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Ann Rheum Dis. 2007 September; 66(9): 1263–1264.
PMCID: PMC1955137

Protective effect of A at position –168 in the type III promoter of the MHCIITA gene in systemic lupus erythematosus

The MHC class II transactivator (MHCIITA) gene was originally identified as a defective gene associated with bare lymphocyte syndrome, a severe combined immunodeficiency syndrome. Evidence indicates that MHCIITA is a master switch of antigen presentation in antigen‐presenting cells.1 A recent paper describes how a –168A ‐>G polymorphism in the type III promoter of the MHC2TA gene was associated with increased susceptibility to rheumatoid arthritis, multiple sclerosis and myocardial infarction, as well as lower expression of MHC2TA after stimulation of leukocytes with interferon‐gamma (IFN‐γ).2,3 However, similar replicated studies in two other European populations did not find this association.4,5

Systemic lupus erythematosus (SLE) is an autoimmune disease that is characterized by a complex set of immunological abnormalities that result in dysfunction of several organs. It has been reported that enhanced MHC class II antigen expression occurs in lupus nephritis in human and mice models.6,7 Therefore, particular alleles of the polymorphic gene involved in the regulation of MHC class II expression might be involved in the predisposition to SLE. We report here the association between a –168A ‐>G polymorphism in the human MHC2TA gene and SLE. DNA samples were obtained from 180 Japanese patients with SLE (average age at onset: 26.4 ± 11.7 years) and 175 Japanese healthy controls. All the patients with SLE fulfilled the American College of Rheumatology criteria for SLE.8 Informed consent was obtained from each individual in this study. Genomic DNA was isolated from peripheral blood mononuclear cells. Genotypes of –168A ‐>G (rs3087456) were determined by a TaqMan fluorogenic 5′ nuclease assay (Applied Biosystems, Tokyo, Japan) as described elsewhere.9 Association between SLE susceptibility and –168A ‐>G polymorphism was estimated by the Chi‐square test that was implemented in R software package version 2.0.1 (http://www.r‐project.org/).

As shown in table 11,, we found a significant difference in allele frequencies for the single nucleotide polymorphism (SNP; –168A ‐>G) in individuals with SLE (p = 0.008). The presence of G at position –168 was frequent in normal Japanese individuals, in contrast to the study by Swanberg et al.2 In SLE patients, A at position –168 was significantly associated with a decreased risk of developing SLE (p = 0.006; OR = 1.91). However, G at position –168 was not associated with an increased risk of developing SLE (p = 0.497). Therefore, we concluded that –168 A dominance is protective against the occurrence of SLE.

Table thumbnail
Table 1 Distribution of MHC2TA –168A ‐>G in patients and controls

Upregulation of CIITA is reported to direct T cells differentiating to the Th1 but not to the Th2 lineage.10 The expression of MHC2TA mRNA has been shown to be lower in cells from individuals with genotype GG than in cells from individuals with other genotypes, by activation with IFN‐γ (2). If that is the case, –168 A dominance might be associated with high expression of MHCIITA, resulting in the predominance of Th1 lineage over Th2, and this phenotype might be associated with protection against the occurrence of SLE. Further studies are needed to confirm the biological effects of SNP (–168A ‐>G) in association with the differentiation of Th1 and Th2 lineage cells and also to study its role in the pathogenesis of SLE.

Abbreviations

IFN‐γ - interferon‐gamma

MHCIITA - MHC class II transactivator

SLE - systemic lupus erythematosus

Footnotes

Competing interests: None declared.

References

1. Ting J P, Trowsdale J. Genetic control of MHC class II expression. Cell 2002. 109S21–S33.S33 [PubMed]
2. Swanberg M, Lidman O, Padyukov L, Eriksson P, Akesson E, Jagodic M. et al MHC2TA is associated with differential MHC molecule expression and susceptibility to rheumatoid arthritis, multiple sclerosis and myocardial infarction. Nat Genet 2005. 37486–494.494 [PubMed]
3. Iikuni N, Ikari K, Momohara S, Tomatsu T, Hara M, Yamanaka H. et al MHC2TA is associated with rheumatoid arthritis in Japanese patients. Ann Rheum Dis 2007. 66274–275.275 [PMC free article] [PubMed]
4. Yazdani‐Biuki B, Brickmann K, Wohlfahrt K, Mueller T, Marz W, Renner W. et al The MHC2TA –168A>G gene polymorphism is not associated with rheumatoid arthritis in Austrian patients. Arthritis Res Ther 2006. 8R97
5. Akkad D A, Jagiello P, Szyld P, Goedde R, Wieczorek S, Gross W L. et al Promoter polymorphism rs3087456 in the MHC class II transactivator gene is not associated with susceptibility for selected autoimmune diseases in German patient groups. Int J Immunogenet 2006. 3359–61.61 [PubMed]
6. Cheah P L, Looi L M, Chua C T, Yap S F, Fleming S. Enhanced major histocompatibility complex (MHC) class II antigen expression in lupus nephritis. Malays J Pathol 1997. 19115–120.120 [PubMed]
7. Yokoyama H, Takabatake T, Takaeda M, Wada T, Naito T, Ikeda K. et al Up‐regulated MHC‐class II expression and gamma‐IFN and soluble IL‐2R in lupus nephritis. Kidney Int 1992. 42755–763.763 [PubMed]
8. Tan E M, Cohen A S, Fries J F, Masi A T, McShane D J, Rothfield N F. et al The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982. 251271–1277.1277 [PubMed]
9. Ikari K, Momohara S, Inoue E, Tomatsu T, Hara M, Yamanaka H. et al Haplotype analysis revealed no association between the PTPN22 gene and RA in a Japanese population. Rheumatology 2006. 451345–1348.1348 [PubMed]
10. Gourley T, Roys S, Lukacs N W, Kunkel S L, Flavell R A, Chang C H. A novel role for the major histocompatibility complex class II transactivator CIITA in the repression of IL‐4 production. Immunity 1999. 10377–386.386 [PubMed]

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