PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of brjcancerBJC HomepageBJC Advance online publicationBJC Current IssueSubmitting an article to BJCWeb feeds
 
Br J Cancer. 1998 October; 78(8): 1012–1017.
PMCID: PMC2063166

Comparison of benign and malignant follicular thyroid tumours by comparative genomic hybridization.

Abstract

DNA copy number changes were compared in 29 histologically benign follicular adenomas, of which five were atypical, and 13 follicular carcinomas of the thyroid by comparative genomic hybridization. DNA copy number changes were frequent in adenomas (14 out of 29, 48%). Most changes were gains, and they always involved a gain of the entire chromosome 7 (10 out of 29, 34%); other common gains involved chromosomes 5 (28%), 9 (10%), 12 (24%), 14 (21%), 17 (17%), 18 (14%) and X (17%). Losses were found only in four (14%) adenomas. Two of the five atypical adenomas had DNA copy number losses, and none had gains. Unlike adenomas, gains were rare and losses were frequent in carcinomas. A loss of chromosome 22 or 22q was particularly common in carcinomas (6 out of 13, 46%), whereas a loss of chromosome 22 was found in only two (7%) adenomas, one of which was atypical (P = 0.002). A loss of 1p was also frequent in carcinomas (31%), but gains of chromosomes 5, 7, 12, 14 or X that were common in adenomas were not found. Loss of chromosome 22 or 22q was present in six of the eight widely invasive follicular carcinomas, but in only one of the five minimally invasive carcinomas. We conclude that large DNA copy number changes are common in thyroid adenomas. These changes are strikingly different from those found in follicular carcinomas consisting of few losses and frequent gains, especially those of chromosome 7. A loss of chromosome 22 is common in widely invasive follicular carcinoma.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Belge G, Thode B, Rippe V, Bartnitzke S, Bullerdiek J. A characteristic sequence of trisomies starting with trisomy 7 in benign thyroid tumors. Hum Genet. 1994 Aug;94(2):198–202. [PubMed]
  • Björkqvist AM, Tammilehto L, Anttila S, Mattson K, Knuutila S. Recurrent DNA copy number changes in 1q, 4q, 6q, 9p, 13q, 14q and 22q detected by comparative genomic hybridization in malignant mesothelioma. Br J Cancer. 1997;75(4):523–527. [PMC free article] [PubMed]
  • Bondeson L, Bengtsson A, Bondeson AG, Dahlenfors R, Grimelius L, Wedell B, Mark J. Chromosome studies in thyroid neoplasia. Cancer. 1989 Aug 1;64(3):680–685. [PubMed]
  • Criado B, Barros A, Suijkerbuijk RF, Weghuis DO, Seruca R, Fonseca E, Castedo S. Detection of numerical alterations for chromosomes 7 and 12 in benign thyroid lesions by in situ hybridization. Histological implications. Am J Pathol. 1995 Jul;147(1):136–144. [PubMed]
  • el-Rifai W, Larramendy ML, Björkqvist AM, Hemmer S, Knuutila S. Optimization of comparative genomic hybridization using fluorochrome conjugated to dCTP and dUTP nucleotides. Lab Invest. 1997 Dec;77(6):699–700. [PubMed]
  • Grebe SK, McIver B, Hay ID, Wu PS, Maciel LM, Drabkin HA, Goellner JR, Grant CS, Jenkins RB, Eberhardt NL. Frequent loss of heterozygosity on chromosomes 3p and 17p without VHL or p53 mutations suggests involvement of unidentified tumor suppressor genes in follicular thyroid carcinoma. J Clin Endocrinol Metab. 1997 Nov;82(11):3684–3691. [PubMed]
  • Hedinger C, Williams ED, Sobin LH. The WHO histological classification of thyroid tumors: a commentary on the second edition. Cancer. 1989 Mar 1;63(5):908–911. [PubMed]
  • Herrmann MA, Hay ID, Bartelt DH, Jr, Ritland SR, Dahl RJ, Grant CS, Jenkins RB. Cytogenetic and molecular genetic studies of follicular and papillary thyroid cancers. J Clin Invest. 1991 Nov;88(5):1596–1604. [PMC free article] [PubMed]
  • Joensuu H, Klemi PJ. DNA aneuploidy in adenomas of endocrine organs. Am J Pathol. 1988 Jul;132(1):145–151. [PubMed]
  • Knuutila S, Björkqvist AM, Autio K, Tarkkanen M, Wolf M, Monni O, Szymanska J, Larramendy ML, Tapper J, Pere H, et al. DNA copy number amplifications in human neoplasms: review of comparative genomic hybridization studies. Am J Pathol. 1998 May;152(5):1107–1123. [PubMed]
  • Mohapatra G, Kim DH, Feuerstein BG. Detection of multiple gains and losses of genetic material in ten glioma cell lines by comparative genomic hybridization. Genes Chromosomes Cancer. 1995 Jun;13(2):86–93. [PubMed]
  • Roque L, Gomes P, Correia C, Soares P, Soares J, Castedo S. Thyroid nodular hyperplasia: chromosomal studies in 14 cases. Cancer Genet Cytogenet. 1993 Aug;69(1):31–34. [PubMed]
  • Roque L, Castedo S, Clode A, Soares J. Deletion of 3p25-->pter in a primary follicular thyroid carcinoma and its metastasis. Genes Chromosomes Cancer. 1993 Nov;8(3):199–203. [PubMed]
  • Ruttledge MH, Sarrazin J, Rangaratnam S, Phelan CM, Twist E, Merel P, Delattre O, Thomas G, Nordenskjöld M, Collins VP, et al. Evidence for the complete inactivation of the NF2 gene in the majority of sporadic meningiomas. Nat Genet. 1994 Feb;6(2):180–184. [PubMed]
  • Schofield DE, Beckwith JB, Sklar J. Loss of heterozygosity at chromosome regions 22q11-12 and 11p15.5 in renal rhabdoid tumors. Genes Chromosomes Cancer. 1996 Jan;15(1):10–17. [PubMed]
  • Teyssier JR, Liautaud-Roger F, Ferre D, Patey M, Dufer J. Chromosomal changes in thyroid tumors. Relation with DNA content, karyotypic features, and clinical data. Cancer Genet Cytogenet. 1990 Dec;50(2):249–263. [PubMed]
  • Tonk V, Osella P, Delasmorenas A, Wyandt HE, Milunsky A. Abnormalities of chromosome 22 in meningiomas and confirmation of the origin of a dicentric 22 by in situ hybridization. Cancer Genet Cytogenet. 1992 Nov;64(1):65–68. [PubMed]
  • van den Berg E, Oosterhuis JW, de Jong B, Buist J, Vos A, Dam A, Vermeij B. Cytogenetics of thyroid follicular adenomas. Cancer Genet Cytogenet. 1990 Feb;44(2):217–222. [PubMed]
  • van den Berg E, van Doormaal JJ, Oosterhuis JW, de Jong B, Wiersema J, Vos AM, Dam A, Vermeij A. Chromosomal aberrations in follicular thyroid carcinoma. Case report of a primary tumor and its metastasis. Cancer Genet Cytogenet. 1991 Jul 15;54(2):215–222. [PubMed]
  • WOOLNER LB, BEAHRS OH, BLACK BM, McCONAHEY WM, KEATING FR., Jr Classification and prognosis of thyroid carcinoma. A study of 885 cases observed in a thirty year period. Am J Surg. 1961 Sep;102:354–387. [PubMed]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK