM domain (GFP-F123), and a GFP-tagged FERM domain truncation (GFP-F23) was constructed from full-length KRIT-1 cDNA provided by H. Dietz (Johns Hopkins University, Baltimore, MD). Domain boundaries were estimated using protein sequence alignment with other FERM-containing proteins: KRIT-1 FERM (F123) extended from amino acids 410–737, F23 amino acids 512–737, and KRIT-1 N terminus amino acids 1–274. A GFP-tagged N-terminal truncation of KRIT was made by subcloning the N terminus from a GST construct into the pEGFP-C1 vector (CLONTECH Laboratories, Inc.). Mammalian expression constructs for HA-Rap1A-G12V (RapV12) and HA-Rap1GAP (RapGAP) were gifts from J. Bos (Utrecht University, Utrecht, Netherlands).
CHO cells were transfected with Lipofectamine Plus (Invitrogen) according to the manufacturer's instructions. Cells were plated at 80% confluence 24 h before transfection and media was replaced 3 h after transfection.
For Western blot detection of siRNA efficiency, siRNA was transfected into BAECs and HUVECs using HiPerfect transfection reagent (QIAGEN) according to the manufacturer's suggested instructions. Cells were plated at 60–70% confluence and 25 nM siRNA was incubated with the cells for 24–48 h with no difference in knockdown efficiency.
For permeability, immunofluorescence, and cotransfection studies, BAECs were transfected using a nucleofection device (Amaxa). In brief, 0.5 × 106 cells per transfection were suspended in Basic endothelial cell solution (Amaxa) together with 25 nM siRNA with or without 1 μg DNA. The cells were then nucleoporated using program M-003 (Amaxa). After recovery at 37°C for 10 min, the cells were plated as required. This method garnered transfection efficiencies from 70 to 90%.
Polyclonal anti–KRIT-1 6832 was developed against the recombinant KRIT-1 FERM (F123) domain coupled to keyhole limpet hemocyanin. Monoclonal anti–KRIT-1 antibodies were also developed using the recombinant KRIT-1 FERM (F123) domain as the antigen. Mice were immunized with the KRIT-1 FERM domain in incomplete Freund's adjuvant. Mouse sera were titered by ELISA against GST-F123 before fusion of splenic cells with myeloma cells. After fusion, single hybridoma cells were plated by limiting dilution, and antibody production was assayed in the hybridoma supernatant by ELISA. Titers were done in parallel on GST-coated plates to assess background binding. Hybridomas with high titer were selected for subcloning, and the process was repeated twice. Selected hybridoma supernatants were further purified by affinity chromatography on a protein G–Sepharose column.
For endogenous coimmunoprecipitation experiments, cells expressing KRIT-1 were scraped into 500 μl of lysis buffer containing 50 mM Tris, pH 7.4, 150 mM NaCl, 0.5% NP-40, and 5 mM MgCl2 plus a protease inhibitor cocktail (Roche). After resting 5 min on ice, the lysate was incubated for 15 min at 4°C with rocking. The lysate was spun down at 14,000 rpm for 10 min and the protein concentration of the supernatant was determined using a bicinchoninic acid assay (Pierce Chemical Co.). 500 μg of precleared total cell protein was added to 2 μg of immunoprecipitating antibody and incubated at 4°C with continuous rocking for 2 h. 10 μl of a 50% slurry of protein G–Sepharose beads (GE Healthcare) was then added and the rocking of the samples was continued overnight. The immunoprecipitated samples were washed three times with lysis buffer and solubilized with 10 μl SDS-PAGE sample buffer. Samples were resolved on 4–20% polyacrylamide gels (Invitrogen) in SDS-PAGE buffer and transferred to nitrocellulose membranes.
Anti–KRIT-1 15B2 was used for immunofluorescence at a dilution of 1:1,000. Rabbit anti-GFP (Invitrogen) was used at a dilution of 1:200 for immunoprecipitation of GFP-tagged proteins. Nonimmune mouse IgG was obtained from Santa Cruz Biotechnology, Inc. Rabbit anti–human–KRIT-1 6832 serum was used for immunoblotting at a dilution of 1:1,000. Mouse anti–human AF-6 (BD Biosciences), mouse anti–human ZO-1 (Zymed Laboratories), rabbit anti–human VE-cadherin (CD144; Serotec), mouse anti-murine p120-catenin (Sigma-Aldrich), and rabbit anti–human GAPDH (Santa Cruz Biotechnology, Inc.) were used for immunoblotting at dilutions of 1:1,000. Mouse anti–chicken β-catenin (6F9; Sigma-Aldrich), mouse anti-GFP (Invitrogen), and mouse anti–chicken talin (8d4; Sigma-Aldrich) were used for immunoblotting at dilutions of 1:5,000, 1:2,500, and 1:3,000, respectively. Anti–β-catenin was used in immunofluorescence at a dilution of 1:1,000. Blots were probed with the appropriate secondary antibody conjugated to Alexa 680 (Invitrogen) or IRDye 800 (Rockland Immunochemicals) and imaged using an infrared imaging system (Odyssey; Li-Cor). Blots were processed using Photoshop (Adobe) and all lanes were adjusted equally.
