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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nat Clin Pract Neurol. Author manuscript; available in PMC 2007 July 31.
Published in final edited form as:
PMCID: PMC1936221

A patient with encephalitis associated with NMDA receptor antibodies



A 34-year-old woman presented with headache, feverish sensation and anxiety, rapidly followed by homicidal ideation, aggressive agitation, seizures, hypoventilation, hyperthermia and prominent autonomic instability requiring intubation and sedation. She developed episodes of hypotension and bradycardia with periods of asystole lasting up to 15 seconds. Upon weaning off sedation, her eyes opened but she was unresponsive to stimuli. There was muscle rigidity, frequent facial grimacing, rhythmic abdominal contractions, kicking motions of the legs, and intermittent dystonic postures of the right arm.


Routine laboratory testing, toxicology screening, studies for autoimmune and infectious etiologies, brain MRI scan, lumbar puncture, electroencephalogram, whole-body CT scan, abdominal ultrasound, paraneoplastic and voltage-gated potassium channel antibody serologies, analysis of N-methyl-D-aspartate receptor antibodies.


Paraneoplastic encephalitis associated with immature teratoma of the ovary and N-methyl-D-aspartate receptor antibodies.


Intensive care, mechanical ventilation, antiepileptics, laparotomy and left salpingo-oophorectomy, corticosteroids, plasma exchange, intravenous immunoglobulin, cyclophosphamide, physical therapy, and chemotherapy.

Keywords: catatonia, NMDA receptor antibodies, ovarian teratoma, paraneoplastic encephalitis, psychosis


For 1 week, a 34-year-old woman complained of headache, feeling feverish, and being unsure of herself. She attributed these symptoms to stress and anxiety and took two of her husband’s alprazolam pills one afternoon. The following day, she was found confused and was brought to the emergency room. Upon arrival she had generalized convulsions that were treated with lorazepam 4 mg intravenously and phenytoin 1,000 mg intravenously, and she was intubated for airway protection. Her temperature was 38.7°C; other vital signs, general examination, routine blood studies, and urine toxicology screening were normal. A head CT scan was unremarkable; cerebrospinal fluid (CSF) analysis showed a white blood cell count of 18 cells/ml (98% lymphocytes), red blood cell count of 26 cells/ml, glucose 4.27 mmol/l (77 mg/dl), and protein 0.55 g/l. Aciclovir 10 mg/kg body weight every 8 hours was started for possible herpes simplex virus (HSV) encephalitis. MRI fluid-attenuated inversion recovery (FLAIR) showed bilateral medial temporal lobe hyperintensity, predominantly involving the left hippocampus (Figure 1A). An electroencephalogram (EEG) showed 8–12 Hz mixed polymorphic alpha activity, without focal slowing, spikes or sharp waves. HSV polymerase chain reaction (PCR) was negative and aciclovir was discontinued. The patient’s mental status improved, and she was discharged home on levetiracetam 500 mg twice daily.

Figure 1
MRI scan of the patient at symptom presentation and follow-up. (A) MRI fluid-attenuated inversion recovery (FLAIR) obtained at symptom presentation demonstrates bilateral medial temporal lobe hyperintense signal, predominantly involving the left hippocampus ...

The following evening the patient awoke after having visions that she would stab and kill her 3-year-old son, and asked to be taken back to the hospital. On arrival, vital signs and neurologic examination were normal. She was tearful with pressured speech, and denied hearing voices or having suicidal ideations. She was diagnosed with acute psychosis and readmitted.

The patient’s medical history was notable for obesity and hyperglycemia, both attributed to a polycystic ovarian syndrome. She did not smoke, drink alcohol, or use illegal drugs. One sister had scleroderma and another had systemic lupus erythematosus.

