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Acute hepatic failure secondary to paracetamol poisoning is associated with high mortality. C‐jun (NH2) terminal kinase (JNK) is a member of the mitogen‐activated protein kinase family and is a key intracellular signalling molecule involved in controlling the fate of cells.
To examine the role of JNK in paracetamol‐induced acute liver failure (ALF).
A previously developed mouse model of paracetamol poisoning was used to examine the role of JNK in paracetamol‐induced ALF.
Paracetamol‐induced hepatic JNK activation both in human and murine paracetamol hepatotoxicity and in our murine model preceded the onset of hepatocyte death. JNK inhibition in vivo (using two JNK inhibitors with different mechanisms of action) markedly reduced mortality in murine paracetamol hepatotoxicity, with a significant reduction in hepatic necrosis and apoptosis. In addition, delayed administration of the JNK inhibitor was more effective than N‐acetylcysteine after paracetamol poisoning in mice. JNK inhibition was not protective in acute carbon tetrachloride‐mediated or anti‐Fas antibody‐mediated hepatic injury, suggesting specificity for the role of JNK in paracetamol hepatotoxicity. Furthermore, disruption of the JNK1 or JNK2 genes did not protect against paracetamol‐induced hepatic damage. Pharmacological JNK inhibition had no effect on paracetamol metabolism, but markedly inhibited hepatic tumour necrosis foctor α (TNF α) production after paracetamol poisoning.
These data demonstrated a central role for JNK in the pathogenesis of paracetamol‐induced liver failure, thereby identifying JNK as an important therapeutic target in the treatment of paracetamol hepatotoxicity.
Paracetamol (acetaminophen) poisoning is the most common cause of acute liver failure (ALF) in the UK and US.1,2 Deaths from paracetamol‐induced ALF still occur despite the availability of an effective antidote (N‐acetylcysteine (NAC) or methionine) and changes in the sale and packaging of paracetamol in the UK. The only effective treatment for severe cases of paracetamol hepatotoxicity is orthotopic liver transplantation. However, transplantation has disadvantages including limited donors, the commitment of often young recipients to lifelong immunosuppression and psychiatric or medical contraindications in approximately 50% of patients.3 Effective alternative treatments for paracetamol‐induced ALF are urgently required.
The metabolic activation of paracetamol to N‐acetyl‐p‐quinoneimine (NAPQI) and subsequent conjugation with glutathione is well characterised.4 However, the pathways linking paracetamol metabolism with hepatic necrosis remain elusive. Recent investigations have expanded our understanding of the complex interplay between the pathways of toxin metabolism, intracellular signalling and the host inflammatory response.5,6 Inflammatory cells play a permissive role in hepatic necrosis induced by paracetamol and early‐response cytokines and chemokines modulate both the injury and repair processes.7,8 The mitogen‐activated protein kinases are a family of serine/threonine kinases that are evolutionarily conserved signal‐transducing enzymes unique to eukaryotes.9,10 The c‐jun (NH2) terminal kinases (JNKs) are one subgroup of the mitogen‐activated protein kinases.11,12 The JNK signal transduction pathway is stimulated by a diverse range of extracellular and intracellular stimuli including reactive oxygen species (ROS), ultraviolet radiation and pro‐inflammatory cytokines. JNK controls many basic mammalian physiological processes and phosphorylates specific subunits (c‐Jun, JunB, JunD and activating transcription factor 2) of the activating protein 1 (AP‐1) transcription factor,13,14,15 activating genes that regulate diverse cellular functions including proliferation,16 survival17,18 and death.19,20 Recent in vitro data21,22,23,24 and in vivo studies25,26,27 suggest a role for JNK in mediating hepatic injury. Using a previously developed mouse model of paracetamol poisoning,8,28 we demonstrate a central role for JNK in the pathogenesis of paracetamol‐induced ALF, thereby identifying JNK as a potential therapeutic target.
