Restricted cell elongation in Arabidopsis hypocotyls is associated with a reduced average pectin esterification level

doi:10.1186/1471-2229-7-31

BMC Plant Biol. 2007; 7: 31.

Published online 2007 June 17. doi: 10.1186/1471-2229-7-31

PMCID: PMC1913053

Restricted cell elongation in Arabidopsis hypocotyls is associated with a reduced average pectin esterification level

Paul Derbyshire,¹ Maureen C McCann,² and Keith Roberts³

¹Department of Metabolic Biology, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

²Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA

³Department of Cell and Developmental Biology, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

Corresponding author.

Paul Derbyshire: paul.derbyshire/at/bbsrc.ac.uk; Maureen C McCann: mmcann/at/bilbo.bio.purdue.edu; Keith Roberts: keith.roberts/at/bbsrc.ac.uk

Received February 14, 2007; Accepted June 17, 2007.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

This article has been cited by other articles in PMC.

Abstract

Background

Cell elongation is mainly limited by the extensibility of the cell wall. Dicotyledonous primary (growing) cell walls contain cellulose, xyloglucan, pectin and proteins, but little is known about how each polymer class contributes to the cell wall mechanical properties that control extensibility.

Results

We present evidence that the degree of pectin methyl-esterification (DE%) limits cell growth, and that a minimum level of about 60% DE is required for normal cell elongation in Arabidopsis hypocotyls. When the average DE% falls below this level, as in two gibberellic acid (GA) mutants ga1-3 and gai, and plants expressing pectin methyl-esterase (PME1) from Aspergillus aculeatus, then hypocotyl elongation is reduced.

Conclusion

Low average levels of pectin DE% are associated with reduced cell elongation, implicating PMEs, the enzymes that regulate DE%, in the cell elongation process and in responses to GA. At high average DE% other components of the cell wall limit GA-induced growth.

Background

Young, dividing and expanding cells are surrounded by an extensible primary wall that can allow turgor-driven increases in cell volume. In dicotyledonous plants, primary cell walls are composed of two major interpenetrating polysaccharide networks of cellulose-xyloglucan and pectin, in roughly equal proportions, but the contribution that each polymer class makes to wall extensibility is not yet understood.

The cellulose-xyloglucan network is considered to be the major load-bearing structure [1,2]. Cellulose microfibrils are generally oriented perpendicular to the direction of cell expansion and, because of their tensile strength, define an axis of growth by limiting radial expansion [3]. Breaking and reforming of the xyloglucan chains, that inter-connect cellulose microfibrils, by wall glucanases [4] and xyloglucan-endotransglycosylases (XETs) [5,6], and/or disruption of attachment sites between cellulose and xyloglucan by expansins [7], may then promote longitudinal growth through slippage of the microfibrils. However, little is known about how the surrounding pectin matrix might play a role in this process, either independently or in concert with the cellulose-xyloglucan network. A unique property of pectin is its ability to form gels with varying mechanical strength. Removal of methyl-esters from the pectic galacturonic acid residues by pectin methyl-esterase (PME) [8] creates negatively charged regions of the homogalacturonan (HG) backbone. Depending upon the extent and pattern of de-esterification, these can coordinate with divalent metal ions such as calcium and promote cross-links [9,10], or generate swelling forces through mutual electrostatic repulsion [11]. These two forces exert opposing effects but can have a major influence over the gelling properties of pectin, and a profound effect on wall extensibility. Indeed, the spatial variation in methyl-esterification levels at intercellular spaces suggests that HG has an in vivo mechanical role within the cell wall [12] and contributes to the mechanical properties to the wall. Rhamnogalacturonan II-borate di-di-ester cross-links have also been shown to be load-bearing in tensile strength assays of Arabidopsis hypocotyls [13].

Methyl, acetyl, phenolic and other unidentified ester linkages in varying proportions represent the ester content of HG, and a relationship between primary wall pectin esterification and cell expansion has been described in a variety of systems. An early study, using ruthenium red to stain negatively charged carboxyl groups of HG, showed the stain was strongest in the basal part of sunflower (Helianthus annuus) hypocotyls, where cell elongation had slowed or stopped, whereas further up the hypocotyl, cells continued to elongate and ruthenium red staining was relatively weaker [14]. Similarly, along the axis of mung bean (Vigna radiata) hypocotyls, elongating regions have elevated levels of highly methyl-esterified pectins, in contrast to basal regions that have stopped growing and contain fewer esterified HG residues [15]. Highly methyl-esterified regions also have walls that are more plastic, with reduced PME activity, as opposed to mature, stiffer walls at the base of the hypocotyl where PME activity is higher [16]. More recently, direct biochemical analysis in maize (Zea mays) showed that total cell wall ester content rises during coleoptile elongation and then falls as growth ceases, but the proportion of methyl-esters is not changed [17]. Similarly, a sharp rise in methyl-esterification occurs when tobacco (Nicotiana tabaccum) cell suspension cells elongate, but is at a lower constant level prior to this [18]. The degree of esterification DE% falls in cells that have completed the elongation phase, however, methyl-esters are unchanged and a fall in other esters must account for the reduced DE%. Thus, in tobacco suspensions, methyl-esterification levels may regulate the onset of cell elongation, but are not necessarily involved in cessation of elongation. Likewise, differences in the composition and architecture of type I and type II cell walls [1] may reflect the differing roles that alternative ester groups might play in regulating wall extensibility.

Genetic manipulation of PMEs using over-expression studies has recently allowed the link between DE% and cell expansion to be tested further, but has given more complex results. Potato (Solanum tuberosum) plants over-expressing a putative PME from Petunia inflata showed increased PME activity in leaves and tubers but did not affect DE%, whereas cell wall ion binding capacity was affected in tubers and yield was reduced [19]. Similarly, antisense inhibition of a putative PME in pea (Pisum sativum) roots increased extracellular pH and inhibited root cap border-cell separation leading to stunted root growth, but effects on DE% were not reported [20]. In contrast, expression of an Aspergillus niger PME in tobacco reduced the proportion of methyl-esters in pectin and reduced cell size, creating dwarf plants [21]. PMEs therefore appear to have diverse roles in wall metabolism and plant development.

The Arabidopsis hypocotyl has been widely used to study the effects of light and hormones on plant growth responses [22,23]. It is also an appropriate system in which to study cell elongation, since it grows almost exclusively by cell expansion and is essentially division-free [24-26]. In this paper, we use two well-characterised gibberellic acid (GA) mutants to identify cell wall compositional changes that may be related to the inhibition of hypocotyl elongation. The GA-deficient ga1-3 is a loss of function mutant in the GA1 gene which encodes an enzyme involved in GA biosynthesis [27-29]. As a result, ga1-3 has reduced amounts of GA [30] and is severely dwarfed, but can be rescued by an exogenous supply of GA [29]. The semi-dominant gai mutant has a similar dwarf phenotype to ga1-3 but cannot be rescued by exogenous GA [31]. GAI is a member of the DELLA family of putative transcription factors, key components of GA-signalling [32]. GAI and other members of this family (RGA/RGL) act as repressors of plant growth, but are themselves repressed in the presence of endogenous GAs [33,34]. Thus, in ga1-3 all DELLA proteins are active. In gai, a 17 amino acid deletion in the DELLA region of GAI alters the structure and function of the protein such that it can no longer be repressed by GA [33,35].

Using these two mutants, and particularly the conditional rescue of cell elongation by GA in the ga1-3 mutant, we show that active cell elongation is associated with a higher average level of pectin esterification. If DE% is reduced by the over-expression of a well-characterised fungal PME, then cell elongation is decreased.

