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Appl Environ Microbiol. 1987 September; 53(9): 2159–2164.
PMCID: PMC204074

Na+-Stimulated Transport of l-Methionine in Brevibacterium linens CNRZ 918


The transport of l-methionine by the gram-positive species Brevibacterium linens CNRZ 918 is described. The one transport system (Km = 55 μM) found is constitutive for l-methionine, stereospecific, and pH and temperature dependent. Entry of l-methionine into cells is controlled by the internal methionine pool. Competition studies indicate that l-methionine and α-aminobutyric acid share a common carrier for their transport. Neither methionine derivatives substituted on the amino or carboxyl groups nor d-methionine was an inhibitor, whereas powerful inhibition was shown by l-cysteine, s-methyl-l-cysteine, dl-selenomethionine and dl-homocysteine. Sodium plays important and varied roles in l-methionine transport by B. linens CNRZ 918: (i) it stimulates transport without affecting the Km, (ii) it increases the specific activity (on a biomass basis) of the l-methionine transport system when present with methionine in the medium, suggesting a coinduction mechanism. l-Methionine transport requires an exogenous energy source, which may be succinic, lactic, acetic, or pyruvic acid but not glucose or sucrose. The fact that l-methionine transport was stimulated by potassium arsenate and to a lesser extent by potassium fluoride suggests that high-energy phosphorylated intermediates are not involved in the process. Monensin eliminates stimulation by sodium. Gramicidin and carbonyl cyanide-m-chlorophenylhydrazone act in the presence or absence of Na+. N-Ethylmaleimide, p-chloromercurobenzoate, valinomycin, sodium azide, and potassium cyanide have no or only a partial inhibitory effect. These results tend to indicate that the proton motive force reinforced by the Na+ gradient is involved in the mechanism of energy coupling of l-methionine transport by B. linens CNRZ 918. Thus, this transport is partially similar to the well-described systems in gram-negative bacteria, except for the role of sodium, which is very effective in B. linens, a species adapted to the high sodium levels of its niche.

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Selected References

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  • Ayling PD, Bridgeland ES. Methionine transport in wild-type and transport-defective mutants of Salmonella typhimurium. J Gen Microbiol. 1972 Nov;73(1):127–141. [PubMed]
  • Ayling PD, Mojica-a T, Klopotowski T. Methionine transport in Salmonella typhimurium: evidence for at least one low-affinity transport system. J Gen Microbiol. 1979 Oct;114(2):227–246. [PubMed]
  • Boyaval P, Moreira E, Desmazeaud MJ. Transport of aromatic amino acids by Brevibacterium linens. J Bacteriol. 1983 Sep;155(3):1123–1129. [PMC free article] [PubMed]
  • Boyaval P, Moreira E, Desmazeaud MJ. Electrochemical proton gradient of Brevibacterium linens and its relationship to phenylalanine transport. Ann Microbiol (Paris) 1984 Jul-Aug;135B(1):91–99. [PubMed]
  • Brown KD. Formation of aromatic amino acid pools in Escherichia coli K-12. J Bacteriol. 1970 Oct;104(1):177–188. [PMC free article] [PubMed]
  • Cooper S. Utilization of d-Methionine by Escherichia coli. J Bacteriol. 1966 Aug;92(2):328–332. [PMC free article] [PubMed]
  • Ferchichi M, Hemme D, Bouillanne C. Influence of Oxygen and pH on Methanethiol Production from l-Methionine by Brevibacterium linens CNRZ 918. Appl Environ Microbiol. 1986 Apr;51(4):725–729. [PMC free article] [PubMed]
  • Ferchichi M, Hemme D, Nardi M, Pamboukdjian N. Production of methanethiol from methionine by Brevibacterium linens CNRZ 918. J Gen Microbiol. 1985 Apr;131(4):715–723. [PubMed]
  • Kadner RJ. Transport systems for L-methionine in Escherichia coli. J Bacteriol. 1974 Jan;117(1):232–241. [PMC free article] [PubMed]
  • Kadner RJ. Regulation of methionine transport activity in Escherichia coli. J Bacteriol. 1975 Apr;122(1):110–119. [PMC free article] [PubMed]
  • Kadner RJ. Transport and utilization of D-methionine and other methionine sources in Escherichia coli. J Bacteriol. 1977 Jan;129(1):207–216. [PMC free article] [PubMed]
  • Kadner RJ, Watson WJ. Methionine transport in Escherichia coli: physiological and genetic evidence for two uptake systems. J Bacteriol. 1974 Aug;119(2):401–409. [PMC free article] [PubMed]
  • Kadner RJ, Winkler HH. Energy coupling for methionine transport in Escherichia coli. J Bacteriol. 1975 Sep;123(3):985–991. [PMC free article] [PubMed]
  • Kitada M, Horikoshi K. Sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species. J Bacteriol. 1977 Sep;131(3):784–788. [PMC free article] [PubMed]
  • Laakso S. The relationship between methionine uptake and demethiolation in a methionine-utilizing mutant of Pseudomonas fluorescens UK1. J Gen Microbiol. 1976 Aug;96(2):391–394. [PubMed]
  • Lanyi JK, Yearwood-Drayton V, MacDonald RE. Light-induced glutamate transport in Halobacterium halobium envelope vesicles. I. Kinetics of the light-dependent and the sodium-gradient-dependent uptake. Biochemistry. 1976 Apr 20;15(8):1595–1603. [PubMed]
  • Mäntsälä P, Laakso S, Nurmikko V. Observations on methionine transport in Pseudomonas fluorescens UK1. J Gen Microbiol. 1974 Sep;84(1):19–27. [PubMed]
  • Mitchell P. Vectorial chemistry and the molecular mechanics of chemiosmotic coupling: power transmission by proticity. Biochem Soc Trans. 1976;4(3):399–430. [PubMed]
  • Montie DB, Montie TC. Methionine transport in Yersinia pestis. J Bacteriol. 1975 Oct;124(1):296–306. [PMC free article] [PubMed]
  • Niiya S, Moriyama Y, Futai M, Tsuchiya T. Cation coupling to melibiose transport in Salmonella typhimurium. J Bacteriol. 1980 Oct;144(1):192–199. [PMC free article] [PubMed]
  • Schellenberg GD, Furlong CE. Resolution of the multiplicity of the glutamate and aspartate transport systems of Escherichia coli. J Biol Chem. 1977 Dec 25;252(24):9055–9064. [PubMed]
  • Shiio I, Miyajima R, Kashima N. Na+-dependent transport of threonine in Brevibacterium flavum. J Biochem. 1973 Jun;73(6):1185–1193. [PubMed]
  • Thompson J, MacLeod RA. Functions of Na+ and K+ in the active transport of -aminoisobutyric acid in a marine pseudomonad. J Biol Chem. 1971 Jun 25;246(12):4066–4074. [PubMed]
  • Tsuchiya T, Hasan SM, Raven J. Glutamate transport driven by an electrochemical gradient of sodium ions in Escherichia coli. J Bacteriol. 1977 Sep;131(3):848–853. [PMC free article] [PubMed]

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