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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Exp Cell Res. Author manuscript; available in PMC 2008 July 15.
Published in final edited form as:
PMCID: PMC2000818
NIHMSID: NIHMS27425

Palladin interacts with SH3 domains of SPIN90 and Src and is required for Src-induced cytoskeletal remodeling

Abstract

Palladin and SPIN90 are widely expressed proteins, which participate in modulation of actin cytoskeleton by binding to a variety of scaffold and signaling molecules. Cytoskeletal reorganization can induced by activation of signaling pathways, including the PDGF receptor and Src tyrosine kinase pathways. In this study we have analyzed the interplay between palladin, SPIN90 and Src, and characterized the role of palladin and SPIN90 in PDGF and Src-induced cytoskeletal remodeling. We show that the SH3 domains of SPIN90 and Src directly bind palladin’s poly-proline sequence and the interaction controls intracellular targeting of SPIN90. In PDGF-treated cells, palladin and SPIN90 co-localize in actin rich membrane ruffles and lamellipodia. The effect of PDGF on the cytoskeleton is at least partly mediated by the Src kinase, since PP2, a selective Src kinase family inhibitor, blocked PDGF-induced changes. Furthermore, expression of active Src kinase resulted in coordinated translocation of both palladin and SPIN90 to membrane protrusions. Knock-down of endogenous SPIN90 did not inhibit Src-induced cytoskeletal rearrangement, whereas knock-down of palladin resulted in cytoskeletal disorganization and inhibition of remodeling. Further studies showed that palladin is tyrosine phosphorylated in cells expressing active Src indicating bidirectional interplay between palladin and Src. These results may have implications in understanding the invasive and metastatic phenotype of neoplastic cells induced by Src.

Keywords: Palladin, SPIN90, Src, cytoskeleton

Introduction

Regulation of the actin microfilament cytoskeleton is important in cellular processes such as adhesion, migration and contraction. For these purposes the cytoskeleton is organized into specialized dynamic structures, such as stress fibers, focal adhesions and lamellipodia. When the cells receive new stimuli from their environment, these structures are disassembled and remodeled to meet the new requirements. The remodeling is tightly controlled by a variety of actin-binding proteins, scaffold proteins and effector proteins. An important effector is Src, a non-receptor tyrosine kinase, which contains SH3, SH2 and kinase domains. Src is activated by several mechanisms including receptor tyrosine kinase pathways[1]. Binding of platelet derived growth factor (PDGF) to its receptor leads to receptor autophosphorylation and association with Src. Activated Src translocates to the plasma membrane where it reorganizes actin cytoskeleton and induces formation of lamellipodia and membrane ruffles. This process requires a direct interaction between Src and actin-associated proteins[1, 2]. Indeed, Src kinase is shown to have several cytoskeletal substrates, including FAK, vinculin, cortactin and ezrin [3, 4]. The molecular and functional connection between Src and the structural and scaffold proteins is, however, incompletely understood. These events are of major biological and medical importance, as activation of Src occurs in several types of malignant tumors and increases tumor cell migration and invasion and hence promotes tumor dissemination[5].

Palladin is a member of the recently characterized palladin-myotilin-myopalladin protein family[6]. All family members associate with actin cytoskeleton and contain characteristic conserved IgI type domains in the C-terminal part of the protein. In striated muscle, the proteins are located in sarcomere Z-discs and control the organization of actin cytoskeleton. For instance, myotilin and myopalladin play an important role in the maintenance of sarcomere integrity and myotilin mutations lead to muscle disorders[7, 8]. The family members have also common binding partners, such as α-actinin[9]. However, the sequence, expression pattern, molecular interactions and functions clearly distinguish palladin from other family members. In contrast to myopalladin and myotilin, palladin is expressed as several alternatively spliced isoforms, termed by the number Ig-domains, in a cell-type specific manner[1012]. The most widely expressed of the isoforms, the major 3Ig isoform, has two amino-terminal proline-rich sequences, which serve as binding sites for Ena/VASP proteins, SH3 domains and profilin[1315]. On the other hand, the 4Ig isoform has an additional unique poly-proline sequence, which mediates binding LASP-1, a regulator of cell motility[12]. Palladin also interacts with the ERM-family protein ezrin and with Eps8[11, 16]. Whereas myotilin and myopalladin are mainly expressed in striated muscle, palladin has a wider pattern; it is expressed in for instance neuronal cells, fibroblasts and epithelial cells[10]. In non-muscle cells palladin is typically localized in stress fiber dense bodies, in focal adhesions and in lamellipodial regions. Previous studies have shown that depletion of palladin results in a loss of stress fibers and disruption of cell morphology thus implying that palladin is involved in the maintenance of normal actin cytoskeleton[10, 17]. Palladin is phosphorylated both on serine/threonine and tyrosine residues but the specific kinases have not been identified[10].

