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The inhibition of apoptosis of infected host cells is a well-known but poorly understood function of pathogenic mycobacteria. We show that inactivation of the secA2 gene in Mycobacterium tuberculosis, which encodes a component of a virulence-associated protein secretion system, enhanced the apoptosis of infected macrophages by diminishing secretion of mycobacterial superoxide dismutase. Deletion of secA2 markedly increased priming of antigen-specific CD8+ T cells in vivo, and vaccination of mice and guinea pigs with a secA2 mutant significantly increased resistance to M. tuberculosis challenge compared with standard M. bovis bacille Calmette-Guérin vaccination. Our results define a mechanism for a key immune evasion strategy of M. tuberculosis and provide what we believe to be a novel approach for improving mycobacterial vaccines.
Mycobacterium tuberculosis infects more than one-third of the world’s population and causes 2–3 million deaths annually (1).The only currently approved vaccine for tuberculosis (TB) is bacille Calmette-Guérin (BCG), which is an attenuated strain of M. bovis. This vaccine has been widely administered in TB-endemic regions, with little apparent impact on the prevalence of adult pulmonary TB, the most common form of disease caused by M. tuberculosis (2). Given the difficulties inherent in antibiotic treatment of TB (i.e., cost and long duration of continuous therapy), the increasing prevalence of active disease due to HIV coinfection, and the emergence of multidrug resistance, there is an urgent need for the development of new and more effective candidate vaccines (3).
Effective host control of M. tuberculosis is critically dependent on both innate and adaptive immune responses, and detailed studies in mouse models show that priming of both CD4+ and CD8+ T cells is important for long-term control of latent infection and for vaccine-induced protection (4). However, many lines of evidence indicate that M. tuberculosis has evolved mechanisms to evade host immunity, in some cases by inhibiting the priming and effector functions of these T cell subsets (5). One property of M. tuberculosis that has been linked to evasion of host immune responses is its ability to prevent the death by apoptosis that is typically triggered upon infection of phagocytic cells by vacuolar bacterial pathogens (6–8). Whereas macrophages infected by nonpathogenic species of mycobacteria are observed to rapidly undergo apoptosis, virulent mycobacteria such as M. tuberculosis and M. bovis (including BCG) do not efficiently trigger this response (9–12). In vitro studies indicate that the death of infected cells by apoptosis contributes to clearance of the bacteria through activation of as-yet-undefined bactericidal mechanisms (10, 11). In addition, apoptosis of infected cells has been shown to facilitate the transfer of mycobacterial antigens for presentation to T cells through MHC class I and CD1 molecules, which has been designated the “detour pathway” for antigen cross-presentation (13–15). Thus, the inhibition of the apoptotic response of infected host cells by M. tuberculosis is likely to interfere with both innate and adaptive immunity against this pathogen and most likely represents an important immune evasion strategy of this bacterium.
Although previous studies clearly document the antiapoptotic properties of M. tuberculosis, little is known regarding the mechanism of this immune evasion strategy, nor have any specific genes of M. tuberculosis controlling this aspect of virulence been reported. Here we show that the secA2 gene of M. tuberculosis, which encodes a component of a virulence-associated bacterial protein secretion system, is required for prevention of apoptosis of M. tuberculosis–infected macrophages. Our analyses showed that this was attributable to SecA2-dependent secretion of mycobacterial superoxide dismutase A (SodA). Infection with a secA2 deletion mutant of M. tuberculosis not only led to markedly increased levels of apoptosis by infected macrophages, but was also associated with significant enhancement in the priming of naive antigen-specific CD8+ T cells in vivo, suggesting increased engagement of the apoptosis-dependent detour pathway for MHC class I–restricted cross presentation. Vaccination of mice or guinea pigs with the secA2-deleted strain generated a level of protective host immunity against challenge with virulent M. tuberculosis that was significantly greater according to several criteria than the protection induced by BCG vaccination. These findings suggest a novel and practical approach to improving live TB vaccine strains by genetic modifications that disrupt their ability to interfere with host cell apoptosis.
The secA2 gene (Rv1821) encodes an accessory secretion factor in M. tuberculosis that is required for the export of a small subset of proteins that includes SodA, an M. tuberculosis enzyme that catalyzes conversion of superoxide anions to hydrogen peroxide (16). Whereas deletion of secA2 from M. tuberculosis has no effect on growth in culture, it does result in partial growth attenuation in vivo. This may be explained in part by the loss of SodA secretion, which would be expected to result in a reduced ability of the intracellular bacteria to detoxify superoxide anions generated in host cells in response to infection (17). However, overproduction of superoxide anions is also linked to induction of apoptosis in a variety of experimental systems (18), which suggested that another important function of secreted mycobacterial SodA may be to inhibit the infection-induced apoptosis of host cells. Indeed, when compared with nonpathogenic species of mycobacteria that are known to be proapoptotic, pathogenic mycobacteria such as M. tuberculosis express markedly higher levels of SodA (19), and antisense inhibition of SodA expression in M. tuberculosis results in reduced bacterial growth and higher levels of apoptosis in tissues of infected mice (20).
