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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Gene. Author manuscript; available in PMC 2008 October 15.
Published in final edited form as:
PMCID: PMC2049010

Structural characteristics of zebrafish orthologs of adaptor molecules that associate with transmembrane immune receptors


Transmembrane bound receptors comprised of extracellular immunoglobulin (Ig) or lectin domains play integral roles in a large number of immune functions including inhibitory and activating responses. The function of many of the activating receptors requires a physical interaction with an adaptor protein possessing a cytoplasmic regulatory motif. The partnering of an activating receptor with an adaptor protein relies on complementary charged residues in the two transmembrane domains. The mammalian natural killer (NK) and Fc receptors (FcR) represent two of many receptor families, which possess activating receptors that partner with adaptor proteins for signaling. Zebrafish represent a powerful experimental model for understanding developmental regulation at early stages of embryogenesis and for efficiently generating transgenic animals. In an effort to understand developmental aspects of immune receptor function, we have accessed the partially annotated zebrafish genome to identify six different adaptor molecules: Dap10, Dap12, Cd3ζ, Cd3ζ-like, FcRγ and FcRγ-like that are homologous to those effecting immune function in mammals. Their genomic organizations have been characterized, cDNA transcripts have been recovered, phylogenetic relationships have been defined and their cell lineage-specific expression patterns have been established.

Keywords: DAP10, DAP12, FcRγ, CD3ζ

1. Introduction

The cells of the mammalian immune system rely on an intricate network of signaling pathways in order to differentiate between “self” and “non-self”. The activation or inhibition of these signaling pathways relies on specific membrane receptors on immune cells engaging specific ligands. In general, these receptors can be classified as inhibitory or activating based on the functional outcome of ligand recognition. For example, when an activating natural killer (NK) cell receptor binds its ligand, the NK cell is activated to kill the target cell; in contrast, when an inhibitory NK receptor binds its ligand, NK cell-mediated killing is repressed. Similarly, engagement of T-cell antigen receptor (TCR) or Fc receptor (FcεRI) with the appropriate ligand (e.g. peptide-MHC complex or IgE, respectively) leads to a direct cell-mediated immune response.

Despite differences in receptor structures, the cytoplasmic signaling utilized by NK receptors, TCR and Fc receptors, are well conserved (Billadeau and Leibson 2002; Cerwenka and Lanier 2000; Yokoyama and Kim 2006). Inhibitory NK and Fc receptors typically possess one or more cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs). In contrast, activating NK, Fc and other receptors including TCR partner with adaptor proteins, which ultimately transduce signals to the cell nucleus. These receptors physically associate with an adaptor protein via oppositely charged residues within their transmembrane domains, e.g. a positive charge in the transmembrane domain of the activating receptor and a negative charge in the transmembrane domain of the adaptor protein. The majority of activating NK receptors, including most KIRs and Ly49, utilize the adaptor protein, DAP12; the activating NK receptor NKG2D, another lectin-type receptor, utilizes the adaptor DAP10 (Hyka-Nouspikel and Phillips 2006; Takaki et al 2006). TCR and FcR along with other immune related activating receptors, including: CD16, NKp30, NKp46 and NKR-P1C and KIR2DL4, utilize FcRγ or CD3ζ (Cerwenka and Lanier 2000; Tassi et al 2006). The DAP12, FcRγ and CD3ζ adaptors utilize cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs: YxxLX6-12YxxL/I) for signaling (Pitcher and van Oers 2003), whereas DAP10 uses a YxxM motif similar to CD28 (Wu et al 1999).

The zebrafish is becoming a more broadly recognized model for infection and immunity and is particularly well suited for examining gene function during embryogenesis (Deiters and Yoder 2006; Lieschke and Currie 2007; Phelps and Neely 2005; Traver et al 2003a; Trede et al 2004; Van Der Sar et al 2004; Yoder et al 2002). As part of an ongoing effort to characterize immune receptors in zebrafish and to understand the signaling pathways mediated by their immune cells, we have identified and characterized the adaptor molecules: Dap12, Dap10, CD3ζ, CD3ζ-like, FcRγ, and FcRγ-like.

2. Materials and methods

2.1 Cloning zebrafish dap10 (hcst) cDNA

A catfish (Ictalurus punctatus) DAP10 cDNA sequence (GenBank: AAZ16504) was used as the query for a tBLASTn search of the NCBI zebrafish sequence database (Wheeler et al 2007). Zebrafish bacterial artificial chromosome (BAC) clone DKEY-29H14 (GenBank: BX571853) from chromosome 16 encodes a sequence similar to catfish DAP10 (E value = 1e-10). A sequential RACE strategy was used to clone the full-length open reading frame (ORF) of dap10 (hcst) from zebrafish kidney/spleen RNA (GeneRacer, Invitrogen). Initially, 3′ RACE was completed with nested forward primers (designed from the DKEY-29H14 sequence) ZFDAP10-F1 and ZFDAP10-F2 which identified the 3′ untranslated region of the dap10 mRNA. Subsequently, nested, reverse primers ZFDAP10-3′UTR-R1 and ZFDAP10-3′UTR-R2 were designed within the 3′ UTR of dap10 and 5′ RACE was completed to generate a cDNA encoding the ORF of dap10. Primer sequences are listed in Table 1. PCR products were cloned into pGEM-T easy (Promega) or pCR4-TOPO (Invitrogen) and sequenced.

Table 1
Oligonucleotide Primer Sequence

2.2 Cloning zebrafish dap12 (tyrobp) cDNA

tBLASTn analyses of the zebrafish nucleotide (nr), genome and EST databases, using human DAP12 as a query, failed to identify a similar sequence (no sequence identified with an E value <0.05). As DAP10 and DAP12 are adjacent genes in mammals and pufferfish (Guselnikov et al 2003b), the zebrafish BAC DKEY-29H14, which encodes dap10, was examined for dap12 sequence. A BLASTx analysis using the nucleotide sequence of DKEY-29H14 as a query identified a short sequence (108 nucleotides) within this BAC with extremely low similarity (E value = 1.5) to the transmembrane domain of mouse TCRδ (GenBank: AAH62807) which, like the transmembrane domain of DAP12, includes a negatively charged residue. We predicted that this novel transmembrane domain encoded by BAC DKEY-29H14 would represent an exon of zebrafish dap12 (tyrobp) based on the following observations: 1) the transmembrane domains encoded by this novel zebrafish sequence and mammalian DAP12 include a negatively charged residue; 2) this candidate zebrafish transmembrane domain is adjacent to dap10; and 3) no other sequences within DKEY-29H14 shares similarity with DAP12. Subsequently, a full-length open reading frame cDNA of this sequence was cloned (as follows) and confirmed to encode dap12. Initially, 3′ RACE was completed from zebrafish kidney/spleen RNA with nested forward primers (designed from the DKEY-29H14 novel transmembrane sequence) ZFDAP12-F1 and ZFDAP12-F2 which identified the 3′ untranslated region of the dap12 mRNA. Subsequently, nested, reverse primers ZFDAP12-R3 and ZFDAP12-R4 were designed within the ORF of dap12 and 5′ RACE was completed to generate a cDNA encoding the 5′ UTR of dap12. Finally, nested, forward primers ZFDAP12-5′UTR-F3 and ZFDAP12-5′UTR-F4 were designed within the 5′ UTR of dap12 and a cDNA encoding the ORF of dap12 was derived by 3′ RACE. Primer sequences are listed in Table 1. PCR products were cloned into pGEM-T easy (Promega) or pCR4-TOPO (Invitrogen) and sequenced.

2.3 Cloning zebrafish FcRγ (fcer1g) cDNA

A zebrafish kidney EST (GenBank BG799671), which encodes FcRγ (fcer1g), was identified from a tBLASTn search using a catfish FcRγ sequence (GenBank AF538721) as a query. In order to confirm this sequence, a 5′ RACE strategy was performed as described above using spleen RNA and the nested reverse primers, ZFFcRg-3′UTR-R1 and ZFFcRg-3′UTR-R2, which are complementary to the 3′ UTR of FcRγ. Primer sequences are listed in Table 1. PCR products were cloned into pGEM-T easy (Promega) or pCR4-TOPO (Invitrogen) and sequenced.

2.4 Cloning zebrafish FcRγ-like (fcer1gl) cDNA

A zebrafish FcRγ-like (fcer1gl) EST sequence was identified (GenBank BC124700) from a tBLASTn search using catfish FcRγ-like sequence (GenBank AAN38001: Shen et al 2003) as a query. As this zebrafish EST encoded the entire open reading frame (ORF) of FcRγ-like, forward and reverse primers, ZFFcRg-B-F, and ZFFcRg-B-R, were designed to amplify the entire ORF from zebrafish spleen cDNA. Primer sequences are listed in Table 1. The FcRγ-like ORF was amplified from zebrafish spleen cDNA using 40 cycles and cloned into pCRII-TOPO (Invitrogen) and sequenced.

