Search tips
Search criteria 


Logo of molcellbPermissionsJournals.ASM.orgJournalMCB ArticleJournal InfoAuthorsReviewers
Published online 2007 February 16. doi: 10.1128/MCB.00064-07

FIG. 5.

An external file that holds a picture, illustration, etc.
Object name is zmb0080767070005.jpg

Mak11 and Rlp24 are functionally and physically linked. (A) RLP24 is a high-copy-number suppressor of the mak11-2 ts phenotype. Rlp24 was expressed from a high-copy-number vector (pCM190-RLP24) under the control of a tetracycline-repressible promoter in wild-type or mak11-2 cells at permissive and nonpermissive temperatures. Growth was estimated by 10-fold serial dilutions on solid yeast extract-peptone-dextrose medium with or without doxycycline. (B) Protein-protein interaction between (His)6-Mak11 and GST-Rlp24 was detected by mixing total extracts of E. coli overexpressing the proteins and pulling down the GST fusion proteins with glutathione-Sepharose. Eluates were separated on 4 to 12% Novex polyacrylamide gels and Coomassie blue stained. Immunoblot assays were performed in parallel with rabbit polyclonal antibodies raised against Mak11. Asterisks indicate GST-tagged proteins. Rpl3 and Rpl5 were used as controls.

Images in this article

  • FIG. 1.
  • FIG. 2.
  • FIG. 3.
  • FIG. 4.
  • FIG. 5.
  • FIG. 6.
  • FIG. 7.
  • FIG. 8.
Click on the image to see a larger version.