Results

Hypocotyl growth kinetics in two dwarf GA mutants

ga1-3 provides a system in which cell elongation in the hypocotyl can be rescued conditionally by exogenous application of GA, while gai provides a control for the effects of exogenous GA application. Hypocotyl growth kinetics in wild-type (WT) (Ler), ga1-3, and gai seedlings were established in a continuous light environment with plates positioned horizontally. Hypocotyl growth was measured during a period of 10 d after the culture plates were transferred to the growth room, in the presence and absence of 1 μM exogenous GA₄(Figure (Figure1A),1A), a concentration that restores hypocotyl length of ga1-3 to WT length [36]. In the absence of exogenous GA, WT hypocotyls elongate between 2 and 7 d, and have a final length of around 2 mm. ga1-3 required an extra day to germinate, after which hypocotyl elongation was minimal, reaching only 0.6 mm. gai hypocotyls elongate for up to 6 d, but at a slower rate than the WT, with a maximum length of about 1.6 mm. In the presence of exogenous GA, WT hypocotyls elongate between 2 and 7 d, and have final lengths of approximately 3.5 mm, and such hypocotyls grow longer and at a faster rate than without GA. ga1-3 hypocotyls respond to exogenous GA, elongating for up to 7 d, with final lengths of around 3 mm. Finally, gai does not respond to exogenous GA, having the same hypocotyl growth kinetics and final length as in the absence of the growth regulator, thus confirming its insensitivity to GA. These results are consistent with those reported previously [36]. However, in our analysis, final hypocotyl lengths are shorter, probably as a consequence of the inhibitory effects of the continuous light regime used.

Figure 1

Growth kinetics and hypocotyl cell elongation in WT (Ler), ga1-3, and gai seedlings grown with and without exogenous gibberellic acid (GA). (A) Seedlings were grown in continuous light for 10 d with plates in a horizontal position and hypocotyl growth (more ...)

Our analysis of WT, ga1-3, and gai hypocotyls and their cell walls used material taken at an equivalent developmental stage; in our case defined as approximately 50% of final hypocotyl length, estimated from the growth curves in Figure Figure1A1A and indicated by arrows. This was set at 3 d, both in the presence and absence of GA. However, for ga1-3, in the absence of GA, hypocotyls barely grow following germination. Therefore we analysed hypocotyls at 3 d, the earliest time point following germination. The general morphology of 3-d-old seedlings of average hypocotyl length is shown in Figure Figure1B.1B. In the absence of exogenous GA, WT hypocotyls are approximately 1 mm long, but are almost twice as long (1.8 mm) when grown in the presence of exogenous GA. In contrast, ga1-3 seedlings are severely dwarfed with hypocotyls at approximately 0.5 mm in length. When grown in the presence of exogenous GA, ga1-3 hypocotyl length is restored to that of untreated WT. In the absence of GA, gai seedlings have slightly shorter hypocotyls than WT, at about 0.8 mm, and are unaffected by exogenous GA. GA-regulation of hypocotyl growth is mediated through elongation of the pre-existing cells with little or no contribution from cell division [36]. To test whether continuous light affects this process, epidermal cells were imaged with a field-emission scanning electron microscope (FESEM) (Figure (Figure1B).1B). In the absence of exogenous GA, WT epidermal cells are almost twice as long as those of ga1-3, while gai epidermal cells are slightly shorter than WT. In the presence of exogenous GA, WT epidermal cells approximately double in length, ga1-3 epidermal cell length is increased 2 to 3 fold and gai epidermal cell length is unchanged. The relative differences in epidermal cell length closely match the relative differences in hypocotyl length. As the same relative differences in cell length have also been observed in the cortical and endodermal layers [37], the differences in hypocotyl length are likely to reflect differences in cell length and therefore in cell elongation.

Fourier Transform Infrared (FTIR) microspectroscopy of WT and mutant hypocotyls

FTIR microspectroscopy has been used to measure the composition of plant cell walls [38-40]. Small areas of tissue can be selected for analysis, and other advantages include the speed of both sample preparation and data collection. We used FTIR microspectroscopy to quickly ascertain if DE% was associated with dwarfism in primary cell walls of Arabidopsis hypocotyls. Spectra were collected from a 200 × 100 μm area in the central region along the length of WT and ga1-3 hypocotyls, grown in the presence and absence of exogenous GA and at the developmental stages indicated in Figure Figure1B.1B. The central stele was avoided to prevent contamination from secondary cell wall components. For each population of hypocotyls, DE% was determined semi-quantitatively based on the method described by Filippov and Kohn [41]. Table Table11 shows cell walls of WT hypocotyls have a DE of about 60% when grown both in the presence and absence of GA. In contrast, DE is lowest in walls of ga1-3 hypocotyls grown without GA, at about 40%, but rises to around 55% when grown in the presence of GA. Thus, GA-promoted cell elongation in ga1-3 hypocotyls is associated with a corresponding rise in DE%.

Table 1

Semi-quantitative determination of DE% in WT and ga1-3 hypocotyl cell walls.

Biochemical analysis of hypocotyl cell walls

To more accurately determine pectin DE%, we measured HG content as uronic acid, and methyl-ester content as the amount of methanol released, at the developmental stages described in Figure Figure1B.1B. Average hypocotyl lengths used in all experiments are shown in Figure Figure2A.2A. When grown without exogenous GA, WT (Ler) hypocotyls measured 1.06 ± 0.02 mm, and increased to 1.74 ± 0.02 mm in the presence of GA. Dwarf ga1-3 hypocotyls were 0.55 ± 0.02 mm but increased to 1.31 ± 0.03 mm with exogenous GA. Finally, gai hypocotyls measured 0.82 ± 0.01 and 0.86 ± 0.01 mm, when grown without or with GA respectively. Uronic acid and methanol content are expressed as amount per hypocotyl. Since hypocotyl growth is essentially division-free, a change in the amount of a particular wall component can be correlated primarily to cell elongation.

Figure 2

Effects of gibberellic acid (GA) on degree of esterification (DE%) in WT (Ler), ga1-3 and gai hypocotyl cell walls. (A) Hypocotyl length at time of excision in 3-d-old seedlings. Measurements are an average of 40 to 90 hypocotyls ± SE for each (more ...)

When grown in the absence or presence of GA, WT uronic acid content was 2.31 ± 0.09 and 2.43 ± 0.10 nmol per hypocotyl, respectively, and so was not significantly different between the two treatments (Figure (Figure2B).2B). In ga1-3 hypocotyls, grown without GA, the values were lower than WT, measuring 2.00 ± 0.16 nmol per hypocotyl, and was unchanged at 2.10 ± 0.11 nmol per hypocotyl when grown in the presence of GA. gai hypocotyls contained the lowest amount of uronic acid, at 1.81 ± 0.04 and 1.75 ± 0.09 nmol per hypocotyl when grown without or with GA respectively. As with WT and ga1-3, GA did not affect the uronic content of gai hypocotyls. GA also did not significantly affect methanol released in WT. In the absence of GA, methanol released was 1.38 ± 0.04 nmol per hypocotyl. When grown in the presence of exogenous GA, methanol released from WT was 1.44 ± 0.03 nmol per hypocotyl. Therefore, GA affected the amount of neither uronic acid nor methanol released in WT cell walls, even though hypocotyl length increased almost two-fold over the same period of growth. In contrast, GA increased the amount of methanol released from ga1-3 cell walls, rising from 0.97 ± 0.08 nmol per hypocotyl in the absence of exogenous GA, to 1.23 ± 0.06 nmol per hypocotyl when grown in the presence of GA. GA-stimulated growth therefore correlates with an increase in cell wall methyl-esterification. Finally, gai hypocotyls contained similarly reduced amounts of methanol to ga1-3, at 0.97 ± 0.05 nmol per hypocotyl when grown without GA, and was not significantly altered with GA, at 0.91 ± 0.04 nmol per hypocotyl.

