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Over the last seven years our laboratory has focused on the determination of the structural aspects of nucleoside triphosphate diphosphohydrolases (NTPDases) using site-directed mutagenesis and computational comparative protein modeling to generate hypotheses and models for the hydrolytic site and enzymatic mechanism of the family of NTPDase nucleotidases. This review summarizes these studies utilizing NTPDase3 (also known as CD39L3 and HB6), an NTPDase family member that is intermediate in its characteristics between the more widely distributed and studied NTPDase1 (also known as CD39) and NTPDase2 (also known as CD39L1 and ecto-ATPase) enzymes. Relevant site-directed mutagenesis studies of other NTPDases are also discussed and compared to NTPDase3 results. It is anticipated that many of the results and conclusions reached via studies of NTPDase3 will be relevant to understanding the structure and enzymatic mechanism of all the cell-surface members of this family (NTPDase1–3, 8), and that understanding these NTPDase enzymes will aid in modulating the many varied processes under purinergic signaling control. This review also integrates the site-directed mutagenesis results with a recent 3-D structural model for the extracellular portion of NTPDases that helps explain the importance of the apyrase conserved regions (ACRs) of the NTPDases. Utilizing this model and published work from Dr Guidotti’s laboratory concerning the importance and characteristics of the two transmembrane helices and their movements in response to substrate, we present a speculative cartoon model of the enzymatic mechanism of the membrane-bound NTPDases that integrates movements of the extracellular region required for catalysis with movements of the N- and C-terminal transmembrane helices that are important for control and modulation of enzyme activity.
In this section site-directed mutagenesis studies introducing point mutations in NTPDases are reviewed. Other manipulations of protein structure including chimeras and truncation mutants of NTPDases are not covered in this review.
Over the last seven years, 10 studies have been published using point mutations introduced by site-directed mutagenesis to investigate the structure and function of human NTPDase3 (see Table Table1).1). What follows is a concise summary of those studies, in chronological order.
The first report of site-directed mutagenesis of any NTPDase  was important for establishing experimentally what had been hypothesized based on limited sequence homology of the NTPDases with the actin/heat shock protein/sugar kinase superfamily of proteins. Specifically, the conservation of the two ‘DXG’ phosphate binding motifs of the actin superfamily in the NTPDases suggested that these regions serve the same function in the NTPDases as they do in the actin family. The importance of these residues in ACR1 and ACR4 in the NTPDases was established by mutation of these aspartic acids and glycines, resulting in the inactivation of the enzyme without a change in the protein expression level.
The significance of the two tryptophan residues that are most highly conserved in the NTPDases was established through the mutation of W187 and W459 in NTPDase3 . Mutation of W187 in ACR3 resulted in inactivation, while mutation of W459 in ACR5 resulted in an active enzyme with increased ATPase activity, and therefore an increased ATPase:ADPase hydrolysis ratio. Coupling this W459A mutation with the D219E mutation in DXG2/ACR4 resulted in near conversion of NTPDase3 from an apyrase to an ecto-ATPase (i.e., increased dramatically the NTPase: NDPase hydrolysis ratio for most nucleotides), indicating that a major change in nucleotide tri-versus di-phosphatase specificity is possible with only a few changes in amino acid sequence.
Mutation of several conserved amino acids located in ACR1–4 established the importance of these regions and some of these residues for enzymatic activity of NTPDase3 . The N191A mutation, located in ACR3, changed the ATPase:ADPase ratio to a similar value as exhibited by NTPDase1 (approximately 1.0). Mutation of a serine residue in ACR4 (S224A) resulted in complete inactivation of enzyme activity. Mutation of a glutamic acid in ACR3 (E182) to either aspartic acid or glutamine also caused complete inactivation of activity. However, differences were noted in the two mutations at this site, with the E182D mutant incapable of binding to the nucleotide analogue, Cibacron blue, as well as being more susceptible to limited proteolysis, while the E182Q mutant behaved more like wild-type NTPDase3 in these assays. These results, along with a lower expression level for the E182D mutant, suggest that this residue in ACR3 may be important both for hydrolysis of substrate and for proper folding of the protein. This is consistent with the location of ACR3 in the 3-D computational model of the extracellular portion of NTPDase3 shown in Figure Figure1B1B and Figure Figure22.
