PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. Aug 1994; 176(16): 4816–4824.
PMCID: PMC196315
Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation- and stress-induced proteins.
M Givskov, L Eberl, and S Molin
Department of Microbiology, Technical University of Denmark, Lyngby.
Abstract
The responses of Pseudomonas putida KT2442 to various forms of nutrient starvation and stress conditions were examined by two-dimensional polyacrylamide electrophoresis. Carbon deprivation resulted in a temporal expression of two classes of starvation-induced proteins: one class was transiently expressed during the initial phase of starvation, and the second class was expressed throughout the entire starvation period. Proteins of the second class could be further subdivided into proteins induced specifically under conditions of carbon starvation, proteins also induced by conditions of stress created by elevated temperature and osmolarity, and finally proteins that were also induced by conditions of nitrogen as well as phosphate starvation. Addition of glucose to a carbon-starved culture led to initiation of a recovery phase. During this phase, repression of starvation-induced proteins as well as induction of a new class of transiently expressed proteins, referred to as maturation proteins, took place.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.6M), or click on a page image below to browse page by page.
Images in this article
Click on the image to see a larger version.
Articles from Journal of Bacteriology are provided here courtesy of
American Society for Microbiology (ASM)