2.5 Cloning zebrafish cd3ζ (cd247) cDNA

A zebrafish cd3ζ (cd247) EST sequence was identified (GenBank EE715400) from a tBLASTn search using a trout CD3ζ sequence (GenBank CA344798; Rexroad, III et al 2003) as a query. As this zebrafish EST encoded the entire open reading frame (ORF) of cd3ζ, forward and reverse primers, ZFNEWCd3z-F, and ZFNEWCd3z-R, were designed to amplify the entire ORF from zebrafish spleen cDNA. Primer sequences are listed in Table 1. The cd3ζ ORF was amplified from zebrafish lymphocyte cDNA using 40 cycles and annealing at 70° C and subsequently cloned into pCRII-TOPO (Invitrogen) and sequenced.

2.6 Cloning zebrafish cd3ζ-like (cd247l) cDNA

A predicted zebrafish cd3ζ-like (cd247l) sequence was identified (GenBank XM_688285) from a tBLASTn search using the catfish CD3ζ-like sequence (GenBank AAZ16505) as a query. The same RACE strategy as described above for dap10 and dap12 was used to clone the full-length open reading frame of cd3ζ-like from zebrafish spleen RNA. Initially, 3′ RACE was completed with nested forward primers (complementary to a conserved cytoplasmic immunoreceptor tyrosine-based activation motif [ITAM]) ZFCD3z-F1 and ZFCD3z-F2 which confirmed the 3′ untranslated region of the cd3ζ-like mRNA. Subsequently, nested, reverse primers ZFCD3z-3′UTR-R1 and ZFCD3z-3′UTR-R2 were designed within the 3′ UTR of cd3ζ-like and 5′ RACE was completed to generate a cDNA encoding the ORF of cd3ζ-like. Primer sequences are listed in Table 1. PCR products were cloned into pGEM-T easy (Promega) or pCR4-TOPO (Invitrogen) and sequenced.

2.7 Phylogenetic analyses

Predicted leader and transmembrane domains of protein sequences were identified with SMART software (; Letunic et al 2004) and proteins were aligned by ClustalW (; Chenna et al 2003). Neighbor-joining trees (Saitou and Nei 1987) were constructed from pairwise Poisson correction distances with 2000 bootstrap replications by MEGA2.1 software (Kumar et al 2001). Sequences used for this alignment and analyses are listed in Table 2.

Table 2
Protein sequences used for phylogenetic comparisons

2.8 Reverse transcriptase-polymerase chain reaction

Zebrafish (AB strain) embryos were collected by natural mating and maintained at 28° C as described (Westerfield 2000). Tissues from adult zebrafish were dissected and myeloid and lymphoid cell lineages were purified from anterior kidney by cell sorting using forward and side scatter as described (Traver et al 2003b) and cDNAs were generated as described (Panagos et al 2006). One μl of cDNA was subjected to thermal cycling with gene-specific primers (see RT-PCR primers in Table 1) and Titanium Taq DNA polymerase (BD Biosciences) in a 20 μl PCR reaction and 10 μl were analyzed by agarose gel electrophoresis. The number of PCR cycles used for detecting dap10, dap12, FcRγ, FcRγ-like, Cd3ζ, Cd3ζ-like, and actin transcripts were 30 (annealing at 65° C), 40 (annealing at 65° C), 40 (annealing at 65° C), 30 (annealing at 70° C), 40 (annealing at 70° C), 40 (annealing at 65° C), and 25 (annealing at 65° C), respectively. Myeloperoxidase (mpx) transcripts were detected using 25 PCR cycles (annealing at 70° C) and TCRα transcripts were detected using 40 PCR cycles (annealing at 60° C). Forward and reverse primers span at least 1 intron and are listed in Table 1.

3. Results and Discussion

3.1 Zebrafish encode six adaptor proteins: Dap10, Dap12, FcRγ, FcRγ-like, Cd3ζ and Cd3ζ-like

As a first step in characterizing immune signaling patterns in zebrafish, we data-mined the zebrafish genome and EST databases to identify sequences corresponding to six candidate adaptor proteins. Full-length cDNAs encoding these proteins termed, Dap12 (tyrobp), Dap10 (hcst), FcRγ (fcer1g), FcRγ-like (fcer1gl), Cd3ζ (cd247) and Cd3ζ-like (cd247l), were cloned by RACE or RT-PCR and sequenced. The predicted protein sequences for these zebrafish adaptors are shown aligned to orthologous proteins in Figure 1. All six zebrafish adaptors encode a peptide leader sequence, a transmembrane domain with a negatively charged amino acid (Asp) and a cytoplasmic tail with at least one activating motif.