Archival human liver samples were obtained from the Department of Pathology, University of Edinburgh, Edinburgh, UK. All animal procedures were undertaken using C57/BL6 mice, with approved licence from the Animal Scientific Procedures Division of the Home Office (London, UK) using mice aged 8–10 weeks. Generation of JNK1−/− and JNK2−/− mice by gene‐targeting technology (on a C57/BL6 background) has been described previously.29 As control, age‐ and sex‐matched wild‐type (WT) littermates were used. After an overnight fast, mice were injected intraperitoneally (IP) with 350 mg/kg of paracetamol dissolved in sterile phosphate‐buffered saline (PBS) and warmed to 42°C as described previously,28 with carbon tetrachloride (CCl4) at a ratio of 1:3 with olive oil at a dose of 1 μl/g body weight as described previously30 or with anti‐Fas antibody (0.5 μg/g body weight; BD Pharmingen, UK). Two JNK inhibitors were used in this study: SP600125 (anthra[1,9‐cd]pyrazol‐6(2H)‐one) purchased from Calbiochem (San Diego, California, USA) and d‐JNKI1 (c‐Jun N‐terminal kinase peptide inhibitor 1, d‐stereoisomer) inhibitor purchased from Axxora (Nottingham, UK). The vehicle control for SP600125 was 40% polyethylene glycol (PEG; Sigma, UK) in PBS. The control for d‐JNKI1 was d‐TAT control peptide (Axxora) in PBS. In experiments utilising delayed JNK inhibition, pharmacological inhibitors or appropriate controls were administered IP 5 h after paracetamol injection.
After IP injection, groups of mice were scored as follows by a blinded observer for signs of systemic illness: clinically well (0 points), presence of piloerection (1 point), with additional hunched posture (2 points) and lack of spontaneous movement (3 points) 24 h after injection. Serum was stored at −80°C until use for determination of alanine aminotransferase (ALT) levels by an automated enzyme assay (Olympus 20700 analyser (Olympus UK Ltd, Southall, UK)). Two lobes from each liver were fixed in 4% paraformalydehyde before routine histological processing. Histological grading of hepatic necrosis was performed by two blinded observers using H&E‐stained sections as follows: <30% of the total area necrotic (1 point); 30–60% of the total area necrotic (2 points); >60% of the total area necrotic (3 points). Ten random fields were counted independently by two blinded observers to determine the number of apoptotic cells. Whole‐liver homogenates were prepared and the Cell Death Detection ELISA (Roche, Welwyn Garden City, UK; quantitative detection of histone‐associated DNA fragments in mononucleosomes and oligonucleosomes) was used as per the manufacturer's instructions.
Paraffin‐wax‐embedded sections were deparaffinised and subjected to microwave antigen retrieval in citrate buffer. The primary antibodies were rabbit polyclonal anti‐phospho‐JNK (Biosource, Nivelles, Belgium) and antiparacetamol–protein adduct antibodies (HyCult Biotechnology, Uden, Netherlands). Species‐appropriate isotype control antibodies were also used for each experiment. Western blot analysis was undertaken by using the following primary antibodies: rabbit polyclonal anti‐phospho‐JNK 1&2 [pTpY183/185] (Biosource), rabbit polyclonal anti‐phospho‐p38 antibody [pTpY180/182] (Biosource, Belgium) and rabbit polyclonal anti‐β‐actin antibody (Sigma, Gillingham, UK). JNK activity was measured as described previously.31 Liver was homogenised in PBS with a protease inhibitor cocktail (Roche), and levels of immunoreactive tumour necrosis factor α (TNFα) and interferon γ (IFNγ) were measured in the supernatants by ELISA according to manufacturer's instructions (R&D systems, Abingdon, UK).
Results are presented as means (SEM). Significance of the differences between means was assessed using one‐way analysis of variance or two‐tailed Student's t test. Values of p<0.05 were considered significant. Unless stated otherwise, studies were performed on 3–6 independent occasions using 6–8 mice per group.