Several conserved histidine residues were mutated in NTPDase3 . This study was initiated since it had been previously noted that a chemical modification reagent that is selective for histidine, diethylpyrocarbonate (DEPC), was one of the few amino acid selective modification reagents that inhibited the NTPDases [5, 6]. Surprisingly, no conserved histidine residue was found to be essential for activity or to be the site of DEPC induced inhibition. However, H135 was found to be important for full activity, and mutagenesis of H257 resulted in greater sensitivity to DEPC inactivation. The R67H mutation, which makes NTPDase3 more like the other NTPDases, which have a histidine in this position in ACR1, increased the sensitivity to inactivation by DEPC. This implies that, in other NTPDases, this histidine in ACR1 may be a major site of modification by DEPC causing inactivation, as was suggested by Dzhandzhugazyan and Plesner  for NTPDase1/CD39, based on its conservation in NTPDases other than NTPDase3. Interestingly, simultaneous replacement of all three of the most highly conserved histidine residues in NTPDase3 caused the formation of large aggregates, without inactivating the enzyme, suggesting that these histidines may be important for higher order oligomeric formation or stability.
The most highly conserved glycine residues (excluding the 2 DXG glycines mutated in NTPDase3 discussed above ) were mutated to alanine . One rationale for making these mutations was the observation that several conserved glycines were important for the structure and function of the actin superfamily of proteins [9, 10]. Indeed, several such residues were found to be important for NTPDase3 activity (see Table Table1).1). In addition, two of the mutants, G263A and G462A, resulted in moderate to severe problems in folding and/or processing of NTPDase3. This study was also significant for the identification of two additional ACR regions (each containing a conserved glycine residue), termed ACR1a and ACR4a. These regions were identified by objective and quantitative computer analyses of the multiple sequence alignment for all the NTPDase sequences available at that time.
The significance of the three non-extracellular cysteine residues, assumed to exist as free sulfhydryls in NTPDase3, were investigated by mutation to serine . C10, located in the N-terminal cytoplasmic tail region, was found to be responsible for the covalent dimer formation that occurs by spontaneous oxidation of that residue during membrane preparation. This C10S mutant would be used in several subsequent studies as a replacement for the wild-type NTPDase3, since the C10S mutant has wild-type like activity and characteristics, but does not spontaneously form covalent dimers, which obscures the interpretation of some experiments. C501, located in the C-terminal membrane spanning (MS) helix of NTPDase3, was found to be the site of labeling by p-chloromercuriphenylsulfonic acid (pCMPS) that results in partial enzyme inactivation. This was somewhat unexpected, since C501 is predicted to be located almost in the middle of the C-terminal transmembrane (TM) domain, and should therefore be inaccessible to this charged reagent. This suggested the possibility of an aqueous pore or crevice, allowing pCMPS to reach C501. This finding is consistent with more recent results published by Grinthal and Guidotti , suggesting that the extracellular portions of the TM helices are more closely associated with one another and have less flexibility than the cytoplasmic portions of the same transmembrane helices (see below). Thus, it seems likely that the pCMPS reagent accesses C501 via the cytoplasmic face of the membrane rather than the extracellular face of the membrane (see Figure Figure33).
The role of glycosylation at the most conserved glycosylation site of the NTPDases, which is located between ACR1 and ACR1a, was established by mutation of N81 and T83 in NTPDase3 . Mutation of either the asparagine residue where the sugars are attached (N81D and N81E) or the threonine residue that is part of the consensus sequence for glycosylation at that site (T83A) resulted in decreased nucleotidase activities. The ATPase activity was more severely affected than the ADPase activity, and the Mg2+ activity was more severely decreased than the Ca2+ activity, suggesting that the mutations might be decreasing activity via oligomeric structure effects. Loss of inhibition by a deglycosylation enzyme (PNGase-F) and the lack of stimulation/stabilization of activity by the lectin, Concanavalin A, were also observed in these mutants.
The importance of lysine residue 79 located near the conserved glycosylation site near ACR1 was investigated . This residue is conserved in cell surface NTPDases (NTPDase1–3, 8), but not in the soluble (NTPDase5–6) and intracellular (NTPDase4, 7) members of this nucleotidase family (see Table Table11 in ). All substitutions at this site resulted in a loss of 70%–80% of activity. Only K79R, which alone maintains the positive charge at this residue, remained able to be stimulated/stabilized by the lectin Con A (presumably via binding at the nearby N81 glycosylation site ). The residual activity of all the mutants at the K79 site became resistant to the inhibitory effects of the detergent, Triton X-100. Since this detergent was shown to dissociate the NTPDases into monomers (presumably its mechanism of inhibition of activity [11, 15]), it was postulated that these K79 mutants might have weakened oligomeric structures, accounting for their lower activity and insensitivity to Triton X-100. It was also hypothesized that the reason for the activity of these mutants being higher in Ca2+ than in Mg2+ might be due to a subtle stabilization of tertiary or quaternary structure by Ca2+, partially correcting the destabilization of these mutants caused by substitution at K79.
The residues responsible for the increase in activity observed upon acetylation of NTPDase3 with the tyrosine selective chemical modification reagent, N-acetylimidazole (N-AI) were established by site-directed mutagenesis . N-AI modification resulted in a larger increase in ATPase (approximately 2-fold) than ADPase (approximately 1.4 fold) activity in wild-type NTPDase3. However, this reagent did not increase the activity of other NTPDases tested. In NTPDase3, mutation of Y252 (in ACR4a) did not negatively affect activity, but abolished the N-AI induced increase in ATPase and ADPase activity. This effect of N-AI was also abolished by prior solubilization with the monomerizing detergent Triton X-100, and reversed by subsequent treatment with Triton X-100, suggesting that the effect of N-AI modification is dependent upon changes in the tertiary and/or quaternary structure which result in a more stable oligomeric structure with more stable enzyme activity. In addition, the mutations that abolished the N-AI induced increase in nucleotidase activity also diminished the increase in activity observed after treatment with Con A or a chemical cross-linker. These observations, coupled with the nearby location of a computer predicted  helix-loop-helix dimerization domain (238NTSDOMQVS246) lead to the hypothesis that this region near ACR4a may be important for the oligomeric structure and stability of NTPDase3.
Site directed mutagenesis of the 10 conserved extracellular cysteines (which presumably form five conserved disulfide bonds in all the cell-surface NTPDases) established the location of the five extracellular disulfide bonds in NTPDase3 (, see Figure Figure1A).1A). Unlike soluble NTPDase6, whose two disulfide bonds were determined by classic protein chemistry techniques , the membranous NTPDases are not amenable to this direct approach. Therefore, the disulfides were assigned by analysis of the enzymatic phenotypes of all the single and double extracellular cysteine mutants of NTPDase3 . Thus, the expressed cysteine mutant proteins were analyzed for nucleotidase activity, glycosylation state and sensitivity to Endo H, delivery to the cell surface, and susceptibility to inactivation by reduction, in order to deduce the disulfide bond architecture. Loss of each of the five disulfide bonds had distinct effects on enzyme activity, processing, and structure (see Table Table11 and ).
The same paper that examined the disulfide bond structure of NTPDase3 also generated three-dimensional models of NTPDase3 (and other NTPDases) via homology modeling of the NTPDase3 extracellular sequence . A high degree of structural fold similarity between the NTPDases and a bacterial exopolyphosphatase (PDB 1T6C) was observed . The resultant theoretical 3-D models of the extracellular portion of NTPDase3 (see  and the discussion below), based on homology with this exopolyphosphatase, were consistent with the assignment of the disulfide bonds occurring in regions of good fold similarity between NTPDase3 and the exopolyphosphatase. The 3-D models also suggested the structural basis for the importance of several apyrase conserved regions of the NTPDases (see Figure Figure1B1B).
Several site-directed mutagenesis studies (as well as other manipulations of the protein structure) using NTPDase1 (CD39) have been published by Dr Guidotti’s group. One early example is the work demonstrating the importance of a histidine residue (H59) in ACR1 of rat brain NTPDase1  (this residue is R67 in rat, mouse, and human NTPDase3). The more recent studies from the Guidotti laboratory have focused on the importance of the presence and motility of the two transmembrane helices in rat brain NTPDase1/CD39 that are common to all the cell-surface NTPDases [12, 15, 20, 21], as well as the significance of glycosylation for NTPDase1 processing and function [22, 23]. Since Dr Guidotti is also contributing an article to this special issue on nucleotidases, most of his laboratory’s work in this field will not be discussed here. However, one recent study from his laboratory  is of particular relevance to this review. In that study, the authors introduced cysteines into the transmembrane (TM) domains of rat brain CD39/NTPDase1 and used a disulfide cross-linking strategy to investigate TM helix interactions and dynamics (and their functional consequences). They concluded that strong interactions exist between the regions of the TM domains nearest the extracellular side of the membrane, while only weak interactions exist near the cytoplasmic side of the TM domains. They also presented evidence that NTPDase1 activity is dependent on the ability of these two TM helices to move relative to one another.
Dr Marcus’s group has also published mutagenesis studies of CD39/NTPDase1. They mutagenized conserved amino acids in ACR1-4 in a soluble construct derived from human CD39/NTPDase1 . Some of the mutations made in that study are homologous to the residues mutated in NTPDase3 (see Tables Tables11 and and2).2). They concluded that NTPDase1 E174 (equivalent to E182 in NTPDase3) and S218 (equivalent to S224 in NTPDase3) are essential for the enzymatic and biological (inhibition of ADP-induced platelet aggregation) activities of solCD39/NTPDase1. They also noted that the S57A mutation in ACR1 caused a two-fold increase in ADPase activity, but no change in ATPase activity, and that the Y127A mutation in ACR2 lost about 50% ADPase and ATPase activities. In addition, differences in preference for Ca2+versus Mg2+ were noted for some mutants.
The aspartic acid residues constituting the ‘D’ residues in both ‘DXG’ motifs in a soluble form of human CD39/NTPDase1 (D54 and D213, corresponding to D62 and D219 in NTPDase3) were mutated by Drosopoulos . In contrast to our laboratory’s results using NTPDase3 (see , Table Table1),1), mutation of these residues did not cause nearly total inactivation of NTPDase1 ATPase and ADPase activities, using either Ca2+ or Mg2+ as co-substrate. Only when both residues were mutated was the activity completely abolished. Kinetic analyses of the single mutants revealed changes in Km for Ca2+ and nucleotides. It was concluded that these two residues in the DXG regions are involved in Ca2+ utilization, and possibly involved in coordination of the Ca2+ used as co-substrate with the nucleotide. Our laboratory has re-examined these homologous mutations in NTPDase3 trying to mimic assay conditions used by Drosopoulos, and we have obtained basically the same results as were previously published  — in NTPDase3, these mutants are basically inactive using either calcium or magnesium as co-substrate, using a variety of nucleotide concentrations and assay buffer compositions. Thus, it is possible that there may be some real differences in the roles of the DXG motifs between the membrane-bound NTPDase3 and the soluble NTPDase1 used in these two seemingly partially contradictory studies. Interestingly, we note that the amino acid residues homologous to NTPDase ACR4 form a Ca2+ binding site in the crystal structure of the bacterial exopolyphosphatase (PPX/GPPA ) used as a template for construction of the computational model of the 3-D structure of the NTPDases (see below).
Mateo et al.  deduced the importance of C399 and N443 in human NTPDase2/ecto-ATPase while studying splice variants of this ecto-ATPase. The C399S mutant resulted in a totally inactive protein, while the N443D mutant, which removes a potential glycosylation site, had reduced activity. Those authors concluded that both these mutations reduced enzyme activity via their detrimental effects on the folding and processing of these proteins, presumably explaining why, of the three splice mutants of human NTPDase2 examined, only the longest protein (495 amino acids) has any nucleotidase activity. They further deduced that the mutation of C399 most likely resulted in an inactive enzyme due to disruption of a disulfide bond necessary for maintaining the structure of the enzyme. This result and conclusion is consistent with our laboratory’s findings that the cysteine residue corresponding to C399 in NTPDase2 is indeed disulfide bonded, both in soluble NTPDase6 (equivalent to C354 in NTPDase6, in the disulfide bond C340–C354 ), and in NTPDase3 (equivalent to C422 in NTPDase3, in the 5th disulfide bond C399–C422 ), and that this disulfide bond is essential for processing and activity in NTPDase3 .
The location of the two disulfide bonds in the soluble NTPDase6 were determined by direct protein chemistry techniques . Unfortunately, this direct approach is not feasible for the cell-surface localized NTPDase3, due to the higher complexity (5 versus 2 disulfide bonds) and lack of sufficient amounts of purified protein. Thus, we were forced to establish likely disulfide bonds using an indirect mutagenesis approach . Even though the disulfide bonds were determined indirectly, the disulfide bond assignments made are likely to be correct due to the existence of several types of data that are all consistent with one another and with the assignments made , and they are likely to be present in all the cell surface NTPDases, since these 10 extracellular cysteines are conserved. The disulfide bond assignments are schematically indicated in Figure Figure1A.1A. We then used this information to help evaluate a computational model of the 3-D structure of NTPDase3, which was obtained by threading the amino acid sequences of the extracellular portions of several NTPDases through a protein database, using fold recognition software (the FUGUE and FFAS programs, ). Only one protein was found to have very good fold similarity to the NTPDases, a bacterial exopolyphosphatase (PPX/GPPA). A two dimensional representation of the NTPDase3 3-D model based on the PPX/GPPA template structure is shown in Figure Figure1B1B.
As objectively evaluated by the program Verify3D “http://www.doe-mbi.ucla.edu/Services/Verify_3D/ ), some parts of the resulting 3-D FUGUE “http://wwwcryst. bioc.cam.ac.uk/fugue/ ) NTPDase3 model are more likely to be valid than others. Thus, the N-terminal lobe of the molecule, containing ACR1, ACR1a, and ACR2, as well as the C92–C116 disulfide bond, is likely to be close to the real structure of NTPDase3. In addition, ACR3, ACR4 and the region immediately preceding ACR5 are also predicted to be well modeled by the Verify3D program (see Supporting Information file # 3 in ). However, there is a region of the protein after ACR4 and before ACR5 where the template protein (bacterial exopolyphosphatase (PPX/GPPA)) differs in predicted secondary structure and predicted folding from the NTPDases. This region includes C261 and C308, which are disulfide linked, but are not near each other in the model (see Figure Figure1B).1B). This is in contrast to C92/C116 and C399/C422, which are close together in the model, as would be expected, since they are disulfide linked. Note that four cysteine residues in NTPDase3 (C289, C334, C347, and C353) are not represented in this model, since they have no corresponding residues in the PPX/GPPA template protein and therefore are not included in the model shown in Figure Figure1B1B (see ).
Fold recognition searches using the sequence of human NTPDase3 between ACR4 and ACR5 (from approximately residues 240–380, including the four cysteine residues not represented in the above model, which sequence was computationally predicted to form an independent domain ) as a query sequence revealed a number of weak fold similarity matches to a tyrosine phosphatase and other structurally related proteins. Although this domain’s fold similarity match was significantly worse than that found for the entire NTPDase3 molecule and the PPX/GPPA protein, a LOOPP (http://cbsu.tc.cornell.edu/software/loopp/ ) generated 3-D model of this putative domain of NTPDase3 based on a bacterial tyrosine phosphatase template (PDB code 1P8A) places the six NTPDase3 cysteines that exist in this part of NTPDase3 in positions consistent with the second (C261–C308), third (C289–C334), and fourth (C347–C355) disulfide bonds determined for NTPDase3 (see Figure Figure1C).1C). Thus, the identified disulfide structure aids in the evaluation of the generated 3-D models of NTPDase3, and will be instrumental in further refinements and validation of these and other models.
The NTPDase3 model suggests the structural basis for the importance of several ACRs (see Figure Figure1B).1B). ACR1 and ACR4 are each represented by a β-hairpin loop located on either side of a large cleft forming the active site (the highly conserved DXG sequences within ACR1 and ACR4 are depicted in blue space filling mode). ACR3 and ACR5 precisely correspond to two a-helices that connect the N- and C-domains (‘lobes’). Such a structure would allow substantial rotational movements of these two extracellular domains/lobes relative to one another. This motion has been described for the enzymes of the actin superfamily as a ‘butterfly-like’ opening of the active site cleft involved in catalysis [31, 32].
The NTPDase3 computational model is also consistent with the site-directed mutagenesis studies listed in Tables Tables11 and and2.2. In Figure Figure2,2, we highlight the mutations of NTPDase3 that cause nearly complete inactivation of nucleotidase activity (represented in red space-filling mode), in the context of the computational 3-D model of the extracellular portion of NTPDase3 and the ACR regions (represented as color-coded ribbons). The location of most of these inactivating mutations are clustered around the putative active site cleft formed by the two lobes of the theoretical structure. The two putative phosphate binding domains (DXG sequences), which are part of ACR1 and ACR4 beta sheet structures (in blue) are located on either side of the active site cleft. ACR3 (green ribbon) and ACR5 (yellow ribbon) each contain ‘an essential’ residue identified by mutagenesis, and constitute the alpha helices which connect the N-terminal and C-terminal lobes of the extracellular domain. ACR2 is shown in orange (behind ACR1 in Figure Figure2),2), and is part b-sheet and part a-helix. Two other ACR regions identified by quantitative multiple sequence alignments (ACR1a and ACR4a , in purple) map to the top of the two lobes of the structure, removed from the active site. ACR4a contains G263, mutation of which was found to completely inactivate the enzyme, apparently via its negative effects on protein folding and/or processing . This region also contains C261, part of the second conserved disulfide bond in NTPDase3 . Elimination of this bond by mutation of this residue not only causes substantial inactivation of activity, but also makes the small amount of residual activity very sensitive to DTT-induced inactivation. This suggests that this bond and region are important for the integrity of this part of the structure, and that absence of this disulfide bond opens up the C-terminal lobe to reduction of other disulfides in the structure, whose reduction by DTT causes inactivation of the residual NTPDase3 activity .