Figure 1
Comparison of zebrafish and mammalian adaptor protein sequences

Zebrafish Dap12 is 33% identical (46% similar) to human DAP12 and possesses a single cytoplasmic ITAM (Figure 1A). The zebrafish Dap10 sequence identified here differs from a previously described Dap10 EST (GenBank BQ261988) by a single residue, is 26% identical to human DAP10 (40% similar) and possesses the cytoplasmic tyrosine motif, YMNV, which is similar to the YINM motif encoded by mammalian DAP10 (Figure 1B and Guselnikov et al 2003b). Although this is not an exact match to the mammalian DAP10 signaling motif, this same peptide sequence is encoded by a catfish DAP10 sequence (GenBank DQ114899) and a related sequence, YMNT, is encoded by DAP10 in Takifugu (Guselnikov et al 2003b). It is likely relevant that the YMNV motif encoded by zebrafish and catfish DAP10 is highly similar to the CD28 YMNM motif that, (like DAP10's YINM motif), when tyrosine phosphorylated, binds phosphatidylinositol 3-kinase (PI3K: Cai et al 1995; Prasad et al 1994). The original experiments that led to the definition of the YxxM motif as necessary for PI3K (p85) binding is based on studies using mutated CD28 motifs. When the fourth residue of this motif was mutated from an apolar methionine to a polar cysteine CD28 lost the ability to bind PI3K (Cai et al 1995; Prasad et al 1994). It might be that if this motif is mutated from YxxM to YxxV, (which is a more conserved substitution and matches the zebrafish and catfish DAP10 motif), PI3K binding would be maintained. These observations suggest that the zebrafish DAP10 sequence reported here may signal via PI3K.

Zebrafish FcRγ and FcRγ-like are 44% and 40% identical (72% and 61% similar) to human FcRγ, respectively, and each possesses a single cytoplasmic ITAM (Figure 1C). Zebrafish Cd3ζ and Cd3ζ-like are 34% and 29% identical (57% and 52% similar) to human CD3ζ, respectively. Zebrafish Cd3ζ and Cd3ζ-like possess two cytoplasmic ITAMs and Cd3ζ possesses an additional ITAM-like sequence (Figures 1D and 1E; Underhill and Goodridge 2007).

A phylogenetic analysis was performed in order to further characterize the relationships between all six zebrafish adaptor proteins and their respective orthologs (Figure 2A). The DAP10 and DAP12 proteins form one large family and the CD3ζ and FcRγ proteins form a second family, supporting the hypotheses that DAP10 and DAP12 are evolutionarily derived from the same progenitor gene and that, likewise, CD3ζ and FcRγ are likely derived from a single gene (Guselnikov et al 2003a). The observation that DAP10 from fish and Xenopus group with mammalian DAP12, rather than DAP10 was unexpected (Figure 2A). A second phylogenetic analysis focused only on DAP10 and DAP12 shows the expected grouping for DAP10/DAP12 across all species (Figure 2B), suggesting that the fine-scale organization of Figure 2A is not reliable: this is likely a result of the gaps introduced into the DAP10/DAP12 sequences required to align them with the longer CD3ζ/FcRγ sequences. Of these six adaptors, Dap12, Dap10, FcRγ and Cd3ζ are likely orthologs of their mammalian counterparts, whereas, FcRγL and Cd3ζL are likely paralogs of FcRγ and Cd3ζ, having arisen via a gene duplication event. This interpretation is strongly supported by evidence for a whole-genome duplication event in the lineage leading to the teleosts (Amores et al 1998; Woods et al 2005). In contrast, no evidence is seen for a second copy of either DAP10 or DAP12 in zebrafish (see Section 3.2 for additional discussion).

Figure 2
Phylogenetic analyses of adaptor proteins

Zebrafish Dap12 is most closely related to the mammalian DAP12 sequences and groups with these orthologs with ~53% confidence (Figure 2A). To our knowledge, the full-length DAP12 cDNA sequence reported here represents the first from any non-mammalian species although a similar (partial) sequence was predicted from the Takifugu genome that was shown to partner with human KIR2DS2 (Feng et al 2006; Guselnikov et al 2003b) in an in vitro assay. Although the DAP10 family is less conserved, all of the DAP10 proteins possess similar signaling motifs (Figure 1), supporting both this classification and related functions.

Zebrafish FcRγ and FcRγL group with all other FcRγ orthologs with 54% confidence (Figure 2A). A sequence comparison of fish and mammalian FcRγ and FcRγL sequences suggests that the carp cDNA, originally defined as FcRγ (Fujiki et al 2000), is less similar to FcRγ than other fish genes. A second carp FcRγ gene may exist (Figure 2A). Furthermore, this analysis suggests that of the two proposed catfish sequences designated FcRγ, an unpublished FcRγ sequence (GenBank AF538721), here identified as “FcRγ”, is more closely related to the mammalian sequences than to the published FcRγ sequence, here identified as “FcRγL” (Figures (Figures11 and and2A;2A; Shen et al 2003). The CD3ζ family of proteins from mammals, Xenopus, chicken, and multiple fish species cluster with 73% confidence, however, CD3ζL sequences are more divergent from the CD3ζ sequences and from each other (Figure 2A). The three CD3ζL sequences identified here from zebrafish, catfish and trout are not well conserved (Figures (Figures1E1E and and2A)2A) suggesting that less selective pressure has been encountered during the evolution of this gene. The FcRγL and CD3ζL genes may be restricted to bony fish.

