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J Virol. May 1996; 70(5): 3302–3306.
PMCID: PMC190198
Human endogenous retrovirus K10 encodes a functional integrase.
Y Kitamura, T Ayukawa, T Ishikawa, T Kanda, and K Yoshiike
Division of Molecular Genetics, National Institute of Health, Tokyo, Japan.
Abstract
We cloned a human endogenous retrovirus K1O DNA fragment encoding integrase and expressed it as a fusion protein with Escherichia coli maltose-binding protein. Integrase activities were measured in vitro by using a double-stranded oligonucleotide as a substrate mimicking viral long terminal repeats (LTR). The fusion protein was highly active for both terminal cleavage and strand transfer in the presence of Mn2+ on the K1O LTR substrate. It was also active on both Rous sarcoma virus and human immunodeficiency virus type 1 LTR substrates, whereas Rous sarcoma virus and human immunodeficiency virus type 1 integrases were active only on their corresponding LTR substrates. The results strongly suggest that K1O encodes a functional integrase with relaxed substrate specificity.
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