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J Bacteriol. 1992 June; 174(12): 3993–3999.
PMCID: PMC206108

Cloning, sequencing, mapping, and transcriptional analysis of the groESL operon from Bacillus subtilis.


Using a gene probe of the Escherichia coli groEL gene, a 1.8-kb HindIII fragment of chromosomal DNA of Bacillus subtilis was cloned. Upstream sequences were isolated as a 3-kb PstI fragment. Sequencing of 2,525 bp revealed two open reading frames in the order groES groEL. Alignment of the GroES and GroEL proteins with those of eight other eubacteria revealed 50 to 65% and 72 to 84% sequence similarity, respectively. Primer extension studies revealed one potential transcription start site preceding the groESL operon (S) which was activated upon temperature upshift. Northern (RNA) analysis led to the detection of two mRNA species of 2.2 and 1.5 kb. RNA dot blot experiments revealed an at least 10-fold increase in the amount of specific mRNA from 0 to 5 min postinduction, remaining at this high level for 10 min and then decreasing. A 9-bp inverted repeat within the 5' leader region of the mRNA might be involved in regulation of the heat shock response. By using PBS1 transduction, the groESL operon was mapped at about 342 degrees.

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Selected References

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  • Arnosti DN, Singer VL, Chamberlin MJ. Characterization of heat shock in Bacillus subtilis. J Bacteriol. 1986 Dec;168(3):1243–1249. [PMC free article] [PubMed]
  • Baird PN, Hall LM, Coates AR. A major antigen from Mycobacterium tuberculosis which is homologous to the heat shock proteins groES from E. coli and the htpA gene product of Coxiella burneti. Nucleic Acids Res. 1988 Sep 26;16(18):9047–9047. [PMC free article] [PubMed]
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Carrascosa JL, Abella G, Marco S, Carazo JM. Three-dimensional reconstruction of the sevenfolded form of Bacillus subtilis Gro EL Chaperonin. J Struct Biol. 1990 Jul-Sep;104(1-3):2–8. [PubMed]
  • Carrascosa JL, García JA, Salas M. A protein similar to Escherichia coli gro EL is present in Bacillus subtilis. J Mol Biol. 1982 Jul 15;158(4):731–737. [PubMed]
  • Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. [PMC free article] [PubMed]
  • Chitnis PR, Nelson N. Molecular cloning of the genes encoding two chaperone proteins of the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem. 1991 Jan 5;266(1):58–65. [PubMed]
  • Dedonder RA, Lepesant JA, Lepesant-Kejzlarová J, Billault A, Steinmetz M, Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–993. [PMC free article] [PubMed]
  • Ellis RJ. The molecular chaperone concept. Semin Cell Biol. 1990 Feb;1(1):1–9. [PubMed]
  • Fayet O, Louarn JM, Georgopoulos C. Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes. Mol Gen Genet. 1986 Mar;202(3):435–445. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Ferrari FA, Nguyen A, Lang D, Hoch JA. Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol. 1983 Jun;154(3):1513–1515. [PMC free article] [PubMed]
  • Georgopoulos C, Ang D. The Escherichia coli groE chaperonins. Semin Cell Biol. 1990 Feb;1(1):19–25. [PubMed]
  • Guglielmi G, Mazodier P, Thompson CJ, Davies J. A survey of the heat shock response in four Streptomyces species reveals two groEL-like genes and three groEL-like proteins in Streptomyces albus. J Bacteriol. 1991 Nov;173(22):7374–7381. [PMC free article] [PubMed]
  • Hemmingsen SM, Woolford C, van der Vies SM, Tilly K, Dennis DT, Georgopoulos CP, Hendrix RW, Ellis RJ. Homologous plant and bacterial proteins chaperone oligomeric protein assembly. Nature. 1988 May 26;333(6171):330–334. [PubMed]
  • Hindersson P, Høiby N, Bangsborg J. Sequence analysis of the Legionella micdadei groELS operon. FEMS Microbiol Lett. 1991 Jan 1;61(1):31–38. [PubMed]
  • Hoch JA, Barat M, Anagnostopoulos C. Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967 Jun;93(6):1925–1937. [PMC free article] [PubMed]
  • Margolin W, Howe MM. Localization and DNA sequence analysis of the C gene of bacteriophage Mu, the positive regulator of Mu late transcription. Nucleic Acids Res. 1986 Jun 25;14(12):4881–4897. [PMC free article] [PubMed]
  • Mazodier P, Guglielmi G, Davies J, Thompson CJ. Characterization of the groEL-like genes in Streptomyces albus. J Bacteriol. 1991 Nov;173(22):7382–7386. [PMC free article] [PubMed]
  • Moran CP, Jr, Lang N, LeGrice SF, Lee G, Stephens M, Sonenshein AL, Pero J, Losick R. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982;186(3):339–346. [PubMed]
  • Morrison RP, Su H, Lyng K, Yuan Y. The Chlamydia trachomatis hyp operon is homologous to the groE stress response operon of Escherichia coli. Infect Immun. 1990 Aug;58(8):2701–2705. [PMC free article] [PubMed]
  • Narberhaus F, Bahl H. Cloning, sequencing, and molecular analysis of the groESL operon of Clostridium acetobutylicum. J Bacteriol. 1992 May;174(10):3282–3289. [PMC free article] [PubMed]
  • Narberhaus F, Giebeler K, Bahl H. Molecular characterization of the dnaK gene region of Clostridium acetobutylicum, including grpE, dnaJ, and a new heat shock gene. J Bacteriol. 1992 May;174(10):3290–3299. [PMC free article] [PubMed]
  • Parker J. Errors and alternatives in reading the universal genetic code. Microbiol Rev. 1989 Sep;53(3):273–298. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Shinnick TM, Vodkin MH, Williams JC. The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein. Infect Immun. 1988 Feb;56(2):446–451. [PMC free article] [PubMed]
  • Tilly K, Murialdo H, Georgopoulos C. Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1629–1633. [PubMed]
  • Webb R, Reddy KJ, Sherman LA. Regulation and sequence of the Synechococcus sp. strain PCC 7942 groESL operon, encoding a cyanobacterial chaperonin. J Bacteriol. 1990 Sep;172(9):5079–5088. [PMC free article] [PubMed]
  • Wetzstein M, Schumann W. Promoters of major Escherichia coli heat shock genes seem non-functional in Bacillus subtilis. FEMS Microbiol Lett. 1990 Oct;60(1-2):55–58. [PubMed]
  • Wetzstein M, Völker U, Dedio J, Löbau S, Zuber U, Schiesswohl M, Herget C, Hecker M, Schumann W. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J Bacteriol. 1992 May;174(10):3300–3310. [PMC free article] [PubMed]
  • Wood HE, Dawson MT, Devine KM, McConnell DJ. Characterization of PBSX, a defective prophage of Bacillus subtilis. J Bacteriol. 1990 May;172(5):2667–2674. [PMC free article] [PubMed]
  • Vodkin MH, Williams JC. A heat shock operon in Coxiella burnetti produces a major antigen homologous to a protein in both mycobacteria and Escherichia coli. J Bacteriol. 1988 Mar;170(3):1227–1234. [PMC free article] [PubMed]
  • Zeilstra-Ryalls J, Fayet O, Georgopoulos C. The universally conserved GroE (Hsp60) chaperonins. Annu Rev Microbiol. 1991;45:301–325. [PubMed]
  • Zuber U, Schumann W. Tn5cos: a transposon for restriction mapping of large plasmids using phage lambda terminase. Gene. 1991 Jul 15;103(1):69–72. [PubMed]

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