Further support for the nomenclature for these genes is provided by BLASTp analyses of mammalian proteins using the peptide sequences of the zebrafish adaptors as queries. Although the similarity is not strong, the mammalian protein with the strongest similarity to zebrafish Dap10 is a primate DAP10 (Table 3) and much of this similarity is focused on the transmembrane domain and cytoplasmic signaling motif (Figure 1B). In contrast to DAP10, DAP12 is much more conserved between fish and mammals (Figures (Figures1A1A and and2),2), suggesting that there has been more selective pressure on DAP12. In addition, this analysis confirms that zebrafish Cd3ζ and Cd3ζL are most similar to mammalian CD3ζ and that zebrafish FcRγ and FcRγL are most similar to mammalian FcRγ. Zebrafish CD3ζ and FcRγ are more closely related to their mammalian orthologs than are CD3ζL and FcRγL (Table 3)

Table 3
Mammalian proteins most similar to zebrafish adaptor proteins

3.2 Genomic organization of zebrafish adaptor proteins

Zebrafish dap10 (hcst) and dap12 (tyrobp) are encoded by BAC DKEY-29H14 (GenBank BX571853) which maps to chromosome 16. Zebrafish FcRγ (fcer1g) is encoded by BAC CH211-286P15 (GenBank BX324220), which maps to chromosome 7 and FcRγL (fcer1gl) is encoded by a chromosome 2 contig (GenBank NW_001513019). Only a single exon of zebrafish cd3ζ (cd247) has been placed on a chromosome 1 contig (GenBank NW_001511947) whereas cd3ζL (cd247l) is encoded by BAC CH211-218D20 (GenBank BX294189), which maps to chromosome 9. The genomic organization of these genes is depicted in Figure 3. Zebrafish dap10 and dap12 are immediately adjacent genes as they are in mammals and Takifugu (Guselnikov et al 2003b).

Figure 3
Genomic organization of zebrafish adaptor genes

The presence of two paralogs for a single mammalian gene (e.g. CD3ζ and FcRγ) is not uncommon in zebrafish as much evidence indicates a whole genome duplication event occurred in the lineage leading to the teleosts (Amores et al 1998; Woods et al 2005). The zebrafish paralogs of FcRγ are present on chromosomes 7 and 2 which have been shown to encode other duplicated genes (e.g. ap1g1 and ap1g2). Similarly, the zebrafish paralogs of Cd3ζ are present on chromosomes 1 and 9 which encode other duplicated genes (e.g. dlx2a and dlx2b) and are predicted to be the result of an ancient chromosomal duplication (Woods et al 2005). In contrast the characterization of zebrafish dap10 and dap12 as single copy genes is supported by the estimate that only ~25% of the duplicated genes are currently identifiable within the zebrafish genome (Woods et al 2005). The completion of its genome will conclusively determine if zebrafish possess paralogs for dap10 and dap12.

DAP10 and DAP12 are tightly associated genes in mammals and zebrafish. They are immediately adjacent genes and separated by ~200 and ~ 8,700 bp in human and zebrafish, respectively. In contrast, FcRγ and Cd3ζ, which are encoded by the same chromosome in human and mice, are present on two chromosomes in zebrafish. This difference in physical linkage is likely due to the larger intergenic distance between these genes in mammals (~6,200,000 bp in human) and the extensive number of interchromosomal rearrangements since the ancestral genome of bony vertebrates (Woods et al 2005).

Further evidence supporting the identity of these zebrafish adaptors is provided by the syntenic relationships between the human and zebrafish loci for these genes. Four zebrafish genes which flank each adaptor gene (two genes on each side) were identified and the orthologous genes from human were defined by BLASTp analyses (Table 4). For example, zebrafish FcRγ (fcer1g) is adjacent to zgc:112036 which shares high identity with human NDUFS2: both human FcRγ (FCER1G) and NDUFS2 map to human chromosome 1q23. There is evidence for conserved synteny in all six adaptor gene loci in zebrafish: one or more of the genes which flank each zebrafish adaptor gene maps to the same chromosomal region as the human adaptor gene (Table 4).