The ratio of methanol to uronic acid content was used to calculate DE% (Figure (Figure2C).2C). In WT hypocotyls this was 60.04 ± 2.23% and 59.08 ± 2.31% in the absence and presence of exogenous GA, respectively. GA therefore promotes cell elongation and hypocotyl growth in WT but does not affect DE%. In contrast, GA did affect DE% in ga1-3 hypocotyls. In the absence of exogenous GA, DE was 48.23 ± 4.00%, rising to 58.89 ± 3.12% when grown in the presence of GA. GA-stimulated growth in the dwarf ga1-3 hypocotyls therefore correlated with the recovery of DE% to WT levels in this mutant. In the semi-dwarf hypocotyls of gai, DE was 53.91 ± 1.08 and 52.25 ± 2.52% when grown either without or with GA, respectively. A correlation therefore exists, between hypocotyl length and DE%. The shortest hypocotyls of ga1-3 have the lowest DE%, but stimulation of hypocotyl extension by GA also increases DE% to the WT level. gai hypocotyl length is intermediary between ga1-3 and WT regardless of GA, as is the measured DE% in this mutant.

In summary, an increase in hypocotyl length, and therefore cell elongation, is also accompanied by an increase in DE%. However, enhanced growth of WT induced by GA does not affect DE%. These data suggest that the degree of pectin esterification may affect cell elongation in a GA-deficient and GA-insensitive background.

Heterologous PME expression reduces hypocotyl length and DE%

To directly test our hypothesis that a low average DE% may constrain growth, we artificially manipulated DE% using reverse genetics. Our prediction would be that reducing the DE% should inhibit hypocotyl elongation. T-DNA insertions into putative PMEs might in principle reduce the potential for de-esterification and ionic cross-linking, leading to an increase in wall extensibility. In Arabidopsis, 67 putative PMEs, in Carbohydrate Esterase Family 8, have been identified based on protein sequences [42]. Therefore, the scope for functional redundancy in this family is high, and gene knock-outs might not reveal clear phenotypes. In addition, no PMEs have been biochemically characterised in this species, and some may actually be pectin trans-esterases [43,44]. For the same reasons, homologous over-expression of endogenous or other plant putative PMEs, without biochemical characterisation, may give results that are difficult to interpret [19,20]. In contrast, several bona fide PMEs have been reported in bacteria and fungi [45,46]. In Aspergillus aculeatus, the PME1 gene has been rigorously tested and biochemically characterised [47]. We therefore transformed the PME1 cDNA clone into Arabidopsis under the control of a constitutive promoter. Interestingly, constitutive expression of PME1 yielded no transformants and therefore is probably lethal.

Analysis of the predicted signal peptide region using pSORT showed a low probability of the PME1 protein localising to the cell wall in plants. Therefore, we removed the signal peptide sequence and replaced it with one from a putative PME from Arabidopsis (At4g12390) that had a high probability of targeting the protein to the cell wall. The ethanol-inducible expression system was used [48], in which the chimeric construct was cloned downstream of the AlcA promoter, and then transformed into line P5-3 carrying the AlcR promoter. Several independent lines carrying the transgene were identified by PCR using gene-specific primers. To induce expression of the transgene, seedlings were grown for 3 d in continuous light with plates in a near vertical position, and then transferred to induction medium containing 0.1% ethanol in the solidified medium. Transfer at this time point, allowed germination to take place and hypocotyls to enter the rapid phase of elongation. Two lines, PME01 and PME08, in which hypocotyl growth was affected only in the presence of ethanol, were selected for further analysis.

Hypocotyl growth kinetics are shown in Figure Figure3.3. In the absence of ethanol, P5-3 hypocotyls grew over a period of 6 d, from day 2 to day 8, with a final length of 5.56 ± 0.17 mm (Figure (Figure3A).3A). The concentration of ethanol used to induce PME1 expression did not affect either the growth profile or final length of P5-3 hypocotyls, which measured 5.77 ± 0.29 mm at day 10. However, compared to previous experiments (Figure (Figure1A),1A), the duration and extent of hypocotyl elongation was increased when plates were positioned vertically, and may be the result of additional nutrient uptake and/or touch responses from being in contact with the surface of the growing medium. In the absence of ethanol, both PME01 and PME08 hypocotyls followed a similar growth profile as P5-3. Final lengths were 5.67 ± 0.22 and 5.25 ± 0.21 mm in lines PME01 and PME08, respectively (Figure 3B, C). However, transfer of the seedlings to induction medium resulted in a deflection of the growth curve for both expressing lines. Hypocotyls stopped growing about 1 d earlier, and final lengths were 4.63 ± 0.24 and 4.27 ± 0.23 mm, respectively, representing a length reduction of about 20%.

Figure 3

Growth kinetics and hypocotyl cell elongation in P5-3, PME01, and PME08 seedlings. Seedlings were grown in continuous light for 10 d with plates in a near vertical position and hypocotyl growth measured over this period. Measurements are an average taken (more ...)

Transcriptional and cell wall analysis was performed on excised hypocotyls after 2 d growing on control/induction medium (arrows in Figure Figure3).3). At this time point (day 5), the A. aculeatus PME was strongly expressed in both lines when grown in the presence of ethanol, whereas no expression was detected in seedlings grown on ethanol-free medium or in P5-3 (Figure (Figure4).4). Expression was stronger in line PME08 compared to PME01. Both parental lines had reduced seed yield, which may be a consequence of auto-induced PME1 expression during seed set, and/or during pollen tetrad separation, the latter involving PME [49]. Thus, it was difficult to collect enough transgenic hypocotyls for direct chemical analysis. Therefore, to confirm that the growth effects were due to pectin de-esterification, we again used FTIR microspectroscopy of individual hypocotyls to measure DE% indirectly (Table (Table2).2). At this time point, hypocotyl lengths in P5-3 were 4.52 ± 0.19 and 4.46 ± 0.30 mm when grown in the absence and presence of ethanol, respectively. In the absence of ethanol, PME01 hypocotyls were 4.25 ± 0.19 mm long, compared to 3.60 ± 0.24 mm when grown on induction medium. Similarly, PME08 hypocotyls were 4.08 ± 0.33 and 3.15 ± 0.29 mm after 2 d growth on control and induction medium, respectively. Induced expression of PME1 therefore corresponded to a 15% reduction in average hypocotyl length in line PME01, and a 22% reduction in line PME08, compared to non-induced seedlings. DE in P5-3 hypocotyls was about 48% in the absence of ethanol, and about 45% in the presence (Table (Table2).2). In line PME01, DE was about 48% in the absence of ethanol, but only about 40% following induction. In line PME08, DE was about 42% in the absence of ethanol, and reduced to about 38% when induced. The overall reduction in DE in P5-3, from about 60% (Table (Table1)1) to about 48% (Table (Table2),2), may be due to the slowing down of hypocotyl elongation at day 5, as opposed to day 3 when they are growing fastest. Nevertheless, the lowest DE% we measured, in both lines, followed PME1 induction. In summary, PME1 expression corresponded to a reduction in cell wall DE% and hypocotyl length in both lines. Expression was strongest in line PME08 in which we measured both the lowest DE% and the shortest hypocotyls.

Figure 4

Transcriptional analysis of PME1 using RT-PCR. RNA was extracted from hypocotyls after 2 d growth on control/induction medium (arrows in Figure 3) and reverse transcribed. PME1 expression was detected using gene-specific primers to amplify a 932 bp product. (more ...)

Table 2

Semi-quantitative determination of DE% in P5-3, PME01 and PME08 hypocotyl cell walls.

Discussion

In this work, we used the hypocotyl of the ga1-3 mutant, as a system in which we can induce cell elongation, to investigate the relationship between the level of pectin esterification and cell elongation. We measured low DE% in this dwarf GA-deficient mutant, and a high average DE% in WT hypocotyl cell walls. Intermediate DE% between ga1-3 and WT were found in the GA-insensitive mutant gai that correlated with its semi-dwarf hypocotyl, and GA-induced growth of ga1-3 was paralleled by a recovery of DE% to WT. However, further increases in WT hypocotyl growth, induced by GA, were not accompanied by further changes in DE% above the maximum. This suggests that a permissive level of DE% exists in the primary cell wall of Arabidopsis hypocotyls, and that a reduction in average DE% below this level progressively reduces cell elongation. Above this level, other factors become limiting for growth. Reducing DE%, by alcohol-induced expression of PME1 from A. aculeatus, resulted in a predicted inhibition of hypocotyl growth. Since endogenous PMEs are responsible for the removal of methyl-esters from cell wall pectin, we predict that one or more members of this family of enzymes plays a role in regulating cell elongation in vivo.

Pectin is synthesised and deposited in the wall in a highly methyl-esterified form [50], with measurements as high as 80% DE [17,18]. In Arabidopsis hypocotyls we measured maximal DE of ~60% (Figure (Figure2C),2C), and, it is likely that pectin is synthesised at values above this and subsequently de-esterified to a level where it is maintained. At this level, pectin may be at the optimal DE% to contribute to wall plasticity and thus to cell elongation, but de-esterification to levels below this progressively restricts plasticity and hence hypocotyl growth. Current theories of how DE% may regulate wall extensibility, and thus cell expansion, are largely based on in vitro studies of pectin gels. Pectin has a highly complex macromolecular structure, and its properties can be modulated by several factors that include pH, osmolarity and ionic conditions [11]. One of the main influences of DE% is regulating the amount of ionised stretches of the HG backbone that can cross-link with calcium ions [9]. A reduction in DE% increases the potential for such cross-links and leads to a more rigid gel with increased visco-elastic properties [12,51]. This may independently affect the extensibility of the cell wall, but may also act by modifying the mechanical properties of the key load-bearing polymers, the cellulose-xyloglucan network. The presence of pectin increases the extensibility, and reduces the stiffness, of cellulose-pectin composites, compared to cellulose alone, with low DE systems (30%) having a greater effect than high DE systems (67%) [52]. Therefore, if wall extensibility is indeed affected by the physico-mechanical properties imposed by DE%, these effects may be autonomous to the pectin network. Indeed, linear stretching experiments show that the pectin network moves independently of the cellulose-xyloglucan network [53,54].

Plant PMEs are thought to remove methyl-ester groups in a blockwise fashion, leading to contiguous stretches of free carboxyl residues within the HG backbone, whereas fungal PMEs are thought to de-esterify pectin randomly resulting in single carboxyl residues that are dispersed throughout the HG portion of pectin [55,56]. The resulting pattern of de-esterification can have different effects on pectin properties. Blockwise de-esterification favours cross-linking [9], requiring at least 9 contiguous carboxyl residues for coordination with calcium [57]. In contrast, random de-esterification may promote swelling, and reduces wall porosity [12]. In vitro studies have been performed on calcium-pectin gels with similar DE% but de-esterified either by plant or by fungal PMEs. Gels prepared from fungal PME-treated pectin have no capacity to recover under compression, whereas they recover completely when de-esterified by plant PMEs [12]. Both mode and extent of de-esterification can therefore influence the rheological properties of pectin, and can potentially regulate wall extensibility but by different mechanisms. At an optimum pH of 4.6, PME1 is highly effective at de-esterification, removing 75–85% of methyl groups in vitro [47]. However, in our study it is unlikely that PME1 had a major impact on DE% in hypocotyl cell walls, since indirect measurements showed only modest reductions, i.e., from about 48% to 40% in PME01, and about 42% to 38% in PME08 (Table (Table2).2). This may be the result of duration of expression, sub-optimal wall pH and/or accessibility to HG within the cell wall matrix. Therefore, expression of PME1 from A. aculeatus may have resulted in random de-esterification and affected wall loosening properties more through a reduction in pore space, possibly caused by electrostatic repulsion of fixed negative charges, leading to swelling of the pectin network and more efficient filling of the available spaces [11,58], and reduced porosity may subsequently limit accessibility of wall loosening proteins to their cellulose-xyloglucan substrate. Similarly, inhibition of hypocotyl elongation in ga1-3 and gai may be due to cross-linking of the pectin network giving stiffer walls, with less effect on pore space. It is important to recognise that we are looking at small effects with this experimental system. High levels of PME are likely to be lethal, and low levels, coupled with random patterns of de-esterification, are likely to have small effects. Nevertheless the tight correlation of extension with DE% is clear. Further studies of the loss- and gain-of-function mutants described here may help to identify any differences in pectin structure that are the result of GA-deficiency/insensitivity, compared to effects of PME1 expression.

Since we do not know exactly which polymers are affected by PME1, or where, it is important to consider that small changes in some crucially located pectin molecules may underlie the effects we measured. One possibility is that middle-lamella pectin, which in general is highly de-esterified, may act as a trans-cellular brake, helping coordinate differential growth between adjacent cells to achieve even growth in the organ as a whole [57]. Another possibility, reflecting our awareness that it is probably just the outer epidermal wall that both drives and constrains growth of the hypocotyl [59], is that the pectin in this very thick outer wall [60] alone is involved in the relationship between growth and pectin DE%.

Other studies in which plant PMEs have been constitutively over-expressed have given more complex results. In pea, inhibiting the expression of a PME altered cell wall pH and inhibited the loss of root cap border cells, resulting in swollen roots and reduced elongation [20]. More recently, over-expression of a Petunia inflata PME in potatoes caused a transient increase in stem elongation in regions with reduced PME activity [19]. According to the authors, the reduction in PME activity may have been caused by compensation for the effects of over-expression, however down-regulation of PME and increase in stem elongation is consistent with the hypothesis presented here. Neither of these putative PMEs, or indeed any other plant PMEs, have been characterised biochemically so their mechanistic effects on growth remain speculative. In contrast, PME1 has been functionally characterised [47], and the inducible system we used [48] gave tight control over its expression. Likewise, a reduction in DE% and production of dwarf tobacco plants resulted when a functionally characterised PME from Aspergillus niger was over-expressed [21], further emphasising the need for more rigorous characterisation of these plant enzymes prior to their manipulation. Over-expression of plant-derived PMEs in plants may also be compromised by the presence of endogenous PME inhibitors (PMEIs), a recently identified family of proteins that adds another regulatory level to pectin metabolism and DE% [61-63]. Indeed, over-expression of PMEIs in Arabidopsis resulted in a decrease in overall PME activity coupled with an increase in DE%. Transgenic seedlings, consistent with our hypothesis, also produced longer roots and had longer cells in the elongation zone of the root [64].

While GA promoted elongation in WT hypocotyls, it did so with no net increase in cell wall uronic acid content over the same growth period (Figure (Figure2B).2B). Elongation in this case correlates with cell wall thinning [60]. Maintaining DE% at an adequate level may therefore contribute to the strength of the thinning wall, as well as to its extensibility. Similarly, GA-recovery of hypocotyl growth and DE% in ga1-3 does not increase net uronic acid content of the dwarf hypocotyl. Taken together, our data suggests that GA also promotes cell elongation via remodelling of the existing wall. Putative wall loosening proteins have been shown to be GA-regulated. For example, GA enhances cell expansion and glucanase activity in maize leaves [65] and wheat (Triticum aestivum) internodes [66], and an XET is GA-regulated in germinating tomato (Lycopersicon esculentum) seedlings [67]. This correlates with increases in wall extensibility that are not seen in GA-insensitive wheat cultivars [66,68]. GA also increases wall extensibility in lettuce (Lactuca sativa) [69] and cucumber (Cucumis sativus) hypocotyls [70]. Therefore, in Arabidopsis hypocotyls, GA may also promote cell elongation by loosening of the cellulose-xyloglucan network in conjunction with wall remodelling, and restrict it by modulating DE%. In lettuce hypocotyls [71], oat (Avena sativa) [72] and wheat internodes [66], both net cell wall polysaccharide and organ elongation are simultaneously increased by GA. Thus, synthesis and deposition versus remodelling of the cell wall during GA-stimulated cell expansion may vary, depending upon the plant species. Relative to WT hypocotyls, uronic acid content was reduced in ga1-3 and lowest in gai. Therefore, both GA1 and GAI are required for normal uronic acid incorporation into the wall, as well as for controlling its methyl-ester content.

Conclusion

We have shown a consistent relationship between the average degree of cell wall pectin esterification (DE%) and the degree of cell elongation in Arabidopsis hypocotyls. A reduction in hypocotyl length, using either forward or reverse genetic approaches, is associated with a reduction in DE%. Endogenous PMEs and their inhibitors, which regulate the DE%, are therefore implicated in cell elongation in this system. GA has no effect on DE% in WT hypocotyls but promotes additional cell elongation, suggesting that enzymes regulating the cellulose-xyloglucan network and other components of the primary cell wall may be involved in responses to the growth regulator.

Methods

Plant materials and growth conditions

Arabidopsis thaliana (L. Heynh) ecotype Landsberg erecta (Ler) was used as the reference wild-type (WT). In the over-expression experiment, line P5-3 (also in the Ler background) was used as WT. Seeds were surface-sterilised by immersion for 5 min in 5% (v/v) Vortex bleach (Procter & Gamble Ltd, containing 5 to 15% chlorine-based bleach), and washed three times in sterile distilled water (sdH₂O). Following sterilisation, to allow seeds of ga1-3 to germinate, they were incubated at 4°C for 5 d in a solution of 1 μM GA₄(Sigma-Aldrich, UK) [36]. Ler and gai do not require this treatment but were included for consistency. Next, seeds were rinsed five times in sdH₂O and sown onto medium containing 1× Murashige and Skoog (MS) basal salts (micro and macro elements) (Duchefa) supplemented with 3% (w/v) sucrose (pH adjusted to 5.7) and solidified with 0.5% (w/v) Phytagel™ (Sigma-Aldrich, UK). Approximately 20 seeds were evenly sown per 9 cm Petri plate (Bibby Sterilin Ltd) containing 20 mL of growing medium, and plates sealed with Parafilm^®laboratory film (Pechiney Plastic Packaging, Menasha, USA). Plates were placed in darkness at 4°C for 48 h to stimulate and synchronise germination. Following cold treatment, plates were transferred to a growth room maintained at 25°C and incubated horizontally under fluorescent lamps (70 μmol m^-2s^-1) in a continuous white light regime.

Hypocotyl measurements

Hypocotyl length was determined as the distance between the top of the collet root hairs, to the 'V' made by the cotyledon shoulder [73]. Hypocotyl lengths were measured using a Leica WILD M10 binocular microscope fitted with an eye-piece graticule, and the mean ± SE calculated for each data set.

Field emission scanning electron microscopy (FESEM)

Seedlings were mounted in a horizontal position on adhesive carbon tabs (Agar Scientific Ltd) and plunge-frozen at -210°C in liquid nitrogen slush. After freezing, samples were immediately loaded into the cryo chamber of the scanning electron microscope, equilibrated with the stage and sublimed at -100°C for 2 min. The temperature was returned to -110°C, the samples were sputter-coated with platinum for 2 min at 10 mA, and then transferred to the imaging stage at -130 to -150°C for analysis. FESEM images of hypocotyl epidermal cells were obtained using a Philips XL30 FEG scanning electron microscope (FEI Co., Eindhoven, The Netherlands) fitted with a cryostage (CT1500 HF; Oxford Instruments, Abingdon, Oxford, UK), operating at 3 kV and a working distance of between 5 and 15 mm.

FTIR microspectroscopy

Whole hypocotyls were excised from seedlings and suspended on the surface of water-soaked tissue paper to prevent tissue dehydration during sample collection. This also effectively rinsed the samples. The samples were compressed onto barium fluoride (BaF₂) windows (13 × 2 mm) (Crystran Ltd, Poole, UK), dried at 60°C for 1 h and used immediately for spectral acquisition, or stored overnight at 4°C and used the next day. Windows were supported on the stage of a UMA500 microscope accessory of a Bio-Rad FTS175c spectrometer equipped with a liquid nitrogen-cooled mercury cadmium telluride detector and absorbance spectra obtained. Sixty-four interferograms were collected in transmission mode with 8 cm^-1resolution and co-added to improve the signal-to-noise-ratio for each sample. An area of approximately 200 × 100 μm in the middle region (along the longitudinal axis) of each hypocotyl was selected, avoiding the central stele. One spectrum was collected from each hypocotyl and between 10 and 28 samples for each genotype/treatment used. For each population the spectra were averaged between 790 and 1810 cm^-1and each average spectrum baseline-corrected and area-normalised to account for differences in sample thickness. Processing of spectral data was done using OMNIC E.S.P. 5.0 software. For each spectrum, a two-point baseline was constructed between 870 and 1810 cm^-1. The absorbance maxima of bands υ_as(COO^-) 1605 cm^-1and υ(C = O)_ester1745 cm^-1from the baseline were measured, and the log ratio of these values used to semi-quantitatively calculate DE% from the calibration curve of Filippov and Kohn [41]. For each genotype/treatment, values were averaged ± SE.

Uronic acid and methyl ester assays

Hypocotyls were excised precisely using fine-tipped forceps and a razor blade. Upon excision, samples were transferred to a 1.7 mL microfuge tube containing 1 mL absolute ethanol and heated to 85°C for 20 min to extract chlorophyll, sugars and other small molecules. An additional extraction was made in 1 mL 80% (v/v) ethanol at 85°C for a further 20 min, and then rinsed three times in 1 mL sdH₂O. Samples were suspended in a small volume of sdH₂O and freeze-dried. Each tube contained between 50 and 100 hypocotyls. Uronic acid assays were performed on these as described previously [74]. Methyl-esters were determined as the amount of methanol released following saponification using the method described by Kim and Carpita [17]. Values are expressed as nmol per hypocotyl. For each genotype and treatment, duplicate or triplicate samples were used in each experiment, and each experiment performed three times. In total, 600–900 hypocotyls were used to independently calculate average uronic acid and average methanol values. The ratio of methanol to uronic acid was used to calculate DE%. Thus in total, between 1200 and 1800 hypocotyls were used to derive the average DE% for each genotype/treatment. Standard error values were ratioed as described previously [75].

Construction of plasmids and plant transformation

The open reading frame of PME1 (Accession no: U49378) from Aspergillus aculeatus [47], minus the predicted signal peptide sequence, was PCR amplified out of pYES 2.0 using the forward primer OVEXP3 (5'-CTGCCAATCCACCATAGCCGCCAGCCGTACCACGGCTCC-3') and the reverse primer OVEXP4 (5'-GGCGAATTCTTTAATTAGAAGTAGGAGGTATCGAC-3'). The underlined region denotes the EcoRI restriction site. The signal peptide sequence of a putative PME (At4g12390) from Arabidopsis was PCR amplified from BAC clone T4C9 (supplied by ABRC) using the forward primer OVEXP1 (5'-GGCGGATCCTTATGGAACCAAAGCTAACCCA-3') and the reverse primer OVEXP2 (5'-GGAGCCGTGGTACGGCTGGCGGCTATGGTGGATTGGCAG-3'). The underlined region denotes the BamHI restriction site. The plant signal peptide sequence was ligated to the fungal PME sequence giving a 1133 bp cDNA product, and then digested with BamHI/EcoRI and ligated into pL4 upstream of the AlcA35S promoter and downstream of CaMV35S terminator. The vector was linearised by digesting with BglII, followed by a second digestion with HindIII to give a 1696 bp fragment containing the AlcA35S::PME::CaMV35S terminator region. The gel-purified product was ligated into pGreen0229 using HindIII/BamHI and the chimeric construct transformed via Agrobacterium tumefaciens (GV3101) into line P5-3 (containing the ethanol-inducible AlcR promoter) using the floral-dip method [76]. Tranformants were selected with Basta and T2 plants used for phenotypic analysis.