To directly examine a role for secA2 in the inhibition of apoptosis, transformed human monocyte/macrophage cells (THP-1 cells) or primary mouse BM-derived macrophages (BMMs) (C57BL/6 or BALB/c strains) were infected at an MOI of 10 bacilli per macrophage with WT M. tuberculosis (strain H37Rv), a mutant of H37Rv with a secA2 deletion (ΔsecA2), or a complemented strain of the mutant (ΔsecA2-C). After 72 hours, quantitative assessment of apoptosis by staining for DNA strand breakage (TUNEL) and polycaspase activation showed that ΔsecA2 strongly induced both of these markers of apoptosis in both human and murine macrophages, whereas WT M. tuberculosis did not (Figure (Figure1,1, A and B, and Supplemental Figure 1; supplemental material available online with this article; doi:10.1172/JCI31947DS1). The enhanced TUNEL staining was fully reversed with ΔsecA2-C, which behaved identically to the WT strain (Figure (Figure1,1, A and B).
Caspases are known to be activated by infection with virulent as well as nonvirulent strains of M. tuberculosis (10, 21), although under certain conditions, induction of macrophage death by M. tuberculosis has been reported to proceed in a caspase-independent manner (22). The involvement of caspases in apoptosis induced by ΔsecA2 M. tuberculosis was evaluated by measuring total caspase activity and activities of caspases 8 and 9 at 48 and 72 hours after infection in THP-1 cells (Figure (Figure1A,1A, and data not shown). Caspases 8 and 9 were selected because they are known to be initiators of the extrinsic (receptor-mediated) and intrinsic (mitochondrial) cell death pathways, respectively (23). The 2 caspases were equally activated in cells infected with ΔsecA2 but not in cells infected with either WT M. tuberculosis or the complemented ΔsecA2-C. This suggested the involvement of both extrinsic and intrinsic caspase-dependent pathways for initiation of apoptosis by ΔsecA2. Furthermore, treatment of THP-1 cells with an irreversible specific inhibitor of caspase-3 prior to infection with the ΔsecA2 mutant strongly reduced TUNEL staining, indicating that the macrophage cell death induced by ΔsecA2 was dependent on the activation of this downstream caspase (Figure (Figure1C). 1C).
In contrast to mycobacterial proteins that undergo signal peptide–dependent secretion by the secA1-dependent pathway, SodA does not possess a recognizable N-terminal signal sequence. We therefore reasoned that extending the N terminus of SodA with a signal sequence from a mycobacterial protein secreted by a secA2-independent pathway might restore SodA secretion in the ΔsecA2 mutant and reverse its apoptosis-inducing phenotype. This was tested by extending the 5′ end of the coding sequence for WT SodA with a sequence encoding the N-terminal signal sequence of Ag85b, a major M. tuberculosis protein that is secreted in a secA1-dependent and secA2-independent manner (16). Expression of this construct in the ΔsecA2 mutant (ΔsecA2-αsodA) restored SodA activity in the culture filtrate and also reversed the induction of apoptosis in infected THP-1 cells (Figure (Figure1D).1D). This result indicated that secretion of SodA was likely to be the major secA2-dependent process involved in the inhibition of host cell apoptosis.
Studies of Salmonella typhimurium and other vacuolar bacteria show that death of infected cells by apoptosis leads to efficient uptake of bacterial antigens by host DCs and cross-presentation to MHC class I–restricted CD8+ T cells (8). Because M. tuberculosis antigens are generated within a phagosomal compartment that is sequestered from the classical cytoplasmic pathway of antigen processing, display of these antigens on MHC class I molecules is likely to depend on an indirect route of presentation through bystander DCs, which can take up and process material released from apoptotic infected macrophages via the previously described detour pathway for cross-presentation (13–15). Based on our observation of enhanced apoptosis by the ΔsecA2 mutant in vitro, we predicted that this mutant should also facilitate transmission of antigens to bystander DCs and enhance CD8+ T cell priming. To test this in vivo, we expressed a plasmid encoding the M. tuberculosis 19-kDa lipoprotein fused at its C terminus to the H-2Kb–binding sequence of OVA (SIINFEKL) in WT M. tuberculosis (H37Rv-OVA), an attenuated mutant of M. tuberculosis with a deletion of the RD1 region (24) (ΔRD1-OVA), or the ΔsecA2 mutant (ΔsecA2-OVA) (Supplemental Figure 2). Mice were infected with clones of these recombinant mycobacteria expressing comparable levels of the 19-kDa lipoprotein–SIINFEKL fusion protein (Supplemental Figure 2A), and SIINFEKL-responsive CD8+ T cells in their spleens were enumerated by IFN-γ ELISPOT. Whereas infection with H37Rv, H37Rv-OVA, or ΔRD1-OVA induced only background levels of IFN-γ spot–forming cells, the ΔsecA2-OVA infection induced a significant, 10-fold increase above background levels (Figure (Figure2A).2A). As another important gauge of CD8+ T cell priming, we assessed SIINFEKL-specific cytolytic activity using an in vivo CTL assay. This revealed weak cytolytic activity in mice infected with H37Rv-OVA but a substantial increase in target-specific killing in mice infected with ΔsecA2-OVA (Figure (Figure2B).2B). In contrast to the clear enhancement of T cell responses against an MHC class I–presented epitope in animals primed with the ΔsecA2-OVA strain, IFN-γ ELISPOT responses to crude exogenous mycobacterial proteins contained in M. tuberculosis purified protein derivative (PPD) or the dominant MHC class II–presented epitope of Ag85B (Pep25) were similar in animals infected with WT and ΔsecA2 M. tuberculosis (Figure (Figure2C).2C). This indicated that enhancement of T cell priming by the ΔsecA2 mutant was limited mainly or exclusively to the CD8+ T cell compartment.