Table 4
Evidence for conserved synteny at adaptor gene loci.1

3.3 Zebrafish adaptor proteins are differentially expressed in hematopoietic lineages

The expression of dap10, dap12, FcRγ, FcRγL, cd3ζ, and cd3ζL during zebrafish embryogenesis and in adult tissues (ovary, liver, kidney, spleen and intestine) (Figure 4A) was examined using an RT-PCR strategy. Maternal transcripts of all six adaptors can be detected in the ovary and dap10 transcripts continue to be detected in the 1-cell stage embryo (0 hours post fertilization, hpf). Zebrafish FcRγ is the only adaptor that is detected at later stages of embryogenesis (72 hpf) whereas FcRγ, cd3ζ, and cd3ζL transcripts are detected at the larval stage of 6 days post fertilization (dpf). This may reflect either very low levels of expression, expression in small sets of cells or simply a lack of expression at the early stages of development. Similar expression patterns of dap10, dap12, cd3ζL, FcRγ and FcRγL transcripts can be detected in adult liver, kidney, spleen and intestine, whereas Cd3ζ is primarily detected in ovary and spleen, with lower levels of expression in intestine. Analysis of adaptor expression in zebrafish lymphoid and myeloid cells was completed using flow cytometrically separated cell populations (Figure 4B) and reveals that dap10, dap12 and cd3ζ are restricted to lymphocytes whereas cd3ζL, FcRγ and FcRγL are expressed in both lymphoid and myeloid lineages (Figure 4C). Based on these distributions, it is likely that the adaptor homologs may function in immune signaling systems in zebrafish.

Figure 4
Detection of adaptor protein expression

3.4 Concluding remarks

As DAP12, DAP10, CD3ζ and FcRγ are all expressed by mammalian NK cells (Hamerman and Lanier 2006; Wu et al 2000), these data raise the possibility that zebrafish lymphocytes (purified by forward and side scatter) include NK cells. Although clonal NK-like cell lines have been described from catfish (Shen et al 2004), there are currently no reagents for identifying or purifying pure NK cell populations from bony fish. Finally, the observation that zebrafish dap10 and dap12 transcripts are not detected in myeloid cells was unexpected as the mammalian orthologs of these genes are expressed in both lymphoid and myeloid cells (Takaki et al 2006; Wu et al 1999): it is possible that dap10 and dap12 are expressed at very low levels or in a small population of cells within the zebrafish myeloid lineage.

The observation that Takifugu DAP12 can associate with a human KIR in a cell culture system suggests a conserved function for Dap12 (Feng et al 2006); however, KIRs have not been identified in bony fish. Other candidate activating NK receptors, that possess positively charged residues within their transmembrane domains, have been identified from zebrafish including an immunoglobulin-type receptor, Nitr9, and a lectin-type receptor, Illr3 (Panagos et al 2006; Yoder et al 2004): it will be of interest to determine if these receptors physically partner with Dap12. The presence of paralogs for CD3ζ and FcRγ in zebrafish raises additional questions of functional specificity. Do zebrafish TCR and FcεRI utilize a single adaptor for signaling? If so, which one? And what role does the second adaptor paralog play in immune response? Knowledge of the structures of the zebrafish adaptors and the relationships of these structures to each other will be useful for the design of specific reagents and engineering of constructs for examining the specific interactions and functions of these adaptor molecules in the immunological and developmental context of the zebrafish model.


We thank Melissa Haendel and John Hansen for very helpful discussions about gene nomenclature and Barb Pryor for editorial assistance. Zebrafish genes were named in consultation with the ZFIN Nomenclature Committee. Sequence data from this article have been deposited with the GenBank and ZFIN databases under accession numbers EF158445 and ZDB-GENE-061130-1 (Dap10/hcst); EF158446 and ZDB-GENE-061130-2 (Dap12/tyrobp); EF158447 and ZDB-GENE-061130-3 (FcRγ/fcer1g); EF601085 and ZDB-GENE-070502-4 (FcRγ-like/fcer1gl); EF601086 and ZDB-GENE-061130-4 (Cd3ζ/cd247); and EF158448 and ZDB-GENE-070508-2 (Cd3ζ-like/cd247l). This research was supported by NSF grant MCB-0505585 (JAY), NIH grant R01 AI057559 (GWL), and by funding from the All Children's Hospital Foundation (GWL).


