PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. Oct 1994; 176(19): 6134–6138.
PMCID: PMC196835
An Escherichia coli K-12 tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth.
G Zhao and M E Winkler
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston 77030-1501.
Abstract
We show that a tktA tktB double mutant, which is devoid of the two known transketolase isoenzymes of Escherichia coli K-12, requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth. This pyridoxine requirement can also be satisfied by 4-hydroxy-L-threonine or glycolaldehyde. These results provide direct evidence that D-erythrose-4-phosphate is a precursor of the pyridine ring of pyridoxine. In addition, they show that the two major E. coli transketolase isoenzymes are not required for the biosynthesis of D-1-deoxyxylulose, which is thought to be another precursor of pyridoxine.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (843K), or click on a page image below to browse page by page.
Articles from Journal of Bacteriology are provided here courtesy of
American Society for Microbiology (ASM)