In the present study we set out to find new interaction partners for palladin and identified two new associated molecules, SPIN90 and Src. Palladin and SPIN90 are similarly targeted to membrane ruffles after PDGF treatment or Src activation. We show that depletion of SPIN90 does not affect Src induced actin remodeling, whereas knock-down of palladin causes disruption of stress fibers and reduction of membrane ruffle formation and thereby prevents Src-induced cytoskeletal changes. Palladin is physically associated with Src SH3 domain and active Src induces its tyrosine phosphorylation in vivo demonstrating that the interplay between the proteins occurs at several levels.

Materials and Methods

cDNA constructs and antibodies

The palladin constructs have been described before ([14, 22]. The amino acids are numbered based on the 772 residue sequence of palladin 3Ig isoform [11]. For yeast two hybrid (Y2H) experiments palladin fragments were subcloned in pLexA vector, for expression in mammalian cells in pEGFP-C or pAHP vectors and for mitochondrial targeting in pCDNA3-MOM-BiPro vector[23]. The cDNA encoding full-length SPIN90 (amino acids 1–722) and various SPIN90 fragments (ΔSH3 C-term = amino acids 280–722; SH3 = amino acids 1 – 145) were subcloned into pACTII prey vector for years two–hybrid analyses, into pGEX4T-1 (Amersham Pharmacia Biotech, Piscataway, NJ) for expression of recombinant protein in bacteria and into HA pcDNA3 (Invitrogen, Carlsbad, CA) for mammalian expression. The cDNA of the wild-type (WT) Src, constitutively activated Src containing Y529F mutation and the dominant negative kinase-dead Src K296R/Y528F in pUSEamp eukaryotic expression vector were from Upstate Biotechnology.

The SPIN90 and palladin 3Ig antibodies have been described [18,24] .A polyclonal antibody raised against the FL 3Ig mouse isoform was also used. Anti-HA mAb was from Roche and anti-phosphotyrosine antibody clone PT-66 from Sigma. Cortactin was detected with 4F11 monoclonal antibody (Upstate Biotechnology, Lake. Placid, NY).

Yeast two hybrid analysis

The bait and prey vectors (pLexA and pACT2) were cotransformed into the Saccharomyces cerevisae L40 reporter strain using a modified lithium acetate protocol. The transformants were plated onto synthetic medium lacking leucine and tryptophan. Activation of the His 3 reporter gene was assayed by restreaking the cells on plates lacking histidine, leucine, tryptophan, uracil and lysine and incubating them for 3 days at 30°C. His+ colonies were grown on synthetic medium lacking leucine and tryptophan, incubated for 3 days at 30°C and assayed for beta-galactosidase activity by a filter assay.

Cell cultures, transfections and immunofluorescence microscopy

U251mg, COS-7 and HEK293 cells were grown in Modified Eagles Medium (MEM) supplemented with 10% fetal calf serum and gentamycin. Cells were transfected using FuGENE 6 (Roche Diagnostics) transfection reagent. After 24–48 h cells grown on glass coverslips were washed and fixed with 3.5% PFA, permeabilized with 0.1% Triton X-100 and stained. HA-tagged constructs were detected with anti-HA mAb followed by rhodamine-conjugated anti-mouse IgG antibody and specimens were analyzed in Zeiss Axiophot fluorescence microscope equipped with AxioCam cooled CCD-camera.

In vitro translation, affinity/immunoprecipitation and phosphorylation assays

HA-tagged palladin 8–772 (3Ig) was in vitro translated using a rabbit reticulocyte T7 kit according to the manufacturer’s instructions (Promega). GST coupled SPIN90 FL, SH3 and C-term ( SH3) constructs were produced in E. coli strain DH5α and purified using glutathione beads (Pharmacia). In the affinity precipitation assay 5μl of 32S Met-labeled in vitro translated palladin construct was mixed with an equal amount of GST-SPIN90 beads in binding buffer (10mM Tris-HCl, pH7.4, 150mM NaCl, 1mM MgCl2, 0.1% Triton X-100). After 2h incubation at +4°C the beads were washed three times with the binding buffer and harvested in Laemmli buffer. The proteins were resolved in 10% SDS-PAGE and bound proteins were detected by autoradiography.

For co-immunoprecipitations COS-7 cells were co-transfected with EGFP-tagged SPIN90 (FL) and HA-tagged palladin 8–772 (3Ig). 36 h after transfection, the cells were washed in PBS and lysed in ELB buffer (150 mM Nacl, 50 mM HEPES pH 7.4, 5 mM EDTA, 0.5% NP-40, 1 mM dithiothreitol (DTT), 2.5 μg/ml aprotinin, 0.5 mM phenylmethylsulfonyl fluoride, 10 mM ßglycerol-phosphate and 1 μg/ml leupeptin). The lysate was centrifuged at 13 000 g for 10 min at +4°C. The cleared supernatant was incubated with 5 μl of anti-HA mAb for 1 h at +4°C prior to adding protein G-Sepharose beads for 3 h. Subsequently beads were washed four times with ELB and harvested in Laemmli buffer and subjected to Western blot analysis.

For GST-protein binding assays HEK293 cells were transfected with a HA-tagged full length SPIN90 or with a WT Src construct. The cleared lysates were allowed to bind GST or GST-Palladin construct containing glutathione-Sepharose beads. Bound proteins were resolved in a 10% SDS-PAGE gel and detected either with a SPIN90 or a Src antibody.

For phosphorylation assays HEK293 cells were co-transfected either with active Src (CA) or dominant-negative Src (DN) constructs and HA-tagged 8–772 (3Ig) palladin. As a negative control a HA-tagged N-terminal ezrin construct (amino-acids 1–309) with a Y145F mutation was used [4]. 36 h after transfection the cells were extracted in cold RIPA buffer (50 mM Tris-HCl, 1% NP-40, 150 mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1 mM orthovanadate supplemented with protease inhibitors, pH 7.4) and centrifuged at 13000 g for 10 min at +4°C. Palladin and Ezrin constructs were immunoprecipitated with anti-HA antibody. Phosphotyrosine was detected with anti-phosphotyrosine mAb PT-66 (Sigma).

siRNA experiments

To knock-down the expression of human palladin by RNA interference, two 19-base 3’-Alexa Fluor 546 labeled oligonucleotides were obtained from Qiagen. RNA sequences were: sense 5’-GCACAAAGGAUGCUGUUAU and antisense 5’-AUAACAGCAUCCUUUGUGC. As a control a non-targeting siRNA from Invitrogen was used. The SPIN90 siRNA oligonucleotides have been described previously[20]. Briefly the SPIN90 targeting si-824 psiRNA-hH1GFPzeo G2 vector was used to knock-down SPIN90 and the empty vector was used as a control.

Results

Palladin interacts with SPIN90 and Src

To identify novel interaction partners for palladin, we focused on actin cytoskeleton associated proteins which contain SH3 domain(s), a possible binding interphase with the N-terminal proline-rich regions of palldin 3Ig isoform. According to this rationale we have previously identified ArgBP2 as a palladin interacting protein [14]. SPIN90, a recently identified actin-associated protein, meets these criteria and in this study we set out to test whether it binds palladin[18]. Since the SH3 domain of SPIN90 is quite homologous (35% identity and 50% similarity) to the Src SH3 domain we also included Src in these analyses.

We first performed yeast two-hybrid analyses with palladin, SPIN90 and Src constructs. In these analyses the SH3 domain containing sequences of both SPIN90 and Src interacted with constructs containing the N-terminal part of palladin 3Ig isoform (for sequence information see Supplementary figure 1). This palladin sequence contains two proline rich stretches (residues 65–85 and 185–220) located between amino acids 8–228 (Fig. 1A). Constructs, which contained these sequences demonstrated a strong reactivity, as judged by β-galactosidase reporter color intensity, whereas constructs that lacked both poly-proline sequences did not interact with SPIN90 or Src. The second sequence was sufficient for interaction with Src but not with SPIN90.

Figure 1Figure 1
Palladin interacts with SPIN90 and Src

In order to verify the interaction with an independent in vitro method, we used an affinity precipitation assay. Palladin was in vitro translated using reticulocyte lysates and allowed to bind to SPIN90 and Src sequences fused to GST and coupled on glutathione-Sepharose beads. Bound proteins were eluted, run in SDS-PAGE and detected by autoradiography. The results show that the SH3 domains of SPIN90 and Src are required and sufficient to mediate the interaction (Fig. 1B). The role of palladin’s proline-rich sequences in the interactions was studied in more detail with an affinity precipitation assay (Fig. 1C). In this assay both halves of the palladin’s N-terminus, containing either the first or the second poly-proline stretch, could mediate binding to SPIN90 and Src. While SPIN90 bound roughly equally to either half, Src seemed to have a preference to palladin’s second proline-rich stretch but the binding constants were not experimentally quantitated.

To study whether palladin binds to SPIN90 also in vivo, an immunoprecipitation assay was used. COS-7 cells were transiently co-transfected with HA-tagged palladin and GFP-tagged SPIN90. After 24 h the cells were lysed and HA-palladin was precipitated with an anti-HA antibody and protein G-Sepharose beads and the precipitates were immunoblotted for HA and GFP. Both HA-palladin and GFP-SPIN90 could be detected in the precipitate indicating that a complex could be formed in vivo (Fig. 1D).

Phosphorylation of palladin in Src CA transfected cells

To further investigate the interplay between palladin, SPIN90 and Src, we tested, whether palladin can serve as a substrate for Src, as previously shown for SPIN90 (also known as DIP) [32]. An HA-tagged construct of palladin (3Ig) was co-expressed with constitutively active (CA) Src in HEK293 cells and the transfected proteins were subsequently immunoprecipitated with an anti-HA antibody. Blotting of the precipitates with a phospho-tyrosine antibody demonstrated a clear reactivity in palladin precipitate (Fig. 2). When palladin was co-expressed with a dominant negative (DN) Src construct, tyrosine phosphorylation of palladin was not detected (Fig. 2).

Figure 2
Palladin is tyrosine phosphorylated in cells expressing active Src kinase

Role of the interaction in subcellular targeting of SPIN90

In order to study whether the interaction with palladin regulates the subcellular distribution of SPIN90 we used two different in vivo targeting assays. We have previously shown that overexpression of palladin in COS-7 cells results in reorganization of the cytoskeleton and formation of thick actin bundles[12, 22]. We analyzed the behavior of HA-tagged SPIN90 (SPIN90-HA) and similarly tagged SH3 domain of PIN90 (SPIN90-SH3-HA) constructs expressed alone or together with GFP-palladin 8–772. When transfected alone, SPIN90 and SPIN90-SH3 constructs were distributed diffusely in the cytoplasm (Fig. 3A and 3D). On the other hand, cotranfrection with palladin resulted in targeting of both SPIN90 constructs to the newly formed actin bundles thus indicating that palladin can target SPIN90 (Fig. 3B–C and 3E–F).

Figure 3Figure 3
Subcellular targeting of SPIN90 by palladin

To study, whether the targeting requires direct interaction between the proteins, we used a mitochondrial targeting assay. Palladin 8–228 construct containing the poly-proline sequences was fused with a Mitochondria Outer Membrane (MOM) targeting sequence. This construct was co-transfected with SPIN90-SH3-HA construct to CHO cells. Palladin-8-228-MOM was able to relocalize the SPIN90-SH3 from its normal diffuselocation to the mitochondria (Fig. 3G–H). On the other hand co-expression of palladin 222-387-MOM with SPIN90-SH3 did not result in relocalization of the SPIN90 construct (Fig. 3J–K).

The effect of PDGF treatment and activation of the Src-kinase on palladin and SPIN90 localization

To evaluate, how signaling pathways signaling modulates palladin and SPIN90, we transfected SPIN90 in U251 glioma cells, which express high amounts of endogenous palladin. Under basal conditions co-localization of the two proteins was limited to the lamellipodial extensions of motile cells (not shown). In non-migrating cells palladin localized to the stress fiber dense regions as previously described, while SPIN90 localized rather uniformly through out the cytoplasm (Fig. 4). Both SPIN90 and palladin have been shown to relocate to membrane ruffles after PDGF treatment. In these structures SPIN90 interacts with the Arp2/3 complex and palladin with Eps8 [16, 20]. As expected, stimulation of serum-starved U251 cells with PDGF for 10 minutes resulted in formation of numerous membrane ruffles and wave-like actin rich structures. Both transfected (not shown) and endogenous palladin rapidly re-located to these newly formed structures where it co-localized with SPIN90 and with cortactin (Fig. 4).

Figure 4
Localization of SPIN90 and palladin in PDGF treated U251 cells

Since the effect of PDGF on actin cytoskeleton has been shown to be, at least partly, mediated by the Src family tyrosine kinases we next studied whether inhibition of kinase activity with Src-selective inhibitor PP2 would have an effect on the re-localization of SPIN90 and palladin in PDGF stimulated cells. As shown in Fig. 4, PP2 completely abolished the formation of the membrane ruffles and thus the re-distribution of SPIN90 and palladin. Similarly, cortactin remained diffusely distributed in the cytoplasm in PP2 treated cells.

To further study the role of Src activity in targeting of SPIN90 and palladin, we expressed either active (CA) or dominant negative (DN) Src constructs in U251 cells. Introduction of active Src in U251 cells resulted in a rapid re-organization of the actin cytoskeleton and drastic changes in cell morphology. Both SPIN90 and palladin re-localized to the Src induced actin rich membrane protrusions. In cells expressing a dominant negative (DN) Src construct both palladin and SPIN90 remained in their normal position (Fig. 5).

Figure 5
SPIN90 and palladin co-localize in cells expressing active Src

Depletion of palladin leads to cytoskeletal disruption and loss of stress fibers

In order to study the role of palladin in cytoskeletal organization, we designed palladin specific siRNA oligonucleotides and transfected them to U251 cells. As seen in Figure 6, siRNA treatment resulted in a marked down-regulation of palladin as verified by Western Blot analysis and immunofluorescence staining of transfected cells. The remaining weak palladin staining was more diffuse than in control cells, in which palladin was associated with stress fibers. F-actin staining of the treated cells with fluorescent phalloidin demonstrated disruption of stress fibers, disorganization of the actin cytoskeleton and defective cellular spreading. These results are in line with previous studies, in which palladin was down regulated either genetically with an anti-sense cDNA construct[10, 17].

Figure 6
Depletion of palladin results in disruption of actin cytoskeleton and loss of stress fibers

Palladin is required for Src induced actin remodeling

Previous studies have indicated a role for both SPIN90 and palladin in PDGF induced membrane ruffle formation [16, 20]. We wanted to examine whether this is true also for Src-kinase induced actin remodeling. U251 cells were co-transfected with active Src construct and either with SPIN90 specific siRNA construct (siRNA 824-GFP), empty control vector (siVector-GFP) or with a palladin specific siRNA oligonucleotide. Although SPIN90 siRNA efficiently down-regulated SPIN90 expression, we did not detect cytoskeletal differences between cells expressing control siRNA and the specific SPIN90 siRNA together with Src CA. In the presence or absence of SPIN90, Src induced remodeling of actin cytoskeleton and formation of numerous membrane extensions. These newly formed structures contained actin, palladin and also Src (Fig. 7). However, when palladin was knocked-down with a specific siRNA, the effect of Src Ca on the actin cytoskeleton were inhibited. Fewer membrane protrusions were formed in cells co-transfected with palladin siRNA and Src CA than in cells expressing only the Src CA construct. Also, Src kinase, SPIN90 and cortactin were distributed more diffusely in palladin knock-down cells. Taken together, while both palladin and SPIN90 are needed for PDGF induced membrane ruffling, palladin seems to play a more prominent role in Src induced remodeling of actin cytoskeleton.

Figure 7Figure 7Figure 7
The role of palladin and SPIN90 in Src induced actin cytoskeleton remodeling

Discussion

In this work we describe a novel interaction between palladin, SPIN90 and Src. SPIN90 (also known as DIP, mDia interacting protein) contains an SH3 domain, three proline-rich regions, a serine/threonine-rich region and a long C-terminus and shows high sequence similarity to WISH [18]. SPIN90 interacts with several actin-regulating proteins including Nck, N-WASP, βPIX, the Arp2/3 complex, PSD-95 and mDia [19, 20,39,32]. It plays a role in myofibril and sarcomere assembly and depletion of SPIN90 in cardiomyocytes results in sarcomere disruption. Furthermore, it participates in the regulation of filopodia and dendritic spines in neuronal cells [34] and in actin-based motility by promoting the Arp2/3 activity. Finally, SPIN90 can be modulated by ERK1, a kinase activated by cell adhesion or PDGF[21].

Previous reports on palladin and SPIN90 suggest that both proteins act as potent cytoskeletal scaffolds bringing together proteins with different functional modalities into higher-order molecular complexes, whose localization and composition can vary in different cell types and situations. Their role as scaffolds is supported by several observations: (1) the proteins do not have intrinsic enzymatic activity, (2) they contain several domain structures that serve as interaction platforms for actin-associated proteins, (3) the proteins serve as substrates for cytoskeleton modulating kinases, (4) they localize at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles, and (5) their role in actin remodeling has been demonstrated in knock-down experiments[16, 20]. The present study establishes a molecular link between palladin and SPIN90 but also demonstrates differences in their ability to regulate actin cytoskeleton.

The interaction is mediated by the poly-proline sequences of palladin and the SH3 domain of SPIN90. Palladin differs from the two other protein family members, myotilin and myopalladin, in that its amino-terminus contains proline-rich stretches, which serve as ligands for SH3, EVH and WW domains and also for profilin[26]. One of these proline sequences is conserved between myopalladin and the palladin isoform, 4Ig, and this particular sequence has been shown to mediate direct binding between nebulin and myopalladin[27] and the SH3 domain of LASP-1 and palladin 4Ig[12]. The LASP-1 binding motif is not contained in the major shorter palladin isoform, 3Ig, whose N-terminal poly-prolines bind to the first SH3 domain of ArgBP2 [14], to the EVH domain of VASP [13] and to profilin II[28]. The amino-terminus also binds another SH3 domain protein, Eps8, but this interaction is not mediated by the SH3 domain[16]. While the interactions between SH3 domains and poly-proline sequences are specific, they are usually of low affinity, suggesting that they can be rather short-lived and dynamically regulated by both intra- and extracellular cues. This could also be the case between palladin and SPIN90 because in non-muscle cells the proteins co-localize and thus probably interact in actively remodeled actin rich structures such as lamellipodia.

Our results further demonstrate a bidirectional link between palladin and Src. Src activation results in phosphorylation of palladin in cells and, on the other hand, palladin is involved in subcellular targeting of Src. Src family kinases regulate three main cellular functions that ultimately control the behavior of transformed cells: adhesion, invasion and motility, all of which require active remodeling of the actin cytoskeleton[1, 29]. Src has been previously shown to have several focal adhesion and cytoskeletal substrates, including FAK, vinculin, paxillin, cortactin and ezrin[2, 4]. Activation of Src induces rapid reorganization of the actin cytoskeleton and formation of structures such as lamellipodia and podosomes. Src can be activated by several mechanisms including ligation of receptor tyrosine kinases such as PDGFR and EGFR. When Src is activated by PDGF treatment it rapidly redistributes from central perinuclear location to the plasma membrane. This process requires intact stress fibers and is mediated by the SH3 domain of Src[30, 31,35]. Our results show that knock-down of palladin results in disruption of stress fibers and subsequent inhibition of the relocalization of active Src and SPIN90. Since the formation of membrane ruffles and lamellipodia requires Src activity beneath the plasma membrane, targeting of Src by palladin may be a mechanism to control Src-dependent events.

Similarly to palladin, SPIN90 links directly to Src signaling. Meng et al. showed that SPIN-90 (DIP) is a Src substrate and is involved in targeting effector proteins such as Vav2 and p190RhoGAP [32]. SPIN90 binds to both p190RhoGAP and Vav2 and targets them to the plasma membrane, where all the three components of this complex are phosphorylated by Src. Inhibition of SPIN90 by dominant-negative mutants or siRNA prevented EGF induced actin organization, redistribution of p190RhoGAP and Vav2 and cell movement. SPIN90 inactivates Rho and activates Rac via p190RhoGAP and Vav2, respectively, in a Src dependent manner. Inactivation of Rho leads to loss of stress fibers and activation of Rac to formation of lamellipodia. Our results show that the knock-down of SPIN90 does not affect the targeting of palladin and Src, in line with the results of Meng et al, who showed that SPIN90 (DIP) did not target Src to the plasma membrane [32]. Together these results offer a basis for a model where Src and SPIN90 translocate after stimulation to the membrane via a palladin/stress fiber dependent manner. At the membrane active Src phosphorylates p190RhoGAP and Vav2 via SPIN90 leading to loss of the stress fibers and subsequently formation of lamellipodia and cell movement. Since palladin and SPIN90 have been shown to have several interaction partners localizing to membrane ruffles and lamellipodia it is possible that the interactions can also vary during different stages of lamellipodia organization. In a recent study by Eisenmann et al SPIN90 induced non-apoptotic membrane blebbing, which is known to be associated with amoeboid cell movement and thus cancer-cell migration [25]. This novel finding offers additional support for the assumption that SPIN90 plays an important role in organizing the membrane associated cytoskeleton and cellular movement. Furthermore, fibroblasts derived from palladin negative murine embryos have been to shown to have a greatly diminished motility [17].

Previously, palladin has been shown to be phosphorylated both on tyrosine and serine/threonine residues but kinases involved in this have not been identified[10]. Interestingly, PDGF treatment of A7r5 smooth muscle cells leads to reduced migration of palladin in SDS-PAGE suggesting post-translational modification, possibly phosphorylation of the protein, but this preliminary finding needs still to be experimentally verified[16]. We extend these findings by showing that palladin becomes tyrosine phosphorylated in cells expressing active Src. It remains to be shown, whether Src directly phosphorylates palladin, or whether a kinase downstream of Src would be the actual effector. One of such potential kinases could be FAK.

The role of tyrosine phosphorylation of cytoskeletal proteins is still mostly unclear. One of the best characterized cytoskeletal substrates of Src is cortactin. Cortactin phosphorylation serves a variety of functions, one of which is to provide binding sites for specific signaling proteins with SH2 domains, e.g. Src family kinases and Nck[33]. Tyrosine phosphorylation may also induce other types of novel interactions as shown between ezrin and a kelch-repeat protein family member, KTBDB2[4]. The effects of Src can also be mediated indirectly by other effector proteins, for example by the MAPK family members such as ERK. Src has been shown to directly activate ERK, which is of particular interest since SPIN90 is a substrate of ERK[21]. Phosphorylation of SPIN90 by ERK1 promotes the interaction between SPIN90-βPIX-WASP complex and the adaptor protein Nck[21]. These findings offer some clues for the consequences of palladin phosphorylation, but further experiments are needed to elucidate its specific function.

In conclusion our results show that palladin interacts directly with both SPIN90 and Src and that palladin plays a role in maintaining stress fiber integrity and targeting of SPIN90 and Src to sites where active actin remodeling takes place. Thus palladin can act as a true protein scaffold bringing together effector proteins and their cyroskeletal targets.

Supplementary Material

01

Supplementary figure 1. A schematic illustration of the palladin’s poly-proline sequences

Above is the amino-terminal (amino-acids 1–228) protein sequence of the major palladin 3Ig isoform (protein accession KIAA0992). Below are the sequences of the three GST-constructs used in the pull-down assays. Proline residues are in capital and the two especially proline-rich sequences located between amino-acids 65–85 and 185–220 are in italic.

Acknowledgments

We thank Helena Ahola for expert technical assistance. This work was supported by grants from the Academy of Finland, Sigrid Juselius Foundation, and Finnish Heart Association, by NIH grants GM61743 and NS43253 to Carol Otey and grants from the National Research Laboratory (The Ministry of Science & Technology) and the Brain Korea 21 Program to Woo Keun Song.

Footnotes

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