Levetiracetam was discontinued and valproic acid loaded at 1,500 mg intravenously and then continued at 500 mg three times daily. CSF analyses were similar to those previously obtained; bacterial and fungal studies, cytology and flow cytometry were unrevealing. Tests for Lyme disease, Epstein–Barr virus, and arboviruses were negative. Aciclovir was restarted at the same dosage as previously, although a repeat HSV PCR was negative. The patient was given lorazepam 1–2 mg intravenously every 2 hours as needed and olanzapine 5 mg daily for aggressive agitation. Over the next few days she became less communicative, stopped following commands, and developed catatonic features. MRI showed persistent FLAIR hyperintensity in the hippocampi and mild increased meningeal enhancement. An EEG demonstrated 2–6 Hz polymorphic delta and theta activity, without epileptic activity. She developed episodes of hypoventilation, hypotension (around 80/30 mmHg), and bradycardia (30 beats per minute) with periods of asystole lasting up to 15 seconds. A transthoracic echocardiogram was normal, and the patient was transferred to the neurointensive care unit of another institution.

On arrival, the patient’s temperature was 39.4°C; she was intubated and sedated. Her sedation was immediately discontinued. Several hours later, she remained unresponsive; her eyes would open without tracking or blinking to threat. The patient’s pupils were reactive and her oculocephalic and gag reflexes were intact. Muscle tone was increased, with rigidity and normal reflexes. She had frequent facial grimacing, rhythmic abdominal contractions, kicking motions of the legs, and intermittent dystonic postures of the right arm. An EEG showed no epileptiform correlate to the motor activity, but because of concern for an occult epileptic focus, topiramate 100 mg twice daily was added. Testing for HIV, rapid plasma reagin, and a comprehensive panel for autoimmunity (including among others anti-double-stranded DNA, thyroid peroxidase, and antineutrophil cytoplasmic antibodies) were negative. CSF analy sis showed a white blood cell count of 30 cells/ml (97% lymphocytes), red blood cell count of 12 cells/ml, glucose 5.00 mmol/l (90 mg/dl), protein 0.58 g/l, IgG index 1.72 (normal 0.28–0.66), and three oligoclonal bands not present in serum. Repeat cytology and HSV PCR were negative, and aciclovir was discontinued.

A CT scan and pelvic ultrasound revealed a calcified hypodense lesion in the left adnexa (6.0 × 6.1 × 7.9 cm). Serum tumor markers were negative. Tests for paraneoplastic and voltage-gated potassium channel antibodies were negative, but antibodies to N-methyl-D-aspartate receptors (NMDARs) were identified in serum and CSF (Figure 2). Given the rapid deterioration of the patient’s condition, a left salpingo-oophorectomy was performed, revealing an immature teratoma of the ovary (Figure 3).

Figure 2
Demonstration of N-methyl-D-aspartate receptor antibodies in the patient’s cerebrospinal fluid. (A,B) Sagittal section of rat hippocampus incubated with the patient’s cerebrospinal fluid (CSF; diluted 1:20). The anti-N-methyl-D-aspartate ...
Figure 3
Histopathological studies of the patient’s teratoma. (AC) Areas of the tumor containing adipose tissue (a), epithelial tissue (e), and nervous tissue (n), all in the same section (panel A). Other areas contained choroid plexus (panel ...

The patient underwent tracheostomy and gastrostomy tube placement, and was started on intravenous methylprednisolone 1 g/day for 10 days and plasma exchange (5 exchanges in 10 days), followed by intravenous immunoglobulin (IVIg) 0.4 g/kg body weight/day for 5 days. As no improvement was noted, a bolus of cyclophosphamide was given (750 mg/m2). The patient continued on a slow steroid taper. One week later, the abnormal movements subsided and the cardiac arrhythmia resolved. She gradually became more alert and was weaned off the ventilator. She was severely deconditioned with minimal antigravity strength in all extremities. Language, cranial nerves and sensory function were intact. She did not remember the preceding 2 months, but could form new memories. Two months after discharge, follow-up MRI revealed considerable improvement of the FLAIR hyper-intensity in the hippocampi (Figure 1B). Her cognitive functions, memory, and psychiatric evaluation were normal. The neurological examination showed only mild generalized weakness; she could walk without assistance and had started chemotherapy.


The syndrome of this patient along with the MRI findings, CSF inflammatory features, and history of an ‘ovarian cyst’ raised suspicions of the presence of a type of paraneoplastic encephalitis that has recently been described in patients with teratoma.1 The disorder associates with antibodies to NMDAR and affects young women, who are then often hospitalized for psychiatric care with diagnoses of acute schizophrenia, catatonia, drug abuse, or malingering.2 It is not until the patient develops seizures, autonomic instability, dyskinesias or a decreased level of consciousness that an organic illness is considered. Less frequently, patients present with severe short-term memory deficits resembling limbic encephalopathy.3

Box 1 outlines the clinical features of this type of paraneoplastic encephalitis, which often evolves as follows: psychiatric or ‘limbic’ presentation with seizures, decline of consciousness, dysautonomia and movement disorder, and recovery after treatment.2,4,5 At times patients might remain with eyes open, mumbling or unresponsive, with increased muscle tone, similar to catatonia. In 25% of patients, FLAIR or T2 abnormalities in medial temporal or fronto-basal regions are observed, sometimes with mild or transient meningeal enhancement as occurred in the present case.2

Box 1

Clinical features and diagnostic tests in N-methyl-D-aspartate receptor antibody-associated encephalitis.

Psychiatric symptoms (frequent presentation)

Change of personality and behavior, irritability, anxiety, aggressive behavior, delusional thoughts, paranoia, catatonia

Short-term memory loss (rare presentation)

Similar to classical limbic encephalitis


Partial complex or generalized seizures

Autonomic instability

Hyperthermia (sometimes alternating with hypothermia), hypoventilation, fluctuations of blood pressure, tachycardia, bradycardia, constipation, ileus

Abnormal movements (usually without electroencephalogram evidence of epileptic activity)

Orofacial dyskinesias, dystonic posturing of the extremities, choreoathetoid movements, oculogyric crises, myoclonus, opisthotonos

Cerebrospinal fluid

Pleocytosis, increased protein concentration, normal glucose, oligoclonal bands and high IgG index


Frequently: focal or diffuse slow activity during episodes of dyskinesias or abnormal movements

Sometimes: epileptic activity


Frequently: small areas of FLAIR abnormalities in cerebral cortex (outside the medial temporal lobes), sometimes involving cerebellum and brainstem; transient enhancement of overlying meninges Sometimes: normal or medial temporal and frontal abnormalities, with or without basal FLAIR or T2 involvement of other areas

Abbreviation: FLAIR, fluid-attenuated inversion recovery.

NMDAR antibodies are present in serum and CSF, usually with intrathecal synthesis, and are sometimes only detected in the CSF. The main target epitopes are in the NR1/NR2 heteromers of the NMDAR. The major antigen is NR1/NR2B, which is predominantly expressed in the hippocampus and forebrain, but reactivity with other NR1/NR2 heteromers is frequently observed.2 In the present case, the patient had serum and CSF antibodies that reacted with all NR1/NR2 heteromers (NR2A–NR2D).

The associated tumors are mature (dermoid cyst) or immature teratomas of the ovary; recurrent or bilateral tumors can occur.6 A CT scan and ultrasound of the pelvis, or intravaginal ultrasound, usually reveals the tumor. As 70% of the tumors are benign, PET scans can be negative.1 In one patient the teratoma was in the mediastinum.2 In all patients studied the NMDAR antibodies reacted with nervous tissue contained in the tumor (J Dalmau, unpublished data).2


The current patient presented with low-grade fevers, headaches and anxiety that progressed to paranoia, homicidal ideation, and seizures. These symptoms suggested several toxic and metabolic disorders that were excluded by the patient’s history, and by blood and urine testing.

The disorders most frequently considered in the differential diagnosis of 21 patients with anti-NMDAR encephalitis are shown in Table 1 (J Dalmau, unpublished data).2 In the present case, the CSF inflammatory abnormalities and MRI FLAIR hyperintensity in the patient’s temporal lobes were consistent with viral or autoimmune encephalitis. HSV encephalitis was suspected, but several HSV PCRs of CSF were negative, and there was no response to aciclovir.7,8 Other viral encephalitides were excluded with appropriate testing.

Table 1
Disorders frequently considered in the differential diagnosis of N-methyl-D-aspartate receptor antibody-associated encephalitis.a

Most autoimmune encephalitides cause psychiatric symptoms and CSF inflammatory abnormalities; however, MRI scans revealing hyperintensities involving medial temporal lobes are frequent in only two disorders: the paraneoplastic limbic encephalitides,9 and encephalitis associated with voltage-gated potassium channel antibodies.10 These disorders (Table 1) are usually associated with characteristic antibodies that were not present in the current patient.

Antibodies to NR2 subunits of the NMDAR have been reported in several other disorders.11,12 These antibodies (IgG or IgM) target linear epitopes (detectable by immunoblot), often in the intracellular domain. By contrast, the IgG antibodies of patients with paraneoplastic anti-NMDAR encephalitis target conformal extracellular epitopes of NR1/NR2 heteromers (not detectable by immunoblot), and are likely to be pathogenic.

The triad of alteration in mental status, rigidity, and dysautonomia can occur in the neuroleptic malignant syndrome (NMS), serotonin syndrome, or lethal catatonia. Although mechanistically distinct, these disorders can all include muscle hyperactivity, hyperthermia, metabolic acidosis, rhabdomyolysis, elevated creatine kinase (CK), seizures and renal failure.13 NMS and serotonin syndrome can be confused with anti-NMDAR encephalitis as a result of the fact that most patients with the latter are initially seen in psychiatric centers and might receive neuroleptics or antidepressants.14 Once hospitalized, the present patient was not given serotonergic medications and her serum muscle enzymes were normal. Moderate elevation of CK occurred in 1 of 21 patients with paraneoplastic anti-NMDAR encephalitis (J Dalmau, unpublished data). Most of these patients, however, had inflammatory CSF findings not found in NMS, serotonin syndrome, or lethal catatonia. One patient with paraneoplastic catatonia and ovarian teratoma had elevated CK levels; she partially improved with electroconvulsive therapy, but full recovery was only attained after tumor removal (serum and CSF were not available for antibody analysis).15


A study of 12 patients indicated that the optimal management of anti-NMDAR encephalitis is tumor resection and immunotherapy.2 Patients with this disorder, however, usually require ventilatory support and intensive care for seizures and autonomic instability, which can delay tumor removal. Treatment with methylprednisolone, plasma exchange or IVIg might result in partial neurological improvement or stabilization allowing resection, although the tumor could grow during the delay.3 In some patients, tumor removal results in noticeable neurological improvement in a matter of days or after several weeks. The present patient was diagnosed the day after admission and the tumor was resected that evening. Despite this rapid intervention, she did not start to improve until 3 weeks later, after plasma exchange, IVIg, and a bolus of cyclophosphamide. The latter was used because of previous experience with other patients who did not improve within 1 week of completing plasma exchange or IVIg. Once improvement is noted, most patients continue to improve over weeks or months until full recovery. During improvement, no maintenance immunotherapy is needed. As a result of general deconditioning, and sometimes spinal cord involvement, patients often need prolonged physical therapy.2,16


Paraneoplastic anti-NMDA receptor encephalitis is potentially lethal, but usually reversible if promptly recognized and treated. It should be suspected in young women with prominent psychiatric symptoms accompanied by seizures, autonomic instability, hypoventilation and dyskinesias. Less frequently it presents as classical limbic encephalitis. The CSF usually shows inflammatory abnormalities, and the MRI can be normal, show transient cerebral cortical or cerebellar abnormalities, or show typical medial temporal lobe hyperintensities. The associated tumors are mature or immature teratomas, usually located in the ovary. Preliminary experience suggests that tumor resection, immunomodulation, intensive care, and physical therapy constitute the best treatment approach.