BAECs were grown to confluence on fibronectin (FN)-coated glass coverslips. Slips were fixed with 4% formaldehyde for 20 min, permeabilized for 10 min with 0.2% Triton X-100 (Sigma-Aldrich), and blocked for 1 h with 10% normal goat serum (NGS; Sigma-Aldrich) in PBS. Anti–β-catenin (1:1,000 in NGS) was added and incubated at RT for 1 h in a humidified chamber. Slips were washed three times with PBS plus 0.001% Triton X-100 (PBS-TX100), and then goat anti–mouse IgG Alexa 568, goat anti–mouse IgG Alexa 488, or goat anti–rabbit IgG Alexa 568 (Invitrogen) in NGS was added at 1:1,000 and incubated for 1 h at RT. Control stain was performed with second antibody only at 1:1,000. Slips were washed six times in PBS-TX100 and mounted on glass slides using 10 μl of fluorescent mounting medium (DakoCytomation) and dried overnight. Images were obtained using a confocal microscopy system (TCS SP2 AOBS; Leica DMRE microscope with an HCX PL APO 63× 1.32 oil objective). Images were processed using Photoshop. Colocalization images were created using ImageJ software (National Institutes of Health).
Concomitant to the leak assay, a portion of the transfected cells was plated on FN-coated glass coverslips and grown to confluence (~24 h) in low-glucose DME/10% CS. Cells were serum starved (0.5% CS) for 4 h then treated for 30 min with 100 μM 8-pCPT-2′-O-Me-cAMP or the vehicle alone (control), and indicated cells were treated with 2 U/ml thrombin for 60 min. Slips were fixed with 3.5% formaldehyde for 2 h, permeabilized for 10 min with 0.15% Triton X-100 and washed with TBST. Slips were blocked with 10% NGS for 60 min at RT and washed again. 200 μl/slip of primary antibody was incubated overnight at 4°C in a humidified chamber. The control stain was mouse IgG at 1:1,000. After washing, donkey anti–mouse IgG Alexa 488 (Invitrogen) was added at a 1:1,000 dilution overnight at 4°C. Coverslips were washed six times in alternating PBS/TBST rinses and mounted on 10 μl Prolong Gold mounting medium (Invitrogen) and photographed using a confocal microscopy system. Images were processed using Photoshop. For actin staining (Fig. 5 C), cells were incubated with a 1:200 dilution of Alexa 488 or Alexa 568–phalloidin (6 μM in methanol; Invitrogen) overnight at 4°C. Coverslips were washed and mounted as above and imaged using a microscope (DMLS; Lecia NPlan 40× 0.65 objective) with a camera (SPOT RT Color-2000; Diagnostic Instruments).
The permeability of the endothelial monolayer was evaluated using a leak assay originally described in Stockton et al. (2004). In brief, BAECs were grown to semiconfluence. Cells were transfected with 25 nM of negative control siRNA or KRIT-1 siRNA 530 with or without 1 μg pcDNA3.1 HA–KRIT-1. Transfected cells in phenol-free DME/10% CS were plated into 3-μm-pore polyester FN-coated transwell filters (Corning). Filter-plated cells were incubated for 48 h at 37°C to full confluence. Cells were then incubated in serum-free, phenol-free DME for 2 h. As indicated in the figure legends, in some conditions cells were treated with 100 μM 8-pCPT-2′-O-Me-cAMP for 30 min, and then half were treated with 2 U/ml thrombin (GE Healthcare) for 30 min. 50 μl of phenol-free DME containing 1.5 μg/ml HRP (Sigma-Aldrich) was added to upper wells for an additional 30 min. Filters were removed from outer wells and fixed in 3.5% formaldehyde and later stained with 0.25% Coomassie blue and examined by phase-contrast microscopy to reconfirm the integrity of cell monolayers.
The HRP content of the lower chamber medium was measured using a microplate peroxidase colorimetric assay. 100-μl-per-well guaiacol/sodium phosphate assay buffer (1:1) was added to 25 μl of each sample in triplicate. 25 μl of freshly made 0.6-mM H2O2 in ddH2O was added to each well for ~15 min or until color developed. Reaction was stopped with 10-μl-per-well 2N H2SO4. A490 was acquired and raw absorbance values were normalized as a percentage of control untransfected (vehicle) cell sample absorbance. Data was analyzed for statistical significance using analysis of variance and SigmaStat software (Jandel).
Fig. S1 shows the effect of thrombin treatment on KRIT-1 expression, association with β-catenin, and β-catenin localization. Online supplemental material is available at http://www.jcb.org/cgi/content/full/200705175/DC1.
This work was supported by grants from the National Institutes of Health (AR27214, HL 078784, and HL31950). A. Glading is a postdoctoral fellow of the American Cancer Society and J. Han was an arthritis investigator of the Arthritis Foundation.
A. Glading, J. Han, and R.A. Stockton contributed equally to this paper.
Dr. Han died on 4 July 2006.
Abbreviations used in this paper: 8-pCPT-2′-O-Me-cAMP, 8-pCPT-2′-O-methyladenosine-3′,5′-cAMP; BAEC, bovine aortic endothelial cell; CCM, cerebral cavernous malformation; CS, calf serum; FERM, band 4.1/ezrin/radixin/moesin; FN, fibronectin; GAP, GTPase activating protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HUVEC, human umbilical vein endothelial cell; ICAP1α, integrin cytoplasmic domain-associated protein-1α; NGS, normal goat serum.