To directly visualize the priming of MHC class I–restricted CD8+ T cells reactive with SIINFEKL in the context of infection, we used adoptive transfer of CFSE-labeled naive T cells from SIINFEKL/H-2Kb–reactive TCR-transgenic OT-I mice (25). Thy1.1+ B6.PL mice were injected with CFSE-labeled Thy1.2+ splenocytes from OT-I mice, followed by infection with mycobacteria expressing the 19-kDa lipoprotein–SIINFEKL fusion protein. CD8+ T cell activation and proliferation were assessed by dilution of CFSE in the transferred population at 5–7 days after infection (Figure (Figure2D).2D). Negligible proliferation of transferred OT-I T cells was observed in mice infected with H37Rv, and very modest proliferation was seen with H37Rv-OVA infection. In contrast, ΔsecA2-OVA infection induced a massive increase in proliferation of transferred T cells, with the vast majority of cells undergoing 8 or more divisions. The response to ΔsecA2-OVA was most marked in mice that had been adoptively transferred with relatively small numbers (3 × 105) of OT-I T cells, consistent with previous observations on the effect of precursor frequency on limiting CD8+ T cell expansion (26). Restoration of secA2 by complementation (ΔsecA2-C-OVA) reversed the effect of secA2 deletion on OT-I T cell priming (Figure (Figure2D).2D). In contrast to the marked differences in OT-I T cell proliferation to infection by secA2-negative versus -positive strains, CD8+ T cell responses to lysates of the same strains injected subcutaneously with an adjuvant known to stimulate cross-priming (QS-21) were similar (Supplemental Figure 2C). This confirmed that the SIINFEKL epitope was produced in comparable amounts and in a potentially immunogenic form by all of the different recombinant mycobacterial clones, although efficient presentation of antigen originating from live mycobacteria to OT-I T cells occurred only during infection by ΔsecA2 bacilli.
Since selective reconstitution of secreted SodA activity by expression of αsodA completely reversed apoptosis induction by ΔsecA2 mutants (Figure (Figure1E),1E), we assessed the effect of this on the priming and expansion of OT-I T cells by generating a ΔsecA2-αsodA strain expressing the 19-kDa lipoprotein–SIINFEKL fusion protein (ΔsecA2-αsodA-OVA). Infection with ΔsecA2-αsodA-OVA resulted in markedly reduced expansion of adoptively transferred OT-I T cells compared with ΔsecA2-OVA and was similar in this regard to WT H37Rv-OVA infection (Figure (Figure2E).2E). This indicated a tight linkage among enhanced CD8+ T cell priming, the loss of SodA secretion, and the inability of the bacterium to block apoptosis of its infected host cell.
An important benchmark for measuring the efficacy of a vaccine strain is the effect it has on the generation of long-lived memory T cells. Because FACS analysis of endogenous OVA-specific T cells using SIINFEKL-loaded H-2Kb tetramers was not sufficiently sensitive for accurate quantitation (data not shown), we used adoptive transfer of low numbers (5 × 105) of OT-I T cells to boost the precursor frequency of OVA-specific T cells. This enabled accurate quantitation of antigen-specific CD8+ T cells by tetramer staining in mice vaccinated by subcutaneous injection with H37Rv-OVA or ΔsecA2-OVA. By following levels of SIINFEKL-loaded H-2Kb tetramer+ splenocytes over a period of 20 weeks, we observed that ΔsecA2-OVA vaccinated mice showed an enhanced expansion phase of antigen-specific CD8+ T cells that was followed by a sustained maintenance phase characterized by increased levels of CD8+ memory T cells (Figure (Figure3).3). We also measured expression of CD62 ligand (CD62L) and CD44 on the tetramer+ cells to distinguish among naive (CD44lowCD62Lhigh), central memory (CD44highCD62Lhigh), and effector memory (CD44highCD62Llow) subsets (27). An increase in the long-term expansion of CD8+ memory T cells to at least 8 weeks after vaccination with ΔsecA2 was apparent in both the CD62Lhigh and CD62Llow subsets, with a trend favoring greater and more persistent expansion in the CD62Lhigh population. We also observed higher expression of IL-7 receptor α chain and lower expression of the programmed death–1 receptor (PD-1) in expanded antigen-specific CD8+ T cells in ΔsecA2-vaccinated mice at 20 weeks (data not shown), consistent with the generation of a long-lived and functionally intact population of memory T cells (28, 29).
In order to determine whether the enhanced T cell priming observed with ΔsecA2 could increase its efficacy as a live vaccine strain, we conducted immunization and challenge studies designed to evaluate potential vaccine efficacy. C57BL/6 mice that were either naive or immunized 8 weeks earlier by subcutaneous inoculation with live ΔsecA2 or BCG (Pasteur strain) were challenged by low-dose aerosol infection with virulent M. tuberculosis (strain Beijing W/HN878). In naive mice, substantial growth in the lungs and dissemination to spleens were detected within 1 month of challenge. In contrast, vaccination with ΔsecA2 and BCG considerably reduced M. tuberculosis bacillary loads in both lungs and spleens of aerosol-challenged mice as compared with naive controls at 1 month after aerosol challenge (Figure (Figure4A).4A). The protection evoked by ΔsecA2 immunization was significantly greater than that obtained with BCG vaccination, as measured by bacterial burden in lung (0.72-log reduction in ΔsecA2 versus BCG vaccinated mice; P < 0.001). Immunization with ΔsecA2 also showed a more prolonged effect on control of M. tuberculosis infection compared with BCG immunization, with reductions in CFU still apparent 5 months after challenge, when BCG immunization no longer showed significant effects. Moreover, the survival of M. tuberculosis–challenged mice vaccinated with ΔsecA2 was significantly prolonged when compared with that of naive mice or mice vaccinated with BCG (Figure (Figure4C).4C). Histopathological examination of the lungs from mice immunized with either BCG or the ΔsecA2 strain showed relatively mild inflammation with small and compact lymphocyte-rich granulomas, compared with naive mice, which had extensive, poorly organized granulomatous lesions (Figure (Figure4B). 4B).
We also tested the protective capacity of ΔsecA2 vaccination using an aerosol challenge model in guinea pigs, a host species highly susceptible to M. tuberculosis. Animals that were either naive or immunized 2 months previously with ΔsecA2 or BCG were challenged by low-dose aerosol inoculation of M. tuberculosis H37Rv. At 1 and 2 months after challenge, guinea pigs immunized with M. tuberculosis ΔsecA2 showed marked reductions in CFU in the spleens, lungs, and mediastinal lymph nodes relative to naive controls. Although BCG-immunized animals also showed similar reductions in CFU in the lung and spleen, these animals showed much less reduction of bacillary load in the mediastinal lymph nodes than was seen for the ΔsecA2-immunized group (Figure (Figure5A).5A). The results of pathologic evaluation of guinea pig tissues paralleled the bacterial counts in the tissues (Figure (Figure5B;5B; Supplemental Figure 4). Again, the differences in the mediastinal lymph nodes were striking, as these were markedly enlarged in the naive and BCG-immunized groups but appeared normal in size and gross appearance in ΔsecA2-immunized animals. Microscopically, the lymph nodes of both naive and BCG-immunized animals showed marked granulomatous reaction progressing to fibrosis that completely effaced the normal architecture of the nodes by 2 months, while the lymph nodes of ΔsecA2-immunized animals appeared normal at 1 month and showed only small areas of fibrosis at 2 months after challenge. Taken together, these results in 2 different animal models of M. tuberculosis vaccination and challenge indicated that ΔsecA2 was at least equal to BCG in all outcomes examined and significantly exceeded BCG in some measures of efficacy such as prolongation of survival and the ability to restrict extension of pulmonary infection into the regional lymphatic system.
Our results confirm a significant role for inhibition of infected host cell apoptosis in the immune evasion strategy of M. tuberculosis and suggest the secA2-dependent secretion of mycobacterial SodA as a key mechanism involved in this virulence-associated trait. In this regard, virulent mycobacteria appear to have adopted an evasion strategy that is characteristic of many viruses that also produce factors that can delay apoptotic host cell death (30). Whereas the ability of virulent mycobacteria to perform this function has been previously described (9–11, 22), the work reported here is to our knowledge the first demonstration of a specific genetic deletion in M. tuberculosis that curtails the ability of this organism to inhibit apoptosis of infected macrophages. Although our results showed that secA2 was essential for the antiapoptotic properties of M. tuberculosis, it is likely that other mechanisms also cooperate with the secA2-dependent mechanism described here in blocking all of the multiple pathways that can initiate apoptosis. Indeed, our preliminary studies have identified at least 3 additional genes in M. tuberculosis that are required for effective blockade of infected macrophage apoptosis (unpublished observations), although it is currently unclear which pathways for induction or execution of programmed cell death these are inhibiting.
Several advantages may accrue to mycobacteria as a result of delaying or inhibiting macrophage apoptosis, including preservation of a protected growth environment and prevention of engagement of intrinsic microbicidal activities in the host cell (31, 32). The studies presented here also underscore the key role of blockade of apoptosis in preventing optimal stimulation of protective immunity. This effect was particularly evident in our studies of CD8+ T cell priming and memory T cell generation, in which WT M. tuberculosis showed remarkably poor immunogenicity when compared with the ΔsecA2 mutant. CD8+ T cells have been found by several criteria to be a significant component of antimycobacterial immunity in mouse models (33–36), and they can potentially contribute to containment or eradication of M. tuberculosis via several mechanisms. These include the secretion of protective cytokines such as IFN-γ and TNF, cytolytic activity against infected macrophages, and even direct bactericidal effects on intracellular mycobacteria (4, 37, 38). Although CD4+ T cell responses were not measurably enhanced by deletion of secA2 in our experiments (Figure (Figure2C),2C), we have observed that vaccination with M. tuberculosis strains including ΔsecA2 generally prime stronger CD4+ T cell responses when compared directly with BCG (data not shown). This raises the possibility that the enhanced protection by ΔsecA2 M. tuberculosis compared with BCG observed in the current study could be due at least in part to an augmented CD4+ T cell response. However, in our experience so far, the ΔsecA2 strain is unique among M. tuberculosis mutants in its ability to provide a higher level of protection than BCG in animal challenge models (ref. 39 and unpublished observations). This strongly suggests that the apoptosis-promoting and CD8+ T cell–augmenting properties of ΔsecA2 M. tuberculosis are likely to be important to its ability to generate significantly increased levels of protective immunity. Further studies will be necessary to determine whether the strong protective effects of immunization with the ΔsecA2 strain can be completely attributed to the enhanced CD8+ T cell response or whether modulation of other key components of the adaptive response is also involved.
Interestingly, it has recently been shown that ΔsecA2 M. tuberculosis exhibits attenuated growth in isolated mouse macrophages in vitro and that this phenotype is observed even in macrophages that cannot generate high levels of reactive oxygen intermediates because of inactivation of the inducible phagosome oxidase complex (40). This finding is consistent with earlier studies showing that superoxide and other reactive oxygen intermediates contribute little to the antimycobacterial activity of macrophages (41). While this does not contradict our findings on the effects of secA2 deletion on macrophage survival and T cell priming, it does suggest that mycobacterial secretion of SodA is more directly protective of the host cell than of the mycobacterium itself and that the perturbation of other secreted effectors may be responsible for the attenuated growth of ΔsecA2 M. tuberculosis in macrophages.
Previous work by Kaufmann and colleagues has demonstrated that modifying BCG to express listeriolysin improves its ability to stimulate protective immunity in mice, and this may also be associated with an increased ability to induce apoptosis of macrophages (42). Our demonstration of the enhancement of long-lived adaptive immunity by the M. tuberculosis ΔsecA2 mutant provides a rational and straightforward basis for improving the immunogenicity of live attenuated mycobacterial strains for use as M. tuberculosis vaccines (43). By combining ΔsecA2 with additional mutations that disable various other immune evasion– and virulence-promoting functions of M. tuberculosis, it may be possible to develop a new generation of safe and effective attenuated mycobacterial vaccine strains that will have greater impact than BCG on the current global TB problem.
Virulent M. tuberculosis strains H37Rv (obtained from Trudeau Institute), Erdman, HN878 (obtained from C. Barry, Tuberculosis Research Section, NIH, Bethesda, Maryland, USA), and M. tuberculosis ΔsecA2 mutant (mc23112) have been described previously (16, 44). Virulent M. tuberculosis strains, mutants, and transformants were grown on Middlebrook 7H9 broth or 7H10 agar containing 10% (vol/vol) oleic acid–albumin-dextrose-catalase (OADC; BD Diagnostics — Diagnostic Systems), 0.5% glycerol, and 0.05% (vol/vol) Tween-80. The E. coli strain DH5α was used for cloning purposes and grown on Luria-Bertani agar or broth (Fisher Scientific) at 37°C. Ampicillin (50 μg/ml), hygromycin (50 μg/ml for mycobacterial selection or 150 μg/ml for E. coli), and kanamycin (20 μg/ml for mycobacterial selection or 40 μg/ml for E. coli) were used to select for recombinant strains.
For complementation of the secA2 deletion, a full-length M. tuberculosis secA2 gene was amplified by PCR from M. tuberculosis H37Rv genomic DNA using primers SecC5 (5′-AAGCTTGCGTGAACGTGCACGGTTGTCCACGAATTGC-3′) and SecC3 (5′-ATCGATTCAGCGGAACACCCCGGGCAGACT-3′). The PCR product was cloned into the pGEM vector using a TA cloning kit (Invitrogen). The fragment containing the secA2 gene was excised and cloned into pMV361, an integrating M. tuberculosis vector with an HSP60 promoter (45). All transformations of M. tuberculosis strains were performed according to a published protocol (46).
For epitope tagging of the 19-kDa lipoprotein, an extended primer approach was used. The 19-kDa lipoprotein gene was amplified from M. tuberculosis H37Rv genomic DNA using a primer directed at the 5′ end of the coding sequence for the 19-kDa lipoprotein gene (5′-GATATCGTGAAGCGTGGACTGAC-3′) in combination with a 3′ primer extended to contain a sequence from OVA encoding amino acids 254–269 (5′-GCTAGCATTTAAAACGAGGTCCACTCGGTCAGCTTCTCGAAGTTGATGATCGACTCCAGCTGCTCCAGACAGGTCACCTCGATTTCGAACGACTTG-3′). The PCR product containing the 19-kDa–OVA sequence was ligated into pMV261, a replicating extrachromosomal M. tuberculosis vector with an HSP60 promoter (45). The resulting plasmid, designated p19OVA, was used to transform WT, mutant, or complemented strains of M. tuberculosis H37Rv.
To generate a form of the SodA protein with a signal sequence recognized by the SecA1 secretion system, the sequences encoding the signal peptide of antigen 85b and the entire sodA gene were amplified from M. tuberculosis H37Rv genomic DNA using the primer pairs 5′-GATATCATGGCCACAGACGTGAGCCGAAAGATTCGAG-3′ plus 5′-GCCGGGATCCCGCGCCCGCGGTTGCCGCTCCGCC-3′ and 5′-GCTAGCTCAGCCGAATATCAACC-3′ plus 5′-GCTAGCTCAGCCGAATATAACC-3′, respectively. Each PCR product was separately cloned into the pGEM vector using a TA cloning kit (Invitrogen). In a stepwise fashion, these fragments were excised and ligated into pMV361 to create pMV3α-sod.
H37Rv, H37Rv-OVA, ΔsecA2-OVA, and ΔsecA2-C-OVA were grown to mid-log phase in 7H9 medium. After centrifugation, pellets were washed twice with PBS and lysed with protein extraction buffer (50 mM Tris-HCl, 5 mM EDTA, 0.6% SDS, 0.06% DNase, 0.06% RNase, 0.07% pepstatin, 0.05% leupeptin, and 20 mM PMSF). The protein concentration of each lysate was determined by BCA assay (Pierce). Equal amounts of protein (20 μg) were loaded per lane and subjected to SDS-PAGE followed by transfer to a PVDF membrane. After blocking with PBS plus 5% skim milk, the membrane was incubated with a rabbit polyclonal anti-SIINFEKL antibody (Covance), followed by detection with a goat anti-rabbit antibody linked to HRP (Invitrogen). Specific bands were illuminated using SuperSignal West Pico stable peroxide solution (Pierce).
BMMs were derived according to previously published methods (5). Briefly, marrow flushed from the tibia and femurs of 6- to 10-week-old C57BL/6 or BALB/c mice (The Jackson Laboratory) was aseptically collected and cultured in non–tissue culture–treated 100 × 20–mm serological plates (Fisher) at a density of 2 × 105 cells/ml in BMM medium (DMEM supplemented with 1% HEPES, 1% l-glutamine, 1% nonessential amino acids, 100 U/ml penicillin, and 100 μg/ml streptomycin [all from Gibco BRL; Invitrogen], 10% FBS [HyClone], and 10% L929 fibroblast-conditioned medium) for 6 days. On day 6, adherent cells were harvested after detachment by incubation for 20 minutes at 4°C with 5 mM EDTA/PBS.
THP-1 cells (ATCC) were grown in RPMI-1640 (Gibco BRL; Invitrogen) supplemented with 10% FBS, 1% HEPES, 1% nonessential amino acids and essential amino acids, and 50 μg/ml of penicillin and streptomycin (complete RPMI). THP-1 cells were treated with 40 ng/ml PMA (Sigma-Aldrich) overnight to induce their differentiation into macrophage-like cells.
BMMs or differentiated THP-1 cells were plated in 6-well plates at 1 × 106 cells per well and allowed to adhere overnight. For C57BL/6 macrophages, pretreatment with 25 ng/ml IFN-γ (PeproTech) for 24 hours before infection was used to activate the cells and increase their sensitivity to apoptosis induction. Dispersed bacilli were incubated with cells for 3 hours (MOI of 10). The cells were then washed twice with PBS and incubated for 2–4 days as indicated in the figure legends. After infection, BALB/c BMMs were maintained in BMM medium (with 10% L929 conditioned medium, which was necessary to prevent high background levels of spontaneous cell death), while C57BL/6 BMMs were maintained in BMM medium without L929 conditioned medium. The presence of apoptotic cells in the cell cultures was analyzed with In Situ Cell Death Detection Kit, Fluorescence (Roche Diagnostics) and Carboxyfluorescein FLICA Apoptosis Detection Kit Caspase Assay (Immunochemistry Technologies), following the manufacturer’s instructions. For caspase-3 inhibition assays, THP-1 cells were treated for 1 hour before infection with the irreversible caspase-3 inhibitor Z-DEVD-FMK (R&D Systems) at a final concentration of 10 μM, then washed and infected with mycobacteria as indicated.
Equal numbers of H37Rv, ΔsecA2, ΔsecA2-C, and ΔsecA2-αsodA bacilli were first inoculated into a 10-ml culture of Sauton liquid medium (16) and then grown at 37°C for 7 days. Five milliliters of the initial culture was then inoculated into 50 ml of Sauton medium and grown for 7 days. Cultures were centrifuged, and supernatants collected and filtered through a 0.2-μm filter and then concentrated 50-fold using a Centriprep centrifugal filter with a 3-kDa molecular weight limit (Millipore). Protein concentration was determined by BCA assay (Pierce). SodA activity was measured using SOD determination kit-WST (Sigma-Aldrich), which uses a water-soluble tetrazolium salt, WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt] that produces a water-soluble formazan dye upon reduction by superoxide produced by xanthine oxidase. Superoxide dismutase (SOD) activity is determined by the inhibition of time-dependent formation of formazan dye, which is quantitated by absorbance at 405 nm. A volume of culture filtrate containing 3.5 μg of total protein was used for duplicate determinations of SOD activity for each bacterial culture filtrate, which gave a SOD activity inhibition rate of 10%–14% with WT H37Rv.
ELISPOT was used to detect IFN-γ secretion by individual CD8+ T cells from infected mice after stimulation with OVA peptide (SIINFEKL) in vitro. ELISPOT plates (Millipore) were coated with IFN-γ capture antibody (BD Biosciences) for 16 hours at room temperature (RT). Plates were washed and blocked with 1% BSA for 2 hours at RT. After treatment with rbc lysis buffer (Sigma-Aldrich), splenocytes were plated with OVA (5 μg/ml) peptide and cultured for 36 hours at 37°C. After cells were removed, plates were washed with PBS followed by PBS with 0.05% Tween-20 (PBST). Biotinylated anti–IFN-γ detection antibody (BD Biosciences) was added for 2 hours at 37°C, followed by washing with PBST. Streptavidin–alkaline phosphatase (Sigma-Aldrich) was added to the plates for 1 hour (37°C), followed by washing and addition of BCIP/NBT substrate (Sigma-Aldrich). After 2 hours of incubation at 37°C, the reaction was stopped by addition of water to the wells. Plates were fixed by addition of 4% paraformaldehyde, and spots were counted using an ELISPOT reader (Autoimmun Diagnostika). To evaluate CD4+ T cell responses, a similar procedure was used, except T cells were first separated using the Dynal Mouse T Cell Negative Isolation Kit (Invitrogen), and PPD (20 μg/ml; Statens Serum Institut) or peptide-25 (5 μg/ml; ref. 47) of M. tuberculosis Ag85B were used as recall antigens.
Splenocytes were isolated from naive C57BL/6 mice, treated with rbc lysis buffer, resuspended at 5 × 107/ml in PBS with 5% FBS, and labeled for 5 minutes at RT with CFSE (Invitrogen) at a concentration of 0.5 μM (CFSElow cells) or 10 μM (CFSEhigh cells). After washing twice with PBS, the CFSElow population was pulsed with 20 μM of the H-2Kb–binding OVA peptide (SIINFEKL) for 1 hour at 37°C in complete RPMI, then washed extensively with and resuspended in PBS. The 2 CFSE-labeled populations were mixed in equal proportions and injected i.v. (1 × 107 cells / mouse) into mycobacteria-infected (immune) or uninfected (naive) mice. After 16 hours, spleens were harvested, and the CFSElow and CFSEhigh lymphocyte populations were quantitated using a FACSCalibur flow cytometer (BD Biosciences). Data were analyzed using WinMDI software (version 2.8; http://facs.scripps.edu/software.html), and the percent of peptide-specific lysis was calculated using the following formula: % lysis = 100 – 100 × [(ratio of CFSElow to CFSEhigh for infected mice)/(ratio of CFSElow to CFSEhigh for uninfected mice)].
Donor splenocytes were isolated from Rag1 deficient OT-1 TCR-transgenic mice (Taconic/National Institute of Allergy and Infectious Diseases [NIAID]). After rbc lysis, cells were labeled with 20 μM CFSE for 5 minutes at RT at a concentration of 5 × 107 cells/ml in PBS plus 5% FBS. Cells were washed once with PBS plus 5% FBS and twice with PBS before injection into B6.PL (Thy1.1+) recipients (The Jackson Laboratory). Mice received either 3 × 105 or 1 × 107 labeled cells as indicated via the lateral tail vein and were then vaccinated subcutaneously or infected i.v. with 1 × 106 CFU of ΔsecA2-OVA or control bacterial strains. Splenocytes were harvested 5–7 days later, stained with anti-Thy1.2 and anti-B220 antibodies (both from BD Biosciences), and analyzed by flow cytometry. Expansion was quantified by gating on the transferred population (Thy1.2+) and measuring the percentage of undivided (CFSEhigh) cells within this population.