Plant growth and ethanol induction

Seeds were prepared as described above and sown onto sterile filter paper in contact with growing medium containing 1% (w/v) sucrose. Sealed plates were incubated in a near vertical position. This allowed hypocotyls to be measured each day without opening plates, which would have resulted in some loss of ethanol vapour (see below). After 3 d seedlings were carefully transferred to the same medium containing no ethanol (control medium) or to induction medium containing 0.1% (v/v) ethanol. Induction medium was prepared by adding the appropriate volume of 50% (v/v) of ethanol to the molten medium cooled just to the point at which it started to solidify in order to prevent ethanol evaporation. Following transfer, plates were resealed with Parafilm. Hypocotyl lengths were imaged digitally and measured using Photoshop 5.0 software.

Transcription analysis by RT-PCR

RNA was extracted from whole seedlings at 2 d after transfer to induction/control medium, using a QIAGEN RNeasy Plant minikit according to the manufacturer's instructions. RNA yield was quantified by spectrophotometry and concentrations equalised with RNase-free water. After DNase treatment (40 units DNaseI; Amersham Pharmacia) for 20 min at 37°C, 2.5 μg was reverse transcribed for 60 min at 42°C in a final volume of 20 μL in the presence of 20 units RNA guard, 1 mM dNTPs, 5 mM MgCl₂, 0.3 μM oligo(dT) primers and 4 units M-MLV reverse transcriptase (Life Technologies) in the reaction buffer provided. Reactions were stopped by heat inactivation and 80 μL H₂O added. 2 μL of the reverse transcription reaction were used for PCR amplification. The forward primer PMEfor (5'-GTACCACGGCTCCCTCCG-3') and the reverse primer PMErev (5'-GTAGGAGGTATCGACCCAGC-3') gave a 932 bp product for the transgene cDNA. The forward primer Actin2-5' (5'-CTAAGCTCTCAAGATCAAAGGCTTA-3') and the reverse primer Actin2-3' (5'-ACTAAAACGCAAAACGAAAGCGGTT-3') amplified a 220 bp fragment of ACT2 cDNA and used as a semi-quantitative control [77]. For controls, 25 cycles of PCR were conducted (30 s at 94°C, 30 s at 55°C, 1 min at 72°C) in a final volume of 20 μL containing 2 μL cDNA, 1 mM dNTPs, 5 mM MgCl₂, 0.3 μM Actin forward/Actin reverse primers and 0.5 units of Taq DNA polymerase (Life Technologies) in the reaction buffer provided. For quantification of the PME1 transgene 30 cycles of PCR were conducted as described above using PMEfor/PMErev primers. The latter reaction was also used to confirm presence of the transgene following Basta selection.

Authors' contributions

PD conducted all of the experiments and wrote drafts of the manuscript. MCM helped supervise the project. PD, MCM and KR co-wrote the manuscript. KR oversaw the project in his lab and is the guarantor of the work.

Acknowledgements

The authors thank Nick Harberd (JIC) for ga1-3 and gai seeds, John Doonan (JIC) for pL4 vector, AlcR line P5-3 and advice on its use, Phil Mullineaux (JIC) for pGreen0229 vector, and Kirk Schnorr (Novozymes A/S, Bagsvaerd, Denmark) for the Aspergillus aculeatus PME1 clone. We thank Nick Harberd (JIC), Alistair MacDougall (IFR) and Benoit Menand (JIC) for constructive comments on the manuscript. PD was funded by a Biotechnology and Biological Sciences Research Council (BBSRC) studentship. PD and KR also received support from EU EDEN grant no QLK5-CT-2001-00443. KR was funded by BBSRC; MCM was funded by a Royal Society University Research Fellowship.