Fc receptor
immunoreceptor tyrosine-based activation motif
immunoreceptor tyrosine-based inhibition motif
natural killer
T-cell antigen receptor


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  • Amores A, et al. Zebrafish hox clusters and vertebrate genome evolution. Science. 1998;282:1711–1714. [PubMed]
  • Billadeau DD, Leibson PJ. ITAMs versus ITIMs: striking a balance during cell regulation. J. Clin. Invest. 2002;109:161–168. [PMC free article] [PubMed]
  • Cai YC, Cefai D, Schneider H, Raab M, Nabavi N, Rudd CE. Selective CD28pYMNM mutations implicate phosphatidylinositol 3-kinase in CD86-CD28-mediated costimulation. Immunity. 1995;3:417–426. [PubMed]
  • Cerwenka A, Lanier LL. Natural killer cells, viruses and cancer. Nature Reviews. 2000;1:41–49. [PubMed]
  • Chang C, Dietrich J, Harpur AG, Lindquist JA, Haude A, Loke YW, King A, Colonna M, Trowsdale J, Wilson MJ. Cutting edge: KAP10, a novel transmembrane adapter protein genetically linked to DAP12 but with unique signaling properties. J. Immunol. 1999;163:4651–4654. [PubMed]
  • Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD. Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 2003;31:3497–3500. [PMC free article] [PubMed]
  • Deiters A, Yoder JA. Conditional Transgene and Gene Targeting Methodologies in Zebrafish. Zebrafish. 2006;3:415–429. [PubMed]
  • Feng J, Call ME, Wucherpfennig KW. The assembly of diverse immune receptors is focused on a polar membrane-embedded interaction site. PLoS. Biol. 2006;4:e142. [PubMed]
  • Feng J, Garrity D, Call ME, Moffett H, Wucherpfennig KW. Convergence on a distinctive assembly mechanism by unrelated families of activating immune receptors. Immunity. 2005;22:427–438. [PMC free article] [PubMed]
  • Fujiki K, Shin DH, Nakao M, Yano T. Molecular cloning and expression analysis of carp (Cyprinus carpio) interleukin-1 beta, high affinity immunoglobulin E Fc receptor gamma subunit and serum amyloid A. Fish Shellfish. Immunol. 2000;10:229–242. [PubMed]
  • Gobel TW, Bolliger L. The chicken TCR zeta-chain restores the function of a mouse T cell hybridoma. J Immunol. 1998;160:1552–1554. [PubMed]
  • Guselnikov SV, Bell A, Najakshin AM, Robert J, Taranin AV. Signaling FcRgamma and TCRzeta subunit homologs in the amphibian Xenopus laevis. Dev. Comp Immunol. 2003a;27:727–733. [PubMed]
  • Guselnikov SV, Najakshin AM, Taranin AV. Fugu rubripes possesses genes for the entire set of the ITAM-bearing transmembrane signal subunits. Immunogenetics. 2003b;55:472–479. [PubMed]
  • Hamerman JA, Lanier LL. Inhibition of immune responses by ITAM-bearing receptors. Sci. STKE. 2006;2006:re1. [PubMed]
  • Hyka-Nouspikel N, Phillips JH. Physiological roles of murine DAP10 adapter protein in tumor immunity and autoimmunity. Immunol. Rev. 2006;214:106–117. [PubMed]
  • Kumar S, Tamura K, Jakobsen IB, Nei M. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics. 2001;17:1244–1245. [PubMed]
  • Kuster H, Thompson H, Kinet JP. Characterization and expression of the gene for the human Fc receptor gamma subunit. Definition of a new gene family. J. Biol. Chem. 1990;265:6448–6452. [PubMed]
  • Lanier LL, Corliss BC, Wu J, Leong C, Phillips JH. Immunoreceptor DAP12 bearing a tyrosine-based activation motif is invovled in activating NK cells. Nature. 1998;391:703–707. [PubMed]
  • Letunic I, Copley RR, Schmidt S, Ciccarelli FD, Doerks T, Schultz J, Ponting CP, Bork P. SMART 4.0: towards genomic data integration. Nucleic Acids Res. 2004;32(Database issue):D142–D144. [PMC free article] [PubMed]
  • Lieschke GJ, Currie PD. Animal models of human disease: zebrafish swim into view. Nat. Rev. Genet. 2007;8:353–367. [PubMed]
  • Panagos PG, Dobrinski KP, Chen X, Grant AW, Traver D, Djeu JY, Wei S, Yoder JA. Immune-related, lectin-like receptors are differentially expressed in the myeloid and lymphoid lineages of zebrafish. Immunogenetics. 2006;58:31–40. [PubMed]
  • Phelps HA, Neely MN. Evolution of the Zebrafish Model: From Development to Immunity and Infectious Disease. Zebrafish. 2005;2:87–103. [PubMed]
  • Pitcher LA, van Oers NS. T-cell receptor signal transmission: who gives an ITAM? Trends Immunol. 2003;24:554–560. [PubMed]