To determine whether JNK activity is increased during paracetamol‐induced liver injury, we examined phospho‐JNK expression. In a normal human liver, constitutive JNK phosphorylation occurs in areas surrounding the central veins (fig 1A1A).). Cytoplasmic and dense nuclear staining was observed within hepatocytes. After paracetamol‐induced ALF, there was a marked increase in hepatocyte cytoplasmic and nuclear phospho‐JNK staining compared with the control normal liver (fig 1B1B;; n=8 cases in each group). We also examined JNK activation in a murine model of paracetamol‐induced hepatic injury. Phospho‐JNK expression was negligible in the control mouse liver (fig 1C1C).). Similar to human cases, paracetamol‐injured mouse liver demonstrated marked upregulation of phospho‐JNK in the hepatocyte nuclei and cytoplasm in the areas surrounding the central veins (fig 1D1D).). The time course of JNK activation after paracetamol‐induced injury was examined: hepatic JNK activity drastically increased after paracetamol administration, with peak activity 2 h after injection (fig 1E1E and inset). JNK activity then returned towards baseline levels by 24 h. Comparison with the time course of biochemical liver injury (determined by serum ALT) showed peak ALT 5 h after paracetamol injection (fig 1F1F).
To assess the pathogenic significance of the observed increase in JNK activity, we used two pharmacological inhibitors of JNK that differ in their mechanisms of action. SP600125 is a small‐molecule reversible ATP‐competitive inhibitor,32,33 whereas d‐JNKI1 is a peptide inhibitor that inhibits the interaction of JNK with its substrates.34 Immunohistochemistry for hepatic phospho‐JNK demonstrated potent inhibition of JNK phosphorylation by SP600125 2 h after paracetamol treatment compared with control (fig 2A,B2A,B)) and was confirmed by radioactive kinase assay (fig 2C2C)) and western blotting for phospho‐JNK (fig 2D2D).). Importantly, pretreatment with SP600125 did not inhibit phosphorylation of the other major stress‐activated protein kinase in the liver, p‐38 (fig 2D2D).). To determine the potential clinical significance of JNK activation after paracetamol poisoning, the JNK inhibitors (or control) were injected 1 h before paracetamol administration, and mortality was assessed over 72 h. Inhibition of JNK activity with either SP600125 or D‐JNKI1 drastically reduced mortality compared with the vehicle control (fig 2E2E).). In addition, sickness scores were assessed at 24 h, and the SP600125 and d‐JNKI1 inhibitor groups showed significantly lower sickness scores than the vehicle control group (fig 2F2F).
To further evaluate the striking survival benefit of JNK inhibition after paracetamol administration, liver injury was assessed after JNK inhibition. SP600125 or d‐JNKI1 dramatically reduced histological liver injury and hepatic necrosis scores compared with vehicle control (fig 3A,B3A,B).). Furthermore, hepatocyte necrosis, as assessed by serum ALT release, was also reduced in the JNK inhibitor groups compared with vehicle control (fig 3C3C).). Hepatocyte apoptosis may occur during paracetamol toxicity in the human and murine liver.35 Apoptotic cells were clearly visible in the control group 5 h after paracetamol administration; however, apoptotic cells were less abundant in the JNK inhibitor groups at this time point (fig 3D3D).). Counting of morphologically apoptotic cells confirmed this finding, with a significant reduction in the number of apoptotic hepatocytes in the inhibitor groups compared with the control, 5 h after paracetamol administration (fig 3E3E).). Further assessment of apoptosis by cell death ELISA using whole‐liver homogenates also demonstrated a significant reduction in apoptosis in the JNK inhibitor groups 1, 2 and 5 h after paracetamol treatment compared with the control (fig 3F3F,, p<0.01).
An important clinical problem in the management of paracetamol poisoning is the group of patients who present 15 h after the overdose when the antidote, NAC, is much less effective. We have previously used NAC in a mouse model of paracetamol toxicity36 and demonstrated that beyond 5 h NAC is no longer effective in preventing liver injury. Therefore, we investigated whether delayed administration of the JNK inhibitor is more effective than NAC when given after a paracetamol overdose. Histological liver injury was less in the NAC and SP600125 groups (fig 4A4A).). Furthermore, SP600125 was significantly more effective than NAC in limiting liver injury after paracetamol poisoning when given 5 h after an overdose, as assessed by hepatic necrosis scoring (fig 4B4B)) and serum ALT release (fig 4C4C).). However, JNK inhibition was no longer effective 8 and 24 h after paracetamol poisoning.
To determine whether JNK inhibition is protective in other forms of acute liver injury, we examined JNK inhibition in acute CCl4‐ and anti‐Fas antibody‐induced hepatic damage. Similar to paracetamol, CCl4 is a hepatotoxin metabolised in the liver by the cytochrome system, leading to the release of toxic free radicals and oxidant‐mediated hepatic injury. Also, CCl4 injection increases JNK activity in the whole liver in vivo.37 To assess the effect of JNK inhibition in CCl4‐mediated liver injury, mice received CCl4 (at a ratio of 1:3 with olive oil at a dose of 1 μl/g body weight) 1 h after treatment with control, SP600125 or d‐JNKI1. Similar histological liver injury was observed in all groups (fig 5A5A),), and there was no significant difference in hepatic necrosis scores (fig 5B5B)) or biochemical liver injury at 24 h (fig 5C5C).). Further experiments were also undertaken using CCl4 at a ratio of 1:3 with olive oil at a dose of 0.5 μl/g body weight, with no protection provided by JNK inhibition.
Previous studies have suggested that the Fas death pathway may play a role in the pathogenesis of paracetamol‐induced liver injury.38,39,40 Therefore, we specifically examined the role of JNK inhibition in the anti‐Fas antibody model of hepatic injury. Administration of anti‐Fas (Jo‐2) antibody directly ligates and activates the CD95 death receptor pathway, resulting in massive hepatocyte apoptosis within hours.41,42 Mice received anti‐Fas antibody 1 h after treatment with control, SP600125 or d‐JNKI1. Similar histological liver injury was observed in all groups (fig 5D5D),), and there was no significant difference in hepatic necrosis scores (fig 5E5E)) or biochemical liver injury at 6 h (fig 5F5F).). Further experiments were also undertaken using anti‐Fas antibody at a dose of 0.25 μg/g body weight, with no protection provided by JNK inhibition (data not shown).
The JNK protein kinases are encoded by three genes.11 The liver expresses two of the three known JNK genes—JNK1 and JNK2. To discuss in further detail the role of JNK1 and JNK2 in paracetamol‐induced hepatic failure, we studied JNK1−/− and JNK2−/− mice (JNK1+2 double‐null mice are embryonic lethal). Liver histology showed no protection in the JNK1−/− and JNK2−/− mice compared with WT mice (fig 6A6A),), and no significant difference in hepatic necrosis scores (fig 6B6B)) or biochemical liver injury between groups (fig 6C6C).
Ingestion of paracetamol results in metabolism to NAPQI, which is effectively detoxified by conjugation with glutathione. However, in paracetamol poisoning, cytosolic and mitochondrial glutathione becomes depleted, allowing covalent binding of NAPQI to hepatocellular proteins (paracetamol–protein adducts).4 Although the exact mechanisms mediating paracetamol hepatotoxicity remain elusive, recent data have highlighted the important regulatory role of the immune system and cytokine networks in determining outcomes after paracetamol‐induced liver injury.8,28,36,39,43 Therefore, we examined whether JNK inhibition interferes with the metabolism of paracetamol by measuring paracetamol–protein adducts.44 No paracetamol–protein adducts were seen in normal mouse liver (fig 7A7A).). However, the formation of paracetamol–protein adducts (visible in hepatocytes around the central veins) was similar in all groups 5 h after paracetamol administration (vehicle control, SP600125 and d‐JNKI1 groups; fig 7B–D).
We also explored in vivo which inflammatory cytokines may be modulated by JNK inhibition in our model of paracetamol‐induced liver injury. Previous studies have implicated interferon γ (IFNγ) in the pathogenesis of paracetamol‐induced liver injury.7,39 Hepatic IFNγ levels were increased in all groups 2 h after paracetamol administration compared with the control, and remained increased throughout 24 h. No significant difference in hepatic IFNγ levels was observed between the treatment groups at any of the time points studied (fig 7E7E).
TNFα is an inflammatory cytokine that may play a significant pathogenic role in paracetamol hepatotoxicity,28,45,46 although conflicting data have been reported. In vitro studies show that TNFα induces JNK activation in hepatocytes.47 In addition to the role of TNFα in apoptotic hepatocyte death, perhaps more relevant to paracetamol‐induced liver injury, the JNK/AP‐1 signalling pathway can also mediate TNFα‐induced necrotic hepatocyte death.48 JNK activation can also induce TNFα expression via AP‐1.49,50 Therefore, we examined whether the protective effect of JNK inhibition in paracetamol hepatotoxicity is mediated by modulation of TNFα expression. Hepatic TNFα levels were significantly increased in the vehicle control group 2 and 5 h after paracetamol administration compared with baseline (0 h). However, there was significantly less hepatic TNFα production in the JNK inhibitor groups 2 and 5 h after paracetamol administration compared with the vehicle control (fig 7F7F).
We have shown a central role for JNK in the pathogenesis of paracetamol‐induced ALF, thereby identifying JNK as a potential therapeutic target in the treatment of paracetamol hepatotoxicity. Our data indicated that: (1) paracetamol‐induced liver injury results in hepatic JNK activation in human and murine tissue, which precedes the onset of hepatocyte death; (2) pharmacological inhibition of JNK in vivo markedly improved survival in a mouse model of paracetamol‐induced ALF and decreased both hepatic necrosis and apoptosis. The protective effect of JNK inhibition was specific for paracetamol hepatotoxicity as the inhibitors were not protective in acute CCl4‐mediated or anti‐Fas antibody‐mediated hepatic injury; (3) delayed administration of JNK inhibitor is more effective than NAC in limiting liver injury in mice; (4) disruption of either the JNK1 or JNK2 genes was not protective; and (5) inhibition of the JNK had no effect on paracetamol metabolism, but inhibited hepatic TNFα production after paracetamol‐induced ALF.
Paracetamol poisoning is the most common cause of ALF in the UK and USA, causing significant morbidity and mortality in predominantly young people. Many patients present outside the therapeutic window for the commonly used antidote, NAC. Our data demonstrated that delayed administration of the JNK inhibitor is more effective than NAC after paracetamol poisoning in mice. Although NAC can be administered up to 15 h after a paracetamol overdose in clinical practice, we found limited efficacy 5 h after paracetamol administration in the mouse model. This is in keeping with our previously published data,36 and relates to the truncated time course of liver injury in mice compared with humans. Metabolic activation of paracetamol and the histological liver injury produced in mice is similar to that observed in humans; however, the variability in terms of the clinical context in which the paracetamol overdose is taken and the genetic background of humans limits direct extrapolations of our results to the treatment of patients. Despite these limitations, our data indicate that further studies of JNK activation in cases of paracetamol poisoning in humans may lead to the development of new treatments and that pharmacological inhibition of JNK may be of particular clinical use in patients with delayed presentation after paracetamol overdose.