Thus, the computational model and the mutagenesis results are consistent with one another and both support a functional homology of the NTPDases with the actin superfamily of proteins. Therefore, it is logical to assume that the ‘butterfly-like’ opening of the active site cleft involved in catalysis for the actin superfamily [31, 32] also exists in the NTPDases. In concordance with Grinthal and Guidotti , we also speculate that these putative movements of the two extracellular domain lobes in the integral membrane NTPDases are functionally coupled to movements of the N- and C-terminal membrane spanning helices described by those authors. Furthermore, we formalize and expand upon the textual model of Grinthal and Guidotti  by constructing the cartoon model presented in Figure Figure3,3, which incorporates several additional features not described previously . First, we incorporate our computational model of the 3-D structure of the extracellular region of the NTPDases (see Figures Figures1B1B and and2).2). Second, we note that there are conserved proline residues strategically located near the boundaries of the extracellular domain and the cell membrane, between the N-terminal TM helix and the ACR1 β-sheet, as well as between the C-terminal TM helix and the ACR5 α-helix (ACR1 and ACR5 are shown in red in Figure Figure3).3). We speculate that these prolines (represented by ‘P’ in Figure Figure3)3) may be important for providing the proper conformation and rigidity of structure needed for linking movements of the extracellular lobes (via ACR1 and ACR5) to movements of the transmembrane helices, and vice versa. These coordinated movements mediated through these conserved proline residues are speculated to be essential for full enzymatic activity. Thus, our cartoon model envisions the movement of the N- and C-terminal TM helices as being structurally and functionally linked to the movement of the N- and C-terminal lobes of the computational 3-D structure of the extracellular portion of NTPDase3, in agreement with an earlier report . Third, this speculative cartoon model of NTPDase3 (Figure (Figure3)3) is consistent with our previous study showing that C501, located in the Cterminal transmembrane domain, is accessible to the membrane impermeant reagent pCMPS, and that this modification reduces NTPDase3 activity . The linked, coordinated movements of the TM helices and the extracellular domains permit the ACR1 and ACR4 regions to move into close contact with the substrate to allow catalysis (middle image in Figure Figure3),3), and then reopen to permit the release of product and a new cycle of hydrolysis to begin. Since these functionally important movements of the TM helices described for CD39/NTPDase1  are very likely to occur in all the cell surface NTPDases (NTPDase1–3 and 8), and computational modeling of all the extracellular regions of the NTPDases (NTPDase1–3, 5–6, 8) gave rise to very similar predicted structures to that structure shown in Figure Figure1B1B for NTPDase3 (see Supporting Information file named NTPDase_FUGUE_Models.jpg in reference ), we propose that the speculative model depicted in Figure Figure33 is applicable to all the cell surface localized members of the NTPDase family.
There are several future approaches for advancing our knowledge of the structure of the NTPDases and how their structures determine their functions. First, there is a need for refinement and experimental verification of the PPX/GPPA derived NTPDase 3-D computational models. Second, such a refined model based on the PPX/GPPA protein can then be combined with a second model which better approximates the structure of the region between ACR4 and ACR5 (based on a protein tyrosine phosphatase?) to generate a more accurate 3-D model of the entire extracellular region. Third, this combined model can then be used to design experiments for the purpose of elucidating the contact surfaces of the extracellular regions between NTPDase monomers that are important for oligomer formation and stability, and therefore for function. Fourth, the possibility that the NTPDase ACR4 region functions as a Ca2+ binding site, which is suggested by the PPX/GPPA crystal structure and by the human solCD39 D213 mutagenesis results , should be experimentally addressed. Fifth, crystallization and X-ray diffraction analysis and/or NMR structural determination of the naturally soluble form of NTPDase6 or some construct consisting of the extracellular region of another NTPDase can be used to experimentally validate any models developed, and will aid in the interpretation of all mutagenesis studies. Finally, the speculative role of the conserved proline residues near the boundaries between the N- and C-terminal transmembrane helices and the extracellular domain (see Figure Figure3)3) should be experimentally addressed as part of a larger effort to elucidate the role played by the proposed interconnected movements of the extracellular and transmembrane domains for the enzymatic mechanism and modulation of the oligomeric, integral membrane protein NTPDases.
This work was supported by NIH grants HL59915 and HL72882 to T.L.K.