Search tips
Search criteria 


Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1993 August; 175(15): 4712–4718.
PMCID: PMC204922

Superinfection exclusion (sieB) genes of bacteriophages P22 and lambda.


The superinfection exclusion gene (sieB) of Salmonella phage P22 was mapped with phage deletion mutants. The DNA sequence in the region was reexamined in order to find an open reading frame consistent with the deletion mapping. Several discrepancies with the previously published sequence were discovered. The revised sequence revealed a single open reading frame of 242 codons with six likely translation initiation codons. On the basis of deletion and amber mutant phenotypes, the second of these six sites was inferred to be the translation initiation site of the sieB gene. The sieB gene encodes a polypeptide with 192 amino acid residues with a calculated molecular weight of 22,442, which is in reasonable agreement with that estimated from polyacrylamide gels. The transcription start site of sieB was identified by the use of an RNase protection assay. The sieB promoter thus identified was inactivated by a 2-base substitution in its -10 hexamer. The sieB gene of coliphage lambda was also identified. The promoter for lambda sieB was identified by homology to that of P22 sieB.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Benzer S. FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):344–354. [PubMed]
  • Berget PB, Poteete AR, Sauer RT. Control of phage P22 tail protein expression by transcription termination. J Mol Biol. 1983 Mar 15;164(4):561–572. [PubMed]
  • Berk AJ. Characterization of RNA molecules by S1 nuclease analysis. Methods Enzymol. 1989;180:334–347. [PubMed]
  • Brent R, Ptashne M. Mechanism of action of the lexA gene product. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4204–4208. [PubMed]
  • Calos MP. DNA sequence for a low-level promoter of the lac repressor gene and an 'up' promoter mutation. Nature. 1978 Aug 24;274(5673):762–765. [PubMed]
  • Franklin NC. Conservation of genome form but not sequence in the transcription antitermination determinants of bacteriophages lambda, phi 21 and P22. J Mol Biol. 1985 Jan 5;181(1):75–84. [PubMed]
  • Ineichen K, Shepherd JC, Bickle TA. The DNA sequence of the phage lambda genome between PL and the gene bet. Nucleic Acids Res. 1981 Sep 25;9(18):4639–4653. [PMC free article] [PubMed]
  • Kunkel TA, Roberts JD, Zakour RA. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987;154:367–382. [PubMed]
  • Moyle H, Waldburger C, Susskind MM. Hierarchies of base pair preferences in the P22 ant promoter. J Bacteriol. 1991 Mar;173(6):1944–1950. [PMC free article] [PubMed]
  • Poteete AR. Location and sequence of the erf gene of phage P22. Virology. 1982 Jun;119(2):422–429. [PubMed]
  • Rennell D, Bouvier SE, Hardy LW, Poteete AR. Systematic mutation of bacteriophage T4 lysozyme. J Mol Biol. 1991 Nov 5;222(1):67–88. [PubMed]
  • Sancar A, Rupert CS. Determination of plasmid molecular weights from ultraviolet sensitivities. Nature. 1978 Mar 30;272(5652):471–472. [PubMed]
  • Sanger F, Coulson AR, Hong GF, Hill DF, Petersen GB. Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982 Dec 25;162(4):729–773. [PubMed]
  • Schägger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987 Nov 1;166(2):368–379. [PubMed]
  • Semerjian AV, Malloy DC, Poteete AR. Genetic structure of the bacteriophage P22 PL operon. J Mol Biol. 1989 May 5;207(1):1–13. [PubMed]
  • Susskind MM, Botstein D, Wright A. Superinfection exclusion by P22 prophage in lysogens of Salmonella typhimurium. III. Failure of superinfecting phage DNA to enter sieA+ lysogens. Virology. 1974 Dec;62(2):350–366. [PubMed]
  • Susskind MM, Botstein D. Molecular genetics of bacteriophage P22. Microbiol Rev. 1978 Jun;42(2):385–413. [PMC free article] [PubMed]
  • Susskind MM, Botstein D. Superinfection exclusion by lambda prophage in lysogens of Salmonella typhimurium. Virology. 1980 Jan 15;100(1):212–216. [PubMed]
  • Susskind MM, Wright A, Botstein D. Superinfection exclusion by P22 prophage in lysogens of Salmonella typhimurium. IV. Genetics and physiology of sieB exclusion. Virology. 1974 Dec;62(2):367–384. [PubMed]
  • Weinstock GM, Susskind MM, Botstein D. Regional specificity of illegitimate recombination by the translocatable ampicillin-resistance element Tn1 in the genome of phage P22. Genetics. 1979 Jul;92(3):685–710. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)