PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
 
Appl Environ Microbiol. 1992 February; 58(2): 671–676.
PMCID: PMC195300

Total Degradation of EDTA by Mixed Cultures and a Bacterial Isolate

Abstract

A bacterial mixed culture, which was obtained from sewage by a special enrichment procedure, utilized EDTA as the sole source of carbon and nitrogen for growth. High concentrations of mineral salts, particularly CaCl2, or the use of a mineral base without nitrogen protected the cells from inactivation after transfer into fresh medium containing 200-mg/liter (0.67 mM) EDTA. The chemical speciation did not influence the biodegradability of EDTA. However, when resting cells of the mixed culture were incubated with EDTA in the presence of an equivalent molar amount of FeCl3, the reaction came to a halt before the complete consumption of the substrate. A gram-negative isolate from the mixed population, BNC1, also metabolized EDTA in monoculture. Growth of the pure culture was promoted by biotin or folic acid but was always accompanied by the accumulation of unidentified metabolites and was slow (μmax, 0.024 h-1) compared with that of the original community (μmax, 0.036 h-1).

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cripps RE, Noble AS. The metabolism of nitrilotriacetate by a pseudomonad. Biochem J. 1973 Dec;136(4):1059–1068. [PubMed]
  • EAGON RG, CARSON KJ. LYSIS OF CELL WALLS AND INTACT CELLS OF PSEUDOMONAS AERUGINOSA BY ETHYLENEDIAMINE TETRAACETIC ACID AND BY LYSOZYME. Can J Microbiol. 1965 Apr;11:193–201. [PubMed]
  • Egli T. (An)aerobic breakdown of chelating agents used in household detergents. Microbiol Sci. 1988 Feb;5(2):36–41. [PubMed]
  • Firestone MK, Tiedje JM. Pathway of degradation of nitrilotriacetate by a Pseudomonas species. Appl Environ Microbiol. 1978 May;35(5):955–961. [PMC free article] [PubMed]
  • Focht DD, Joseph HA. Bacterial degradation of nitrilotriacetic acid (NTA). Can J Microbiol. 1971 Dec;17(12):1553–1556. [PubMed]
  • Genthner BR, Davis CL, Bryant MP. Features of rumen and sewage sludge strains of Eubacterium limosum, a methanol- and H2-CO2-utilizing species. Appl Environ Microbiol. 1981 Jul;42(1):12–19. [PMC free article] [PubMed]
  • Gerike P, Fischer WK. A correlation study of biodegradability determinations with various chemicals in various tests. Ecotoxicol Environ Saf. 1979 Jun;3(2):159–173. [PubMed]
  • Lauff JJ, Steele DB, Coogan LA, Breitfeller JM. Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp. Appl Environ Microbiol. 1990 Nov;56(11):3346–3353. [PMC free article] [PubMed]
  • Madsen EL, Alexander M. Effects of chemical speciation on the mineralization of organic compounds by microorganisms. Appl Environ Microbiol. 1985 Aug;50(2):342–349. [PMC free article] [PubMed]
  • SCHMIDT K, LIAAENJENSEN S, SCHLEGEL HG. DIE CAROTINOIDE DER THIORHODACEAE. I. OKENON ALS HAUPTEAROTINOID VON CHROMATIUM OKENII PERTY. Arch Mikrobiol. 1963 Aug 1;46:117–126. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)