References

Carpita NC, Gibeaut DM. Structural models of primary cell walls in flowering plants - consistency of molecular structure with the physical properties of the walls during growth. Plant J. 1993;3:1–30. doi: 10.1046/j.1365-313X.1993.00999.x. [PubMed] [Cross Ref]
McCann MC, Roberts K. Architecture of the primary cell wall. In: Lloyd C, editor. The Cytoskeletal Basis of Plant Growth and Form. London , Academic Press; 1991. pp. 109–129.
Green PB. Organogenesis - a biophysical view. Annu Rev Plant Physiol Plant Molec Biol. 1980;31:51–82. doi: 10.1146/annurev.pp.31.060180.000411. [Cross Ref]
Brummell DA, Lashbrook CC, Bennett AB. Plant endo-1,4-beta-D-glucanases - structure, properties, and physiological function. (Acs Symposium Series).Enzymatic Conversion of Biomass for Fuels Production. 1994;566:100–129.
Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ. Xyloglucan Endotransglycosylase, a new wall-loosening enzyme- activity from plants. Biochem J. 1992;282:821–828. [PubMed]
Nishitani K, Tominaga R. Endoxyloglucan Transferase, a novel class of gycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule. J Biol Chem. 1992;267:21058–21064. [PubMed]
McQueen-Mason S, Cosgrove DJ. Disruption of hydrogen-bonding between plant cell wall polymers by proteins that induce wall extension. Proc Natl Acad Sci U S A. 1994;91:6574–6578. doi: 10.1073/pnas.91.14.6574. [PubMed] [Cross Ref]
Micheli F. Pectin methylesterases: cell wall enzymes with important roles in plant physiology. Trends Plant Sci. 2001;6:414–419. doi: 10.1016/S1360-1385(01)02045-3. [PubMed] [Cross Ref]
Jarvis MC. Structure and properties of pectin gels in plant cell walls. Plant Cell Environ. 1984;7:153–164.
Morris ER, Powell DA, Gidley MJ, Rees DA. Conformations and interactions of pectins .1. Polymorphism between gel and solid states of calcium polygalacturonate. J Mol Biol. 1982;155:507–516. doi: 10.1016/0022-2836(82)90484-3. [PubMed] [Cross Ref]
MacDougall AJ, Rigby NM, Ryden P, Tibbits CW, Ring SG. Swelling behavior of the tomato cell wall network. Biomacromolecules. 2001;2:450–455. doi: 10.1021/bm005632s. [PubMed] [Cross Ref]
Willats WGT, Orfila C, Limberg G, Buchholt HC, van Alebeek G, Voragen AGJ, Marcus SE, Christensen T, Mikkelsen JD, Murray BS, Knox JP. Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls - Implications for pectin methyl esterase action, matrix properties, and cell adhesion. J Biol Chem. 2001;276:19404–19413. doi: 10.1074/jbc.M011242200. [PubMed] [Cross Ref]
Ryden P, Sugimoto-Shirasu K, Smith AC, Findlay K, Reiter WD, McCann MC. Tensile properties of Arabidopsis cell walls depend on both a xyloglucan cross-linked microfibrillar network and rhamnogalacturonan II-borate complexes. Plant Physiol. 2003;132:1033–1040. doi: 10.1104/pp.103.021873. [PubMed] [Cross Ref]
Ruge U. Untersuchungen uber die anderungen der osmotischen zustandsgrossen und der membraneigen schaften des hypokotyls von Helianthus annuus beim normalen streckungswachstum. Planta. 1937;27:352–399. doi: 10.1007/BF01916877. [Cross Ref]
Goldberg R, Morvan C, Roland JC. Composition, properties and localization of pectins in young and mature cells of the mung bean hypocotyl. Plant Cell Physiol. 1986;27:417–429.
Bordenave M, Goldberg R. Immobilized and free apoplastic pectinmethylesterases in mung mean hypocotyl. Plant Physiol. 1994;106:1151–1156. [PubMed]
Kim JB, Carpita NC. Changes in esterification of the uronic acid groups of cell wall polysaccharides during elongation of maize coleoptiles. Plant Physiol. 1992;98:646–653. [PubMed]
McCann MC, Shi J, Roberts K, Carpita NC. Changes in pectin structure and localization during the growth of unadapted and NaCl-adapted tobacco cells. Plant J. 1994;5:773–785. doi: 10.1046/j.1365-313X.1994.5060773.x. [Cross Ref]
Pilling J, Willmitzer L, Fisahn J. Expression of a Petunia inflata pectin methyl esterase in Solanum tuberosum L. enhances stem elongation and modifies cation distribution. Planta. 2000;210:391–399. doi: 10.1007/PL00008147. [PubMed] [Cross Ref]
Wen FS, Zhu YM, Hawes MC. Effect of pectin methylesterase gene expression on pea root development. Plant Cell. 1999;11:1129–1140. doi: 10.1105/tpc.11.6.1129. [PubMed] [Cross Ref]
Hasunuma T, Fukusaki E, Kobayashi A. Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration in cell wall metabolism and a dwarf phenotype. J Biotechnol. 2004;111:241–251. doi: 10.1016/j.jbiotec.2004.04.015. [PubMed] [Cross Ref]
Sidler M, Hassa P, Hasan S, Ringli C, Dudler R. Involvement of an ABC transporter in a developmental pathway regulating hypocotyl cell elongation in the light. Plant Cell. 1998;10:1623–1636. doi: 10.1105/tpc.10.10.1623. [PubMed] [Cross Ref]
Collett CE, Harberd NP, Leyser O. Hormonal interactions in the control of Arabidopsis hypocotyl elongation. Plant Physiol. 2000;124:553–561. doi: 10.1104/pp.124.2.553. [PubMed] [Cross Ref]
Raz V, Koornneef M. Cell division activity during apical hook development. Plant Physiol. 2001;125:219–226. doi: 10.1104/pp.125.1.219. [PubMed] [Cross Ref]
Saibo NJM, Vriezen WH, Beemster GTS, Van der Straeten D. Growth and stomata development of Arabidopsis hypocotyls are controlled by gibberellins and modulated by ethylene and auxins. Plant J. 2003;33:989–1000. doi: 10.1046/j.1365-313X.2003.01684.x. [PubMed] [Cross Ref]
Gendreau E, Traas J, Desnos T, Grandjean O, Caboche M, Hofte H. Cellular basis of hypocotyl growth in Arabidopsis thaliana. Plant Physiol. 1997;114:295–305. doi: 10.1104/pp.114.1.295. [PubMed] [Cross Ref]
Sun TP, Goodman HM, Ausubel FM. Cloning the Arabidopsis Ga1 locus by genomic subtraction. Plant Cell. 1992;4:119–128. doi: 10.1105/tpc.4.2.119. [PubMed] [Cross Ref]
Sun TP, Kamiya Y. The Arabidopsis Ga1 locus encodes the cyclase ent-kaurene synthetase-a of gibberellin biosynthesis. Plant Cell. 1994;6:1509–1518. doi: 10.1105/tpc.6.10.1509. [PubMed] [Cross Ref]
Koornneef M, Vanderveen JH. Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L) Heynh. Theor Appl Genet. 1980;58:257–263. doi: 10.1007/BF00265176. [Cross Ref]
King KE, Moritz T, Harberd NP. Gibberellins are not required for normal stem growth in Arabidopsis thaliana in the absence of GAI and RGA. Genetics. 2001;159:767–776. [PubMed]
Koornneef M, Elgersma A, Hanhart CJ, Vanloenenmartinet EP, Vanrijn L, Zeevaart JAD. A gibberellin insensitive mutant of Arabidopsis thaliana. Physiol Plant. 1985;65:33–39. doi: 10.1111/j.1399-3054.1985.tb02355.x. [Cross Ref]
Fleck B, Harberd NP. Evidence that the Arabidopsis nuclear gibberellin signalling protein GAI is not destabilised by gibberellin. Plant J. 2002;32:935–947. doi: 10.1046/j.1365-313X.2002.01478.x. [PubMed] [Cross Ref]
Harberd NP, King KE, Carol P, Cowling RJ, Peng JR, Richards DE. Gibberellin: inhibitor of an inhibitor of ... ? Bioessays. 1998;20:1001–1008. doi: 10.1002/(SICI)1521-1878(199812)20:12<1001::AID-BIES6>3.0.CO;2-O. [PubMed] [Cross Ref]
Richards DE, King KE, Ait-ali T, Harberd NP. How gibberellin regulates plant growth and development: A molecular genetic analysis of gibberellin signaling. Annu Rev Plant Physiol Plant Molec Biol. 2001;52:67–88. doi: 10.1146/annurev.arplant.52.1.67. [PubMed] [Cross Ref]
Peng JR, Carol P, Richards DE, King KE, Cowling RJ, Murphy GP, Harberd NP. The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses. Genes Dev. 1997;11:3194–3205. [PubMed]
Cowling RJ, Harberd NP. Gibberellins control Arabidopsis hypocotyl growth via regulation of cellular elongation. J Exp Bot. 1999;50:1351–1357. doi: 10.1093/jexbot/50.337.1351. [Cross Ref]
Cowling RJ. Characterization of gibberellin responses in Arabidopsis thaliana seedlings. PhD thesis. 1997.
Chen LM, Carpita NC, Reiter WD, Wilson RH, Jeffries C, McCann MC. A rapid method to screen for cell-wall mutants using discriminant analysis of Fourier transform infrared spectra. Plant J. 1998;16:385–392. doi: 10.1046/j.1365-313x.1998.00301.x. [PubMed] [Cross Ref]
McCann MC, Chen L, Roberts K, Kemsley EK, Sene C, Carpita NC, Stacey NJ, Wilson RH. Infrared microspectroscopy: Sampling heterogeneity in plant cell wall composition and architecture. Physiol Plant. 1997;100:729–738. doi: 10.1111/j.1399-3054.1997.tb03080.x. [Cross Ref]