Our data show prolonged hepatic activation of JNK after paracetamol overdose in both the human and murine liver. Interestingly, in the normal human liver phospho‐JNK staining was observed in hepatocytes surrounding the central veins, the area in which paracetamol is preferentially activated by the hepatic cytochrome P450 system. Hepatic JNK activation may be a consequence of the oxidative stress produced during paracetamol metabolism. ROS are widely recognised to induce increased and/or prolonged JNK activation, possibly because of inactivation of cellular phosphatases.25 Although ROS alone can activate JNK, hepatic TNFα expression is increased in paracetamol‐induced liver injury.51,52,53 TNFα is a potent inducer of both JNK and ROS. Therefore, in the hepatic cytokine milieu induced by paracetamol, a positive amplification loop may exist whereby paracetamol‐induced ROS leads to JNK activation and TNFα expression via AP‐1,49,50 resulting in further prolonged massive JNK activation in the liver via TNF receptor signalling.
To investigate the potential pathogenic role of JNK activation in the development of paracetamol‐induced hepatic necrosis, we used two different JNK inhibitors with different mechanisms of action (SP600125 and d‐JNKI1). Both inhibitors have been widely studied both in vivo and in vitro. Although the specificity of pharmacological inhibitors can be questioned, we found no reduction in the hepatic activation of another stress‐activated protein kinase, p38, after administration of the JNK inhibitor and paracetamol. Furthermore, despite different mechanisms of action, both JNK inhibitors conferred significant survival benefit and profound protection against paracetamol‐induced hepatic necrosis.
Several studies have shown the mechanistic importance of the hepatic cytokine network in paracetamol‐induced liver injury. To investigate the mechanisms underlying the protective effect of JNK inhibition in paracetamol hepatotoxicity, we measured the hepatic expression of IFNγ and TNFα. Both these cytokines are induced in the liver after paracetamol poisoning.7,39,51,52,53 Furthermore, IFNγ knockout mice are resistant to paracetamol‐induced liver injury.7 However, in our model, JNK inhibition had no significant effect on hepatic IFNγ. By contrast, JNK inhibition significantly reduced hepatic TNFα expression. We and others have shown increased TNFα expression in the peripheral circulation and liver after paracetamol poisoning.28,53 In the studies reporting a protective effect of TNFα inhibition in paracetamol‐induced liver injury, the degree of protection was similar to that observed with JNK inhibition in our study.45,51,52 However, the pathogenic role of TNFα in paracetamol‐induced hepatic necrosis remains controversial. Studies using blocking antibodies and inhibitors of TNFα have produced conflicting results, with both protection and no effect being reported.51,52,53 Alternatively, some have shown attenuated liver injury in mice lacking TNF receptor 1 (TNFR1) expression, while others, using a different strain of mice, have shown increased liver injury in TNFR1 knockout mice.45,54 Our data suggest that JNK inhibition may limit liver injury via reduced TNFα expression, although in view of the reduced initial liver injury with JNK inhibition it is difficult to clarify a potential cause or an effect of the reduced TNFα expression. In a recent study, Kaplowitz et al reported that JNK inhibition limited paracetamol‐induced liver injury, possibly by interfering with translocation of members of the Bcl2 family into the mitochondrial membrane.55 This study reported that JNK inhibition protects TNFR1 knockout mice from paracetamol‐induced hepatic injury. However, TNFα expression levels were not measured in this study. These data do not invalidate our postulated mechanism as TNFα can also induce cell death via TNFR2 signalling.56,57 Further studies are required to fully understand the roles of TNFα‐induced TNFR1 and TNFR2 signalling via JNK in paracetamol‐induced liver failure.