We thank Dr Myrna R Rosenfeld for critical review of the manuscript. This work was supported in part by RO1CA107192 (JD) and NS45986 (DL) from the National Institute of Health (NIH).


Vanderbilt Continuing Medical Education online This article offers the opportunity to earn one Category 1 credit toward the AMA Physician’s Recognition Award.

Competing Interests The authors declared they have no competing interests.


1. Vitaliani R, et al. Paraneoplastic encephalitis, psychiatric symptoms, and hypoventilation in ovarian teratoma. Ann Neurol. 2005;58:594–604. [PMC free article] [PubMed]
2. Dalmau J, et al. Paraneoplastic anti-N-methyl-D-aspartate receptor encephalitis associated with ovarian teratoma. Ann Neurol. 2007;61:25–36. [PMC free article] [PubMed]
3. Shimazaki H, et al. Reversible limbic encephalitis with antibodies against membranes of neurons of hippocampus. J Neurol Neurosurg Psychiatry. 2007;78:324–325. [PMC free article] [PubMed]
4. Stein-Wexler R, et al. Paraneoplastic limbic encephalitis in a teenage girl with an immature ovarian teratoma. Pediatr Radiol. 2005;35:694–697. [PubMed]
5. Koide R, et al. EFA6A-like antibodies in paraneoplastic encephalitis associated with immature ovarian teratoma: a case report. J Neurooncol. 2007;81:71–74. [PubMed]
6. Muni RH, et al. Bilateral horizontal gaze palsy in presumed paraneoplastic brainstem encephalitis associated with a benign ovarian teratoma. J Neuroophthalmol. 2004;24:114–118. [PubMed]
7. Tyler KL. Update on herpes simplex encephalitis. Rev Neurol Dis. 2004;1:169–178. [PubMed]
8. Wainwright MS, et al. Human herpesvirus 6 limbic encephalitis after stem cell transplantation. Ann Neurol. 2001;50:612–619. [PubMed]
9. Gultekin SH, et al. Paraneoplastic limbic encephalitis: neurological symptoms, immunological findings and tumour association in 50 patients. Brain. 2000;123:1481–1494. [PubMed]
10. Vincent A, et al. Potassium channel antibody-associated encephalopathy: a potentially immunotherapy-responsive form of limbic encephalitis. Brain. 2004;127:701–712. [PubMed]
11. Mochizuki Y, et al. Acute limbic encephalitis: a new entity? Neurosci Lett. 2006;394:5–8. [PubMed]
12. Takahashi Y, et al. Autoantibodies and cell-mediated autoimmunity to NMDA-type GluRepsilon2 in patients with Rasmussen’s encephalitis and chronic progressive epilepsia partialis continua. Epilepsia. 2005;46(Suppl 5):S152–S158. [PubMed]
13. Carbone JR. The neuroleptic malignant and serotonin syndromes. Emerg Med Clin North Am. 2000;18:317–325. [PubMed]
14. Boyer EW, Shannon M. The serotonin syndrome. N Engl J Med. 2005;352:1112–1120. [PubMed]
15. Lee A, et al. Electroconvulsive therapy in a pediatric patient with malignant catatonia and paraneoplastic limbic encephalitis. J ECT. 2006;22:267–270. [PubMed]
16. Taylor RB, et al. Reversible paraneoplastic encephalomyelitis associated with a benign ovarian teratoma. Can J Neurol Sci. 1999;26:317–320. [PubMed]