PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of jcellbiolHomeThe Rockefeller University PressThis articleEditorsContactInstructions for AuthorsThis issue
 
From:
An external file that holds a picture, illustration, etc.
Object name is jcb1730349f07.jpg Object name is jcb1730349f07.jpg
Figure 7.
In the absence of Rei1, the cytoplasmic Arx1–Alb1 complex prevents the recycling of Tif6. (A) Disruptions of ARX1 or ALB1 compensate the cold-sensitive phenotype of a rei1Δ strain. Wild-type, rei1Δ, arx1Δ, or rei1Δ, arx1Δ strains were spotted on YPD medium in 10−1 dilution series (top); the same was performed for wild-type, rei1Δ, alb1Δ, or rei1Δ, alb1Δ strains (bottom). Cold-sensitive phenotypes were tested by incubating the plates for 3 d at 23°C. Generation times at 23°C were calculated from growth curves performed in liquid synthetic medium over a period of 30 h. The confidence intervals were calculated for a probability of 95%. (B) Disruptions of ARX1 or ALB1 compensate the Tif6 shuttling defect in a rei1Δ strain. The localization of Tif6-GFP was detected by epifluorescence microscopy in a wild-type, rei1Δ, and rei1Δ, alb1Δ or rei1Δ, arx1Δ double mutant strains.
Articles from The Journal of Cell Biology are provided here courtesy of
The Rockefeller University Press