Schindelman G, Morikami A, Jung J, Baskin TI, Carpita NC, Derbyshire P, McCann MC, Benfey PN. COBRA encodes a putative GPI-anchored protein, which is polarly localized and necessary for oriented cell expansion in Arabidopsis. Genes Dev. 2001;15:1115–1127. doi: 10.1101/gad.879101. [PubMed] [Cross Ref]
Filippov MP, Kohn R. Determination of esterification degree of carboxyl groups of pectin with methanol by means of infrared spectroscopy. Chemicke Zvesti. 1975;29:88–91.
Carbohydrate Active Enzymes database http://afmb.cnrs-mrs.fr/CAZY/
Hou WC, Chang WH. Pectinesterase-catalyzed firming effects during precooking of vegetables. J Food Biochem. 1997;20:397–416.
Hwang JY, Lee CW, Wu MC, Chang HM. Transacylation and de-esterification reactions of pectin as catalyzed by pectinesterases from tomato and citrus. J Agric Food Chem. 2003;51:6287–6292. doi: 10.1021/jf020929y. [PubMed] [Cross Ref]
Maldonado MC, Desaad AMS, Callieri D. Purification and characterization of pectinesterase produced by a strain of Aspergillus niger. Curr Microbiol. 1994;28:193–196. doi: 10.1007/BF01575960. [Cross Ref]
Shevchik VE, Condemine G, Hugouvieux-Cotte-Pattat N, Robert-Baudouy J. Characterization of pectin methylesterase B, an outer membrane lipoprotein of Erwinia chrysanthemi 3937. Mol Microbiol. 1996;19:455–466. doi: 10.1046/j.1365-2958.1996.389922.x. [PubMed] [Cross Ref]
Christgau S, Kofod LV, Halkier T, Andersen LN, Hockauf M, Dorreich K, Dalboge H, Kauppinen S. Pectin methyl esterase from Aspergillus aculeatus: Expression cloning in yeast and characterization of the recombinant enzyme. Biochem J. 1996;319:705–712. [PubMed]
Roslan HA, Salter MG, Wood CD, White MRH, Croft KP, Robson F, Coupland G, Doonan J, Laufs P, Tomsett AB, Caddick MX. Characterization of the ethanol-inducible alc gene-expression system in Arabidopsis thaliana. Plant J. 2001;28:225–235. doi: 10.1046/j.1365-313X.2001.01146.x. [PubMed] [Cross Ref]
Francis KE, Lam SY, Copenhaver GP. Separation of Arabidopsis pollen tetrads is regulated by QUARTET1, a pectin methylesterase gene. Plant Physiol. 2006;142:1004–1013. doi: 10.1104/pp.106.085274. [PubMed] [Cross Ref]
Zhang GF, Staehelin LA. Functional compartmentation of the Golgi apparatus of plant cells - immunocytochemical analysis of high-pressure frozen-substituted and freeze-substituted sycamore maple suspension-culture cells. Plant Physiol. 1992;99:1070–1083. [PubMed]
Morris GA, Foster TJ, Harding SE. The effect of the degree of esterification on the hydrodynamic properties of citrus pectin. Food Hydrocolloids. 2000;14:227–235. doi: 10.1016/S0268-005X(00)00007-2. [Cross Ref]
Chanliaud E, Gidley MJ. In vitro synthesis and properties of pectin/Acetobacter xylinus cellulose composites. Plant J. 1999;20:25–35. doi: 10.1046/j.1365-313X.1999.00571.x. [PubMed] [Cross Ref]
Kacurakova M, Smith AC, Gidley MJ, Wilson RH. Molecular interactions in bacterial cellulose composites studied by 1D FT-IR and dynamic 2D FT-IR spectroscopy. Carbohydr Res. 2002;337:1145–1153. doi: 10.1016/S0008-6215(02)00102-7. [PubMed] [Cross Ref]
Wilson RH, Smith AC, Kacurakova M, Saunders PK, Wellner N, Waldron KW. The mechanical properties and molecular dynamics of plant cell wall polysaccharides studied by Fourier-transform infrared spectroscopy. Plant Physiol. 2000;124:397–405. doi: 10.1104/pp.124.1.397. [PubMed] [Cross Ref]
Limberg G, Korner R, Buchholt HC, Christensen T, Roepstorff P, Mikkelsen JD. Analysis of pectin structure part 1 - Analysis of different de-esterification mechanisms for pectin by enzymatic fingerprinting using endopectin lyase and endopolygalacturonase II from A. niger. Carbohydr Res. 2000;327:293–307. doi: 10.1016/S0008-6215(00)00067-7. [PubMed] [Cross Ref]
Limberg G, Korner R, Buchholt HC, Christensen T, Roepstorff P, Mikkelsen JD. Analysis of pectin structure part 3 - Quantification of the amount of galacturonic acid residues in blocksequences in pectin homogalacturonan by enzymatic fingerprinting with exo- and endo-polygalacturonase II from Aspergillus niger. Carbohydr Res. 2000;327:321–332. doi: 10.1016/S0008-6215(00)00068-9. [PubMed] [Cross Ref]
Liners F, Thibault JF, Van Cutsem P. Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pectin on their recognition by monoclonal-antibodies. Plant Physiol. 1992;99:1099–1104. [PubMed]
Zwieniecki MA, Melcher PJ, Holbrook NM. Hydrogel control of xylem hydraulic resistance in plants. Science. 2001;291:1059–1062. doi: 10.1126/science.1057175. [PubMed] [Cross Ref]
Savaldi-Goldstein S, Peto C, Chory J. The epidermis both drives and restricts plant shoot growth. Nature. 2007;446:199–202. doi: 10.1038/nature05618. [PubMed] [Cross Ref]
Derbyshire P, Findlay K, McCann MC, Roberts K. Cell elongation in Arabidopsis hypocotyls involves dynamic changes in cell wall thickness. J Exp Bot. 2007;doi: 10.1093/jxb/erm074
Giovane A, Servillo L, Balestrieri C, Raiola A, D'Avino R, Tamburrini M, Clardiello MA, Camardella L. Pectin methylesterase inhibitor. BBA-Proteins Proteomics. 2004;1696:245–252. doi: 10.1016/j.bbapap.2003.08.011. [Cross Ref]
Raiola A, Camardella L, Giovane A, Mattei B, De Lorenzo G, Cervone F, Bellincampi D. Two Arabidopsis thaliana genes encode functional pectin methylesterase inhibitors. FEBS Lett. 2004;557:199–203. doi: 10.1016/S0014-5793(03)01491-1. [PubMed] [Cross Ref]
Wolf S, Grsic-Rausch S, Rausch T, Greiner S. Identification of pollen-expressed pectin methylesterase inhibitors in Arabidopsis. FEBS Lett. 2003;555:551–555. doi: 10.1016/S0014-5793(03)01344-9. [PubMed] [Cross Ref]
Lionetti V, Raiola A, Camardella L, Giovane A, Obel N, Pauly M, Favaron F, Cervone F, Bellincampi D. Overexpression of Pectin Methylesterase Inhibitors in Arabidopsis Restricts Fungal Infection by Botrytis cinerea. Plant Physiol. 2007;143:1871–1880. doi: 10.1104/pp.106.090803. [PubMed] [Cross Ref]
Carpita NC, Kanabus J. Chemical structure of the cell walls of dwarf maize and changes mediated by gibberellin. Plant Physiol. 1988;88:671–678. [PubMed]
Chen L, Higashitani A, Suge H, Takeda K, Takahashi H. Spiral growth and cell wall properties of the gibberellin-treated first internodes in the seedlings of a wheat cultivar tolerant to deep sowing conditions. Physiol Plant. 2003;118:147–155. doi: 10.1034/j.1399-3054.2003.00093.x. [PubMed] [Cross Ref]
Chen F, Nonogaki H, Bradford KJ. A gibberellin-regulated xyloglucan endotransglycosylase gene is expressed in the endosperm cap during tomato seed germination. J Exp Bot. 2002;53:215–223. doi: 10.1093/jexbot/53.367.215. [PubMed] [Cross Ref]
Keyes G, Sorrells ME, Setter TL. Gibberellic acid regulates cell wall extensibility in wheat (Triticum aestivum L.) Plant Physiol. 1990;92:242–245. [PubMed]
Jones RL. The role of gibberellins in plant cell elongation. CRC Critical Reviews in Plant Sciences. 1983;1:23–47.
Taylor A, Cosgrove DJ. Gibberellic acid stimulation of cucumber hypocotyl elongation - effects on growth, turgor, osmotic pressure, and cell wall properties. Plant Physiol. 1989;90:1335–1340. [PubMed]
Kawamura H, Kamisaka S, Masuda Y. Regulation of lettuce hypocotyl elongation by gibberellic acid - correlation between cell elongation, stress-relaxation properties of cell wall and wall polysaccharide content. Plant Cell Physiol. 1976;17:23–34.
Montague MJ. Gibberellic acid promotes growth and cell wall synthesis in Avena internodes regardless of the orientation of cell expansion. Physiol Plant. 1995;94:7–18. doi: 10.1111/j.1399-3054.1995.tb00777.x. [Cross Ref]
Scheres B, Wolkenfelt H, Willemsen V, Terlouw M, Lawson E, Dean C, Weisbeek P. Embryonic origin of the Arabidopsis primary root and root meristem initials. Development. 1994;120:2475–2487.
Blumenkrantz N, Asboe-Hansen G. New method for quantitative determination of uronic acids. Analytical Biochemistry. 1973;54:484–489. doi: 10.1016/0003-2697(73)90377-1. [PubMed] [Cross Ref]
Armitage P, Berry G. Statistical Methods in Medical Research. 2nd. Oxford , Blackwell Scientific Publications; 1987. pp. 90–92.
Clough SJ, Bent AF. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998;16:735–743. doi: 10.1046/j.1365-313x.1998.00343.x. [PubMed] [Cross Ref]
Laval V, Koroleva OA, Murphy E, Lu CG, Milner JJ, Hooks MA, Tomos AD. Distribution of actin gene isoforms in the Arabidopsis leaf measured in microsamples from intact individual cells. Planta. 2002;215:287–292. doi: 10.1007/s00425-001-0732-y. [PubMed] [Cross Ref]

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