C57BL/6 mice were adoptively transferred with 5 × 105 OT-1 cells, infected subcutaneously 24 hours later with 1 × 106 CFU H37Rv-OVA or ΔsecA2-OVA, and sacrificed at 2, 4, or 20 weeks. Splenocytes were isolated, stained with PE-labeled peptide-loaded (SIINFEKL) H-2Kb tetramers in combination with antibodies specific to Thy1.2, CD44, CD62L, and B220 (all antibodies were purchased from BD Biosciences). Percentages of lymphocytes staining with tetramer and expressing markers consistent with central (CD44highCD62Lhigh) or effector (CD44highCD62Llow) memory cells were determined by flow cytometry. Peptide-loaded H-2Kb tetramers were obtained from the tetramer core facility at the Memorial Sloan-Kettering Cancer Center.
All animal studies were approved by the institutional animal care and use committees of the Albert Einstein College of Medicine, the Center for Biologics Evaluation and Research, and Colorado State University. C57BL/6 mice were vaccinated subcutaneously with 1 × 106 CFU of M. tuberculosis ΔsecA2 or M. bovis BCG-Pasteur as described previously (43). Aerogenic challenge was done 2 months later using a Glas-Col inhalation chamber to deliver 50–100 CFU per animal of M. tuberculosis Beijing/W (HN878) or Erdman strains. Mice were sacrificed at 1, 3, and 5 months after challenge. Lungs and spleens of individual mice were aseptically removed and homogenized separately in 5 ml normal saline plus 0.05% Tween-80 using a Seward Stomacher 80 blender (Tekmar). The homogenates were diluted serially and plated on Middlebrook 7H10 agar with hygromycin (50 μg/ml). Lung tissues were processed for histopathology using standard paraffin fixation, sectioning, and H&E staining. In the survival study, animals infected with M. tuberculosis Erdman were observed daily until they died or became moribund and were euthanized.
Outbred Hartley guinea pigs (Charles River Laboratories) were vaccinated intradermally with 1 × 103 M. tuberculosis ΔsecA2 or BCG-Pasteur, followed 6 weeks later by aerosol challenge with a low dose (10–30 CFU) of virulent M. tuberculosis H37Rv using a Madison chamber aerosol generation device (8). Lung, spleen, and lymph node tissues were harvested and processed for CFU determination and for histopathology at 1 and 2 months after challenge using methods similar to those described above for mice. The lymph nodes examined were a cluster located at the tracheal bifurcation that represents the tracheobronchial and cranial mediastinal nodes. For quantitative histopathological scoring of guinea pig tissue sections, sections were blindly arranged in a rank order from normal to most severe and then scored as follows: 0 = normal, 1 = mild, 2 = moderate, 3 = severe. The scoring was carried out by a veterinary pathologist and was based on the following factors: area of lung affected by inflammation; number and types of inflammatory cells; and levels of necrosis, mineralization, and fibrosis. The rank was then multiplied by the score for each section to obtain a rank score, after which the identity of the treatment groups was revealed and the mean and SD of the rank score for each group were calculated.
One-way ANOVA was used for analyses in which 3 or more experimental groups were compared. For analyses containing 6 or fewer groups, the Bonferroni post-hoc test was used to generate P values for selected pairwise comparisons. For analyses with greater than 6 groups, the Tukey post-hoc test was used. The log-rank test was used for comparison of survival curves. P values of less than 0.05 were considered significant. Prism 4.0 software (GraphPad) was used for all statistical analyses.
This work was supported by NIH/NIAID grants AI063537 (PO1 to S.A. Porcelli, W.R. Jacobs Jr., and J. Chan), AI54540 (to M. Braunstein), AI054697 (to I.M. Orme) and by the TB Vaccine Testing and Research Materials Contract (NIH/NIAID HHSN266200400091c) awarded to Colorado State University. The OT-1/Rag1 mouse was obtained from the NIAID exchange program. Flow cytometry studies were facilitated by the FACS Core Resources supported by the AECOM Center for AIDS Research (AI 051519) and the AECOM Cancer Center (CA 13330). S.A. Porcelli also acknowledges support from a Burroughs Wellcome Fund Award in Translational Research and a Career Scientist Award from the Hirschl Trust. We thank John Kim, Mei Chen, Weijun Liu, Amy Yang, Crystal Shanley, and Erin Smith for expert technical assistance with animal vaccine and challenge studies and Mi-Sun Koo and Tatjana Dragic for helpful discussions and critical reading of the manuscript.
Nonstandard abbreviations used: BCG, bacille Calmette-Guérin; BMM, BM-derived macrophage; CD62L, CD62 ligand; RT, room temperature; SodA, superoxide dismutase A; TB, tuberculosis.
Conflict of interest: The authors have declared that no conflict of interest exists.
Citation for this article: J. Clin. Invest. 117:2279–2288 (2007). doi:10.1172/JCI31947
Joseph Hinchey and Sunhee Lee contributed equally to this work.
Steven A. Porcelli and William R. Jacobs, Jr. contributed equally to this work.
See the related Commentary beginning on page 2092.