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Appl Environ Microbiol. 1988 April; 54(4): 990–995.
PMCID: PMC202585

Lysis of Halobacteria in Bacto-Peptone by Bile Acids

Abstract

All tested strains of halophilic archaebacteria of the genera Halobacterium, Haloarcula, Haloferax, and Natronobacterium lysed in 1% Bacto-Peptone (Difco) containing 25% NaCl, whereas no lysis was observed with other strains belonging to archaebacteria of the genera Halococcus, Natronococcus, and Sulfolobus, methanogenic bacteria, and moderately halophilic eubacteria. Substances in Bacto-Peptone which caused lysis of halobacteria were purified and identified as taurocholic acid and glycocholic acid. High-performance liquid chromatography analyses of peptones revealed that Bacto-Peptone contained nine different bile acids, with a total content of 9.53 mg/g, whereas much lower amounts were found in Peptone Bacteriological Technical (Difco) and Oxoid Peptone. Different kinds of peptones can be used to distinguish halophilic eubacteria and archaebacteria in mixed cultures from hypersaline environments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ABRAM D, GIBBONS NE. The effect of chlorides of monovalent cations, urea, detergents, and heat on morphology and the turbidity of suspensions of red halophilic bacteria. Can J Microbiol. 1961 Oct;7:741–750. [PubMed]
  • BROWN AD, SHOREY CD. Preliminary observations on the cell envelopes of two species of Halobacterium. Biochim Biophys Acta. 1962 May 7;59:258–260. [PubMed]
  • MARCUS PI, TALALAY P. Induction and purification of alpha- and beta-hydroxysteroid dehydrogenases. J Biol Chem. 1956 Feb;218(2):661–674. [PubMed]
  • Mescher MF, Strominger JL, Watson SW. Protein and carbohydrate composition of the cell envelope of Halobacterium salinarium. J Bacteriol. 1974 Nov;120(2):945–954. [PMC free article] [PubMed]
  • Obzansky DM, Richardson KE. Quantification of urinary oxalate with oxalate oxidase from beet stems. Clin Chem. 1983 Oct;29(10):1815–1819. [PubMed]
  • Oesterhelt D, Stoeckenius W. Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. Methods Enzymol. 1974;31:667–678. [PubMed]
  • Onishi H, Kushner DJ. Mechanism of dissolution of envelopes of the extreme halophile Halobacterium cutirubrum. J Bacteriol. 1966 Feb;91(2):646–652. [PMC free article] [PubMed]
  • Pfeifer F, Betlach M. Genome organization in Halobacterium halobium: a 70 kb island of more (AT) rich DNA in the chromosome. Mol Gen Genet. 1985;198(3):449–455. [PubMed]
  • Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A. Behaviour of mixed populations of halophilic bacteria in continuous cultures. Can J Microbiol. 1980 Nov;26(11):1259–1263. [PubMed]
  • Shimada K, Sutter VL, Finegold SM. Effect of bile and desoxycholate on gram-negative anaerobic bacteria. Appl Microbiol. 1970 Nov;20(5):737–741. [PMC free article] [PubMed]
  • Sprott GD, Jarrell KF. K+, Na+, and Mg2+ content and permeability of Methanospirillum hungatei and Methanobacterium thermoautotrophicum. Can J Microbiol. 1981 Apr;27(4):444–451. [PubMed]
  • Steber J, Schleifer KH. Halococcus morrhuae: a sulfated heteropolysaccharide as the structural component of the bacterial cell wall. Arch Microbiol. 1975 Oct 27;105(2):173–177. [PubMed]
  • Vargo V, Korzeniowski M, Spaulding EH. Tryptic soy bile-kanamycin test for the indentification of Bacteroides fragilis. Appl Microbiol. 1974 Mar;27(3):480–483. [PMC free article] [PubMed]

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