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Figure 3.
Rei1 is functionally linked to the shuttling factors Arx1 and Tif6. (A) In the absence of Rei1, Arx1 and Tif6 are redistributed to the cytoplasm. The localization of Arx1-GFP, Tif6-GFP, or Rlp24-TAP was observed after the growth of rei1Δ or wild-type strains for 8 h in minimal medium at 23°C. (B) Rei1 is required for the binding of Arx1 to pre-60S complexes. rei1Δ or wild-type strains expressing ARX1-GFP or TIF6-GFP were grown for 8 h at 23°C, and whole cell extracts were prepared. The extracts were separated on sucrose gradients and analyzed as described in Fig. 1 C. The immunoblots, which were performed using antibodies against GFP, are displayed below the corresponding profile.
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