The protective effect of JNK inhibition in paracetamol‐induced liver injury was not translated into protection in other models of liver injury. JNK inhibition was not protective after Fas ligation and CCl4 injection, which confirms previous reports.23,55 Similar to paracetamol hepatotoxicity, TNFα has been implicated in CCl4‐mediated liver injury, but again conflicting published data exist. Injection of soluble TNF receptors limits CCl4 hepatotoxicity58; however, pre‐injection of anti‐TNF antibodies has no protective effect.59 Furthermore, studies of TNF receptor 1, 2 or double‐knockout or TNFα‐knockout mice have demonstrated either no effect or protection after CCl4‐mediated liver injury.60,61,62,63 In contrast with our data on JNK inhibition in the context of paracetamol hepatotoxicity, we observed no reduction in hepatic TNFα expression after JNK inhibition and CCl4 injection (data not shown).
JNK induces the expression of TNFα in several cell types.49,50,64,65,66 Transfection of liver‐specific macrophages (Kupffer cells) with constitutively active adenovirus expressing the upstream JNK‐kinase, MKK‐7, induces TNFα production, and JNK inhibition reduces leptin‐induced TNFα production in the same cell type.65 In view of our data, it is noteworthy that a functional redundancy of JNK genes in TNFα production from macrophages has been reported.64 We speculate that pan‐inhibition of the JNK signalling pathway down regulates TNFα expression by Kupffer cells and reduces paracetamol‐induced liver injury. However, this may be difficult to study in vivo as Kupffer cell‐depleted mice are protected from paracetamol‐induced hepatic necrosis.67 Therefore, future studies with conditional cell‐specific JNK knockouts in the liver may aid further analysis in this regard.
The liver expresses two JNK genes—JNK1 and JNK2. Although complete JNK inhibition by SP600125 and d‐JNKI1 was protective, liver injury was similar in JNK1−/− and JNK2−/− mice compared with WT mice. Others have recently reported partial limitation of paracetamol‐induced liver injury in JNK2−/− mice or in mice treated with JNK2 antisense RNA, but this protection was not as effective as pan‐JNK inhibition with either pharmacological inhibitors or JNK antisense RNA.55 The context and cell‐specific effects of JNK1 or JNK2 knockout on cell death have been reported. Our data suggest redundancy between the JNK1 and JNK2 signalling pathways in the context of paracetamol‐induced liver injury. We were unable to directly address this further in our study as JNK1−/−2−/− (double null) mice are embryonic lethal, and therefore future studies with conditional knockouts in the liver may aid further analysis of the relative roles of these genes in paracetamol‐induced hepatic necrosis.
In summary, we found a massive increase in hepatic JNK activity during paracetamol‐induced hepatic failure in a murine model. Inhibition of hepatic JNK with either a pharmacological or peptide inhibitor significantly reduced liver injury and mortality without affecting paracetamol bioactivation, and confirms and expands recently published data.55 The hepatoprotective effect of JNK inhibition may be by a specific reduction in hepatic TNFα expression. From a clinical standpoint, we have shown that JNK inhibition is more efficacious in reducing liver injury at later time points when the traditional antidote NAC is no longer effective. These data demonstrate that JNK plays a crucial role in hepatocyte death after paracetamol poisoning, and suggest that JNK inhibition may find clinical applications in patients who present late after overdose or in whom timing of the overdose is unclear.
We thank Kirsten Atkinson and Anne Pryde for expert technical assistance. This work was supported by the Scottish Hospital Endowments Research Trust (SHERT; Grant Number RG56/01), the Wellcome Trust, UK and a Lothian University Hospitals NHS Trust Research and Development grant (Grant Number PG2004/26).
ALF - acute liver failure
ALT - alanine aminotransferase
AP‐1 - activating protein 1
CCl4 - carbon tetrachloride
d‐JNKI1 - c‐jun N‐terminal kinase peptide inhibitor 1, d‐stereoisomer
IP - intraperitoneal
JNK - c‐jun (NH2) terminal kinase
NAC - N‐acetylcysteine
NAPQI - N‐acetyl‐p‐quinoneimine
PBS - phosphate‐buffered saline
ROS - reactive oxygen species
TNFα - tumour necrosis factor α
TNFR1 - TNF receptor 1
WT - wild type
Competing interests: None.