  • Prasad KV, Cai YC, Raab M, Duckworth B, Cantley L, Shoelson SE, Rudd CE. T-cell antigen CD28 interacts with the lipid kinase phosphatidylinositol 3-kinase by a cytoplasmic Tyr(P)-Met-Xaa-Met motif. Proc. Natl. Acad. Sci. U. S. A. 1994;91:2834–2838. [PubMed]
  • Ra C, Jouvin MH, Kinet JP. Complete structure of the mouse mast cell receptor for IgE (Fc epsilon RI) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells. J. Biol. Chem. 1989;264:15323–15327. [PubMed]
  • Rexroad CE, III, Lee Y, Keele JW, Karamycheva S, Brown G, Koop B, Gahr SA, Palti Y, Quackenbush J. Sequence analysis of a rainbow trout cDNA library and creation of a gene index. Cytogenet. Genome Res. 2003;102:347–354. [PubMed]
  • Rutledge T, Cosson P, Manolios N, Bonifacino JS, Klausner RD. Transmembrane helical interactions: zeta chain dimerization and functional association with the T cell antigen receptor. EMBO J. 1992;11:3245–3254. [PubMed]
  • Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 1987;4:406–425. [PubMed]
  • Shen L, Stuge TB, Bengten E, Wilson M, Chinchar VG, Naftel JP, Bernanke JM, Clem LW, Miller NW. Identification and characterization of clonal NK-like cells from channel catfish (Ictalurus punctatus) Dev. Comp Immunol. 2004;28:139–152. [PubMed]
  • Shen L, Stuge TB, Evenhuis JP, Bengten E, Wilson M, Chinchar VG, Clem LW, Miller NW. Channel catfish NK-like cells are armed with IgM via a putative FcμR. Dev. Comp Immunol. 2003;27:699–714. [PubMed]
  • Takaki R, Watson SR, Lanier LL. DAP12: an adapter protein with dual functionality. Immunol. Rev. 2006;214:118–129. [PubMed]
  • Tassi I, Klesney-Tait J, Colonna M. Dissecting natural killer cell activation pathways through analysis of genetic mutations in human and mouse. Immunol. Rev. 2006;214:92–105. [PubMed]
  • Traver D, Herbomel P, Patton EE, Murphey RD, Yoder JA, Litman GW, Catic A, Amemiya CT, Zon LI, Trede NS. The zebrafish as a model organism to study development of the immune system. Adv. Immunol. 2003a;81:253–330. [PubMed]
  • Traver D, Paw BH, Poss KD, Penberthy WT, Lin S, Zon LI. Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nat. Immunol. 2003b;4:1238–1246. [PubMed]
  • Trede NS, Langenau DM, Traver D, Look AT, Zon LI. The use of zebrafish to understand immunity. Immunity. 2004;20:367–379. [PubMed]
  • Underhill DM, Goodridge HS. The many faces of ITAMs. Trends Immunol. 2007;28:66–73. [PubMed]
  • Van Der Sar AM, Appelmelk BJ, Vandenbroucke-Grauls CM, Bitter W. A star with stripes: zebrafish as an infection model. Trends Microbiol. 2004;12:451–457. [PubMed]
  • Weissman AM, Baniyash M, Hou D, Samelson LE, Burgess WH, Klausner RD. Molecular cloning of the zeta chain of the T cell antigen receptor. Science. 1988a;239:1018–1021. [PubMed]
  • Weissman AM, Hou D, Orloff DG, Modi WS, Seuanez H, O'Brien SJ, Klausner RD. Molecular cloning and chromosomal localization of the human T-cell receptor zeta chain: distinction from the molecular CD3 complex. Proc. Natl. Acad. Sci. U. S. A. 1988b;85:9709–9713. [PubMed]
  • Westerfield M. Guide for the Laboratory Use of Zebrafish (Danio rerio) 4th edition University of Oregon Press; Eugene: 2000. The Zebrafish Book.
  • Wheeler DL, et al. Database resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2007;35:D5–12. [PMC free article] [PubMed]
  • Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS. The zebrafish gene map defines ancestral vertebrate chromosomes. Genome Res. 2005;15:1307–1314. [PubMed]
  • Wu J, Cherwinski H, Spies T, Phillips JH, Lanier LL. DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells. J. Exp. Med. 2000;192:1059–1068. [PMC free article] [PubMed]
  • Wu J, Song Y, Bakker ABH, Bauer S, Spies T, Lanier LL, Phillips JH. An activating immunoreceptor complex formed by NKG2D and DAP10. Science. 1999;285:703–732. [PubMed]
  • Yoder JA, et al. Resolution of the novel immune-type receptor gene cluster in zebrafish. Proc Natl Acad Sci U S A. 2004;101:15706–15711. [PubMed]
  • Yoder JA, Nielsen ME, Amemiya CT, Litman GW. Zebrafish as an immunological model system. Microbes and Infection. 2002;4:1469–1478. [PubMed]
  • Yokoyama WM, Kim S. Licensing of natural killer cells by self-major histocompatibility complex class I. Immunol. Rev. 2006;214:143–154. [PubMed]